##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936688.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	265919
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.037936364080792	31.0	31.0	33.0	28.0	34.0
2	31.058634396188314	31.0	31.0	34.0	28.0	34.0
3	30.848803583045964	31.0	30.0	34.0	27.0	34.0
4	34.85626450159635	35.0	35.0	37.0	32.0	37.0
5	35.07646313351058	35.0	35.0	37.0	33.0	37.0
6	35.04579966079896	35.0	35.0	37.0	32.0	37.0
7	31.051594658523836	35.0	35.0	37.0	0.0	37.0
8	32.957299027147364	35.0	35.0	37.0	17.0	37.0
9	36.0036853327517	38.0	34.0	39.0	32.0	39.0
10	35.985130810509965	37.0	35.0	39.0	32.0	39.0
11	36.25332150015606	37.0	35.0	39.0	32.0	39.0
12	36.31124891414303	38.0	35.0	39.0	32.0	39.0
13	36.102049872329545	38.0	35.0	39.0	31.0	39.0
14	37.18721490378649	39.0	36.0	40.0	32.0	41.0
15	37.29749660610938	39.0	36.0	40.0	32.0	41.0
16	37.385015737875065	39.0	36.0	40.0	32.0	41.0
17	37.18412373692741	39.0	35.0	40.0	32.0	41.0
18	37.10038019096041	39.0	36.0	40.0	32.0	41.0
19	36.873431383240764	39.0	35.0	40.0	31.0	41.0
20	36.78397557150862	39.0	35.0	40.0	31.0	41.0
21	36.87243107863673	39.0	35.0	40.0	31.0	41.0
22	36.96991565100651	39.0	35.0	40.0	32.0	41.0
23	37.0548700920205	39.0	35.0	40.0	32.0	41.0
24	36.93353991252975	39.0	35.0	40.0	31.0	41.0
25	36.71834656417932	38.0	35.0	40.0	31.0	41.0
26	36.61824841399073	38.0	35.0	40.0	31.0	41.0
27	36.66619158465548	38.0	35.0	40.0	31.0	41.0
28	36.60777153945374	38.0	35.0	40.0	31.0	41.0
29	36.60474430183627	38.0	35.0	40.0	31.0	41.0
30	36.417168385861864	38.0	35.0	40.0	30.0	41.0
31	36.40713901601615	38.0	35.0	40.0	30.0	41.0
32	36.19713521786709	38.0	35.0	40.0	30.0	41.0
33	36.018031806677975	38.0	35.0	40.0	30.0	41.0
34	35.927853970569984	38.0	35.0	40.0	29.0	41.0
35	35.78356943279721	38.0	35.0	40.0	28.0	41.0
36	35.750115636716444	38.0	35.0	40.0	28.0	41.0
37	35.71862108386388	38.0	35.0	40.0	28.0	41.0
38	35.55997126944671	38.0	35.0	40.0	27.0	41.0
39	35.57559632820521	38.0	35.0	40.0	27.0	41.0
40	35.340411929948594	38.0	34.0	40.0	26.0	41.0
41	35.21379818666586	38.0	34.0	40.0	26.0	41.0
42	35.24916233890771	38.0	34.0	40.0	26.0	41.0
43	35.25309586753861	38.0	34.0	40.0	26.0	41.0
44	35.07378186590653	38.0	34.0	40.0	25.0	41.0
45	35.00406890820137	38.0	34.0	40.0	24.0	41.0
46	34.875416950274335	38.0	34.0	40.0	24.0	41.0
47	34.81542123729406	38.0	34.0	40.0	24.0	41.0
48	34.74470797498486	37.0	34.0	40.0	24.0	41.0
49	34.65958054896416	37.0	34.0	40.0	24.0	41.0
50	34.455225087338626	37.0	34.0	40.0	23.0	41.0
51	32.69762972935368	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	11.0
10	18.0
11	11.0
12	14.0
13	10.0
14	7.0
15	16.0
16	39.0
17	80.0
18	147.0
19	286.0
20	460.0
21	756.0
22	1068.0
23	1535.0
24	2188.0
25	3083.0
26	3971.0
27	4389.0
28	4815.0
29	5258.0
30	6547.0
31	8387.0
32	10680.0
33	14670.0
34	22161.0
35	23951.0
36	25532.0
37	37463.0
38	49457.0
39	38897.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.37051132111658	18.09799224575905	20.937954790744552	14.593541642379822
2	31.870607214978996	29.757557752548706	22.210522753169197	16.161312279303097
3	29.625938725702188	27.86036349414671	27.238745633068717	15.274952147082383
4	27.37450125790184	22.796791504179843	32.41626209484843	17.41244514306988
5	28.569978076030672	26.710389253870538	25.456624009566823	19.263008660531966
6	24.340494661908327	36.40995942373429	26.082754522993845	13.166791391363535
7	63.12786976485321	17.07474832561795	15.93755993366401	3.859821975864831
8	70.77418311591124	10.61864703161489	13.045325832302318	5.561844020171556
9	65.39096491788852	8.484914579251576	13.850834276603024	12.273286226256868
10	39.71660543248132	24.460456003519866	21.401629819606722	14.421308744392089
11	32.50801935927858	23.95015023371779	25.72362260688405	17.818207800119584
12	29.652262531071493	21.56370924980915	30.000488870671145	18.783539348448212
13	23.584625393446874	24.67142250083672	33.09127967538987	18.65267243032653
14	18.046096743745277	30.878199752556228	29.641733008923772	21.433970494774723
15	16.955915147093663	25.85561768809299	39.89147071100598	17.296996453807363
16	19.736085048454605	24.872611584730688	32.936721332435816	22.454582034378888
17	19.371312316908533	25.042964210906327	32.316607688807494	23.269115783377643
18	20.634478920272713	25.374268104197146	32.41438182303634	21.576871152493805
19	22.512870460553778	27.831783362602895	27.522666676694783	22.13267950014854
20	24.765812145803796	25.775894163260244	32.57758941632603	16.880704274609936
21	22.09206562900733	30.063289949195056	29.040422083416377	18.804222338381233
22	20.138839270604958	24.780102211575706	30.13586844114185	24.945190076677484
23	19.933513588724384	29.00695324516112	30.56231408812458	20.497219077989914
24	22.170284936390406	23.10628424445038	30.922198112959208	23.80123270620001
25	19.491273658520075	29.7876421015422	28.658726905561466	22.062357334376255
26	18.285643372605946	27.0950928666248	29.496199970667757	25.123063790101497
27	21.454277430345332	28.03447666394654	29.034781267980097	21.47646463772803
28	16.6276196887022	26.085010849168356	35.15506601634332	22.13230344578612
29	20.912383094100086	23.708347278682606	29.618041584091397	25.761228043125918
30	18.65229637596411	26.579522335748855	34.28337200425693	20.4848092840301
31	21.949541025650667	24.125767620967288	30.71273583309203	23.21195552029001
32	23.70947544176986	26.58215471628579	27.973179802872306	21.735190039072048
33	20.606274843091317	24.609749585400063	30.35097153644531	24.43300403506331
34	20.80332732899868	24.123511294792777	31.934160402227747	23.1390009739808
35	21.324538675310905	23.431947322304914	30.073819471342777	25.169694531041408
36	20.17982919610859	25.150891812920477	30.414148669331638	24.255130321639296
37	19.76128069073665	25.121935627014242	33.804654800898014	21.31212888135109
38	19.285195867914666	25.93233277802639	30.8533801646366	23.929091189422344
39	21.529488302829055	23.13035172364517	31.703263023702704	23.636896949823065
40	23.7485850954614	22.535809776661313	33.15671313445072	20.55889199342657
41	18.18824529273952	25.763108314938005	30.557801435775556	25.490844956546923
42	22.30904899612288	23.98136274579853	30.34570677537145	23.363881482707143
43	22.56100541894336	23.591394371970413	27.86036349414671	25.98723671493951
44	20.677349117588438	25.458128227016495	30.155047213625203	23.70947544176986
45	19.93238542563713	23.94187703774458	28.853147010931902	27.27259052568639
46	23.326652100827697	26.81982107333436	27.486565457902596	22.36696136793535
47	18.604537471936943	23.566574784050783	35.12310139553774	22.705786348474536
48	20.573558113560896	24.216020667947756	29.845930527717083	25.36449069077426
49	19.50142712630538	22.11312467330277	33.86745587942193	24.51799232096992
50	20.002707591409415	22.55348433169499	32.07142024451055	25.37238783238505
51	18.91327810348264	23.64140960217209	28.69144363509189	28.753868659253385
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	84.0
1	253.5
2	423.0
3	1967.5
4	3512.0
5	2492.5
6	1473.0
7	1399.0
8	1325.0
9	1272.0
10	1219.0
11	1180.5
12	1142.0
13	1069.0
14	996.0
15	961.0
16	926.0
17	921.0
18	916.0
19	934.5
20	953.0
21	958.0
22	963.0
23	1104.5
24	1246.0
25	1386.0
26	1844.0
27	2162.0
28	2703.5
29	3245.0
30	4234.0
31	5223.0
32	5710.0
33	6197.0
34	6674.0
35	7151.0
36	7635.5
37	8120.0
38	8811.0
39	9502.0
40	10637.5
41	11773.0
42	13985.0
43	16197.0
44	18018.0
45	19839.0
46	28611.5
47	37384.0
48	30889.0
49	24394.0
50	23218.5
51	22043.0
52	18333.0
53	14623.0
54	13406.5
55	12190.0
56	11451.0
57	10712.0
58	10070.0
59	9428.0
60	9641.0
61	9854.0
62	8960.5
63	8067.0
64	6212.5
65	4358.0
66	3493.5
67	2629.0
68	2204.0
69	1779.0
70	1379.0
71	979.0
72	835.5
73	692.0
74	474.0
75	231.5
76	207.0
77	151.0
78	95.0
79	83.0
80	71.0
81	45.5
82	20.0
83	14.5
84	9.0
85	8.0
86	7.0
87	6.0
88	5.0
89	2.5
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	265919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.01035653714101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.65678903140204	25.731143694132424
2	8.971693940734188	6.102610193329547
3	3.4995577178239716	3.570636171164904
4	1.9736842105263157	2.685028147669027
5	1.3567005749668288	2.3070935134383026
6	0.9287925696594427	1.8953139865899014
7	0.7109685979655019	1.6926206852462593
8	0.583812472357364	1.5884536268562983
9	0.4522335249889429	1.3842561080629816
>10	5.728659885006635	41.752939804978205
>50	0.0950906678460858	2.105904429544335
>100	0.030959752321981428	1.9566108476641384
>500	0.005528527200353826	1.2458681026929252
>1k	0.005528527200353826	5.981520688630749
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	4421	1.6625363362527685	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	4360	1.6395970201452323	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	3567	1.3413859107472577	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1780	0.6693767651051636	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1778	0.6686246563803263	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	888	0.33393627382774455	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	657	0.2470677161090407	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	616	0.23164948724987683	TruSeq Adapter, Index 27 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	588	0.22111996510215517	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTA	564	0.21209466040410802	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	453	0.17035262617563995	TruSeq Adapter, Index 27 (95% over 24bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	411	0.15455834295405743	No Hit
GAATCTATCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	395	0.14854147315535934	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	382	0.14365276644391714	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTT	295	0.11093603691349621	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	279	0.10491916711479812	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7605436241863125E-4	0.0	0.0	0.20043697516913045	0.0
2	3.7605436241863125E-4	0.0	0.0	1.4241178704793565	0.0
3	3.7605436241863125E-4	0.0	0.0	1.6911164677965846	0.0
4	3.7605436241863125E-4	0.0	0.0	2.286410523505278	0.0
5	3.7605436241863125E-4	0.0	0.0	4.625468657749164	0.0
6	3.7605436241863125E-4	0.0	0.0	5.292213042317397	0.0
7	3.7605436241863125E-4	0.0	0.0	6.150745151719133	0.0
8	3.7605436241863125E-4	0.0	0.0	7.273267423538747	0.0
9	3.7605436241863125E-4	0.0	0.0	7.572982750386396	0.0
10	3.7605436241863125E-4	0.0	0.0	10.16813390543737	0.0
11	3.7605436241863125E-4	0.0	0.0	11.112782463832971	0.0
12	3.7605436241863125E-4	0.0	0.0	13.737641913515018	0.0
13	3.7605436241863125E-4	0.0	0.0	14.161079125598397	0.0
14	3.7605436241863125E-4	0.0	0.0	14.373925894727341	0.0
15	3.7605436241863125E-4	0.0	0.0	14.925221590033056	0.0
16	3.7605436241863125E-4	0.0	0.0	15.501336873258397	0.0
17	3.7605436241863125E-4	0.0	0.0	16.118066027624952	0.0
18	3.7605436241863125E-4	0.0	0.0	16.816022924273934	0.0
19	3.7605436241863125E-4	0.0	0.0	17.289099312196573	0.0
20	3.7605436241863125E-4	0.0	0.0	17.738108220924417	0.0
21	3.7605436241863125E-4	0.0	0.0	18.224722565894126	0.0
22	3.7605436241863125E-4	0.0	0.0	18.746309966568766	0.0
23	3.7605436241863125E-4	0.0	0.0	19.213369484692706	0.0
24	7.521087248372625E-4	0.0	0.0	19.642071457849948	0.0
25	7.521087248372625E-4	0.0	0.0	19.985409090738155	0.0
26	7.521087248372625E-4	0.0	0.0	20.288508906847575	0.0
27	7.521087248372625E-4	0.0	0.0	20.632222594098202	0.0
28	0.0011281630872558937	0.0	0.0	20.967287031013203	0.0
29	0.0011281630872558937	0.0	0.0	21.340332958532485	0.0
30	0.0011281630872558937	0.0	0.0	21.74045480014591	0.0
31	0.0011281630872558937	0.0	0.0	22.136816098135146	0.0
32	0.0011281630872558937	0.0	0.0	22.497452231694613	0.0
33	0.0011281630872558937	0.0	0.0	22.816346331025613	0.0
34	0.0011281630872558937	0.0	0.0	23.175854301497825	0.0
35	0.0011281630872558937	0.0	0.0	23.560557914252083	0.0
36	0.0011281630872558937	0.0	0.0	23.9403728202949	0.0
37	0.0011281630872558937	0.0	0.0	24.313418747814183	0.0
38	0.0011281630872558937	0.0	0.0	24.706395556541654	0.0
39	0.0011281630872558937	0.0	0.0	25.08658651694689	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	45	3.8198777E-10	45.000004	13
CGGGTTG	20	7.023496E-4	45.000004	6
CAACGAG	45	3.8198777E-10	45.000004	14
TCCGATG	20	7.023496E-4	45.000004	14
GCGCGAG	20	7.023496E-4	45.000004	1
TCCTCAA	20	7.023496E-4	45.000004	39
TTATCGA	20	7.023496E-4	45.000004	14
CGTTAGG	20	7.023496E-4	45.000004	2
CCCGTTT	20	7.023496E-4	45.000004	30
AGACGCA	20	7.023496E-4	45.000004	36
TCGGAGC	20	7.023496E-4	45.000004	13
GTTAATT	20	7.023496E-4	45.000004	32
CGGGATC	20	7.023496E-4	45.000004	6
CTCGCAG	20	7.023496E-4	45.000004	1
GGTGACG	20	7.023496E-4	45.000004	21
CTCGAAG	20	7.023496E-4	45.000004	1
CAATGTC	20	7.023496E-4	45.000004	13
GGACACG	20	7.023496E-4	45.000004	1
CTCTCGC	20	7.023496E-4	45.000004	45
TCGACGG	20	7.023496E-4	45.000004	17
>>END_MODULE