##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936673.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724006
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276283621958935	31.0	31.0	33.0	30.0	34.0
2	31.30125717190189	31.0	31.0	34.0	30.0	34.0
3	31.104564050574165	31.0	31.0	34.0	28.0	34.0
4	35.07602285063936	35.0	35.0	37.0	32.0	37.0
5	35.26076303235056	35.0	35.0	37.0	33.0	37.0
6	35.22635724013337	36.0	35.0	37.0	33.0	37.0
7	30.917446816739087	35.0	35.0	37.0	0.0	37.0
8	32.9756079369508	35.0	35.0	37.0	17.0	37.0
9	36.26491493164421	39.0	35.0	39.0	32.0	39.0
10	36.17229415225841	37.0	35.0	39.0	32.0	39.0
11	36.414562862738705	38.0	35.0	39.0	32.0	39.0
12	36.45196586768618	38.0	35.0	39.0	32.0	39.0
13	36.29457214442974	38.0	35.0	39.0	32.0	39.0
14	37.29208183357596	39.0	36.0	40.0	32.0	41.0
15	37.45247276956268	39.0	36.0	41.0	32.0	41.0
16	37.50853998447527	39.0	36.0	41.0	33.0	41.0
17	37.36901075405452	39.0	36.0	41.0	32.0	41.0
18	37.308599652489065	39.0	36.0	40.0	32.0	41.0
19	37.123581848769206	39.0	35.0	40.0	32.0	41.0
20	37.05528545343547	39.0	35.0	40.0	32.0	41.0
21	37.12520200108839	39.0	35.0	40.0	32.0	41.0
22	37.24107949381635	39.0	35.0	40.0	32.0	41.0
23	37.3179006803811	39.0	35.0	40.0	32.0	41.0
24	37.24723137653555	39.0	35.0	41.0	32.0	41.0
25	36.941622030756655	39.0	35.0	40.0	31.0	41.0
26	36.913572539454094	39.0	35.0	40.0	31.0	41.0
27	36.95125454761425	39.0	35.0	40.0	32.0	41.0
28	36.890021629655	39.0	35.0	40.0	31.0	41.0
29	36.808694955566665	39.0	35.0	40.0	31.0	41.0
30	36.62745198244213	39.0	35.0	40.0	31.0	41.0
31	36.64276124783496	39.0	35.0	40.0	31.0	41.0
32	36.5017707035577	39.0	35.0	40.0	31.0	41.0
33	36.34215462302799	39.0	35.0	40.0	30.0	41.0
34	36.213768946666185	39.0	35.0	40.0	30.0	41.0
35	36.139087245133325	39.0	35.0	40.0	30.0	41.0
36	36.08195097830681	38.0	35.0	40.0	30.0	41.0
37	35.99016030253893	38.0	35.0	40.0	29.0	41.0
38	35.8377623942343	38.0	35.0	40.0	29.0	41.0
39	35.742989698980395	38.0	35.0	40.0	29.0	41.0
40	35.535396115501804	38.0	35.0	40.0	27.0	41.0
41	35.36040032817407	38.0	34.0	40.0	26.0	41.0
42	35.371962387052044	38.0	34.0	40.0	27.0	41.0
43	35.37057013339668	38.0	34.0	40.0	27.0	41.0
44	35.2378516200142	38.0	34.0	40.0	27.0	41.0
45	35.194450045994095	38.0	34.0	40.0	26.0	41.0
46	35.10448670314887	38.0	34.0	40.0	26.0	41.0
47	34.98835783128869	37.0	34.0	40.0	26.0	41.0
48	34.893947011488855	37.0	34.0	40.0	26.0	41.0
49	34.777988027723524	37.0	34.0	40.0	26.0	41.0
50	34.61974210158479	37.0	34.0	40.0	24.0	41.0
51	32.872275920365304	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	39.0
9	55.0
10	88.0
11	52.0
12	38.0
13	34.0
14	41.0
15	53.0
16	87.0
17	184.0
18	386.0
19	719.0
20	1394.0
21	1982.0
22	2942.0
23	3888.0
24	5229.0
25	6924.0
26	8757.0
27	9985.0
28	11470.0
29	12920.0
30	16095.0
31	20712.0
32	27690.0
33	39148.0
34	59393.0
35	62222.0
36	73898.0
37	108279.0
38	138474.0
39	110815.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.80515354845125	19.358264986754254	22.86514200158562	12.97143946320887
2	31.528606116523893	28.27725184597918	23.831708577000743	16.362433460496185
3	31.456645386916684	26.9743620909219	26.126026579890222	15.442965942271197
4	28.76233069891686	23.86195694510819	29.214951257310023	18.160761098664928
5	27.805570672066253	28.21081593246465	24.148694900318507	19.834918495150593
6	25.410010414278332	36.03948033579832	24.484327477949076	14.066181771974264
7	67.27527119940994	17.754548995450314	11.332779010118701	3.6374007950210356
8	78.74437504661563	7.795929867984519	9.416910909578096	4.0427841758217475
9	73.36941959044539	6.573978668685067	10.74631978188026	9.31028195898929
10	44.96316328870203	21.660593972978123	18.363521849266444	15.012720889053405
11	32.84558415261752	24.321207282812573	24.013613146852375	18.819595417717533
12	29.942845777521182	20.713640494692033	28.92075480037458	20.422758927412204
13	26.680441874791093	21.95727659715527	32.21174962638431	19.150531901669325
14	21.11073665135372	26.512211224768855	30.533034256622184	21.844017867255243
15	20.062817158973818	24.51637141128665	35.17926647016738	20.24154495957216
16	24.372726192876854	24.193031549462297	29.898371007975072	21.535871249685776
17	24.90310853777455	23.834470985047084	28.593547567285356	22.66887290989301
18	25.28749761742306	24.05532550835214	28.88470537536982	21.77247149885498
19	25.43501020709773	26.55309486385472	26.191633770990848	21.8202611580567
20	25.945226973257128	25.848542691635153	28.426283760079336	19.779946575028383
21	26.462073518727745	26.535691693162768	28.689403126493428	18.312831661616062
22	24.26361107504634	22.487106460443698	29.95223796487874	23.29704449963122
23	23.146907622312522	25.04868744181678	29.617986591271343	22.186418344599353
24	24.42465946414809	24.629215779979724	27.998939235310203	22.947185520561984
25	24.751452336030365	26.7604135877327	26.32409123681295	22.164042839423985
26	22.093601434242256	24.574658221064468	28.017999850829966	25.313740493863314
27	23.204641950481072	25.226586520001216	28.488023579915083	23.080747949602628
28	21.437943884442948	24.273003262403904	30.96977096874888	23.319281884404273
29	22.905611279464537	24.23350082734121	29.677654605072334	23.183233288121922
30	23.06182545448518	24.460018287141267	31.451673052433264	21.026483205940284
31	24.529216608702136	23.784057038201343	29.183736046386354	22.502990306710167
32	26.04356869970691	25.48666723756433	26.559586522763624	21.91017753996514
33	26.649641025074377	23.078538023165553	26.258069684505376	24.01375126725469
34	23.782123352568902	23.85242663734831	29.413430275439705	22.952019734643084
35	22.708927826564974	26.5642826164424	27.510821733521546	23.215967823471075
36	25.577274221484352	25.982105120675797	28.10515382469206	20.33546683314779
37	23.684057866923755	26.385278575039433	27.88554238500786	22.045121173028953
38	24.38239462103905	26.06249119482435	26.670359085421946	22.884755098714653
39	24.127976839970938	23.980602370698588	26.52022220810325	25.371198581227226
40	25.890807534744187	24.24164993107792	28.023386546520335	21.844155987657558
41	22.72577851564766	24.738054657005605	27.445352662823236	25.0908141645235
42	21.86763645605147	25.411115377496873	30.19726355858929	22.523984607862367
43	24.301041704074276	23.20229390364168	28.074214854573025	24.422449537711014
44	23.482125838736142	23.634887003698864	28.39741659599506	24.48557056156993
45	22.166805247470325	23.219697074333638	27.361927939823705	27.251569738372332
46	24.1623688201479	25.33860216628039	27.082924727143144	23.416104286428567
47	21.887940155192084	24.177838305207416	31.24090131849736	22.69332022110314
48	22.33475965668793	25.299652212826967	28.58595094515791	23.779637185327193
49	22.7745350176656	23.500219611439686	30.129170200246957	23.596075170647758
50	21.66459946464532	23.903116824998687	30.003756874943026	24.428526835412967
51	21.625235149984945	23.90435990861954	28.438300235080927	26.03210470631459
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	272.0
1	871.5
2	1471.0
3	3623.5
4	5776.0
5	4019.0
6	2262.0
7	2191.5
8	2121.0
9	2071.5
10	2022.0
11	1941.5
12	1861.0
13	1900.0
14	1939.0
15	1861.0
16	1783.0
17	1676.0
18	1569.0
19	1696.5
20	1824.0
21	1959.0
22	2094.0
23	2263.5
24	2433.0
25	3137.0
26	4580.5
27	5320.0
28	6208.0
29	7096.0
30	8922.0
31	10748.0
32	12264.0
33	13780.0
34	16450.0
35	19120.0
36	20227.5
37	21335.0
38	22941.0
39	24547.0
40	27069.0
41	29591.0
42	32872.5
43	36154.0
44	38871.5
45	41589.0
46	59894.5
47	78200.0
48	66819.5
49	55439.0
50	55159.0
51	54879.0
52	49820.5
53	44762.0
54	42612.0
55	40462.0
56	40428.0
57	40394.0
58	39770.0
59	39146.0
60	37124.0
61	35102.0
62	31884.5
63	28667.0
64	25812.5
65	22958.0
66	19342.5
67	15727.0
68	13276.5
69	10826.0
70	9126.5
71	7427.0
72	5817.5
73	4208.0
74	3256.5
75	1889.0
76	1473.0
77	1109.0
78	745.0
79	551.5
80	358.0
81	277.0
82	196.0
83	152.5
84	109.0
85	70.0
86	31.0
87	29.0
88	27.0
89	17.0
90	7.0
91	6.0
92	5.0
93	5.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	724006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.53004486777348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.108923928432	21.589098038412352
2	8.464540098995087	4.999164978167855
3	3.699584007993225	3.277466452444115
4	2.3188140730966635	2.738987344742762
5	1.5863086068781291	2.3421882167623203
6	1.1709531998174298	2.0746980317202977
7	0.8779744682569439	1.8148637808270984
8	0.7147616444313012	1.6885554743855904
9	0.5297654068904991	1.4079596611383645
>10	7.330134344777041	48.581549645408586
>50	0.1601706699075496	2.903029324104295
>100	0.03156987116634192	1.8113257816325428
>500	0.00232131405634867	0.41992816145477435
>1k	0.0027855768676184043	1.6150028540441532
>5k	0.0013927884338092022	2.7361822547549415
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTC	7395	1.0214003751350127	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCG	6380	0.8812081667831482	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGC	6183	0.853998447526678	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT	3252	0.4491675483352348	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2877	0.3973723974663193	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	1894	0.2616000419886023	No Hit
GAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT	1489	0.20566127905017362	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT	1157	0.1598053054808938	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTC	1111	0.15345176697430685	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTA	737	0.10179473650770851	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.762408046342157E-4	0.0	0.0	0.06560719110062624	0.0
2	2.762408046342157E-4	0.0	0.0	0.5042775888597608	0.0
3	2.762408046342157E-4	0.0	0.0	0.6392212219235752	0.0
4	2.762408046342157E-4	0.0	0.0	1.0051021676615939	0.0
5	2.762408046342157E-4	0.0	0.0	2.5140675629759976	0.0
6	2.762408046342157E-4	0.0	0.0	3.336850799579009	0.0
7	2.762408046342157E-4	0.0	0.0	3.752869451358138	0.0
8	2.762408046342157E-4	0.0	0.0	4.3158482112026695	0.0
9	2.762408046342157E-4	0.0	0.0	4.477863443120637	0.0
10	2.762408046342157E-4	0.0	0.0	5.813211492722436	0.0
11	2.762408046342157E-4	0.0	0.0	6.339035864343666	0.0
12	2.762408046342157E-4	0.0	0.0	7.7496595332082885	0.0
13	2.762408046342157E-4	0.0	0.0	8.047861481810925	0.0
14	2.762408046342157E-4	0.0	0.0	8.21761145625865	0.0
15	2.762408046342157E-4	0.0	0.0	8.487913083593229	0.0
16	2.762408046342157E-4	0.0	0.0	8.800893915243796	0.0
17	2.762408046342157E-4	0.0	0.0	9.131830399195588	0.0
18	2.762408046342157E-4	0.0	0.0	9.51566699723483	0.0
19	2.762408046342157E-4	0.0	0.0	9.791907801869046	0.0
20	2.762408046342157E-4	0.0	0.0	10.055027168283136	0.0
21	2.762408046342157E-4	0.0	0.0	10.328367444468693	0.0
22	2.762408046342157E-4	0.0	0.0	10.643143841349382	0.0
23	2.762408046342157E-4	0.0	0.0	10.910959301442253	0.0
24	4.143612069513236E-4	0.0	0.0	11.16040474802695	0.0
25	4.143612069513236E-4	0.0	0.0	11.366756629088709	0.0
26	4.143612069513236E-4	0.0	0.0	11.599903868199988	0.0
27	4.143612069513236E-4	0.0	0.0	11.868962411913714	0.0
28	4.143612069513236E-4	0.0	0.0	12.093822426885964	0.0
29	4.143612069513236E-4	0.0	0.0	12.34147230824054	0.0
30	4.143612069513236E-4	0.0	0.0	12.637326210003785	0.0
31	4.143612069513236E-4	0.0	0.0	12.884976091358359	0.0
32	4.143612069513236E-4	0.0	0.0	13.117294608055735	0.0
33	4.143612069513236E-4	0.0	0.0	13.367983138261286	0.0
34	4.143612069513236E-4	0.0	0.0	13.649886879390502	0.0
35	4.143612069513236E-4	0.0	0.0	13.954856727706677	0.0
36	4.143612069513236E-4	0.0	0.0	14.21590428808601	0.0
37	5.524816092684315E-4	0.0	0.0	14.488277721455347	0.0
38	5.524816092684315E-4	0.0	0.0	14.805402165175426	0.0
39	5.524816092684315E-4	0.0	0.0	15.335232028463853	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCAT	25	3.889371E-5	45.0	40
GCACGTA	20	7.031555E-4	45.0	45
TCGACGT	25	3.889371E-5	45.0	26
TTCGACG	25	3.889371E-5	45.0	25
TATCGCG	25	3.889371E-5	45.0	1
TACGGCT	795	0.0	40.18868	7
ACTACGG	45	1.9266736E-8	40.0	2
CGGGTAC	40	3.4558616E-7	39.375	6
ATACCGG	40	3.4558616E-7	39.375	2
GTTTACG	35	6.2450854E-6	38.57143	1
ATTCGGC	35	6.2450854E-6	38.57143	17
ACGGCTG	915	0.0	38.114754	8
TTTACGG	65	9.094947E-12	38.07692	2
CACGACC	30	1.1395043E-4	37.500004	27
GCCGATA	30	1.1395043E-4	37.500004	9
TACTACG	30	1.1395043E-4	37.500004	1
ACGACCA	30	1.1395043E-4	37.500004	28
CGTTTTT	1440	0.0	37.1875	1
CGGTCTA	55	2.746674E-9	36.81818	31
TTACGGG	160	0.0	36.5625	3
>>END_MODULE