##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936669.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501568
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14151221768534	31.0	31.0	33.0	28.0	34.0
2	31.17701089383693	31.0	31.0	34.0	28.0	34.0
3	30.975118029858365	31.0	31.0	34.0	28.0	34.0
4	34.955038997703205	35.0	35.0	37.0	32.0	37.0
5	35.16976960252648	35.0	35.0	37.0	33.0	37.0
6	35.131407904810516	36.0	35.0	37.0	32.0	37.0
7	30.991674109991067	35.0	35.0	37.0	0.0	37.0
8	32.98419556271533	35.0	35.0	37.0	17.0	37.0
9	36.1684198353962	39.0	35.0	39.0	32.0	39.0
10	36.09068959742248	37.0	35.0	39.0	32.0	39.0
11	36.34324956935052	37.0	35.0	39.0	32.0	39.0
12	36.38520798775041	38.0	35.0	39.0	32.0	39.0
13	36.2261627536047	38.0	35.0	39.0	32.0	39.0
14	37.22451791182851	39.0	36.0	40.0	32.0	41.0
15	37.36820530815363	39.0	36.0	41.0	32.0	41.0
16	37.39922403343116	39.0	36.0	40.0	32.0	41.0
17	37.2583976649228	39.0	36.0	40.0	32.0	41.0
18	37.16826831057803	39.0	36.0	40.0	32.0	41.0
19	36.98670369720556	39.0	35.0	40.0	31.0	41.0
20	36.910871506954194	39.0	35.0	40.0	31.0	41.0
21	36.98341600740079	39.0	35.0	40.0	31.0	41.0
22	37.116452804006634	39.0	35.0	40.0	32.0	41.0
23	37.18688592573689	39.0	35.0	40.0	32.0	41.0
24	37.08935179277785	39.0	35.0	40.0	32.0	41.0
25	36.835882671940794	39.0	35.0	40.0	31.0	41.0
26	36.75947827612607	39.0	35.0	40.0	31.0	41.0
27	36.811658638509634	39.0	35.0	40.0	31.0	41.0
28	36.765573162562205	39.0	35.0	40.0	31.0	41.0
29	36.72846553209136	39.0	35.0	40.0	31.0	41.0
30	36.51151987367615	38.0	35.0	40.0	30.0	41.0
31	36.50523159372209	39.0	35.0	40.0	30.0	41.0
32	36.360176486538215	38.0	35.0	40.0	30.0	41.0
33	36.25538511228787	38.0	35.0	40.0	30.0	41.0
34	36.18303799285441	38.0	35.0	40.0	30.0	41.0
35	36.136262680234786	39.0	35.0	40.0	30.0	41.0
36	36.07088370868955	38.0	35.0	40.0	30.0	41.0
37	35.98026588618094	38.0	35.0	40.0	29.0	41.0
38	35.84897959997448	38.0	35.0	40.0	29.0	41.0
39	35.76024985645017	38.0	35.0	40.0	29.0	41.0
40	35.656804261834885	38.0	35.0	40.0	28.0	41.0
41	35.46192938943473	38.0	34.0	40.0	27.0	41.0
42	35.49999601250479	38.0	34.0	40.0	27.0	41.0
43	35.467838857343374	38.0	34.0	40.0	27.0	41.0
44	35.2711257496491	38.0	34.0	40.0	27.0	41.0
45	35.2080156628812	38.0	34.0	40.0	26.0	41.0
46	35.10690674046191	38.0	34.0	40.0	26.0	41.0
47	35.013399977670026	37.0	34.0	40.0	26.0	41.0
48	34.94652569541917	37.0	34.0	40.0	26.0	41.0
49	34.87291852749777	37.0	34.0	40.0	26.0	41.0
50	34.71869417506699	37.0	34.0	40.0	25.0	41.0
51	32.923346385734334	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	47.0
10	42.0
11	36.0
12	21.0
13	18.0
14	21.0
15	40.0
16	77.0
17	139.0
18	274.0
19	532.0
20	860.0
21	1323.0
22	1886.0
23	2700.0
24	3576.0
25	4942.0
26	6197.0
27	7075.0
28	8085.0
29	9486.0
30	11630.0
31	15181.0
32	19934.0
33	27610.0
34	40932.0
35	43797.0
36	51541.0
37	74138.0
38	95189.0
39	74221.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.94591361490366	19.023143422227893	21.47884235038918	13.552100612479265
2	31.669285121857854	27.05455690953171	23.913606928671687	17.362551039938754
3	31.753820020415972	26.69468546637744	26.21578729105525	15.335707222151335
4	28.553057611330868	23.621124154651014	29.384450363659564	18.441367870358555
5	27.921438369273954	27.892130279443666	24.572141763429883	19.614289587852497
6	25.479496299604442	36.39287195355366	24.38054261834886	13.747089128493045
7	66.57003636595637	17.644068202118156	12.15169229296925	3.634203138956233
8	76.35335587597295	8.433353004976395	10.403973140232232	4.8093179788184255
9	70.51885287737655	7.252057547530944	11.984815618221258	10.24427395687125
10	41.13759250988899	24.564166772999872	19.004202819956618	15.294037897154523
11	32.489313512823784	24.515319956616054	24.3382751690698	18.657091361490366
12	29.005638318234016	22.058424779890267	28.528135766237078	20.407801135638636
13	25.33076272808473	23.434908128110248	32.051885287737655	19.182443856067373
14	20.885901811917826	27.189932372081156	29.489520862574963	22.434644953426055
15	19.408335459997446	24.967103164476203	35.33199885160138	20.29256252392497
16	22.894004402194717	25.624840500191397	29.43309780528263	22.04805729233125
17	24.099623580451706	24.920250095699885	29.060267002679595	21.919859321168815
18	24.913870103355876	24.598857981370422	29.279778614265663	21.207493301008036
19	25.047650567819318	26.758485389817533	26.137632384841137	22.05623165752201
20	25.921310769427073	26.16554485134618	29.528598315682018	18.384546063544725
21	24.63793543447748	28.614465037641956	28.326368508357792	18.42123101952278
22	23.162362830164604	23.484951193058567	29.405185338777596	23.947500637999234
23	22.275344519586575	27.40406086512696	29.46858651269618	20.85200810259028
24	23.197452788056655	24.607829845604183	28.782139211432945	23.412578154906214
25	22.77876579048105	28.513980158223813	26.122081153502613	22.58517289779252
26	21.150870868954957	25.523159372208752	28.94183839479393	24.384131364042364
27	23.890080706903152	25.801287163455406	27.729639849432182	22.578992280209263
28	19.322604312874823	26.072835587597293	31.265351856577773	23.339208242950107
29	22.89520065075922	25.975341329590407	29.134235038917954	21.995222980732425
30	24.04878301646038	25.20376100548679	29.303504210794944	21.44395176725788
31	24.581313002424395	24.616004210794948	28.780344838586192	22.022337948194462
32	25.067986793415848	27.39030400663519	26.990358236570117	20.551350963378844
33	23.70765280081664	23.835252647696823	26.61872368253158	25.838370868954957
34	21.746004529794565	24.442548168942196	32.67154204414955	21.139905257113693
35	22.160704032155163	25.969559461528647	26.99773510271788	24.872001403598315
36	22.953816830419804	25.974743205308155	28.512783909659305	22.558656054612737
37	22.7773701671558	27.214455467653437	28.1931861681766	21.814988197014163
38	21.33868189358173	26.946296414444305	26.67335236697716	25.041669324996814
39	22.54868731657522	24.72825220109736	28.557244481306622	24.165816001020797
40	23.6817340819191	23.48614744162307	28.287091680489983	24.545026795967846
41	20.280600038279953	25.151126068648715	28.65972310833227	25.90855078473906
42	21.627376547148145	24.01369305856833	30.095022010973587	24.26390838330994
43	23.551342988388413	23.248093977287226	27.573130662243205	25.627432372081156
44	22.849743205308155	24.099822955212456	28.811247926502485	24.239185912976904
45	22.038287929054484	23.33861011866786	27.538638828633406	27.084463123644255
46	23.436503126196246	25.575794309046827	26.830260303687638	24.15744226106929
47	20.156987686614777	24.28444398366722	32.700650759219094	22.857917570498916
48	22.38898813321424	25.535520607375272	28.09349878780145	23.981992471609033
49	21.802626961847647	23.424939390072733	30.815562396325124	23.956871251754496
50	20.33144060227128	24.955938177874188	29.3746810003828	25.33794021947174
51	21.075706584152098	23.574271085874695	27.65926055888733	27.690761771085874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	156.0
1	554.0
2	952.0
3	2630.0
4	4308.0
5	2960.5
6	1613.0
7	1538.0
8	1463.0
9	1444.0
10	1425.0
11	1394.0
12	1363.0
13	1348.0
14	1333.0
15	1425.0
16	1517.0
17	1451.0
18	1385.0
19	1434.0
20	1483.0
21	1561.0
22	1639.0
23	1863.5
24	2088.0
25	2494.5
26	3434.0
27	3967.0
28	4757.0
29	5547.0
30	6495.0
31	7443.0
32	8176.5
33	8910.0
34	10412.0
35	11914.0
36	12879.0
37	13844.0
38	15628.0
39	17412.0
40	19957.0
41	22502.0
42	24102.5
43	25703.0
44	28436.0
45	31169.0
46	44847.5
47	58526.0
48	50988.0
49	43450.0
50	42867.0
51	42284.0
52	37863.0
53	33442.0
54	30651.0
55	27860.0
56	26637.0
57	25414.0
58	24366.5
59	23319.0
60	22163.0
61	21007.0
62	19290.5
63	17574.0
64	14697.5
65	11821.0
66	10263.5
67	8706.0
68	7480.0
69	6254.0
70	5208.5
71	4163.0
72	3194.5
73	2226.0
74	1975.5
75	1332.0
76	939.0
77	673.0
78	407.0
79	301.5
80	196.0
81	140.5
82	85.0
83	74.5
84	64.0
85	44.0
86	24.0
87	18.5
88	13.0
89	18.5
90	24.0
91	13.5
92	3.0
93	1.5
94	0.0
95	2.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	501568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.604901096233284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.13161754001608	24.911856755360333
2	8.84442913011799	5.944323323405192
3	3.7598742423346585	3.790506061437938
4	2.1911435563068844	2.9453264998936692
5	1.4760032973442405	2.480047241248371
6	1.0288290910930178	2.074421991066507
7	0.8131973374979072	1.912919126883619
8	0.6091566288030388	1.637651861043281
9	0.49621079300150894	1.5007603159529277
>10	6.525179912091852	43.899408505285884
>50	0.08172128120096836	1.8059483925985715
>100	0.03434662543229104	1.7446555986679535
>500	0.004145282379759264	0.8557110840962929
>1k	0.002368732788433865	1.3106309767112059
>5k	0.0017765495913253987	3.1858322663482688
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG	5867	1.1697317213219343	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC	5087	1.0142194079367106	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	5055	1.0078394155927013	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2211	0.4408175960188848	No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	2134	0.42546573944111266	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT	1172	0.23366721959933648	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	1069	0.2131316192420569	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTC	823	0.1640854280974863	No Hit
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	659	0.1313879673344392	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTA	615	0.12261547786142657	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT	588	0.11723235932116882	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG	553	0.11025424269490876	No Hit
GAATCTATCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	546	0.10885861936965675	No Hit
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	516	0.10287737654714815	TruSeq Adapter, Index 20 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11603611075666709	0.0
2	0.0	0.0	0.0	0.8738595763685083	0.0
3	0.0	0.0	0.0	1.0724368380757943	0.0
4	0.0	0.0	0.0	1.5453537705754754	0.0
5	0.0	0.0	0.0	3.269546701543958	0.0
6	0.0	0.0	0.0	3.8806303432435882	0.0
7	0.0	0.0	0.0	4.546940793671047	0.0
8	0.0	0.0	0.0	5.483404044915146	0.0
9	0.0	0.0	0.0	5.777282442261069	0.0
10	0.0	0.0	0.0	7.690283271660074	0.0
11	0.0	0.0	0.0	8.501539173152992	0.0
12	0.0	0.0	0.0	10.295712645144826	0.0
13	0.0	0.0	0.0	10.616506635192037	0.0
14	0.0	0.0	0.0	10.785177682786781	0.0
15	0.0	0.0	0.0	11.141859129769044	0.0
16	0.0	0.0	0.0	11.594838586193697	0.0
17	0.0	0.0	0.0	12.065163646803624	0.0
18	0.0	0.0	0.0	12.602279252264898	0.0
19	0.0	0.0	0.0	12.95816320020416	0.0
20	0.0	0.0	0.0	13.308065905320914	0.0
21	0.0	0.0	0.0	13.690865445961466	0.0
22	0.0	0.0	0.0	14.12550242439709	0.0
23	0.0	0.0	0.0	14.5144825826209	0.0
24	0.0	0.0	0.0	14.849631555442134	0.0
25	0.0	0.0	0.0	15.143509952788056	0.0
26	0.0	0.0	0.0	15.431207732550721	0.0
27	0.0	0.0	0.0	15.762967334439198	0.0
28	0.0	0.0	0.0	16.06123197652163	0.0
29	0.0	0.0	0.0	16.393190953170855	0.0
30	0.0	0.0	0.0	16.76422738292714	0.0
31	0.0	0.0	0.0	17.09140136531836	0.0
32	0.0	0.0	0.0	17.399036621156053	0.0
33	0.0	0.0	0.0	17.72002998596402	0.0
34	0.0	0.0	0.0	18.053185211177748	0.0
35	0.0	0.0	0.0	18.400695419165498	0.0
36	0.0	0.0	0.0	18.71610629067245	0.0
37	0.0	0.0	0.0	19.05564150823019	0.0
38	0.0	0.0	0.0	19.45199853260176	0.0
39	0.0	0.0	0.0	20.00765599081281	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	20	7.029481E-4	45.0	31
CGACGGT	20	7.029481E-4	45.0	28
TTTGTCG	20	7.029481E-4	45.0	45
CTACGGA	20	7.029481E-4	45.0	34
CGCACGG	40	6.8012014E-9	45.0	2
ACGAGGT	20	7.029481E-4	45.0	31
TAATGCG	20	7.029481E-4	45.0	1
ACGACCA	90	0.0	42.5	28
TGGGCGA	130	0.0	41.53846	6
CGTTTTT	1195	0.0	41.23431	1
TACGGCT	520	0.0	40.673077	7
CGAGACA	95	0.0	40.263157	22
CACGGGA	135	0.0	40.0	4
TCCGATC	45	1.9244908E-8	40.0	14
ATTTGCG	40	3.453024E-7	39.375	1
ACGGCTG	600	0.0	38.625	8
TAGCGTG	35	6.241251E-6	38.571426	1
TTGTACG	35	6.241251E-6	38.571426	37
CTGATAG	35	6.241251E-6	38.571426	1
ACACGAC	100	0.0	38.25	26
>>END_MODULE