##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936670.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	694679
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15087400079749	31.0	31.0	33.0	28.0	34.0
2	31.182333135160267	31.0	31.0	34.0	28.0	34.0
3	31.018589881081766	31.0	31.0	34.0	28.0	34.0
4	34.97184454978486	35.0	35.0	37.0	32.0	37.0
5	35.17387887067264	35.0	35.0	37.0	33.0	37.0
6	35.11808619520671	36.0	35.0	37.0	32.0	37.0
7	30.950225931689314	35.0	35.0	37.0	0.0	37.0
8	32.963499688345266	35.0	35.0	37.0	17.0	37.0
9	36.21419389387041	39.0	35.0	39.0	32.0	39.0
10	36.04449393172962	37.0	35.0	39.0	32.0	39.0
11	36.24630656749376	37.0	35.0	39.0	32.0	39.0
12	36.28226130342215	38.0	35.0	39.0	32.0	39.0
13	36.174564079236596	38.0	35.0	39.0	32.0	39.0
14	37.117133237077844	39.0	36.0	40.0	31.0	41.0
15	37.28636679675073	39.0	36.0	41.0	32.0	41.0
16	37.33432995671382	39.0	36.0	40.0	32.0	41.0
17	37.230385544978326	39.0	36.0	40.0	32.0	41.0
18	37.173177827456996	39.0	36.0	40.0	32.0	41.0
19	37.0102407011008	39.0	35.0	40.0	31.0	41.0
20	36.92395624453884	39.0	35.0	40.0	31.0	41.0
21	36.967613818756575	39.0	35.0	40.0	31.0	41.0
22	37.09346187231801	39.0	35.0	40.0	32.0	41.0
23	37.17298637212295	39.0	35.0	40.0	32.0	41.0
24	37.09471568882894	39.0	35.0	40.0	32.0	41.0
25	36.823650923664026	39.0	35.0	40.0	31.0	41.0
26	36.7701758653997	39.0	35.0	40.0	31.0	41.0
27	36.79347583560177	39.0	35.0	40.0	31.0	41.0
28	36.76892348840256	39.0	35.0	40.0	31.0	41.0
29	36.700568176092844	39.0	35.0	40.0	31.0	41.0
30	36.4768605355855	38.0	35.0	40.0	30.0	41.0
31	36.476611499699864	39.0	35.0	40.0	30.0	41.0
32	36.32722739567484	38.0	35.0	40.0	30.0	41.0
33	36.21022515435187	38.0	35.0	40.0	30.0	41.0
34	36.091607778556714	38.0	35.0	40.0	30.0	41.0
35	36.00437180337969	38.0	35.0	40.0	29.0	41.0
36	35.98123881677724	38.0	35.0	40.0	29.0	41.0
37	35.85958838542694	38.0	35.0	40.0	29.0	41.0
38	35.761818048335996	38.0	35.0	40.0	29.0	41.0
39	35.685448962758336	38.0	35.0	40.0	28.0	41.0
40	35.57799501640326	38.0	35.0	40.0	27.0	41.0
41	35.37049198262795	38.0	34.0	40.0	27.0	41.0
42	35.39665802478555	38.0	34.0	40.0	27.0	41.0
43	35.39063797811651	38.0	34.0	40.0	27.0	41.0
44	35.23730960630737	38.0	34.0	40.0	27.0	41.0
45	35.177025647817196	38.0	34.0	40.0	26.0	41.0
46	35.10909787110305	38.0	34.0	40.0	26.0	41.0
47	35.01291963626365	37.0	34.0	40.0	26.0	41.0
48	34.922187082091156	37.0	34.0	40.0	26.0	41.0
49	34.825666242969774	37.0	34.0	40.0	26.0	41.0
50	34.65277919729832	37.0	34.0	40.0	25.0	41.0
51	32.97015312108182	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	19.0
9	61.0
10	88.0
11	60.0
12	45.0
13	38.0
14	41.0
15	70.0
16	127.0
17	237.0
18	528.0
19	852.0
20	1310.0
21	1954.0
22	2905.0
23	3867.0
24	5204.0
25	7120.0
26	8408.0
27	9941.0
28	11158.0
29	13306.0
30	16776.0
31	21241.0
32	27747.0
33	38555.0
34	55941.0
35	59407.0
36	70297.0
37	101708.0
38	130998.0
39	104665.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.034037303560346	19.74192396776065	22.23789692793362	12.98614180074538
2	31.75797742554475	26.4087441825649	24.610935410455763	17.22234298143459
3	32.48334842423623	24.959297747592775	26.23197188917471	16.325381938996284
4	28.29220402516846	24.529746832709783	28.059290693975203	19.118758448146554
5	27.44145137538345	28.32718421026114	24.891784550850105	19.339579863505303
6	26.134948659740687	35.74514272059469	24.801527036228244	13.318381583436379
7	68.44211499124056	17.764895728818633	10.087968687696042	3.7050205922447637
8	79.05968080221224	7.612724726096514	8.914908900369811	4.412685571321431
9	73.3757606030987	7.345694918084468	10.346793267106102	8.931751211710733
10	45.11436217303243	21.604366909032805	18.545688008418278	14.73558290951648
11	32.573749890237075	24.097028987489185	24.260701705391988	19.068519416881756
12	29.33210878693612	22.226956623130974	27.8964816843463	20.54445290558661
13	26.005248467277692	22.481318709792582	32.049766870741735	19.463665952187988
14	21.385416861600824	26.547081457766826	30.46990048641171	21.59760119422064
15	19.522829968949686	25.016158542290757	35.38310500245437	20.077906486305185
16	24.32792699937669	25.091013259361517	28.98892869944248	21.592131041819314
17	25.13995672821548	24.38003739856826	28.49848635125	21.981519521966263
18	24.890632939818246	24.7046477581732	29.236237168533957	21.168482133474598
19	25.47421183021223	25.933272777786577	26.911710300728824	21.680805091272372
20	25.411736931733937	26.712049738080466	28.815179385010918	19.061033945174678
21	25.44139091580428	26.44444412455249	29.65614334102514	18.458021618618094
22	24.419624027788377	22.537459747595655	29.941454974167925	23.10146125044805
23	23.59118384174561	25.453770734396752	30.313281386079037	20.6417640377786
24	23.431973616591257	24.814482660336644	28.961002131919923	22.792541591152172
25	23.583698370038537	27.856175298231268	26.722270285988202	21.83785604574199
26	22.178013154277014	25.36120999771117	27.8980651495151	24.562711698496717
27	22.613034221561325	26.42198770943126	28.55174836147343	22.413229707533983
28	21.44674014904726	25.66825828908028	29.854508341262655	23.030493220609806
29	24.00907469493104	25.536110923174586	28.23232025151185	22.222494130382522
30	23.646749074032755	25.467590066779046	29.648657869318058	21.237002989870142
31	25.726702548947067	25.06855684424029	26.883207927690343	22.3215326791223
32	26.54880887431461	26.748757339720935	26.0700265878197	20.632407198144755
33	25.34407978361229	24.890345037060282	26.157261123482932	23.6083140558445
34	24.484546099709362	25.732892458243306	28.365187374312452	21.417374067734883
35	23.08605845289695	26.410327647733702	28.853470451820197	21.650143447549155
36	27.894610316419527	25.850932589008735	25.95803241497152	20.296424679600218
37	23.13557772726684	28.334669681968215	28.22195575222513	20.307796838539815
38	24.403213570584402	26.66109094992076	26.37203658092443	22.56365889857042
39	23.645453511621913	26.432352208717987	27.019817786344486	22.902376493315618
40	23.7963145567953	24.066799197902917	29.795920130016885	22.340966115284903
41	23.10736325698632	25.678766739745985	27.8186039883169	23.395266014950792
42	22.46274898190387	24.82038466687492	30.6871231172959	22.02974323392531
43	23.82093024260126	24.3618995248165	27.962267464541174	23.854902768041068
44	22.816437520063225	24.86817652469702	28.98864079668451	23.326745158555244
45	22.114530596145844	24.960737261382597	27.524655272435183	25.400076870036376
46	23.814020576410112	26.083989871580975	27.746340396067826	22.35564915594109
47	21.04497185030784	25.368119663902323	31.075791840547932	22.511116645241902
48	21.927825657605887	26.805906037176886	28.564272131444884	22.701996173772347
49	22.867684210980897	24.711989278501292	29.799374963112456	22.620951547405348
50	22.06328390522817	25.251231144168745	28.98921660220044	23.696268348402644
51	21.83598467781522	25.276134732732675	27.610450294308592	25.27743029514351
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	171.0
1	732.0
2	1293.0
3	2974.5
4	4656.0
5	3243.0
6	1830.0
7	1763.0
8	1696.0
9	1633.5
10	1571.0
11	1599.5
12	1628.0
13	1638.5
14	1649.0
15	1709.5
16	1770.0
17	1630.5
18	1491.0
19	1623.5
20	1756.0
21	2100.0
22	2444.0
23	2842.5
24	3241.0
25	3482.0
26	4690.5
27	5658.0
28	7007.0
29	8356.0
30	10151.5
31	11947.0
32	13495.0
33	15043.0
34	17905.0
35	20767.0
36	21619.0
37	22471.0
38	24813.5
39	27156.0
40	29326.0
41	31496.0
42	34527.0
43	37558.0
44	40146.5
45	42735.0
46	55369.5
47	68004.0
48	61650.0
49	55296.0
50	53951.0
51	52606.0
52	47646.0
53	42686.0
54	40708.0
55	38730.0
56	38018.0
57	37306.0
58	35487.0
59	33668.0
60	30900.5
61	28133.0
62	26741.0
63	25349.0
64	22699.0
65	20049.0
66	17286.5
67	14524.0
68	12195.5
69	9867.0
70	8402.0
71	6937.0
72	5581.0
73	4225.0
74	3293.0
75	1943.5
76	1526.0
77	1012.5
78	499.0
79	415.5
80	332.0
81	277.5
82	223.0
83	174.5
84	126.0
85	96.5
86	67.0
87	44.0
88	21.0
89	16.5
90	12.0
91	14.0
92	16.0
93	9.5
94	3.0
95	4.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	694679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.997326123740887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.1413102888398	22.67185048140892
2	8.514048929747009	5.278255026177103
3	3.799940078707885	3.533639456111457
4	2.359149422077208	2.9250929604304825
5	1.5721120198150467	2.4365634490629997
6	1.1579880157538949	2.1536719303024245
7	0.8445074257132865	1.8324230462128936
8	0.6502007881285962	1.612358870042836
9	0.5546106726863999	1.5472303103670884
>10	7.277644741246035	49.15467747956014
>50	0.09844554849862454	1.8829345311116155
>100	0.024495840702623003	1.4741989136971871
>500	0.0027731140418063775	0.5669003985908426
>1k	0.001848742694537585	1.462021371650126
>5k	9.243713472687925E-4	1.4681817752739335
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGC	5187	0.7466758027808528	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCG	5061	0.7285379290290911	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTC	4947	0.7121274718251164	RNA PCR Primer, Index 41 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2382	0.34289218473568367	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	1633	0.2350726018779897	Illumina PCR Primer Index 3 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCT	1243	0.17893156407491806	TruSeq Adapter, Index 11 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCT	927	0.13344292831653182	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTC	851	0.12250262351388196	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05973982227762751	0.0
2	0.0	0.0	0.0	0.48122945993761146	0.0
3	0.0	0.0	0.0	0.592503875890879	0.0
4	0.0	0.0	0.0	0.9320851789099713	0.0
5	0.0	0.0	0.0	2.087294995242407	0.0
6	0.0	0.0	0.0	2.51324712565084	0.0
7	0.0	0.0	0.0	2.9517230260307277	0.0
8	0.0	0.0	0.0	3.6020953562724656	0.0
9	0.0	0.0	0.0	3.8331373195389524	0.0
10	0.0	0.0	0.0	5.089976809432846	0.0
11	1.4395137898223496E-4	0.0	0.0	5.651531138842545	0.0
12	1.4395137898223496E-4	0.0	0.0	6.949684674504339	0.0
13	1.4395137898223496E-4	0.0	0.0	7.16287666677703	1.4395137898223496E-4
14	2.879027579644699E-4	0.0	0.0	7.2829321168482135	1.4395137898223496E-4
15	2.879027579644699E-4	0.0	0.0	7.510807149777091	1.4395137898223496E-4
16	2.879027579644699E-4	0.0	0.0	7.810082066681158	1.4395137898223496E-4
17	2.879027579644699E-4	0.0	0.0	8.14577668246773	1.4395137898223496E-4
18	2.879027579644699E-4	0.0	0.0	8.50522327578637	1.4395137898223496E-4
19	2.879027579644699E-4	0.0	0.0	8.788375638244426	1.4395137898223496E-4
20	2.879027579644699E-4	0.0	0.0	9.04173006525316	1.4395137898223496E-4
21	2.879027579644699E-4	0.0	0.0	9.347482794211427	1.4395137898223496E-4
22	2.879027579644699E-4	0.0	0.0	9.67094154278451	1.4395137898223496E-4
23	2.879027579644699E-4	0.0	0.0	9.953230196968672	1.4395137898223496E-4
24	2.879027579644699E-4	0.0	0.0	10.205289061566566	1.4395137898223496E-4
25	2.879027579644699E-4	0.0	0.0	10.438634246896768	1.4395137898223496E-4
26	2.879027579644699E-4	0.0	0.0	10.64995487124269	1.4395137898223496E-4
27	2.879027579644699E-4	0.0	0.0	10.915545165464913	1.4395137898223496E-4
28	2.879027579644699E-4	0.0	0.0	11.148170593900204	1.4395137898223496E-4
29	4.3185413694670486E-4	0.0	0.0	11.433050372906047	1.4395137898223496E-4
30	4.3185413694670486E-4	0.0	0.0	11.763850641807224	1.4395137898223496E-4
31	4.3185413694670486E-4	0.0	0.0	12.040237289453115	1.4395137898223496E-4
32	4.3185413694670486E-4	0.0	0.0	12.29690259817844	1.4395137898223496E-4
33	4.3185413694670486E-4	0.0	0.0	12.549681219671244	1.4395137898223496E-4
34	4.3185413694670486E-4	0.0	0.0	12.818294492852095	1.4395137898223496E-4
35	4.3185413694670486E-4	0.0	0.0	13.139018165224513	1.4395137898223496E-4
36	4.3185413694670486E-4	0.0	0.0	13.420443111134784	1.4395137898223496E-4
37	4.3185413694670486E-4	0.0	0.0	13.711224896678898	1.4395137898223496E-4
38	4.3185413694670486E-4	0.0	0.0	14.0888093637493	1.4395137898223496E-4
39	4.3185413694670486E-4	0.0	0.0	14.749258290519794	1.4395137898223496E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAGG	25	3.889206E-5	45.0	2
GATGCGT	25	3.889206E-5	45.0	11
ATAACGC	20	7.031357E-4	45.0	11
TAGGACG	25	3.889206E-5	45.0	1
TATCGTG	25	3.889206E-5	45.0	28
TCGAGGG	135	0.0	41.666664	3
CGTTTTT	1155	0.0	40.51948	1
TACGGCT	680	0.0	39.705883	7
TACGAAT	40	3.4555887E-7	39.375	12
CGGTCTA	40	3.4555887E-7	39.375	31
CATGACG	35	6.2447198E-6	38.57143	1
TCGACCG	35	6.2447198E-6	38.57143	24
CGAAATA	30	1.1394569E-4	37.500004	37
TAGCACG	30	1.1394569E-4	37.500004	1
CTCGCGA	30	1.1394569E-4	37.500004	14
CACAACG	85	0.0	37.058823	12
CTTCGAA	25	0.0021065357	36.0	27
CTCACGA	50	4.874164E-8	36.0	24
TTCACGC	25	0.0021065357	36.0	34
TACGGAC	25	0.0021065357	36.0	43
>>END_MODULE