##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936676.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	247956
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99884253657907	31.0	31.0	33.0	28.0	34.0
2	31.019826098178708	31.0	31.0	34.0	28.0	34.0
3	30.830994208649923	31.0	30.0	34.0	27.0	34.0
4	34.829360854345126	35.0	35.0	37.0	32.0	37.0
5	35.0439957089161	35.0	35.0	37.0	33.0	37.0
6	34.996430818371	35.0	35.0	37.0	32.0	37.0
7	31.038502798883673	35.0	35.0	37.0	0.0	37.0
8	32.86053170723838	35.0	33.0	37.0	17.0	37.0
9	35.80860717224024	37.0	34.0	39.0	32.0	39.0
10	35.900849344238495	37.0	35.0	39.0	32.0	39.0
11	36.15410395392731	37.0	35.0	39.0	32.0	39.0
12	36.212844214296084	37.0	35.0	39.0	32.0	39.0
13	35.93629514913936	37.0	35.0	39.0	31.0	39.0
14	37.030711093903754	39.0	36.0	40.0	31.0	41.0
15	37.07229508461179	39.0	35.0	40.0	32.0	41.0
16	37.24412799044992	39.0	36.0	40.0	32.0	41.0
17	37.00273435609544	39.0	35.0	40.0	31.0	41.0
18	36.91353304618561	38.0	35.0	40.0	31.0	41.0
19	36.70402813402378	38.0	35.0	40.0	31.0	41.0
20	36.5874308345029	38.0	35.0	40.0	31.0	41.0
21	36.762651438158386	38.0	35.0	40.0	31.0	41.0
22	36.79262853086838	38.0	35.0	40.0	31.0	41.0
23	36.86593992482537	38.0	35.0	40.0	31.0	41.0
24	36.67207488425366	38.0	35.0	40.0	31.0	41.0
25	36.52726290148252	38.0	35.0	40.0	31.0	41.0
26	36.351380890157934	38.0	35.0	40.0	30.0	41.0
27	36.43504492732582	38.0	35.0	40.0	31.0	41.0
28	36.36237074319637	38.0	35.0	40.0	30.0	41.0
29	36.296596976882995	38.0	35.0	40.0	30.0	41.0
30	36.10326025585184	38.0	35.0	40.0	30.0	41.0
31	36.07016567455516	38.0	35.0	40.0	30.0	41.0
32	35.906418880769166	38.0	35.0	40.0	30.0	41.0
33	35.69414331574957	38.0	35.0	40.0	28.0	41.0
34	35.49534594847473	38.0	34.0	40.0	27.0	41.0
35	35.46651018729129	38.0	34.0	40.0	27.0	41.0
36	35.42833405926858	38.0	35.0	40.0	27.0	41.0
37	35.3004686315314	38.0	34.0	40.0	26.0	41.0
38	35.15503153785349	38.0	34.0	40.0	26.0	41.0
39	35.053049734630335	38.0	34.0	40.0	25.0	41.0
40	34.8732678378422	38.0	34.0	40.0	25.0	41.0
41	34.59338350352482	38.0	33.0	40.0	23.0	41.0
42	34.83939489264224	38.0	34.0	40.0	24.0	41.0
43	34.861854522576586	38.0	34.0	40.0	24.0	41.0
44	34.634870702866635	38.0	34.0	40.0	23.0	41.0
45	34.62615141396054	37.0	34.0	40.0	23.0	41.0
46	34.42411153591766	37.0	33.0	40.0	23.0	41.0
47	34.307715885076384	37.0	33.0	40.0	23.0	41.0
48	34.23961912597397	37.0	33.0	40.0	23.0	41.0
49	34.182536417751535	37.0	33.0	40.0	23.0	41.0
50	34.004896029940795	37.0	33.0	40.0	22.0	41.0
51	32.187343722273305	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	15.0
10	14.0
11	13.0
12	11.0
13	6.0
14	11.0
15	22.0
16	39.0
17	72.0
18	158.0
19	290.0
20	533.0
21	813.0
22	1145.0
23	1564.0
24	2204.0
25	3058.0
26	3936.0
27	4362.0
28	4934.0
29	5593.0
30	6814.0
31	8536.0
32	11006.0
33	14769.0
34	21702.0
35	23474.0
36	24498.0
37	34078.0
38	43000.0
39	31278.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.13394311894046	17.663214441271837	20.14833276871703	14.054509671070676
2	30.324735033634997	33.21436061236671	20.50202455274323	15.95887980125506
3	28.448999015954445	30.547355175918305	26.740228104986368	14.26341770314088
4	26.271999870944846	21.282404942812434	35.3953927309684	17.050202455274324
5	30.508235332075046	26.03728080788527	25.039926438561682	18.414557421478005
6	22.623368662182	38.63467712013422	26.475261739986127	12.266692477697656
7	60.5994611947281	17.118359708980627	18.426656342254272	3.8555227540370067
8	67.18772685476455	13.349142589814322	13.593137492135702	5.869993063285421
9	62.428818016099626	7.578360676894287	14.246479214054105	15.746342092951975
10	41.32709029021278	20.939198890125667	22.482617883818097	15.251092935843454
11	32.76347416477117	22.27733952798077	26.532933262352998	18.42625304489506
12	28.60628498604591	19.75874751972124	30.896207391634	20.73876010259885
13	22.68829953701463	23.865524528545386	36.05236412911969	17.3938118053203
14	19.565164787300972	29.99967736211263	27.90091790478956	22.534239945796834
15	16.500508154672602	24.444256239010148	40.731420090661246	18.323815515656
16	18.246785720047104	24.418445208020778	31.681024052654504	25.65374501927761
17	18.77671845004759	24.616464211392344	33.21113423349304	23.39568310506703
18	19.077578280017423	25.7392440594299	32.00003226378874	23.183145396763944
19	20.882333962477215	26.326041717078834	29.10113084579522	23.690493474648726
20	22.668537966413396	24.99516043168949	34.5791188759296	17.75718272596751
21	22.029311652067303	29.489909500072592	30.027504879898046	18.45327396796206
22	19.65308361160851	22.89277129813354	30.908306312410268	26.54583877784768
23	20.55284002000355	28.650244398199682	30.558647501976154	20.238268079820614
24	22.14626788623788	23.87560696252561	29.21405410637371	24.7640710448628
25	18.899320847247093	30.740131313620157	29.23260578489732	21.12794205423543
26	18.271386858958845	24.87901079223733	30.60381680620755	26.245785542596266
27	21.8478278404233	27.225798125473876	28.58450692864863	22.341867105454195
28	15.85684556937521	25.256093823097647	35.68052396392908	23.206536643598056
29	20.284243978770427	22.842762425591637	29.9754795205601	26.89751407507784
30	19.46474374485796	26.315959283098618	34.792866476310316	19.42643049573311
31	23.145638742357516	23.520705286421784	28.794221555437254	24.539434415783447
32	22.59392795495975	26.35790220845634	28.0114213812128	23.036748455371114
33	21.721999064350126	24.10427656519705	26.325638419719628	27.848085950733193
34	21.17916081885496	26.546242075206894	28.553453031989545	23.721144073948604
35	19.82811466550517	21.702237493748893	31.73950216973979	26.730145671006145
36	21.585281259578313	24.111132620303604	28.792608366000422	25.510977754117665
37	18.725096388068852	24.653970865798772	34.18146767974963	22.439465066382745
38	21.84097178531675	25.478713965380955	28.707915920566553	23.972398328735743
39	21.901869686557294	25.21536078981755	27.456080917582153	25.426688606043008
40	23.389633644678895	22.764522737905114	32.94576457113359	20.900079046282404
41	17.257094000548484	27.593202019713175	28.39616706189808	26.753536917840258
42	19.196147703624835	22.875026214328347	35.11752085047347	22.811305231573346
43	23.285582926003002	21.434448047234188	26.98059333107487	28.299375695687946
44	20.995660520414912	23.55175918308087	30.32392843891658	25.128651857587634
45	19.786171740147445	23.066592459952574	28.737356627788802	28.40987917211118
46	25.22544322379777	26.007033505944605	27.154817790253112	21.612705480004518
47	18.74324476923325	22.369291325880397	37.02592395424995	21.861539950636402
48	21.168271790156318	24.637838971430416	28.481262804691156	25.712626433722114
49	19.458694284469825	21.589717530529608	35.131232960686575	23.820355224313992
50	21.16020584297214	21.198519092096983	31.5447095452419	26.096565519688973
51	20.485892658374873	21.992208295020085	27.87591346851861	29.645985578086435
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	333.5
2	556.0
3	2112.0
4	3668.0
5	2567.0
6	1466.0
7	1318.5
8	1171.0
9	1136.5
10	1102.0
11	1102.0
12	1102.0
13	1058.5
14	1015.0
15	1005.5
16	996.0
17	887.0
18	778.0
19	751.0
20	724.0
21	846.5
22	969.0
23	1087.5
24	1206.0
25	1308.0
26	1661.5
27	1913.0
28	2568.5
29	3224.0
30	3675.5
31	4127.0
32	4224.0
33	4321.0
34	4825.0
35	5329.0
36	5825.0
37	6321.0
38	7016.5
39	7712.0
40	9054.0
41	10396.0
42	11706.5
43	13017.0
44	15149.5
45	17282.0
46	29548.5
47	41815.0
48	32398.5
49	22982.0
50	21946.5
51	20911.0
52	17657.0
53	14403.0
54	12821.5
55	11240.0
56	10956.5
57	10673.0
58	9380.5
59	8088.0
60	7878.5
61	7669.0
62	6950.5
63	6232.0
64	5314.0
65	4396.0
66	3786.5
67	3177.0
68	2672.0
69	2167.0
70	1851.5
71	1536.0
72	1315.0
73	1094.0
74	929.5
75	591.5
76	418.0
77	366.5
78	315.0
79	195.0
80	75.0
81	50.0
82	25.0
83	23.5
84	22.0
85	19.0
86	16.0
87	12.5
88	9.0
89	7.5
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	247956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.696075110100175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67504736664691	26.2562712739357
2	8.680591879671281	6.023649357144009
3	3.4743290209343143	3.6163674200261333
4	1.945810231195732	2.70047911726274
5	1.354163034255094	2.349207117391795
6	0.9391963362043914	1.9551855974447079
7	0.7648405807209029	1.8575876365161563
8	0.6265183480373354	1.739018212908742
9	0.5428275854052609	1.6950588007549727
>10	5.869977101277446	37.07109325848134
>50	0.07671653241273495	1.7962864379164045
>100	0.038358266206367474	2.72225717466002
>500	0.004649486812893027	1.2990207940118408
>1k	0.004649486812893027	4.044265918146768
>5k	0.0023247434064465134	4.874251883398668
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	6079	2.4516446466308537	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG	6007	2.4226072367678135	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	4990	2.0124538224523705	RNA PCR Primer, Index 21 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2041	0.8231299101453484	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	1860	0.7501330881285388	TruSeq Adapter, Index 21 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCT	1137	0.45854909742051003	RNA PCR Primer, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	963	0.38837535691816294	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTC	935	0.3770830308603139	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTA	813	0.3278807530368291	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG	510	0.2056816531965349	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	497	0.2004387875268193	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	488	0.19680911129393927	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	447	0.18027391956637467	No Hit
GAATCTATCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	443	0.17866073012953912	RNA PCR Primer, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	398	0.16051234896513897	RNA PCR Primer, Index 21 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	332	0.1338947232573521	RNA PCR Primer, Index 21 (96% over 27bp)
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	315	0.12703866815080095	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	275	0.11090677378244529	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.032973592088919E-4	0.0	0.0	0.15607607801384116	0.0
2	4.032973592088919E-4	0.0	0.0	1.3974253496588105	0.0
3	4.032973592088919E-4	0.0	0.0	1.626498249689461	0.0
4	4.032973592088919E-4	0.0	0.0	2.353643388343093	0.0
5	4.032973592088919E-4	0.0	0.0	5.346109793673071	0.0
6	4.032973592088919E-4	0.0	0.0	5.931294261885173	0.0
7	4.032973592088919E-4	0.0	0.0	6.879849650744487	0.0
8	4.032973592088919E-4	0.0	0.0	8.055864750197616	0.0
9	4.032973592088919E-4	0.0	0.0	8.328897062382035	0.0
10	4.032973592088919E-4	0.0	0.0	11.728290503153785	0.0
11	4.032973592088919E-4	0.0	0.0	12.564325928793819	0.0
12	4.032973592088919E-4	0.0	0.0	16.18593621448967	0.0
13	4.032973592088919E-4	0.0	0.0	16.620287470357646	0.0
14	4.032973592088919E-4	0.0	0.0	16.86105599380535	0.0
15	4.032973592088919E-4	0.0	0.0	17.478100953394957	0.0
16	4.032973592088919E-4	0.0	0.0	18.050783203471585	0.0
17	4.032973592088919E-4	0.0	0.0	18.640403942634983	0.0
18	4.032973592088919E-4	0.0	0.0	19.280436851699495	0.0
19	4.032973592088919E-4	0.0	0.0	19.765603574827793	0.0
20	4.032973592088919E-4	0.0	0.0	20.161641581570922	0.0
21	4.032973592088919E-4	0.0	0.0	20.633096194486118	0.0
22	4.032973592088919E-4	0.0	0.0	21.133184919905144	0.0
23	4.032973592088919E-4	0.0	0.0	21.57963509654939	0.0
24	4.032973592088919E-4	0.0	0.0	21.962364290438625	0.0
25	4.032973592088919E-4	0.0	0.0	22.29548790914517	0.0
26	4.032973592088919E-4	0.0	0.0	22.62417525690042	0.0
27	4.032973592088919E-4	0.0	0.0	22.950039523141204	0.0
28	4.032973592088919E-4	0.0	0.0	23.27550049202278	0.0
29	4.032973592088919E-4	0.0	0.0	23.609027408088533	0.0
30	4.032973592088919E-4	0.0	0.0	23.976431302327832	0.0
31	4.032973592088919E-4	0.0	0.0	24.321653841810644	0.0
32	4.032973592088919E-4	0.0	0.0	24.677765409992094	0.0
33	4.032973592088919E-4	0.0	0.0	25.0544451434932	0.0
34	4.032973592088919E-4	0.0	0.0	25.37829292293794	0.0
35	4.032973592088919E-4	0.0	0.0	25.754569359079838	0.0
36	4.032973592088919E-4	0.0	0.0	26.10745454838762	0.0
37	4.032973592088919E-4	0.0	0.0	26.440981464453372	0.0
38	4.032973592088919E-4	0.0	0.0	26.767652325412573	0.0
39	4.032973592088919E-4	0.0	0.0	27.111664972817756	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGAT	70	0.0	45.000004	9
CGATTAG	20	7.022574E-4	45.000004	42
TGACCTA	20	7.022574E-4	45.000004	23
AAGCCCG	30	2.158593E-6	45.000004	26
TTGGTAG	20	7.022574E-4	45.000004	1
ATGGTTC	20	7.022574E-4	45.000004	36
TACTAGA	20	7.022574E-4	45.000004	30
TTGCCAG	20	7.022574E-4	45.000004	15
ATTGCGG	20	7.022574E-4	45.000004	2
CCCGATC	20	7.022574E-4	45.000004	41
TCACGGT	25	3.88193E-5	45.0	31
CCGATTA	25	3.88193E-5	45.0	18
TCCTCAC	25	3.88193E-5	45.0	17
CGATTTA	25	3.88193E-5	45.0	10
TAGCCCG	25	3.88193E-5	45.0	13
TCGGCTC	25	3.88193E-5	45.0	45
TACGAGG	50	2.1827873E-11	45.0	2
CGTGTAC	55	1.8189894E-12	45.0	18
ATACGAG	25	3.88193E-5	45.0	1
TTACCGA	25	3.88193E-5	45.0	43
>>END_MODULE