##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936679.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	278089
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.979024700725308	31.0	30.0	33.0	28.0	34.0
2	31.02366508563805	31.0	31.0	33.0	28.0	34.0
3	30.73088831273441	31.0	30.0	34.0	27.0	34.0
4	34.70904278845981	35.0	35.0	37.0	32.0	37.0
5	34.939195725109585	35.0	35.0	37.0	32.0	37.0
6	34.90793594856323	35.0	35.0	37.0	32.0	37.0
7	30.90553024391472	35.0	35.0	37.0	0.0	37.0
8	32.783860562625634	35.0	33.0	37.0	17.0	37.0
9	35.71907914372737	37.0	34.0	39.0	31.0	39.0
10	35.77289285084991	37.0	35.0	39.0	32.0	39.0
11	36.09425399782084	37.0	35.0	39.0	32.0	39.0
12	36.248700955449515	37.0	35.0	39.0	32.0	39.0
13	35.97573438719259	37.0	35.0	39.0	31.0	39.0
14	37.14867182808381	39.0	36.0	40.0	32.0	41.0
15	37.110187745649775	39.0	36.0	40.0	32.0	41.0
16	37.343429621452124	39.0	36.0	40.0	32.0	41.0
17	37.01074116559806	39.0	35.0	40.0	31.0	41.0
18	36.87754999298786	38.0	35.0	40.0	31.0	41.0
19	36.650147974209695	38.0	35.0	40.0	31.0	41.0
20	36.45672788208092	38.0	35.0	40.0	30.0	41.0
21	36.73425414165968	38.0	35.0	40.0	31.0	41.0
22	36.80001726066116	38.0	35.0	40.0	31.0	41.0
23	36.92705572676373	38.0	35.0	40.0	32.0	41.0
24	36.76054428618176	38.0	35.0	40.0	31.0	41.0
25	36.653082286606086	38.0	35.0	40.0	31.0	41.0
26	36.4892570364164	38.0	35.0	40.0	31.0	41.0
27	36.54446238434458	38.0	35.0	40.0	31.0	41.0
28	36.45725289385772	38.0	35.0	40.0	31.0	41.0
29	36.36277594583029	38.0	35.0	40.0	30.0	41.0
30	36.20811682590825	38.0	35.0	40.0	30.0	41.0
31	36.16687463366045	38.0	35.0	40.0	30.0	41.0
32	35.891707331106225	38.0	35.0	40.0	29.0	41.0
33	35.63665229476894	38.0	35.0	40.0	27.0	41.0
34	35.43548648094675	38.0	35.0	40.0	27.0	41.0
35	35.260283578278894	38.0	35.0	40.0	25.0	41.0
36	35.29886834790301	38.0	35.0	40.0	25.0	41.0
37	35.127987802466116	38.0	34.0	40.0	25.0	41.0
38	35.11095368748854	38.0	34.0	40.0	25.0	41.0
39	34.93260790610201	38.0	34.0	40.0	24.0	41.0
40	34.854039534105986	38.0	34.0	40.0	24.0	41.0
41	34.563121878247614	38.0	33.0	40.0	23.0	41.0
42	34.79857527626048	38.0	34.0	40.0	24.0	41.0
43	34.83950821499592	38.0	34.0	40.0	24.0	41.0
44	34.71673097461604	38.0	34.0	40.0	23.0	41.0
45	34.673464250653566	38.0	34.0	40.0	23.0	41.0
46	34.55651607938466	38.0	34.0	40.0	23.0	41.0
47	34.38459989427845	38.0	33.0	40.0	23.0	41.0
48	34.31602112992603	38.0	33.0	40.0	22.0	41.0
49	34.23789506237212	37.0	33.0	40.0	22.0	41.0
50	34.025056726443694	37.0	33.0	40.0	20.0	41.0
51	32.193639446364294	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	19.0
10	21.0
11	18.0
12	3.0
13	6.0
14	9.0
15	15.0
16	33.0
17	71.0
18	170.0
19	397.0
20	701.0
21	1131.0
22	1572.0
23	2017.0
24	2763.0
25	3820.0
26	4753.0
27	5093.0
28	5421.0
29	5831.0
30	7255.0
31	9047.0
32	11770.0
33	15778.0
34	22661.0
35	25522.0
36	27983.0
37	38736.0
38	48853.0
39	36604.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.95479145165756	17.345166475480873	20.90194146478286	13.798100608078709
2	30.213348963820934	33.383916659774385	22.191097094814968	14.211637281589706
3	25.03443142303363	33.603990089503725	28.549852744984523	12.811725742478128
4	24.199087342541418	19.704842694245368	40.55068701027369	15.545382952939526
5	31.547454232278156	23.265573251728764	27.090607683151795	18.096364832841285
6	20.38915598962922	38.2740777233188	28.53510926358108	12.801657023470902
7	58.35326100636846	16.10239887230347	21.907734574183085	3.636605547144979
8	66.79983746210746	12.703487013150466	14.802455329049332	5.694220195692746
9	62.0103635886353	5.518377210173721	15.397588541797768	17.073670659393215
10	36.63719169043004	22.626209594770017	24.28862702228423	16.447971692515708
11	28.116538230566473	23.17315679512674	30.77360125715149	17.9367037171553
12	25.39906288993811	18.76989021500311	34.15848882911586	21.67255806594292
13	20.405337859462257	24.082577879743535	40.79988780570249	14.712196455091714
14	18.947531186059138	30.1162577448227	30.27268248654208	20.66352858257608
15	14.22997673406715	24.8963461337917	44.91080193750921	15.962875194631934
16	17.308487570525983	24.720503148272673	32.24687060617285	25.724138675028495
17	17.74791523576984	24.707557652406244	34.78886255838958	22.755664553434332
18	17.60012082462809	25.08657300360676	35.211748756693	22.101557415072154
19	18.05393237416798	28.655574294560378	30.4877215567678	22.802771774503846
20	19.870257363649767	26.476775420818516	37.17442976888694	16.47853744664478
21	19.27872012197534	31.74091747605982	32.04621542024316	16.93414698172168
22	16.212075990060736	22.894469036891067	34.682421814598925	26.211033158449276
23	17.09704447137427	30.089287961767635	32.44285102970632	20.370816537151775
24	20.88144442966101	23.04873619596604	31.25546138106865	24.814357993304302
25	16.48321220904099	33.887712207243005	28.899021536270762	20.730054047445243
26	15.681310659537054	25.6845829932144	33.19297059574453	25.441135751504014
27	20.071991340901654	27.482928127326144	29.859865007245883	22.58521552452632
28	15.269571971563062	24.228933902455687	39.19500591537242	21.306488210608833
29	21.206520214751393	20.450647094994768	31.384556742625563	26.95827594762828
30	17.901103603522614	26.983088148038938	36.36030191773138	18.755506330707075
31	20.66676495654269	22.248632631999108	33.57594151512645	23.50866089633175
32	26.101715637799412	26.01145676384179	28.67894810654143	19.207879491817366
33	22.322350039016285	23.776560741345396	28.795817166446714	25.1052720531916
34	21.129566433767607	22.213032518366422	33.095879376746296	23.561521671119678
35	17.92879258079248	22.816076867477676	31.79413784795515	27.46099270377469
36	18.736807281122232	26.062519553092717	32.07750036858704	23.12317279719802
37	19.83070168183567	24.091927404535955	37.13847005814685	18.93890085548152
38	21.54993545231922	24.984087827997513	29.88827317873055	23.577703540952715
39	24.715109191661664	23.205520534792818	32.84739777553229	19.231972498013224
40	23.513695255835362	21.400343055640462	31.292859480238338	23.793102208285838
41	15.987327797935194	29.41612217671321	31.678347579372073	22.918202445979524
42	18.01149991549468	23.176033571985947	34.223575905555414	24.588890606963957
43	22.086454336561317	26.734966143932336	27.636476092186314	23.54210342732003
44	21.646307477102656	23.194373024463392	31.667559666150048	23.49175983228391
45	18.544422828662764	22.263016516295142	30.148261887381377	29.044298767660713
46	25.727375048995103	25.910409976662148	27.400939986838747	20.961274987504
47	16.408775607809012	22.18822031795576	39.762809747958386	21.64019432627684
48	20.84620391313572	24.046258571896047	30.02204330268367	25.085494212284555
49	18.20640154770595	20.1316844607302	37.33301209325072	24.32890189831313
50	20.797658303636606	20.997594295351487	32.932981887093696	25.271765513918204
51	19.833218861587476	21.535551568023187	28.774960534217463	29.856269036171874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	71.0
1	199.5
2	328.0
3	2326.0
4	4324.0
5	3132.0
6	1940.0
7	1822.5
8	1705.0
9	1703.5
10	1702.0
11	1633.5
12	1565.0
13	1522.0
14	1479.0
15	1393.5
16	1308.0
17	1265.0
18	1222.0
19	1198.5
20	1175.0
21	1194.0
22	1213.0
23	1380.5
24	1548.0
25	1682.5
26	2195.5
27	2574.0
28	2939.5
29	3305.0
30	3639.0
31	3973.0
32	5068.5
33	6164.0
34	6826.5
35	7489.0
36	8167.5
37	8846.0
38	9721.0
39	10596.0
40	11997.0
41	13398.0
42	14646.5
43	15895.0
44	18836.5
45	21778.0
46	39692.5
47	57607.0
48	41937.5
49	26268.0
50	25286.5
51	24305.0
52	19618.0
53	14931.0
54	12950.5
55	10970.0
56	9237.0
57	7504.0
58	6866.0
59	6228.0
60	5663.0
61	5098.0
62	4584.0
63	4070.0
64	3236.5
65	2403.0
66	1822.5
67	1242.0
68	1064.0
69	886.0
70	710.5
71	535.0
72	402.0
73	269.0
74	237.5
75	150.0
76	94.0
77	62.5
78	31.0
79	20.0
80	9.0
81	7.5
82	6.0
83	6.0
84	6.0
85	5.5
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	278089.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.0076646487908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.89810311044583	29.198345413370973
2	8.704214647921859	6.442453134427698
3	3.0939359511757214	3.4349803237784604
4	1.6629599960157222	2.4616906342761733
5	1.0972559619751536	2.030344033733142
6	0.7464903845487207	1.6575519488955568
7	0.5606170525690697	1.452298951450876
8	0.4816709624292377	1.4260413958913234
9	0.33377199369902155	1.1116909810774533
>10	4.333040012761588	33.129204839366324
>50	0.050965184651848426	1.142386373770176
>100	0.023983616306752203	1.8960877106894443
>500	0.0019986346922293502	0.6923105508895337
>1k	0.007994538768917401	4.843215264128918
>5k	0.0029979520383440253	9.081398444253947
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCG	8944	3.2162365285933645	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGC	8077	2.9044658364768114	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTC	7535	2.709564204265541	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	2383	0.8569199069362685	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2278	0.8191622106591775	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTT	1897	0.6821557127394468	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCT	1665	0.5987291838224453	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCT	1460	0.5250117768052673	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTC	1316	0.47322979333954235	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCG	1059	0.38081333673751927	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGC	1038	0.37326179748210103	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTA	978	0.351685971038049	No Hit
GAATCTATCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTC	894	0.32147981401637604	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	438	0.1575035330415802	No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	427	0.15354796486017067	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGGTCAT	417	0.149951993786162	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	307	0.1103963119720665	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATG	291	0.10464275825365262	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1765621797338262	0.0
2	0.0	0.0	0.0	1.577912107275009	0.0
3	0.0	0.0	0.0	1.8576786568328845	0.0
4	0.0	0.0	0.0	2.7415683468242182	0.0
5	0.0	0.0	0.0	6.418808367105495	0.0
6	0.0	0.0	0.0	7.72126909011144	0.0
7	0.0	0.0	0.0	8.824872612724704	0.0
8	0.0	0.0	0.0	10.239168036132318	0.0
9	0.0	0.0	0.0	10.536195246845434	0.0
10	0.0	0.0	0.0	15.046981362081924	0.0
11	0.0	0.0	0.0	16.23149423386038	0.0
12	0.0	0.0	0.0	20.57219091729626	0.0
13	0.0	0.0	0.0	21.148265483352453	0.0
14	0.0	0.0	0.0	21.428032032910327	0.0
15	0.0	0.0	0.0	22.12924639234202	0.0
16	0.0	0.0	0.0	22.864982074084196	0.0
17	0.0	0.0	0.0	23.7362858653165	0.0
18	0.0	0.0	0.0	24.66045043133673	0.0
19	0.0	0.0	0.0	25.354113251513006	0.0
20	0.0	0.0	0.0	25.88164220807008	0.0
21	0.0	0.0	0.0	26.50806036916239	0.0
22	0.0	0.0	0.0	27.2161070736347	0.0
23	0.0	0.0	0.0	27.938537662403043	0.0
24	0.0	0.0	0.0	28.455997899952894	0.0
25	0.0	0.0	0.0	28.932464067259044	0.0
26	0.0	0.0	0.0	29.388433199443345	0.0
27	0.0	0.0	0.0	29.873170100219713	0.0
28	0.0	0.0	0.0	30.31511494521538	0.0
29	0.0	0.0	0.0	30.777197228225496	0.0
30	0.0	0.0	0.0	31.307243364534376	0.0
31	0.0	0.0	0.0	31.824703602084224	0.0
32	0.0	0.0	0.0	32.3112384883976	0.0
33	0.0	0.0	0.0	32.764690440830094	0.0
34	3.5959710740086806E-4	0.0	0.0	33.20987165979237	0.0
35	3.5959710740086806E-4	0.0	0.0	33.67519031676909	0.0
36	3.5959710740086806E-4	0.0	0.0	34.121450327053566	0.0
37	3.5959710740086806E-4	0.0	0.0	34.575261876593466	0.0
38	3.5959710740086806E-4	0.0	0.0	35.05424522365142	0.0
39	3.5959710740086806E-4	0.0	0.0	35.50338201079511	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCACG	20	7.0240523E-4	45.000004	1
CGGCGAA	20	7.0240523E-4	45.000004	31
ACCGGTG	20	7.0240523E-4	45.000004	17
CATATCC	20	7.0240523E-4	45.000004	34
GTATTAC	20	7.0240523E-4	45.000004	38
CTCCGGG	20	7.0240523E-4	45.000004	3
CCGATAG	20	7.0240523E-4	45.000004	40
CGGTTTT	40	6.7830115E-9	45.000004	1
AGGCGTA	20	7.0240523E-4	45.000004	6
CACGACC	35	1.2074815E-7	45.000004	27
TATTGTC	20	7.0240523E-4	45.000004	19
AGGCCGG	20	7.0240523E-4	45.000004	2
GTACTGG	20	7.0240523E-4	45.000004	2
AAACACG	35	1.2074815E-7	45.000004	40
AGACACG	35	1.2074815E-7	45.000004	24
GTATAAC	20	7.0240523E-4	45.000004	11
CCGGTGA	20	7.0240523E-4	45.000004	18
ACCCGTC	20	7.0240523E-4	45.000004	17
ACCCGGA	20	7.0240523E-4	45.000004	14
CACCGGT	20	7.0240523E-4	45.000004	16
>>END_MODULE