FastQCFastQC Report
Sat 14 Jan 2017
SRR2936685.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936685.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268493
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC52821.9672766142878957No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG51061.9017255570908738No Hit
GAATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC42691.5899855862164005No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34031.2674445888719632No Hit
GCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC14340.5340921364802808TruSeq Adapter, Index 22 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCT10180.3791532740145926No Hit
GAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT8020.298704249272793No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTC7700.2867858752369708No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTA6280.23389809045300994No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC5130.19106643376177404No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG4900.18250010242352688No Hit
GAATCTATCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC4360.16238784623807698No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4330.16127049867221863No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3710.1381786489778132No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3100.11545924847202721No Hit
AGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAAGGAAGG2980.11098985820859389No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2830.10540312037930226No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTT2810.10465822200206337No Hit
CCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC2730.10167862849310783TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA253.882793E-545.00000413
CGGGTTG253.882793E-545.0000046
AGCGTGA253.882793E-545.00000428
TCGTTTC253.882793E-545.00000443
ACTATGC551.8189894E-1245.0000048
GGCACCG253.882793E-545.0000048
TAGCGAG253.882793E-545.0000041
GCACGTC253.882793E-545.00000439
TACATGC253.882793E-545.00000415
ACGGCCT253.882793E-545.0000048
TCTGTCG253.882793E-545.00000424
CTCGATA253.882793E-545.0000041
GCGGAGT253.882793E-545.0000049
CACGTCA253.882793E-545.00000440
CACAACG253.882793E-545.00000412
TAGTGAG253.882793E-545.0000041
TCGACAT253.882793E-545.00000413
CGTTTCG253.882793E-545.00000444
GACCTAT253.882793E-545.00000433
CGATAGC253.882793E-545.00000410
Produced by FastQC (version 0.11.5)