FastQCFastQC Report
Sat 14 Jan 2017
SRR2936675.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936675.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287558
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC91723.1896173989247387No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG90883.160405900722637No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC60702.1108785010328353No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22640.78731942773284No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC21030.7313307228454781No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCT16760.5828389403181271No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTA11930.4148728256560415No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTC11880.4131340460011545No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT10400.36166616821649894No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC6820.2371695449265887No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG6770.2354307652717017No Hit
GAATCTATCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC4680.16274977569742452No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTACCGT3690.12832193853066165No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3440.11962804025622657No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT3280.11406394536058813No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATG3150.10954311825788188No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTAT3030.10537004708615307No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGT2980.10363126743126604No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2900.10084921998344681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTT302.1598062E-645.00000427
CCTACGA302.1598062E-645.00000428
TAGGGCG302.1598062E-645.0000045
AGCTACG600.045.0000049
CTTAACA207.024456E-445.038
TTAGGAC207.024456E-445.029
CTTTTAG207.024456E-445.015
TGTATCG207.024456E-445.04
AGGCGAT351.2076816E-745.07
ACGGGAA351.2076816E-745.05
ACGATGG207.024456E-445.02
TACGATC207.024456E-445.013
GTATGCG207.024456E-445.01
CATACGA406.7848305E-945.018
GTATCGC207.024456E-445.05
ATCGCCA207.024456E-445.07
CTTACGA207.024456E-445.011
TCTATAC207.024456E-445.040
ACGGTAG207.024456E-445.01
GCGAAGT253.8834885E-544.99999643
Produced by FastQC (version 0.11.5)