##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936685.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	268493
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.023892615449938	31.0	31.0	33.0	28.0	34.0
2	31.071256978766673	31.0	31.0	34.0	28.0	34.0
3	30.783968297125064	31.0	30.0	34.0	27.0	34.0
4	34.7791041107217	35.0	35.0	37.0	32.0	37.0
5	35.048016149396815	35.0	35.0	37.0	33.0	37.0
6	35.006651942508746	35.0	35.0	37.0	32.0	37.0
7	31.161438100807096	35.0	35.0	37.0	0.0	37.0
8	32.99074836215469	35.0	35.0	37.0	17.0	37.0
9	35.98077044839158	38.0	34.0	39.0	32.0	39.0
10	36.02238047174414	37.0	35.0	39.0	32.0	39.0
11	36.28154179066121	37.0	35.0	39.0	32.0	39.0
12	36.26675555787302	37.0	35.0	39.0	32.0	39.0
13	35.95416640284849	38.0	35.0	39.0	31.0	39.0
14	37.05587482727669	39.0	36.0	40.0	31.0	41.0
15	37.16483111291542	39.0	35.0	40.0	32.0	41.0
16	37.30565415113243	39.0	36.0	40.0	32.0	41.0
17	37.09779770794769	39.0	35.0	40.0	32.0	41.0
18	36.97514274115154	39.0	35.0	40.0	32.0	41.0
19	36.74274934542055	38.0	35.0	40.0	31.0	41.0
20	36.56801853307162	38.0	35.0	40.0	31.0	41.0
21	36.69796233048906	38.0	35.0	40.0	31.0	41.0
22	36.74289087611223	38.0	35.0	40.0	31.0	41.0
23	36.83857307266856	38.0	35.0	40.0	32.0	41.0
24	36.66251261671626	38.0	35.0	40.0	31.0	41.0
25	36.46911837552562	38.0	35.0	40.0	31.0	41.0
26	36.320459006380055	38.0	35.0	40.0	31.0	41.0
27	36.352791320444105	38.0	35.0	40.0	31.0	41.0
28	36.28249153609219	38.0	35.0	40.0	30.0	41.0
29	36.278774493189765	38.0	35.0	40.0	30.0	41.0
30	36.03443665197975	38.0	35.0	40.0	30.0	41.0
31	36.00830561690621	38.0	35.0	40.0	30.0	41.0
32	35.74178097753014	38.0	35.0	40.0	29.0	41.0
33	35.448834792713406	38.0	34.0	40.0	27.0	41.0
34	35.33953957831303	38.0	34.0	40.0	26.0	41.0
35	35.221089562856385	38.0	34.0	40.0	25.0	41.0
36	35.158726670713946	38.0	34.0	40.0	25.0	41.0
37	35.05960304365477	38.0	34.0	40.0	25.0	41.0
38	34.90958795946263	38.0	34.0	40.0	24.0	41.0
39	34.84877073145296	38.0	34.0	40.0	24.0	41.0
40	34.64510061714831	38.0	34.0	40.0	23.0	41.0
41	34.51775651506743	38.0	34.0	40.0	23.0	41.0
42	34.58810099332199	38.0	34.0	40.0	23.0	41.0
43	34.59660028380628	38.0	34.0	40.0	23.0	41.0
44	34.36542479692208	38.0	34.0	40.0	22.0	41.0
45	34.31254818561378	37.0	33.0	40.0	23.0	41.0
46	34.1479479911953	37.0	33.0	40.0	22.0	41.0
47	34.04522650497406	37.0	33.0	40.0	22.0	41.0
48	34.021482869199566	37.0	33.0	40.0	22.0	41.0
49	33.96368247961772	37.0	33.0	40.0	21.0	41.0
50	33.80319039974972	37.0	33.0	40.0	20.0	41.0
51	32.14204839604757	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	25.0
10	30.0
11	18.0
12	12.0
13	11.0
14	10.0
15	28.0
16	42.0
17	85.0
18	171.0
19	351.0
20	643.0
21	910.0
22	1351.0
23	1900.0
24	2701.0
25	3868.0
26	4818.0
27	5536.0
28	5645.0
29	6061.0
30	7145.0
31	8712.0
32	11544.0
33	15394.0
34	23650.0
35	25005.0
36	24794.0
37	35480.0
38	46442.0
39	36103.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.545693928705774	17.896555962352835	19.414658855165683	17.1430912537757
2	32.407176350966324	30.105812814486782	20.911159695038602	16.575851139508295
3	28.96723564487715	29.316592983802188	28.228668903844795	13.487502467475876
4	26.642035360325966	21.925338835649345	35.073912541481526	16.358713262543155
5	28.85810803261165	26.30496884462537	26.546315918850773	18.290607203912206
6	22.38978297385779	38.29187353115351	27.192887710294123	12.125455784694573
7	60.368799186570975	17.288718886525906	18.450015456641328	3.8924664702617946
8	67.36376739803272	12.287471181744031	15.357569843534097	4.99119157668915
9	62.31447374791894	8.151422942125121	16.131146808296677	13.402956501659261
10	40.033445937138026	22.318645178831478	23.268018160622436	14.379890723408058
11	33.09657979910091	23.116431340854323	27.2614183610001	16.52557049904467
12	28.803358001884593	19.82584275940155	32.10847210169352	19.262327137020332
13	22.48699221208747	26.123213640579085	35.338723914589956	16.0510702327435
14	19.432163967030796	30.768772370229392	30.017542356783977	19.781521305955835
15	16.880514575798998	23.887773610485187	42.30352374177353	16.928188071942284
16	18.16062243708402	24.431921875058197	34.22845288331539	23.17900280454239
17	18.02132644054035	24.38089633621733	33.53532494329461	24.062452279947706
18	19.849307058284573	24.929141541865153	33.79417712938512	21.42737427046515
19	22.445650352150707	26.462887300600013	30.19296592462373	20.89849642262554
20	24.54849847109608	25.787636921632966	33.50329431307334	16.160570294197615
21	21.68920605006462	30.323695589829157	30.72184377246334	17.265254587642882
22	18.991556576894	24.501942322518648	31.91554342198866	24.590957678598695
23	19.61838856134052	29.639878879523863	31.205655268479997	19.536077290655623
24	21.986792951771555	24.175304384099398	31.816099488627263	22.021803175501784
25	18.744622764839306	29.70096054645745	30.425001769133647	21.129414919569598
26	17.819086531119993	26.014830926690824	31.631364691072022	24.53471785111716
27	21.3536293311185	28.614898712443154	29.715486064813607	20.315985891624734
28	16.893922746589297	25.94555537760761	36.760362467550365	20.40015940825273
29	20.492899256218973	23.856860327829775	30.84884894578257	24.80139147016868
30	18.873862633290255	30.454425255034582	31.64067592078751	19.031036190887658
31	22.93281389086494	24.688166916828372	30.28086393313792	22.098155259168767
32	22.055323602477532	28.071867795436006	27.8647860465636	22.008022555522864
33	20.229950129053645	28.375786333349474	27.30313267012548	24.091130867471406
34	18.833265671730732	26.583560837712717	31.41422681410689	23.168946676449664
35	18.235484723996528	27.76161762131601	26.603673093898166	27.399224560789293
36	19.40944456652501	26.884499782117228	29.590343137437475	24.115712513920286
37	17.635096631941988	29.918470872611202	30.785905032905887	21.660527462540923
38	17.7732752809198	30.646609036362214	27.121749915267813	24.45836576745018
39	20.17892459021278	26.334392330526306	29.970241309829305	23.516441769431605
40	19.92491424357432	26.20142797018917	30.20078735758474	23.67287042865177
41	16.836193122353283	26.77797931417206	30.23728737806945	26.148540185405206
42	18.399734816177705	24.657998532550195	31.61199733326381	25.33026931800829
43	21.860160227640943	23.58050302987415	26.853586499461812	27.705750243023097
44	19.82472541183569	25.61742764243388	30.321833343886063	24.23601360184437
45	18.593035945071193	25.82488184049491	28.847307006141687	26.73477520829221
46	23.227421199062917	28.471505774824667	27.242051003191893	21.059022022920523
47	18.324127630887958	23.94066139526915	35.92272424234524	21.812486731497653
48	20.269429743047304	25.729534848208335	28.794046772169107	25.206988636575257
49	18.66864313036094	22.930579195733223	34.239253909785354	24.161523764120478
50	19.33607207636698	24.718707750295167	31.53676259716268	24.40845757617517
51	19.186719951730584	23.898574636955153	28.402602674930073	28.512102736384186
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	312.5
2	539.0
3	3283.5
4	6028.0
5	4130.5
6	2233.0
7	2033.5
8	1834.0
9	1784.5
10	1735.0
11	1699.0
12	1663.0
13	1587.5
14	1512.0
15	1464.5
16	1417.0
17	1307.0
18	1197.0
19	1200.5
20	1204.0
21	1120.0
22	1036.0
23	1125.0
24	1214.0
25	1505.0
26	1841.0
27	1886.0
28	2519.5
29	3153.0
30	3687.0
31	4221.0
32	5027.0
33	5833.0
34	6988.5
35	8144.0
36	8072.0
37	8000.0
38	8406.5
39	8813.0
40	10928.0
41	13043.0
42	14314.5
43	15586.0
44	17367.5
45	19149.0
46	30124.5
47	41100.0
48	32536.5
49	23973.0
50	22354.5
51	20736.0
52	17986.0
53	15236.0
54	13935.0
55	12634.0
56	11296.0
57	9958.0
58	9246.0
59	8534.0
60	8165.5
61	7797.0
62	6955.5
63	6114.0
64	5226.5
65	4339.0
66	3375.5
67	2412.0
68	2044.5
69	1677.0
70	1472.0
71	1267.0
72	905.5
73	544.0
74	436.0
75	299.0
76	270.0
77	188.0
78	106.0
79	95.0
80	84.0
81	56.5
82	29.0
83	19.5
84	10.0
85	8.5
86	7.0
87	7.0
88	7.0
89	6.5
90	6.0
91	4.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	268493.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.000487908437094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.96379395964065	25.39842751952565
2	8.567333303236873	5.654523581620378
3	3.2921764254435466	3.2593028496087424
4	1.8610897927858787	2.456674848133843
5	1.3159676764028712	2.1713787696513505
6	0.9107940950747144	1.803398971295341
7	0.6557266037650671	1.514750850115273
8	0.5360931786375334	1.4153069167538819
9	0.4029163468917882	1.196679243034269
>10	5.218726016884114	39.05837396133233
>50	0.21895174032775044	4.467155568301594
>100	0.04514468872737122	2.9538945149407994
>500	0.004514468872737122	1.0104546487245478
>1k	0.004514468872737122	3.7706755855832363
>5k	0.002257234436368561	3.8690021713787695
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC	5282	1.9672766142878957	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG	5106	1.9017255570908738	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC	4269	1.5899855862164005	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3403	1.2674445888719632	No Hit
GCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	1434	0.5340921364802808	TruSeq Adapter, Index 22 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCT	1018	0.3791532740145926	No Hit
GAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT	802	0.298704249272793	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTC	770	0.2867858752369708	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTA	628	0.23389809045300994	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGC	513	0.19106643376177404	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCG	490	0.18250010242352688	No Hit
GAATCTATCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTC	436	0.16238784623807698	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	433	0.16127049867221863	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	371	0.1381786489778132	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	310	0.11545924847202721	No Hit
AGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAAGGAAGG	298	0.11098985820859389	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	283	0.10540312037930226	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTT	281	0.10465822200206337	No Hit
CCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC	273	0.10167862849310783	TruSeq Adapter, Index 22 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11508679928340776	0.0
2	0.0	0.0	0.0	0.9910872909163367	0.0
3	0.0	0.0	0.0	1.2119496597676662	0.0
4	0.0	0.0	0.0	1.8171795912742603	0.0
5	3.724491886194426E-4	0.0	0.0	4.255604429165751	0.0
6	3.724491886194426E-4	0.0	0.0	4.801242490493235	0.0
7	3.724491886194426E-4	0.0	0.0	5.591207219555073	0.0
8	3.724491886194426E-4	0.0	0.0	6.60166186827962	0.0
9	3.724491886194426E-4	0.0	0.0	6.8731773267831935	0.0
10	3.724491886194426E-4	0.0	0.0	9.671760530069685	0.0
11	3.724491886194426E-4	0.0	0.0	10.533235503346456	0.0
12	7.448983772388852E-4	0.0	0.0	13.500910638266175	0.0
13	7.448983772388852E-4	0.0	0.0	13.920288424651666	0.0
14	7.448983772388852E-4	0.0	0.0	14.10576812058415	0.0
15	7.448983772388852E-4	0.0	0.0	14.638742909498571	0.0
16	7.448983772388852E-4	0.0	0.0	15.185870767580532	0.0
17	7.448983772388852E-4	0.0	0.0	15.74230985537798	0.0
18	7.448983772388852E-4	0.0	0.0	16.405641860309206	0.0
19	7.448983772388852E-4	0.0	0.0	16.85109108989806	0.0
20	7.448983772388852E-4	0.0	0.0	17.215346396367874	0.0
21	7.448983772388852E-4	0.0	0.0	17.642545615714376	0.0
22	7.448983772388852E-4	0.0	0.0	18.085015251794275	0.0
23	7.448983772388852E-4	0.0	0.0	18.529347133817268	0.0
24	7.448983772388852E-4	0.0	0.0	18.91036265377496	0.0
25	7.448983772388852E-4	0.0	0.0	19.227316913290103	0.0
26	7.448983772388852E-4	0.0	0.0	19.526766060940137	0.0
27	7.448983772388852E-4	0.0	0.0	19.856011143679723	0.0
28	7.448983772388852E-4	0.0	0.0	20.18674602317379	0.0
29	7.448983772388852E-4	0.0	0.0	20.501465587557217	0.0
30	7.448983772388852E-4	0.0	0.0	20.85491986755707	0.0
31	7.448983772388852E-4	0.0	0.0	21.197573121086954	0.0
32	7.448983772388852E-4	0.0	0.0	21.55512434216162	0.0
33	7.448983772388852E-4	0.0	0.0	21.900012290823224	0.0
34	7.448983772388852E-4	0.0	0.0	22.25756351189789	0.0
35	7.448983772388852E-4	0.0	0.0	22.61809432648151	0.0
36	7.448983772388852E-4	0.0	0.0	22.974528199990317	0.0
37	7.448983772388852E-4	0.0	0.0	23.309732469747814	0.0
38	7.448983772388852E-4	0.0	0.0	23.66132450380457	0.0
39	7.448983772388852E-4	0.0	0.0	24.02222776757681	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	25	3.882793E-5	45.000004	13
CGGGTTG	25	3.882793E-5	45.000004	6
AGCGTGA	25	3.882793E-5	45.000004	28
TCGTTTC	25	3.882793E-5	45.000004	43
ACTATGC	55	1.8189894E-12	45.000004	8
GGCACCG	25	3.882793E-5	45.000004	8
TAGCGAG	25	3.882793E-5	45.000004	1
GCACGTC	25	3.882793E-5	45.000004	39
TACATGC	25	3.882793E-5	45.000004	15
ACGGCCT	25	3.882793E-5	45.000004	8
TCTGTCG	25	3.882793E-5	45.000004	24
CTCGATA	25	3.882793E-5	45.000004	1
GCGGAGT	25	3.882793E-5	45.000004	9
CACGTCA	25	3.882793E-5	45.000004	40
CACAACG	25	3.882793E-5	45.000004	12
TAGTGAG	25	3.882793E-5	45.000004	1
TCGACAT	25	3.882793E-5	45.000004	13
CGTTTCG	25	3.882793E-5	45.000004	44
GACCTAT	25	3.882793E-5	45.000004	33
CGATAGC	25	3.882793E-5	45.000004	10
>>END_MODULE