SRR2936676.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936676.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	247956
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	6079	2.4516446466308537	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG	6007	2.4226072367678135	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	4990	2.0124538224523705	RNA PCR Primer, Index 21 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2041	0.8231299101453484	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	1860	0.7501330881285388	TruSeq Adapter, Index 21 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCT	1137	0.45854909742051003	RNA PCR Primer, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	963	0.38837535691816294	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTC	935	0.3770830308603139	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTA	813	0.3278807530368291	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG	510	0.2056816531965349	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	497	0.2004387875268193	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	488	0.19680911129393927	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	447	0.18027391956637467	No Hit
GAATCTATCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	443	0.17866073012953912	RNA PCR Primer, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	398	0.16051234896513897	RNA PCR Primer, Index 21 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	332	0.1338947232573521	RNA PCR Primer, Index 21 (96% over 27bp)
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	315	0.12703866815080095	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	275	0.11090677378244529	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGAT	70	0.0	45.000004	9
CGATTAG	20	7.022574E-4	45.000004	42
TGACCTA	20	7.022574E-4	45.000004	23
AAGCCCG	30	2.158593E-6	45.000004	26
TTGGTAG	20	7.022574E-4	45.000004	1
ATGGTTC	20	7.022574E-4	45.000004	36
TACTAGA	20	7.022574E-4	45.000004	30
TTGCCAG	20	7.022574E-4	45.000004	15
ATTGCGG	20	7.022574E-4	45.000004	2
CCCGATC	20	7.022574E-4	45.000004	41
TCACGGT	25	3.88193E-5	45.0	31
CCGATTA	25	3.88193E-5	45.0	18
TCCTCAC	25	3.88193E-5	45.0	17
CGATTTA	25	3.88193E-5	45.0	10
TAGCCCG	25	3.88193E-5	45.0	13
TCGGCTC	25	3.88193E-5	45.0	45
TACGAGG	50	2.1827873E-11	45.0	2
CGTGTAC	55	1.8189894E-12	45.0	18
ATACGAG	25	3.88193E-5	45.0	1
TTACCGA	25	3.88193E-5	45.0	43

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content