##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936681.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1032097
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17777592609997	31.0	31.0	33.0	28.0	34.0
2	31.204973951091805	31.0	31.0	34.0	28.0	34.0
3	31.037341451433345	31.0	31.0	34.0	28.0	34.0
4	35.000730551488864	35.0	35.0	37.0	32.0	37.0
5	35.207451431406156	35.0	35.0	37.0	33.0	37.0
6	35.171369551505336	36.0	35.0	37.0	32.0	37.0
7	31.040701600721636	35.0	35.0	37.0	0.0	37.0
8	33.06300376805668	36.0	35.0	37.0	17.0	37.0
9	36.323747671003794	39.0	35.0	39.0	32.0	39.0
10	36.09915056433649	37.0	35.0	39.0	32.0	39.0
11	36.37221404577283	38.0	35.0	39.0	32.0	39.0
12	36.46428678699773	38.0	35.0	39.0	32.0	39.0
13	36.45785134536773	39.0	35.0	39.0	32.0	39.0
14	37.489370669617294	39.0	36.0	41.0	32.0	41.0
15	37.65972093708246	39.0	36.0	41.0	33.0	41.0
16	37.58049679439045	39.0	36.0	41.0	33.0	41.0
17	37.48059630054152	39.0	36.0	41.0	32.0	41.0
18	37.38479232087682	39.0	36.0	40.0	32.0	41.0
19	37.21125243073083	39.0	36.0	40.0	32.0	41.0
20	37.20452438094481	39.0	35.0	40.0	32.0	41.0
21	37.21642054961888	39.0	35.0	40.0	32.0	41.0
22	37.32255204694908	39.0	35.0	40.0	32.0	41.0
23	37.39135275075889	39.0	35.0	40.0	32.0	41.0
24	37.32223230956005	39.0	35.0	41.0	32.0	41.0
25	37.00615639809049	39.0	35.0	40.0	31.0	41.0
26	36.99504213266776	39.0	35.0	40.0	31.0	41.0
27	36.989958308182274	39.0	35.0	40.0	31.0	41.0
28	36.922135225661926	39.0	35.0	40.0	31.0	41.0
29	36.88315826903867	39.0	35.0	40.0	31.0	41.0
30	36.649627893502256	39.0	35.0	40.0	31.0	41.0
31	36.671539593662224	39.0	35.0	40.0	31.0	41.0
32	36.551420070012796	39.0	35.0	40.0	30.0	41.0
33	36.43674189538386	39.0	35.0	40.0	30.0	41.0
34	36.36909612177925	39.0	35.0	40.0	30.0	41.0
35	36.312030749047814	39.0	35.0	40.0	30.0	41.0
36	36.27914236743252	39.0	35.0	40.0	30.0	41.0
37	36.207270246885706	39.0	35.0	40.0	30.0	41.0
38	36.06735994775685	39.0	35.0	40.0	29.0	41.0
39	35.996915018646504	38.0	35.0	40.0	29.0	41.0
40	35.88960921308753	38.0	35.0	40.0	29.0	41.0
41	35.721929237271304	38.0	35.0	40.0	28.0	41.0
42	35.72880359113533	38.0	35.0	40.0	28.0	41.0
43	35.68440078791044	38.0	35.0	40.0	28.0	41.0
44	35.51749690193848	38.0	34.0	40.0	27.0	41.0
45	35.44222006264915	38.0	34.0	40.0	27.0	41.0
46	35.314367738691224	38.0	34.0	40.0	27.0	41.0
47	35.28348110691146	38.0	34.0	40.0	27.0	41.0
48	35.19872938299404	38.0	34.0	40.0	27.0	41.0
49	35.06756729260913	38.0	34.0	40.0	26.0	41.0
50	34.90780033272066	37.0	34.0	40.0	26.0	41.0
51	33.23472599959112	36.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	51.0
9	91.0
10	135.0
11	99.0
12	70.0
13	47.0
14	58.0
15	96.0
16	155.0
17	275.0
18	533.0
19	1011.0
20	1597.0
21	2567.0
22	3756.0
23	5280.0
24	7048.0
25	9369.0
26	11654.0
27	13627.0
28	15649.0
29	18647.0
30	22761.0
31	29305.0
32	37663.0
33	52220.0
34	78563.0
35	85855.0
36	103834.0
37	154902.0
38	206647.0
39	168515.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.486128726272824	19.90510581854225	22.353615987644574	13.255149467540356
2	32.52785348663934	24.939225673555875	24.72839277703549	17.804528062769297
3	33.62232425828193	23.05471288066916	26.245885803369255	17.077077057679656
4	29.733833157154805	25.285995405470608	25.645748413182094	19.334423024192493
5	25.827611164454506	30.19541767876469	24.295293950084147	19.68167720669666
6	26.640034802930344	35.507709062229615	24.146567619128824	13.705688515711218
7	69.2321555047636	18.11389820917995	8.676800727063444	3.977145558993002
8	80.03540364907562	6.6112003038474105	8.920479373547254	4.432916673529717
9	73.54715690482581	8.373050207490188	11.09430605844218	6.985486829241825
10	39.76825821603977	27.485497971605383	17.84929129723272	14.896952515122125
11	31.007938207358414	26.779072122097052	23.83041516446613	18.382574506078402
12	28.492767637150386	23.543426635287187	28.74700730648379	19.21679842107864
13	25.688961405759343	24.488395955031358	30.192510975228103	19.630131663981196
14	20.719757929729475	27.636355885154206	29.894670752845904	21.749215432270415
15	20.614341481469282	27.01635602080037	32.56176502789951	19.807537469830837
16	23.80483617334417	26.48820798820266	29.9523203729882	19.754635465464972
17	24.17495642366948	25.58170404525931	27.656024579085102	22.5873149519861
18	24.83342166482414	26.185135699454605	28.052498941475463	20.928943694245795
19	25.494987389751156	27.46718573932489	26.07477785518222	20.96304901574174
20	26.490339570796156	26.160622499629394	27.86869838784533	19.48033954172912
21	25.776162511856924	27.001822503117438	27.562428725207027	19.6595862598186
22	23.62122939994981	25.206351728568148	28.373592792150347	22.79882607933169
23	23.304786274933463	27.031470879190618	28.145901015117765	21.517841830758154
24	23.195300441722047	25.942232173913887	28.169639093999887	22.69282829036418
25	23.510774665559538	26.541885113511615	27.270014349426457	22.677325871502386
26	22.136097672990037	26.585001215970976	27.44499790232895	23.833903208710034
27	23.102382818669177	26.559131554495362	27.872767772796546	22.465717854038914
28	20.80802482712381	27.052883595243472	29.428726175931136	22.710365401701583
29	22.41620700379906	27.301406747621588	28.398687332682876	21.88369891589647
30	22.774991110331683	25.34403258608445	29.166541516931066	22.714434786652802
31	23.31428150648631	26.582385182788055	26.602344547072608	23.500988763653027
32	23.68740535046609	27.386863831597225	27.369036049906164	21.556694768030525
33	22.92148896857563	26.534327684316494	27.443447660442764	23.10073568666511
34	21.547005756241905	26.71105525934093	29.258490238805074	22.48344874561209
35	21.096660488306814	26.562813378975036	27.928382700463235	24.412143432254915
36	21.529081084432956	27.76318504946725	28.19017979899176	22.517554067108033
37	21.39643851304674	28.114993067512067	27.37165208308909	23.116916336352105
38	21.621320476660625	27.943594448971364	26.752233559442573	23.68285151492544
39	22.580241973380407	25.852899485222803	27.528807854300517	24.03805068709627
40	22.731971897990206	24.55883507073463	29.61204227897184	23.09715075230332
41	20.27997368464398	25.721225815015448	28.763478626524446	25.235321873816126
42	21.281139272762154	26.366707780373353	28.575511797825204	23.776641149039285
43	21.89551951027859	24.52618310100698	28.568535709337397	25.009761679377036
44	20.99541031511573	26.253152562210722	28.207813800447052	24.543623322226495
45	21.845717989685077	25.16061959292586	27.167117044231308	25.826545373157757
46	21.88989988344119	26.208389327747295	27.524254018759862	24.37745677005165
47	20.813644453961206	26.31719693013351	29.201906409959527	23.66725220594576
48	21.010815843859636	25.588001902921913	28.887594867536677	24.513587385681774
49	21.048118539245827	25.238809918060028	29.491317192085624	24.221754350608517
50	19.948415701237384	25.389667831608854	30.387938343004585	24.273978124149185
51	20.43121915866435	24.75067750414932	28.155299356552728	26.662803980633605
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	413.0
1	1316.0
2	2219.0
3	4531.5
4	6844.0
5	4873.0
6	2902.0
7	2784.5
8	2667.0
9	2563.0
10	2459.0
11	2410.0
12	2361.0
13	2315.0
14	2269.0
15	2378.5
16	2488.0
17	2606.5
18	2725.0
19	2778.5
20	2832.0
21	3112.0
22	3392.0
23	3930.5
24	4469.0
25	5591.0
26	7768.0
27	8823.0
28	10902.5
29	12982.0
30	15636.5
31	18291.0
32	21475.5
33	24660.0
34	27518.5
35	30377.0
36	31533.0
37	32689.0
38	36262.0
39	39835.0
40	44430.0
41	49025.0
42	52333.0
43	55641.0
44	62197.0
45	68753.0
46	79545.5
47	90338.0
48	89120.5
49	87903.0
50	85389.0
51	82875.0
52	75523.5
53	68172.0
54	63739.5
55	59307.0
56	56448.5
57	53590.0
58	52535.0
59	51480.0
60	48543.0
61	45606.0
62	40592.5
63	35579.0
64	30476.5
65	25374.0
66	21500.5
67	17627.0
68	14233.0
69	10839.0
70	9039.0
71	7239.0
72	6009.5
73	4780.0
74	3699.5
75	2002.0
76	1385.0
77	1075.5
78	766.0
79	583.5
80	401.0
81	295.0
82	189.0
83	139.0
84	89.0
85	60.5
86	32.0
87	32.5
88	33.0
89	28.0
90	23.0
91	15.0
92	7.0
93	9.0
94	11.0
95	7.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1032097.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.92262032675293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.45524705867471	22.71428715803233
2	8.989915633752508	5.559834958241386
3	3.905908810250798	3.6234280551091396
4	2.3869067458425204	2.9523764422821444
5	1.5790178237602943	2.4413684326657625
6	1.0820986598760909	2.007679560926191
7	0.838741668038368	1.8155263107084527
8	0.6189174049350775	1.5310838341141282
9	0.4966296467390876	1.3821381008210014
>10	6.28310445669998	45.62094592344148
>50	0.31933868211579225	5.967461164255874
>100	0.039265252724412766	2.111258161049483
>500	0.0024540782952129736	0.49402131925122317
>1k	0.0024540782952129736	1.7785905791014658
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTC	4164	0.4034504508781636	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3332	0.32283787279683984	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGC	2845	0.2756523853862573	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCG	2589	0.2508485152073885	No Hit
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	1962	0.19009841129273702	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTT	1470	0.14242847329272346	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCT	1320	0.12789495560979247	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	1068	0.10347864590246848	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.906703536586193E-4	0.0	0.0	0.07576807218701342	0.0
2	2.906703536586193E-4	0.0	0.0	0.4535426418253323	0.0
3	2.906703536586193E-4	0.0	0.0	0.5956804447643972	0.0
4	2.906703536586193E-4	0.0	0.0	0.9258819665205886	0.0
5	2.906703536586193E-4	0.0	0.0	1.8411060200736946	0.0
6	2.906703536586193E-4	0.0	0.0	2.4578116204193985	0.0
7	2.906703536586193E-4	0.0	0.0	2.845759652435769	0.0
8	2.906703536586193E-4	0.0	0.0	3.527090961411573	0.0
9	2.906703536586193E-4	0.0	0.0	3.778327037090506	0.0
10	2.906703536586193E-4	0.0	0.0	4.55848626631024	0.0
11	2.906703536586193E-4	0.0	0.0	5.236038860688482	0.0
12	2.906703536586193E-4	0.0	0.0	6.143899265282236	0.0
13	3.875604715448257E-4	0.0	0.0	6.4723567649164755	0.0
14	3.875604715448257E-4	0.0	0.0	6.634163261786441	0.0
15	3.875604715448257E-4	0.0	0.0	6.86786222612797	0.0
16	3.875604715448257E-4	0.0	0.0	7.237982476453279	0.0
17	3.875604715448257E-4	0.0	0.0	7.667399478924946	0.0
18	3.875604715448257E-4	0.0	0.0	8.161151519673053	0.0
19	3.875604715448257E-4	0.0	0.0	8.466839841604035	0.0
20	3.875604715448257E-4	0.0	0.0	8.787449241689492	0.0
21	3.875604715448257E-4	0.0	0.0	9.154372118124556	0.0
22	3.875604715448257E-4	0.0	0.0	9.558500799827923	0.0
23	3.875604715448257E-4	0.0	0.0	9.917381796478432	0.0
24	3.875604715448257E-4	0.0	0.0	10.226267492299657	0.0
25	3.875604715448257E-4	0.0	0.0	10.507055053933884	0.0
26	3.875604715448257E-4	0.0	0.0	10.794043583112828	0.0
27	3.875604715448257E-4	0.0	0.0	11.074249804039736	0.0
28	3.875604715448257E-4	0.0	0.0	11.37121801536096	0.0
29	3.875604715448257E-4	0.0	0.0	11.673030732576493	0.0
30	3.875604715448257E-4	0.0	0.0	12.032880630405863	0.0
31	4.844505894310322E-4	0.0	0.0	12.36017544862547	0.0
32	4.844505894310322E-4	0.0	0.0	12.69803128969467	0.0
33	5.813407073172386E-4	0.0	0.0	13.048773516442738	0.0
34	5.813407073172386E-4	0.0	0.0	13.39631836930056	0.0
35	5.813407073172386E-4	0.0	0.0	13.766244839390096	0.0
36	5.813407073172386E-4	0.0	0.0	14.115436824251985	0.0
37	5.813407073172386E-4	0.0	0.0	14.47208934819111	0.0
38	5.813407073172386E-4	0.0	0.0	14.849185687004226	0.0
39	5.813407073172386E-4	0.0	0.0	15.336542979971844	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	50	2.1827873E-11	45.0	1
TCGGTTA	25	3.890528E-5	45.0	20
TACGGGT	90	0.0	42.500004	4
ACGGGTA	85	0.0	42.35294	5
CGGTCTA	75	0.0	42.000004	31
GCTAGCG	60	3.6379788E-12	41.250004	1
CACAACG	300	0.0	41.250004	12
CGAATAT	110	0.0	40.909092	14
ATTGCGG	105	0.0	40.714287	2
CGTTTTT	1515	0.0	39.504948	1
GTTACGG	105	0.0	38.57143	2
AATTGCG	35	6.24767E-6	38.571426	1
TCACGAC	90	0.0	37.500004	25
GTTTACG	30	1.139841E-4	37.500004	1
GACCGAC	30	1.139841E-4	37.500004	9
CTCGAAC	30	1.139841E-4	37.500004	13
TAATCGC	30	1.139841E-4	37.500004	36
AACGAGC	335	0.0	36.9403	15
TATTGCG	55	2.748493E-9	36.81818	1
CGGGTAT	105	0.0	36.42857	6
>>END_MODULE