##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936682.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448291
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.243464624540756	31.0	31.0	33.0	30.0	34.0
2	31.273438458501285	31.0	31.0	34.0	28.0	34.0
3	31.086060170737312	31.0	31.0	34.0	28.0	34.0
4	35.056249177431624	35.0	35.0	37.0	32.0	37.0
5	35.25489246940046	35.0	35.0	37.0	33.0	37.0
6	35.2229489327245	37.0	35.0	37.0	33.0	37.0
7	30.908414400467553	35.0	35.0	37.0	0.0	37.0
8	33.049661938339156	36.0	35.0	37.0	17.0	37.0
9	36.42731395455184	39.0	35.0	39.0	32.0	39.0
10	36.17071500431639	37.0	35.0	39.0	32.0	39.0
11	36.38759644962759	38.0	35.0	39.0	32.0	39.0
12	36.402722784976724	38.0	35.0	39.0	32.0	39.0
13	36.29518772404532	38.0	35.0	39.0	32.0	39.0
14	37.21673198881976	39.0	36.0	40.0	32.0	41.0
15	37.48227156021424	39.0	36.0	41.0	32.0	41.0
16	37.4343517938125	39.0	36.0	41.0	32.0	41.0
17	37.39043389227087	39.0	36.0	41.0	32.0	41.0
18	37.34811985964474	39.0	36.0	40.0	32.0	41.0
19	37.18739613331519	39.0	35.0	40.0	32.0	41.0
20	37.1471811836508	39.0	35.0	40.0	32.0	41.0
21	37.134872214699826	39.0	35.0	40.0	32.0	41.0
22	37.24439705459177	39.0	35.0	40.0	32.0	41.0
23	37.303534980626424	39.0	35.0	40.0	32.0	41.0
24	37.26352525480101	39.0	35.0	41.0	32.0	41.0
25	36.894006348554846	39.0	35.0	40.0	31.0	41.0
26	36.904238541483096	39.0	35.0	40.0	31.0	41.0
27	36.92150634297811	39.0	35.0	40.0	31.0	41.0
28	36.89803275104787	39.0	35.0	40.0	31.0	41.0
29	36.83985848477886	39.0	35.0	40.0	31.0	41.0
30	36.60994309499856	39.0	35.0	40.0	31.0	41.0
31	36.591446181163576	39.0	35.0	40.0	31.0	41.0
32	36.469833211016955	39.0	35.0	40.0	30.0	41.0
33	36.359099335030145	39.0	35.0	40.0	30.0	41.0
34	36.256728330481764	38.0	35.0	40.0	30.0	41.0
35	36.160453812367415	38.0	35.0	40.0	30.0	41.0
36	36.07391404244118	38.0	35.0	40.0	30.0	41.0
37	35.99104376398366	38.0	35.0	40.0	29.0	41.0
38	35.810565012458426	38.0	35.0	40.0	29.0	41.0
39	35.72927629597739	38.0	35.0	40.0	29.0	41.0
40	35.593369039307056	38.0	35.0	40.0	28.0	41.0
41	35.37796654405286	38.0	34.0	40.0	27.0	41.0
42	35.371080391977536	38.0	34.0	40.0	27.0	41.0
43	35.34765141392533	38.0	34.0	40.0	27.0	41.0
44	35.14580038412549	38.0	34.0	40.0	26.0	41.0
45	35.06824584923632	38.0	34.0	40.0	26.0	41.0
46	35.008440945724985	37.0	34.0	40.0	26.0	41.0
47	34.95142664028499	37.0	34.0	40.0	26.0	41.0
48	34.83229197106344	37.0	34.0	40.0	26.0	41.0
49	34.73283871413881	37.0	34.0	40.0	26.0	41.0
50	34.58563299285509	37.0	34.0	40.0	25.0	41.0
51	32.89435433680355	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	24.0
9	45.0
10	64.0
11	50.0
12	33.0
13	27.0
14	34.0
15	45.0
16	89.0
17	131.0
18	256.0
19	424.0
20	655.0
21	1092.0
22	1563.0
23	2229.0
24	3052.0
25	4175.0
26	5257.0
27	6301.0
28	7246.0
29	8580.0
30	10517.0
31	13130.0
32	17320.0
33	24668.0
34	36942.0
35	38017.0
36	45974.0
37	67446.0
38	85751.0
39	67151.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.532863697910514	21.2830505185248	23.413363194889033	12.770722588675659
2	32.60716811178453	24.25433479592497	26.13235599197842	17.006141100312075
3	34.12961669986683	22.885357948296978	26.7897414848837	16.195283866952494
4	31.257821370493723	25.110921254274565	24.012973715733754	19.61828365949796
5	25.653426011229314	30.450756316767457	24.617937901943158	19.27787977006007
6	27.75964719345247	34.28175002397996	24.17179912155274	13.786803661014831
7	70.88899844074497	17.963777992420102	7.4480638692278	3.699159697607134
8	83.3391703157101	5.212016301911036	7.568298270543019	3.8805151118358387
9	77.08006629622277	7.187741890869994	9.933056876002418	5.799134936904823
10	45.704910426486364	22.697310452362416	16.968442373369083	14.629336747782132
11	33.413340887950014	25.33376757507958	22.444572833271245	18.808318703699157
12	30.4346953206734	21.52307318237484	27.923157056465552	20.1190744404862
13	26.849747150846216	23.051321574602213	30.088714696480633	20.010216578070942
14	21.986834444590677	25.982453361767245	30.821943782052287	21.208768411589794
15	20.815720146065836	25.301868652281666	33.42226366355782	20.460147538094674
16	25.101552339886368	24.10309374937262	30.01867090795934	20.776683002781677
17	26.824094170973765	23.658739524103765	26.67196084686063	22.84520545806184
18	27.012810875078912	24.204813391301634	28.00145441242407	20.780921321195382
19	26.860454481575584	25.6494107622058	26.248798213660322	21.241336542558294
20	26.796210497199365	25.733507922309396	26.803125648295417	20.667155932195826
21	27.162936574680284	24.949419015773238	28.66820881971755	19.21943558982893
22	25.938731761288985	22.190942936619294	29.545317661965107	22.325007640126614
23	24.621730081576477	24.281995400309174	29.369985121271675	21.726289396842677
24	25.011432306247507	25.42411067810864	27.589222179343327	21.97523483630053
25	25.56263676941986	24.684635649611526	26.806025550367952	22.94670203060066
26	22.637750925180296	25.52694566698863	26.754719590623054	25.08058381720802
27	22.36850617121468	25.9797765290849	28.79379688639745	22.857920413302963
28	21.89872203546357	26.101126277351096	28.946822488071366	23.053329199113968
29	23.16865607384489	26.65299994869404	27.843521284165867	22.3348226932952
30	25.054930837335572	25.20996406352124	27.935648942316487	21.799456156826704
31	26.196153837574254	25.314360538132597	26.727505125019242	21.761980499273907
32	26.49484375104564	26.00832941102989	25.81091300070713	21.685913837217345
33	26.981358091061384	24.610130473286326	25.824743302899233	22.583768132753054
34	24.32170175176392	25.398011559455803	28.074398103017906	22.205888585762374
35	24.134769602780338	26.55351100066698	27.110292198594216	22.201427197958466
36	27.02418741397887	26.406954411308725	26.048035762484634	20.520822412227773
37	25.03820063307093	26.959943429602646	26.39022420704408	21.611631730282337
38	24.760033103497506	26.326426361448256	26.229168107323147	22.684372427731095
39	24.71876526631139	24.362300380779452	27.028648801782772	23.89028555112639
40	25.776783384007263	24.57778541170802	27.53613166447687	22.109299539807846
41	22.964324512426078	24.843237986040318	28.579873341200248	23.612564160333356
42	23.2705987851641	25.177172863162546	27.88791209281471	23.66431625885864
43	23.272383340285664	24.601653836458905	28.31754373833068	23.808419084924747
44	23.770720357981755	24.301848576036548	27.982716583647676	23.94471448233402
45	23.60631821740789	24.9418346565066	26.90841440046755	24.543432725617958
46	24.08814810022954	25.49660822992208	27.55420028508268	22.8610433847657
47	23.29759018137772	25.27599260301902	29.137100677907878	22.28931653769538
48	22.570383969341343	26.14462480843916	28.597718892415863	22.687272329803633
49	23.855486726255936	24.49168062709267	29.061926293412093	22.5909063532393
50	22.280393762087574	24.507295484406335	29.432890689306724	23.779420064199368
51	22.546961683370846	24.868890965912765	28.29144461967784	24.292702731038542
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	224.0
1	581.5
2	939.0
3	1639.5
4	2340.0
5	1713.5
6	1087.0
7	1037.5
8	988.0
9	1007.0
10	1026.0
11	992.0
12	958.0
13	1032.5
14	1107.0
15	1109.5
16	1112.0
17	1102.5
18	1093.0
19	1071.0
20	1049.0
21	1113.0
22	1177.0
23	1527.0
24	1877.0
25	2198.0
26	2884.0
27	3249.0
28	3944.0
29	4639.0
30	5652.0
31	6665.0
32	7912.0
33	9159.0
34	10893.5
35	12628.0
36	13445.0
37	14262.0
38	14741.5
39	15221.0
40	16329.0
41	17437.0
42	19936.0
43	22435.0
44	24371.0
45	26307.0
46	29537.5
47	32768.0
48	34284.0
49	35800.0
50	35174.5
51	34549.0
52	31847.0
53	29145.0
54	28347.5
55	27550.0
56	27202.5
57	26855.0
58	26309.0
59	25763.0
60	23510.0
61	21257.0
62	20003.5
63	18750.0
64	16834.0
65	14918.0
66	12903.5
67	10889.0
68	9544.5
69	8200.0
70	6709.0
71	5218.0
72	4302.5
73	3387.0
74	2647.0
75	1391.5
76	876.0
77	663.5
78	451.0
79	355.0
80	259.0
81	185.5
82	112.0
83	91.5
84	71.0
85	46.0
86	21.0
87	16.0
88	11.0
89	14.5
90	18.0
91	14.5
92	11.0
93	7.0
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	448291.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.635640074236306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7264015506984	24.461990664536728
2	8.62627249563492	5.803003936909206
3	3.9191465488476225	3.9546900814567207
4	2.263500411509021	3.045371405976128
5	1.5274869333795678	2.568900035462705
6	1.1607443565123903	2.342542763631107
7	0.8736050835624648	2.056898631041114
8	0.7330474009144125	1.9725214827609263
9	0.6167018825124798	1.866884629796436
>10	7.467783913393459	48.68584468891841
>50	0.05753426204700076	1.1927070893175578
>100	0.02645253427448311	1.6825133203278644
>500	6.613133568620777E-4	0.12300765020376904
>1k	6.613133568620777E-4	0.24312361966133733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1093	0.24381484348336238	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	553	0.12335737277795004	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.059113388401730124	0.0
2	0.0	0.0	0.0	0.3301426974889079	0.0
3	0.0	0.0	0.0	0.4488156130727585	0.0
4	0.0	0.0	0.0	0.6228097374250208	0.0
5	0.0	0.0	0.0	1.1546071636503967	0.0
6	0.0	0.0	0.0	1.5235639350332708	0.0
7	0.0	0.0	0.0	1.8053005748498185	0.0
8	0.0	0.0	0.0	2.2610313390186287	0.0
9	0.0	0.0	0.0	2.441717545076747	0.0
10	0.0	0.0	0.0	2.9248858442395678	0.0
11	0.0	0.0	0.0	3.515127450696088	0.0
12	0.0	0.0	0.0	4.121206983856468	0.0
13	0.0	0.0	0.0	4.418335411596486	0.0
14	0.0	0.0	0.0	4.569799527539032	0.0
15	0.0	0.0	0.0	4.74490899884227	0.0
16	0.0	0.0	0.0	5.0786208065743015	0.0
17	0.0	0.0	0.0	5.472338280268843	0.0
18	0.0	0.0	0.0	5.912231117733794	0.0
19	0.0	0.0	0.0	6.194636965720926	0.0
20	0.0	0.0	0.0	6.477935091268841	0.0
21	0.0	0.0	0.0	6.842207405457616	0.0
22	0.0	0.0	0.0	7.186849613309212	0.0
23	0.0	0.0	0.0	7.5076233964099215	0.0
24	0.0	0.0	0.0	7.778875774887294	0.0
25	0.0	0.0	0.0	8.02670586739417	0.0
26	0.0	0.0	0.0	8.263159421001092	0.0
27	0.0	0.0	0.0	8.533519521917682	0.0
28	0.0	0.0	0.0	8.796964471738224	0.0
29	0.0	0.0	0.0	9.069109127776377	0.0
30	0.0	0.0	0.0	9.404382421239776	0.0
31	0.0	0.0	0.0	9.679873118130857	0.0
32	0.0	0.0	0.0	9.960717480386624	0.0
33	0.0	0.0	0.0	10.247807785567856	0.0
34	0.0	0.0	0.0	10.560774140011734	0.0
35	0.0	0.0	0.0	10.892032184451617	0.0
36	0.0	0.0	0.0	11.179568628413241	0.0
37	0.0	0.0	0.0	11.489635080784579	0.0
38	0.0	0.0	0.0	11.862160962410577	0.0
39	0.0	0.0	0.0	12.47649406300818	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGC	20	7.0286775E-4	45.0	18
TATAGCG	20	7.0286775E-4	45.0	1
CGATGCA	20	7.0286775E-4	45.0	10
ATAACGG	20	7.0286775E-4	45.0	2
ACGGGTA	30	2.1625274E-6	44.999996	5
CGAATAT	115	0.0	43.04348	14
TACGGGA	60	3.6379788E-12	41.249996	4
TACGAAT	120	0.0	41.249996	12
CGTTTTT	480	0.0	40.781246	1
GTCGTTT	40	3.4519326E-7	39.375	9
TATTGGG	145	0.0	38.793102	3
CGCGGTA	35	6.2397667E-6	38.571426	30
TAACGCC	35	6.2397667E-6	38.571426	12
ATCACGG	35	6.2397667E-6	38.571426	2
ATAACGC	35	6.2397667E-6	38.571426	11
TTTAGCG	30	1.1388113E-4	37.499996	1
GCGCGAC	60	1.546141E-10	37.499996	9
CGATTGA	30	1.1388113E-4	37.499996	10
GTACGAG	30	1.1388113E-4	37.499996	1
AAGCTAC	145	0.0	37.24138	8
>>END_MODULE