##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936684.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	377673
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13944867650057	31.0	31.0	33.0	28.0	34.0
2	31.197056713082482	31.0	31.0	34.0	28.0	34.0
3	30.989821882951652	31.0	31.0	34.0	28.0	34.0
4	34.973469112168466	35.0	35.0	37.0	32.0	37.0
5	35.202677978039205	35.0	35.0	37.0	33.0	37.0
6	35.18101902968971	36.0	35.0	37.0	32.0	37.0
7	30.91284788692864	35.0	35.0	37.0	0.0	37.0
8	32.978253674475006	35.0	35.0	37.0	17.0	37.0
9	36.22151967442735	39.0	35.0	39.0	32.0	39.0
10	36.127930776094665	37.0	35.0	39.0	32.0	39.0
11	36.39658381721754	38.0	35.0	39.0	32.0	39.0
12	36.37892038880195	38.0	35.0	39.0	32.0	39.0
13	36.22835892425458	38.0	35.0	39.0	32.0	39.0
14	37.20129847778369	39.0	36.0	40.0	32.0	41.0
15	37.366425452706444	39.0	36.0	41.0	32.0	41.0
16	37.382632594863814	39.0	36.0	40.0	32.0	41.0
17	37.287134637636264	39.0	36.0	40.0	32.0	41.0
18	37.206652315627544	39.0	36.0	40.0	32.0	41.0
19	36.98008859516036	39.0	35.0	40.0	32.0	41.0
20	36.967953758939615	39.0	35.0	40.0	32.0	41.0
21	36.95125147945445	39.0	35.0	40.0	31.0	41.0
22	37.04647936177593	39.0	35.0	40.0	32.0	41.0
23	37.13484416413141	39.0	35.0	40.0	32.0	41.0
24	37.071990849226715	39.0	35.0	40.0	32.0	41.0
25	36.794684819936826	38.0	35.0	40.0	31.0	41.0
26	36.73886933934912	39.0	35.0	40.0	31.0	41.0
27	36.77032512252663	39.0	35.0	40.0	31.0	41.0
28	36.7406009960998	39.0	35.0	40.0	31.0	41.0
29	36.71631278910592	39.0	35.0	40.0	31.0	41.0
30	36.51660298724028	38.0	35.0	40.0	31.0	41.0
31	36.52179530969913	39.0	35.0	40.0	31.0	41.0
32	36.31175911436613	38.0	35.0	40.0	30.0	41.0
33	36.14419881749556	38.0	35.0	40.0	30.0	41.0
34	35.995204846520664	38.0	35.0	40.0	29.0	41.0
35	35.91025040180262	38.0	35.0	40.0	29.0	41.0
36	35.82807613994117	38.0	35.0	40.0	29.0	41.0
37	35.77573191623441	38.0	35.0	40.0	29.0	41.0
38	35.60719723146743	38.0	35.0	40.0	28.0	41.0
39	35.54216213496861	38.0	35.0	40.0	27.0	41.0
40	35.35791809316525	38.0	34.0	40.0	26.0	41.0
41	35.166029872402845	38.0	34.0	40.0	26.0	41.0
42	35.16613313633752	38.0	34.0	40.0	26.0	41.0
43	35.07018770205972	38.0	34.0	40.0	25.0	41.0
44	34.88634877261546	38.0	34.0	40.0	24.0	41.0
45	34.824088563386844	37.0	34.0	40.0	24.0	41.0
46	34.67314581661914	37.0	34.0	40.0	24.0	41.0
47	34.602971353525405	37.0	34.0	40.0	24.0	41.0
48	34.520622337312965	37.0	34.0	40.0	24.0	41.0
49	34.41572736203012	37.0	33.0	40.0	24.0	41.0
50	34.26077056077612	37.0	33.0	40.0	23.0	41.0
51	32.44429969841635	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	26.0
10	42.0
11	33.0
12	25.0
13	17.0
14	22.0
15	29.0
16	47.0
17	137.0
18	218.0
19	373.0
20	634.0
21	975.0
22	1538.0
23	2054.0
24	2988.0
25	4054.0
26	5323.0
27	5927.0
28	6726.0
29	7501.0
30	9152.0
31	11436.0
32	15175.0
33	20779.0
34	31994.0
35	34297.0
36	37799.0
37	54454.0
38	70024.0
39	53857.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.1277348393981	19.10753482510002	20.879173253052244	13.885557082449631
2	31.995668210330102	25.944931197093783	23.47003889608206	18.58936169649406
3	32.01949834910094	25.397102784684105	26.718881148506778	15.864517717708177
4	27.34667291545861	24.208508418658468	28.750532868380844	19.694285797502072
5	26.309797099607334	28.969240586433237	25.39816190196281	19.32280041199662
6	25.039385923801806	34.49439065011266	26.63018007641531	13.836043349670218
7	66.70029364026551	17.56890219846269	11.67782711499101	4.052977046280777
8	75.75495203522624	7.797750964458672	11.096106949662804	5.351190050652284
9	70.96562370092646	6.976140735503994	13.299865227326285	8.758370336243258
10	40.97168714734704	25.589862129408246	19.380257524366318	14.058193198878394
11	32.6801227516926	24.807704019085293	23.900305290555586	18.611867938666517
12	27.778527985850193	23.483013082746183	28.738882578315106	19.999576353088518
13	24.54001212689284	26.123392458555415	31.8079396726798	17.528655741871937
14	20.657552962483418	29.635954913377443	29.5128325297281	20.193659594411038
15	18.472329237197258	24.90832016056218	36.983316255067216	19.636034347173347
16	21.12594757899029	26.239630579893188	31.892669054976132	20.741752786140392
17	22.18665353361241	24.404974673858074	29.736571054854334	23.671800737675184
18	24.982987928710816	23.792275328127772	30.32676415841217	20.89797258474924
19	24.112393525616074	26.769453998564895	27.39989355871349	21.718258917105537
20	27.026025159330956	25.218376743902795	28.755828454774367	18.999769641991882
21	24.525714043630337	27.075274112790694	29.23216645087152	19.166845392707447
22	23.83013877084144	23.046127205280758	29.89861599849605	23.225118025381747
23	21.8776560675505	27.244203318743992	28.82361196061143	22.05452865309408
24	22.35293494636895	23.591307824493676	30.51502225470182	23.540734974435555
25	22.235637707752474	27.921508818475242	28.02530231178824	21.817551161984046
26	21.510407151159868	25.26127099369031	29.542223034212135	23.68609882093769
27	21.97191750535516	27.01993523497841	29.47496908701443	21.533178172652004
28	18.52607943909149	26.55445319098797	31.068940591464045	23.850526778456498
29	20.642195761942208	24.585554169877117	31.39011790623105	23.382132161949624
30	20.014933553629728	24.77487138344547	32.66635422706945	22.543840835855356
31	23.241799122521336	24.027399364000075	28.05839972674774	24.67240178673085
32	23.37048187188388	26.033897048504922	28.665803486084524	21.929817593526675
33	22.680731744127858	25.1222618508604	26.001064412865098	26.195941992146643
34	20.639018410106097	24.816176957314926	30.52826122068562	24.016543411893355
35	19.36542988246446	26.366724653337677	30.247330362509366	24.020515101688495
36	22.029639397044534	26.534065183372917	28.515673611828223	22.920621807754326
37	20.78094012545244	26.99954722736335	28.678248114109294	23.54126453307491
38	20.070272431442014	28.507730232237943	25.628519910080943	25.793477426239104
39	21.463541211577212	25.081221056310614	27.21799016609607	26.2372475660161
40	23.369422754605175	22.412510293296055	30.548914007620347	23.669152944478427
41	19.74671210279792	25.531345899759845	28.58954704201783	26.132394955424402
42	21.706343847720117	24.052023840729944	31.28129360584421	22.96033870570573
43	22.73898319445658	25.275569076952813	27.48250470645241	24.502943022138197
44	21.322413834189895	24.477788986768978	28.986716021531855	25.213081157509276
45	21.333005006976936	23.868002213555112	27.339788653147036	27.45920412632092
46	22.61612559012691	25.156947941737958	27.661230747233716	24.565695720901417
47	19.62835574690274	25.662941221638825	31.38032107140304	23.328381960055392
48	21.509083254561485	25.350501624421128	28.539768529918742	24.60064659109865
49	21.842705197353265	23.376836575556105	31.45392972227297	23.32652850481766
50	20.7703489526654	25.01264321251453	29.651047334598978	24.56596050022109
51	20.30460212935529	24.53206874730256	27.925215728950707	27.238113394391444
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	135.0
1	390.0
2	645.0
3	2020.0
4	3395.0
5	2457.0
6	1519.0
7	1424.5
8	1330.0
9	1318.0
10	1306.0
11	1300.5
12	1295.0
13	1297.5
14	1300.0
15	1259.0
16	1218.0
17	1308.5
18	1399.0
19	1270.5
20	1142.0
21	1262.0
22	1382.0
23	1556.5
24	1731.0
25	1871.5
26	2505.5
27	2999.0
28	3502.0
29	4005.0
30	4505.5
31	5006.0
32	5897.0
33	6788.0
34	8094.5
35	9401.0
36	10467.5
37	11534.0
38	12612.5
39	13691.0
40	15675.0
41	17659.0
42	19740.0
43	21821.0
44	24007.5
45	26194.0
46	32915.5
47	39637.0
48	36708.5
49	33780.0
50	33779.5
51	33779.0
52	30218.5
53	26658.0
54	24303.5
55	21949.0
56	19756.5
57	17564.0
58	16507.0
59	15450.0
60	14455.5
61	13461.0
62	12402.0
63	11343.0
64	9857.0
65	8371.0
66	7111.0
67	5851.0
68	5100.0
69	4349.0
70	3354.5
71	2360.0
72	2067.0
73	1774.0
74	1431.0
75	905.0
76	722.0
77	496.0
78	270.0
79	218.5
80	167.0
81	125.0
82	83.0
83	73.0
84	63.0
85	39.5
86	16.0
87	12.0
88	8.0
89	8.0
90	8.0
91	6.5
92	5.0
93	5.5
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	377673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.92756258052904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.11174440264264	25.483584087533607
2	8.852215593062335	6.006681970199148
3	3.5948916324700297	3.6589773249254143
4	2.0626788763555735	2.7992666664435615
5	1.3937109056980064	2.364260698611746
6	1.0014366102708672	2.038578195923863
7	0.7252843082664752	1.7224990130170588
8	0.5787640774392573	1.570884356534605
9	0.4860538029379379	1.4841558735012916
>10	6.021077774450316	44.807980840560354
>50	0.13553262830202467	2.8673255892737175
>100	0.030378002895281395	1.9056483708988734
>500	0.0023367694534831844	0.5134759096736252
>1k	0.0038946157558053065	2.776681102903129
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC	2671	0.7072255628546388	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG	2568	0.6799532929280092	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	2131	0.5642447302295901	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1748	0.462834250793676	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	1389	0.36777847502998623	TruSeq Adapter, Index 13 (95% over 21bp)
ATGAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC	754	0.1996436070357161	No Hit
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	638	0.16892920595329822	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	551	0.14589340514148483	No Hit
GAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	417	0.11041297630489867	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	408	0.10802996242781453	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11941547317388322	0.0
2	0.0	0.0	0.0	0.9463212885220813	0.0
3	0.0	0.0	0.0	1.1769440759598913	0.0
4	0.0	0.0	0.0	1.618066422540134	0.0
5	2.64779319676016E-4	0.0	0.0	3.0497282040283524	0.0
6	2.64779319676016E-4	0.0	0.0	3.665604901594766	0.0
7	2.64779319676016E-4	0.0	0.0	4.388982002949642	0.0
8	2.64779319676016E-4	0.0	0.0	5.385081803570814	0.0
9	2.64779319676016E-4	0.0	0.0	5.692755373034345	0.0
10	2.64779319676016E-4	0.0	0.0	7.140303913703124	0.0
11	2.64779319676016E-4	0.0	0.0	8.02784419325713	0.0
12	2.64779319676016E-4	0.0	0.0	9.821988863381815	0.0
13	2.64779319676016E-4	0.0	0.0	10.197710718002082	0.0
14	2.64779319676016E-4	0.0	0.0	10.452428423530408	0.0
15	2.64779319676016E-4	0.0	0.0	10.827620719511323	0.0
16	2.64779319676016E-4	0.0	0.0	11.381274276953873	0.0
17	2.64779319676016E-4	0.0	0.0	12.001387443635103	0.0
18	2.64779319676016E-4	0.0	0.0	12.694844481866589	0.0
19	2.64779319676016E-4	0.0	0.0	13.076126702200051	0.0
20	2.64779319676016E-4	0.0	0.0	13.43649135627911	0.0
21	2.64779319676016E-4	0.0	0.0	13.903562076187601	0.0
22	2.64779319676016E-4	0.0	0.0	14.34494920208752	0.0
23	2.64779319676016E-4	0.0	0.0	14.794014928258044	0.0
24	2.64779319676016E-4	0.0	0.0	15.1930373630098	0.0
25	2.64779319676016E-4	0.0	0.0	15.503623504989767	0.0
26	2.64779319676016E-4	0.0	0.0	15.832744199347054	0.0
27	2.64779319676016E-4	0.0	0.0	16.179340328802958	0.0
28	2.64779319676016E-4	0.0	0.0	16.511903154316034	0.0
29	2.64779319676016E-4	0.0	0.0	16.8677665599606	0.0
30	2.64779319676016E-4	0.0	0.0	17.31497883089339	0.0
31	2.64779319676016E-4	0.0	0.0	17.681698188644674	0.0
32	2.64779319676016E-4	0.0	0.0	18.019821379870947	0.0
33	2.64779319676016E-4	0.0	0.0	18.369594861162962	0.0
34	2.64779319676016E-4	0.0	0.0	18.731018632520726	0.0
35	2.64779319676016E-4	0.0	0.0	19.146722164412072	0.0
36	2.64779319676016E-4	0.0	0.0	19.483521459039963	0.0
37	2.64779319676016E-4	0.0	0.0	19.855536403184765	0.0
38	2.64779319676016E-4	0.0	0.0	20.22331487821475	0.0
39	2.64779319676016E-4	0.0	0.0	20.761876014435767	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACC	20	7.0272654E-4	45.0	14
GGCGTAC	20	7.0272654E-4	45.0	8
TTACTCC	25	3.8858154E-5	45.0	32
ATACTCG	20	7.0272654E-4	45.0	44
TAGCACG	25	3.8858154E-5	45.0	1
TCGTCAG	20	7.0272654E-4	45.0	1
TACTCGC	20	7.0272654E-4	45.0	45
TGCGTTG	25	3.8858154E-5	45.0	1
CCCGACG	20	7.0272654E-4	45.0	10
CTAAACG	30	2.1616179E-6	44.999996	45
CGACAGG	60	3.6379788E-12	41.249996	2
AATGCGG	55	6.002665E-11	40.909092	2
CGAATAT	55	6.002665E-11	40.909092	14
ACAACGA	215	0.0	40.813953	13
CGTTTTT	1120	0.0	40.78125	1
CGTACGG	50	1.0768417E-9	40.5	31
TGGGCGA	150	0.0	40.5	6
ATGGGCG	80	0.0	39.375	5
TACGGGT	40	3.4500044E-7	39.375	4
GGGCGAT	455	0.0	39.065937	7
>>END_MODULE