##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936674.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	280638
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.146441322985485	31.0	31.0	33.0	28.0	34.0
2	31.188819760688148	31.0	31.0	34.0	28.0	34.0
3	30.981638267091412	31.0	31.0	34.0	28.0	34.0
4	34.96077865435187	35.0	35.0	37.0	32.0	37.0
5	35.1867958009963	35.0	35.0	37.0	33.0	37.0
6	35.141417056849036	36.0	35.0	37.0	32.0	37.0
7	30.953260071693784	35.0	35.0	37.0	0.0	37.0
8	33.005113348869365	35.0	35.0	37.0	17.0	37.0
9	36.27225821164632	39.0	35.0	39.0	32.0	39.0
10	36.117607023995326	37.0	35.0	39.0	32.0	39.0
11	36.3318260534924	37.0	35.0	39.0	32.0	39.0
12	36.37768584439741	38.0	35.0	39.0	32.0	39.0
13	36.25314105716261	38.0	35.0	39.0	32.0	39.0
14	37.261261839095205	39.0	36.0	40.0	32.0	41.0
15	37.41753433248527	39.0	36.0	41.0	32.0	41.0
16	37.42035647346403	39.0	36.0	40.0	32.0	41.0
17	37.32107554928413	39.0	36.0	40.0	32.0	41.0
18	37.23804687889737	39.0	36.0	40.0	32.0	41.0
19	37.05557337210214	39.0	35.0	40.0	32.0	41.0
20	37.02893050834171	39.0	35.0	40.0	31.0	41.0
21	37.05023553474583	39.0	35.0	40.0	32.0	41.0
22	37.168936494701356	39.0	35.0	40.0	32.0	41.0
23	37.21943571433662	39.0	35.0	40.0	32.0	41.0
24	37.140469216570814	39.0	35.0	40.0	32.0	41.0
25	36.85582850504921	39.0	35.0	40.0	31.0	41.0
26	36.81862042916497	39.0	35.0	40.0	31.0	41.0
27	36.84831704900976	39.0	35.0	40.0	31.0	41.0
28	36.85586770145169	39.0	35.0	40.0	31.0	41.0
29	36.83811173112693	39.0	35.0	40.0	31.0	41.0
30	36.61756070097421	39.0	35.0	40.0	31.0	41.0
31	36.58667037250836	39.0	35.0	40.0	31.0	41.0
32	36.41966519145661	39.0	35.0	40.0	30.0	41.0
33	36.27953448927087	38.0	35.0	40.0	30.0	41.0
34	36.19536555990279	38.0	35.0	40.0	30.0	41.0
35	36.115818242718376	39.0	35.0	40.0	30.0	41.0
36	36.05581567713567	38.0	35.0	40.0	30.0	41.0
37	35.93567514021622	38.0	35.0	40.0	29.0	41.0
38	35.74661307449455	38.0	35.0	40.0	29.0	41.0
39	35.68854182256145	38.0	35.0	40.0	28.0	41.0
40	35.54173704202567	38.0	35.0	40.0	27.0	41.0
41	35.40338799449825	38.0	34.0	40.0	26.0	41.0
42	35.41736329363807	38.0	34.0	40.0	27.0	41.0
43	35.37787113648187	38.0	34.0	40.0	27.0	41.0
44	35.19329171388051	38.0	34.0	40.0	26.0	41.0
45	35.11440004561036	38.0	34.0	40.0	26.0	41.0
46	34.96726744061745	37.0	34.0	40.0	26.0	41.0
47	34.85390431801823	37.0	34.0	40.0	25.0	41.0
48	34.77192326057056	37.0	34.0	40.0	25.0	41.0
49	34.67954090322765	37.0	34.0	40.0	24.0	41.0
50	34.531706326299364	37.0	34.0	40.0	24.0	41.0
51	32.768769731825344	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	18.0
10	32.0
11	18.0
12	14.0
13	10.0
14	11.0
15	18.0
16	41.0
17	78.0
18	182.0
19	295.0
20	495.0
21	737.0
22	1048.0
23	1433.0
24	2062.0
25	2756.0
26	3659.0
27	4067.0
28	4570.0
29	5451.0
30	6746.0
31	8479.0
32	10999.0
33	15192.0
34	23024.0
35	24261.0
36	28096.0
37	41217.0
38	53238.0
39	42385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.48243644837834	18.788261033787297	21.977779203101505	13.751523314732857
2	32.413286867779846	25.97011096145212	24.512361120019385	17.10424105074865
3	31.49965435899629	25.397130823338248	27.142439726622907	15.960775091042553
4	28.600189568055644	24.614984428338285	28.734882660224205	18.049943343381866
5	26.828512175827935	28.52856705079141	25.594181828547807	19.048738944832845
6	26.06275700368446	35.41394964331273	25.2239539905501	13.299339362452697
7	67.80193701494453	17.78305147556639	10.695272913860562	3.7197385956285323
8	78.33507935489848	7.949743085398271	9.242155374539442	4.4730221851638055
9	72.95982725076433	7.246345826295797	11.242953555826366	8.550873367113505
10	42.165351805528836	25.241414206201583	17.98687276847754	14.606361219792044
11	31.960033922704696	25.306622766695885	23.973945082276813	18.759398228322606
12	28.25276690968436	22.873595165301918	29.19419323113762	19.679444693876096
13	25.16872269614236	25.204355789308647	30.751715733435958	18.875205781113035
14	20.785495905757596	27.70579892958188	30.22648394016491	21.282221224495615
15	19.37157476891939	25.146273847447603	35.45528403138563	20.02686735224738
16	23.15082063013562	25.82401527947035	29.633905600809584	21.391258489584448
17	24.558684141135558	24.824863347087707	27.881113747960008	22.73533876381673
18	24.782103635288163	24.348092560522808	30.463087678789048	20.40671612539998
19	24.66059478759113	26.202082397964638	26.74406174502384	22.393261069420394
20	25.267782695144636	26.662461961673046	28.7819183432037	19.28783699997862
21	24.4172207612654	27.347686343260712	29.28968992082327	18.94540297465062
22	25.098881833536442	23.021472501942004	28.998567549654712	22.881078114866842
23	22.93025178343631	27.02627584290082	29.572616680563574	20.470855693099296
24	23.171487824172065	24.866910397023926	28.981463664934896	22.98013811386911
25	22.832973439092356	27.6135092182812	27.75283461256138	21.800682730065066
26	21.724784241620878	25.621262979354185	27.958081229199184	24.695871549825753
27	21.713737982739328	26.730164838688985	29.47997063833123	22.076126540240452
28	18.91440218359595	26.38915613708764	31.256280332670556	23.44016134664586
29	23.128728112372524	25.233574925705	28.555291870666128	23.08240509125635
30	22.40501999016527	25.296645500609326	31.087023140130704	21.211311369094705
31	24.719389391315502	24.249032561520536	28.31619381552035	22.71538423164361
32	26.12725290231544	26.19709376492136	27.2308097976753	20.444843535087905
33	23.217810845288238	24.77747133317655	28.180788061488464	23.82392976004675
34	21.88370783714251	25.303059457379256	29.16746841126291	23.645764294215326
35	22.302396681846364	25.93269621362752	29.178158339212796	22.58674876531332
36	23.7102601928463	26.9564349802949	27.690476699520378	21.64282812733842
37	23.85136724178479	26.203507721691288	28.670030430661562	21.275094605862357
38	22.5265288378623	27.67907410970717	28.110947198882545	21.683449853547987
39	23.811458177438553	24.70299816845901	27.571462168344986	23.914081485757453
40	25.086766581859905	23.831056378680007	29.13575495834491	21.946422081115173
41	20.226056343046913	26.16965628318332	27.79951396460921	25.804773409160553
42	23.074209479828106	24.804552483982924	29.272586036103448	22.84865200008552
43	22.65552063512425	25.429913269051234	28.084222379007834	23.83034371681668
44	22.682245454998966	24.487061623871323	29.10475416729025	23.725938753839465
45	22.380076824948866	24.40225486213556	27.968058495285742	25.249609817629832
46	24.581132989830316	25.527547944326855	27.39008972412859	22.50122934171424
47	21.378430576044586	24.92036003677335	31.790420399233177	21.910788987948887
48	22.5265288378623	26.059550025299494	28.796884242333544	22.617036894504665
49	22.823352503937457	24.125029397301862	30.30879638537903	22.74282171338165
50	21.37415460486463	25.508662404948723	29.729402290495226	23.38778069969142
51	21.723002586962565	24.41828975406039	27.269293538294885	26.589414120682157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	89.0
1	289.0
2	489.0
3	1411.0
4	2333.0
5	1640.0
6	947.0
7	897.5
8	848.0
9	858.5
10	869.0
11	857.0
12	845.0
13	783.0
14	721.0
15	703.0
16	685.0
17	662.0
18	639.0
19	758.0
20	877.0
21	809.0
22	741.0
23	909.5
24	1078.0
25	1261.0
26	1694.0
27	1944.0
28	2494.5
29	3045.0
30	3798.5
31	4552.0
32	5345.0
33	6138.0
34	7168.0
35	8198.0
36	8318.5
37	8439.0
38	9452.0
39	10465.0
40	11681.5
41	12898.0
42	14169.0
43	15440.0
44	16910.0
45	18380.0
46	23603.5
47	28827.0
48	26459.0
49	24091.0
50	23633.0
51	23175.0
52	21135.5
53	19096.0
54	17893.5
55	16691.0
56	15539.0
57	14387.0
58	13804.0
59	13221.0
60	12100.5
61	10980.0
62	9798.0
63	8616.0
64	7413.5
65	6211.0
66	5303.0
67	4395.0
68	3850.5
69	3306.0
70	2707.0
71	2108.0
72	1777.5
73	1447.0
74	1175.5
75	737.5
76	571.0
77	429.5
78	288.0
79	196.0
80	104.0
81	82.5
82	61.0
83	39.0
84	17.0
85	15.5
86	14.0
87	12.5
88	11.0
89	10.0
90	9.0
91	5.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	280638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.83432987011284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.8389781550816	26.10135970611137
2	8.947997316433096	6.412909750278958
3	3.859028481878101	4.148570987933418
4	2.28899966025818	3.2809907559307128
5	1.557999537399103	2.7914934680321326
6	1.1989996404822478	2.577920917871254
7	0.8849997345834246	2.2199360696817316
8	0.8309997507773885	2.3822655353070794
9	0.7059997882657318	2.276912637084964
>10	6.814997956134504	42.386982718009435
>50	0.04799998560446899	1.1861159629762714
>100	0.01699999490158277	1.1413230640723337
>500	0.001999999400186208	0.48591336730991663
>1k	0.003999998800372416	2.607305059400408
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG	2353	0.8384466822026954	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	2097	0.7472259636970047	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	1626	0.5793940948838004	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1200	0.42759711799542466	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	828	0.29504201141684305	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	528	0.18814273191798686	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	379	0.13504942310022164	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	320	0.11402589813211325	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTC	302	0.10761194136218188	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0912207185056906	0.0
2	0.0	0.0	0.0	0.6677641659361883	0.0
3	0.0	0.0	0.0	0.8227681212095297	0.0
4	0.0	0.0	0.0	1.1452476143644126	0.0
5	0.0	0.0	0.0	2.3065301206536533	0.0
6	0.0	0.0	0.0	2.719161339519238	0.0
7	0.0	0.0	0.0	3.17633392484268	0.0
8	0.0	0.0	0.0	3.878662191150165	0.0
9	0.0	0.0	0.0	4.110989958594346	0.0
10	0.0	0.0	0.0	5.576579080523664	0.0
11	0.0	0.0	0.0	6.253964181614749	0.0
12	0.0	0.0	0.0	7.671448627769582	0.0
13	0.0	0.0	0.0	7.959007689621505	0.0
14	0.0	0.0	0.0	8.095126105516716	0.0
15	0.0	0.0	0.0	8.39658207370349	0.0
16	0.0	0.0	0.0	8.799592357414177	0.0
17	0.0	0.0	0.0	9.243580698266094	0.0
18	0.0	0.0	0.0	9.767387167810488	0.0
19	0.0	0.0	0.0	10.088797668170383	0.0
20	0.0	0.0	0.0	10.396311262195427	0.0
21	0.0	0.0	0.0	10.74515924429336	0.0
22	0.0	0.0	0.0	11.168124060177167	0.0
23	0.0	0.0	0.0	11.530868948609953	0.0
24	0.0	0.0	0.0	11.854417434559824	0.0
25	0.0	0.0	0.0	12.135918870573478	0.0
26	0.0	0.0	0.0	12.420983615903761	0.0
27	0.0	0.0	0.0	12.737049152288714	0.0
28	0.0	0.0	0.0	13.025677206935626	0.0
29	3.5633093166285393E-4	0.0	0.0	13.311454614129234	0.0
30	3.5633093166285393E-4	0.0	0.0	13.675981157220333	0.0
31	3.5633093166285393E-4	0.0	0.0	13.978506118202096	0.0
32	3.5633093166285393E-4	0.0	0.0	14.2835253957055	0.0
33	3.5633093166285393E-4	0.0	0.0	14.582843378302297	0.0
34	3.5633093166285393E-4	0.0	0.0	14.895701936302283	0.0
35	3.5633093166285393E-4	0.0	0.0	15.240273947220262	0.0
36	3.5633093166285393E-4	0.0	0.0	15.545649555655329	0.0
37	3.5633093166285393E-4	0.0	0.0	15.865278401356909	0.0
38	3.5633093166285393E-4	0.0	0.0	16.199873146188327	0.0
39	3.5633093166285393E-4	0.0	0.0	16.713702349646162	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	25	3.8832448E-5	45.000004	24
ATCTAGT	25	3.8832448E-5	45.000004	15
GCGTTAG	35	1.2075361E-7	45.000004	1
GCGATTA	25	3.8832448E-5	45.000004	9
TAGGGCG	35	1.2075361E-7	45.000004	5
CGGTCTA	25	3.8832448E-5	45.000004	31
GTACAAC	20	7.024166E-4	45.0	26
CGGCCAA	20	7.024166E-4	45.0	21
GGTATCG	20	7.024166E-4	45.0	8
GTATCGA	20	7.024166E-4	45.0	9
ACACTGC	20	7.024166E-4	45.0	32
CCTGCGT	20	7.024166E-4	45.0	23
CGATAAT	20	7.024166E-4	45.0	12
TACGGCT	240	0.0	43.125004	7
ACACGAC	65	0.0	41.538464	26
CGAATAT	60	3.6379788E-12	41.250004	14
TACGGGT	45	1.9193976E-8	40.0	4
GTCGGGA	85	0.0	39.705883	4
TCAAGCG	75	0.0	39.0	17
GACACGA	70	0.0	38.57143	25
>>END_MODULE