##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936675.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	287558
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.082449453675434	31.0	31.0	33.0	28.0	34.0
2	31.09755249375778	31.0	31.0	34.0	28.0	34.0
3	30.877868117040737	31.0	30.0	34.0	28.0	34.0
4	34.88416597695074	35.0	35.0	37.0	32.0	37.0
5	35.08155224337351	35.0	35.0	37.0	33.0	37.0
6	35.034664311199826	35.0	35.0	37.0	32.0	37.0
7	31.11648084908088	35.0	35.0	37.0	0.0	37.0
8	32.900562669096324	35.0	35.0	37.0	17.0	37.0
9	35.77055759185973	37.0	34.0	39.0	32.0	39.0
10	35.97554928049298	37.0	35.0	39.0	32.0	39.0
11	36.228896431328636	37.0	35.0	39.0	32.0	39.0
12	36.2526133858213	37.0	35.0	39.0	32.0	39.0
13	35.9007678450956	37.0	35.0	39.0	30.0	39.0
14	37.08817003874001	39.0	36.0	40.0	32.0	41.0
15	37.07405114794233	39.0	35.0	40.0	31.0	41.0
16	37.365515826372416	39.0	36.0	40.0	32.0	41.0
17	37.00331759158153	39.0	35.0	40.0	31.0	41.0
18	36.972805486197565	39.0	35.0	40.0	31.0	41.0
19	36.72057115434104	38.0	35.0	40.0	31.0	41.0
20	36.548804067353366	38.0	35.0	40.0	31.0	41.0
21	36.850670125678995	38.0	35.0	40.0	31.0	41.0
22	36.866534751250185	39.0	35.0	40.0	31.0	41.0
23	36.977016810521704	39.0	35.0	40.0	32.0	41.0
24	36.78996932792689	38.0	35.0	40.0	31.0	41.0
25	36.66782005717108	38.0	35.0	40.0	31.0	41.0
26	36.4646332218196	38.0	35.0	40.0	31.0	41.0
27	36.527806564240954	38.0	35.0	40.0	31.0	41.0
28	36.45269128314983	38.0	35.0	40.0	30.0	41.0
29	36.32705054284701	38.0	35.0	40.0	30.0	41.0
30	36.242267646874716	38.0	35.0	40.0	30.0	41.0
31	36.266304536823874	38.0	35.0	40.0	30.0	41.0
32	35.95184623623756	38.0	35.0	40.0	30.0	41.0
33	35.71747612655534	38.0	35.0	40.0	29.0	41.0
34	35.581698300864524	38.0	35.0	40.0	27.0	41.0
35	35.53091550226389	38.0	35.0	40.0	27.0	41.0
36	35.426049701277655	38.0	35.0	40.0	27.0	41.0
37	35.24072013298187	38.0	34.0	40.0	26.0	41.0
38	35.17563413294014	38.0	34.0	40.0	26.0	41.0
39	35.03172925114238	38.0	34.0	40.0	25.0	41.0
40	34.94220644183086	38.0	34.0	40.0	24.0	41.0
41	34.714384576328946	38.0	34.0	40.0	24.0	41.0
42	34.69548751903964	38.0	34.0	40.0	23.0	41.0
43	34.61981235089964	38.0	34.0	40.0	23.0	41.0
44	34.48353027910891	38.0	33.0	40.0	23.0	41.0
45	34.477142002656855	37.0	33.0	40.0	23.0	41.0
46	34.265546428894346	37.0	33.0	40.0	22.0	41.0
47	34.051200105717804	37.0	33.0	40.0	22.0	41.0
48	34.040350120671306	37.0	33.0	40.0	22.0	41.0
49	33.9731393318913	37.0	33.0	40.0	22.0	41.0
50	33.81731685433895	37.0	33.0	40.0	20.0	41.0
51	31.975757934051565	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	19.0
10	28.0
11	22.0
12	10.0
13	5.0
14	17.0
15	18.0
16	32.0
17	73.0
18	213.0
19	424.0
20	650.0
21	1067.0
22	1459.0
23	2054.0
24	2691.0
25	3609.0
26	4571.0
27	5138.0
28	5762.0
29	6366.0
30	7559.0
31	9651.0
32	12542.0
33	16747.0
34	24755.0
35	26623.0
36	27610.0
37	38483.0
38	50526.0
39	38820.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.654128906168495	16.81052170344765	19.30636602007247	13.228983370311381
2	29.170463002246507	35.62133552187732	19.972666383825178	15.235535092050995
3	27.240417585321918	34.381933383873864	25.08815612850277	13.289492902301447
4	25.280117402402297	20.862226055265374	37.681789412918434	16.17586712941389
5	33.161657822074154	24.85272536323107	23.567071686407612	18.418545128287164
6	21.64189485251671	40.86375618136167	25.426522649343784	12.067826316777833
7	58.57114043080005	16.931888523358765	20.88413467891695	3.6128363669242383
8	65.37046439327023	15.835761828917994	13.674110961962457	5.119662815849324
9	60.28522941458767	7.817553328372015	13.759658920982897	18.13755833605742
10	41.04737131291774	22.6823805980011	21.29622545712517	14.974022631955988
11	34.863923104208546	22.686553669172827	25.879300871476364	16.570222355142267
12	30.899157735135173	19.677073842494384	28.4502604691923	20.973507953178142
13	23.061782318697446	24.167993935136565	35.9617885783042	16.808435167861788
14	19.500066073626883	30.630342400489642	26.965690399849766	22.903901126033706
15	16.780266937452616	24.37038788696541	41.26158896639982	17.58775620918215
16	17.78145626273656	24.138434681003485	31.477128092419616	26.602980963840338
17	18.113215420889002	23.788939970371196	34.811411958630956	23.28643265010885
18	19.211080894984665	25.58892466911023	31.148846493576947	24.051147942328157
19	21.075052685023543	28.24960529701834	27.56974245195752	23.1055995660006
20	24.70284255697981	24.909409579980387	34.31099117395447	16.076756689085332
21	22.176047962498	32.31452437421321	27.98948386064724	17.519943802641556
22	18.988864855090103	23.635231848879183	30.943670494300278	26.432232801730432
23	20.385800429826332	31.296642764242343	29.6291530752057	18.68840373072563
24	22.860083878730553	23.380326751472747	28.168230409169624	25.591358960627076
25	18.781950076158548	33.790748301212275	26.964647132056836	20.462654490572337
26	18.300655867685823	26.794594481808886	30.25650477468893	24.648244875816356
27	22.38748356853226	30.023160545003098	25.746805861773975	21.84255002469067
28	17.85100744893204	27.083231904520133	34.55546359343158	20.51029705311624
29	21.55599913756529	27.089143755346747	29.459100424957747	21.895756682130212
30	18.630328490252403	32.840331341851034	30.39908470638967	18.1302554615069
31	27.015071742048562	24.46741179170811	24.183295196099568	24.334221270143765
32	25.29611417522726	29.722003908776664	27.157999429680274	17.823882486315803
33	22.08910897975365	28.63630989226521	24.03236912205538	25.24221200592576
34	22.114495162715002	29.053269253507118	26.233316409211362	22.59891917456652
35	20.087773596978696	27.648683048289392	25.102066365741866	27.161476988990046
36	27.13748182975261	30.718672406957904	23.124726142204356	19.01911962108514
37	19.488242371973655	29.81589801014056	31.590496525918248	19.105363091967533
38	23.57402680502716	29.965780816391824	24.276146029670535	22.18404634891048
39	22.82739482121868	24.050104674535223	29.606548939692168	23.515951564553934
40	21.066011030818128	27.516188038586996	27.854902315358988	23.562898615235884
41	21.472537714130716	26.318168856369844	25.44217166623777	26.76712176326167
42	19.011121234672657	24.233024294229338	31.505296322828784	25.25055814826922
43	27.501234533554967	23.822672295676004	24.81134240744476	23.86475076332427
44	22.701507174204856	24.94522844087106	28.93259794545796	23.420666439466125
45	19.345662440272918	24.28623095166888	27.466806696388208	28.90129991166999
46	25.181354718004716	29.957782429979346	23.79937264830052	21.061490203715426
47	18.46201461965934	24.213202206163626	35.95622448340857	21.36855869076847
48	22.03277251893531	26.306692910647588	26.141856599364306	25.518677971052796
49	19.122750888516403	22.797487811154618	34.630926630453686	23.448834669875296
50	21.41411471772651	23.54446755089408	29.435105265720306	25.606312465659105
51	20.828493729960567	24.14330326403717	26.377982876497956	28.650220129504312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	351.0
2	629.0
3	2539.0
4	4449.0
5	3096.5
6	1744.0
7	1609.5
8	1475.0
9	1425.0
10	1375.0
11	1321.0
12	1267.0
13	1193.0
14	1119.0
15	1044.5
16	970.0
17	1020.0
18	1070.0
19	1065.0
20	1060.0
21	1161.5
22	1263.0
23	1271.5
24	1280.0
25	1472.5
26	2204.0
27	2743.0
28	2740.5
29	2738.0
30	3769.0
31	4800.0
32	5296.0
33	5792.0
34	6921.5
35	8051.0
36	8069.5
37	8088.0
38	9106.5
39	10125.0
40	11171.5
41	12218.0
42	13667.5
43	15117.0
44	17574.0
45	20031.0
46	36261.0
47	52491.0
48	37427.5
49	22364.0
50	21274.0
51	20184.0
52	17509.0
53	14834.0
54	14008.0
55	13182.0
56	12212.5
57	11243.0
58	10984.5
59	10726.0
60	10292.5
61	9859.0
62	9129.5
63	8400.0
64	6977.5
65	5555.0
66	4486.5
67	3418.0
68	2856.5
69	2295.0
70	1855.0
71	1415.0
72	1243.0
73	1071.0
74	819.0
75	460.0
76	353.0
77	274.5
78	196.0
79	178.0
80	160.0
81	106.0
82	52.0
83	37.5
84	23.0
85	14.5
86	6.0
87	5.0
88	4.0
89	6.5
90	9.0
91	8.5
92	8.0
93	4.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	287558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.72667079337038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.61257172606585	23.233225992669304
2	8.092171531401021	4.9729098129768605
3	3.389657865251197	3.1245870398319644
4	2.011159274307638	2.4718491573873793
5	1.437350747535566	2.2082501617065082
6	0.9970913453376643	1.8382378511465514
7	0.7616827189696346	1.6382781908345447
8	0.6394513168170037	1.571856808017861
9	0.48213497515759934	1.3332962393673624
>10	6.417148613013117	40.752474283448905
>50	0.10751836300462894	2.2002517752940274
>100	0.03961202847538961	2.430118445670091
>500	0.0022635444843079775	0.4726003101982904
>1k	0.0067906334529239335	3.29116213077014
>5k	0.0033953167264619668	8.460901800680212
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	9172	3.1896173989247387	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	9088	3.160405900722637	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	6070	2.1108785010328353	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2264	0.78731942773284	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	2103	0.7313307228454781	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCT	1676	0.5828389403181271	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTA	1193	0.4148728256560415	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTC	1188	0.4131340460011545	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	1040	0.36166616821649894	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	682	0.2371695449265887	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	677	0.2354307652717017	No Hit
GAATCTATCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	468	0.16274977569742452	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTACCGT	369	0.12832193853066165	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	344	0.11962804025622657	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT	328	0.11406394536058813	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATG	315	0.10954311825788188	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTAT	303	0.10537004708615307	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGT	298	0.10363126743126604	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	290	0.10084921998344681	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.477559309774028E-4	0.0	0.0	0.12067130804915878	0.0
2	3.477559309774028E-4	0.0	0.0	1.2032355211818138	0.0
3	3.477559309774028E-4	0.0	0.0	1.3757224629466056	0.0
4	3.477559309774028E-4	0.0	0.0	1.9867296336739022	0.0
5	3.477559309774028E-4	0.0	0.0	4.923180714847092	0.0
6	3.477559309774028E-4	0.0	0.0	5.372481377669896	0.0
7	3.477559309774028E-4	0.0	0.0	6.314899950618658	0.0
8	3.477559309774028E-4	0.0	0.0	7.2135012762642665	0.0
9	3.477559309774028E-4	0.0	0.0	7.411722156921386	0.0
10	3.477559309774028E-4	0.0	0.0	11.578881477823604	0.0
11	3.477559309774028E-4	0.0	0.0	12.159633882555868	0.0
12	3.477559309774028E-4	0.0	0.0	16.40538604385898	0.0
13	3.477559309774028E-4	0.0	0.0	16.785483276417278	0.0
14	3.477559309774028E-4	0.0	0.0	17.001787465485222	0.0
15	3.477559309774028E-4	0.0	0.0	17.70147239861176	0.0
16	3.477559309774028E-4	0.0	0.0	18.185200898601327	0.0
17	3.477559309774028E-4	0.0	0.0	18.63450156142413	0.0
18	3.477559309774028E-4	0.0	0.0	19.060154820940472	0.0
19	3.477559309774028E-4	0.0	0.0	19.49937056176493	0.0
20	3.477559309774028E-4	0.0	0.0	19.813046411506548	0.0
21	3.477559309774028E-4	0.0	0.0	20.152108444209517	0.0
22	3.477559309774028E-4	0.0	0.0	20.52212075476947	0.0
23	3.477559309774028E-4	0.0	0.0	20.878222828090333	0.0
24	3.477559309774028E-4	0.0	0.0	21.152254501700526	0.0
25	3.477559309774028E-4	0.0	0.0	21.412375938071623	0.0
26	3.477559309774028E-4	0.0	0.0	21.64572016775746	0.0
27	3.477559309774028E-4	0.0	0.0	21.909319163438333	0.0
28	3.477559309774028E-4	0.0	0.0	22.133969494849733	0.0
29	3.477559309774028E-4	0.0	0.0	22.38365825329151	0.0
30	3.477559309774028E-4	0.0	0.0	22.675425479381552	0.0
31	3.477559309774028E-4	0.0	0.0	22.958498807197156	0.0
32	3.477559309774028E-4	0.0	0.0	23.223836582532915	0.0
33	3.477559309774028E-4	0.0	0.0	23.47908943587033	0.0
34	3.477559309774028E-4	0.0	0.0	23.74651374679195	0.0
35	3.477559309774028E-4	0.0	0.0	24.04245404405372	0.0
36	3.477559309774028E-4	0.0	0.0	24.330048198972033	0.0
37	3.477559309774028E-4	0.0	0.0	24.598168021755612	0.0
38	3.477559309774028E-4	0.0	0.0	24.879850325847308	0.0
39	3.477559309774028E-4	0.0	0.0	25.161532629939003	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTT	30	2.1598062E-6	45.000004	27
CCTACGA	30	2.1598062E-6	45.000004	28
TAGGGCG	30	2.1598062E-6	45.000004	5
AGCTACG	60	0.0	45.000004	9
CTTAACA	20	7.024456E-4	45.0	38
TTAGGAC	20	7.024456E-4	45.0	29
CTTTTAG	20	7.024456E-4	45.0	15
TGTATCG	20	7.024456E-4	45.0	4
AGGCGAT	35	1.2076816E-7	45.0	7
ACGGGAA	35	1.2076816E-7	45.0	5
ACGATGG	20	7.024456E-4	45.0	2
TACGATC	20	7.024456E-4	45.0	13
GTATGCG	20	7.024456E-4	45.0	1
CATACGA	40	6.7848305E-9	45.0	18
GTATCGC	20	7.024456E-4	45.0	5
ATCGCCA	20	7.024456E-4	45.0	7
CTTACGA	20	7.024456E-4	45.0	11
TCTATAC	20	7.024456E-4	45.0	40
ACGGTAG	20	7.024456E-4	45.0	1
GCGAAGT	25	3.8834885E-5	44.999996	43
>>END_MODULE