##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936668.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	278908
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11140949703845	31.0	31.0	33.0	28.0	34.0
2	31.158113069542644	31.0	31.0	34.0	28.0	34.0
3	30.884614281411793	31.0	30.0	34.0	27.0	34.0
4	34.86022631118505	35.0	35.0	37.0	32.0	37.0
5	35.12493366988397	35.0	35.0	37.0	33.0	37.0
6	35.12767292440518	36.0	35.0	37.0	32.0	37.0
7	31.222940898073915	35.0	35.0	37.0	0.0	37.0
8	33.145926255252625	35.0	35.0	37.0	17.0	37.0
9	36.298209445408524	39.0	35.0	39.0	32.0	39.0
10	36.09244625467896	37.0	35.0	39.0	32.0	39.0
11	36.36517059388759	37.0	35.0	39.0	32.0	39.0
12	36.423917564214726	38.0	35.0	39.0	32.0	39.0
13	36.343625855120685	38.0	35.0	39.0	32.0	39.0
14	37.41099215511925	39.0	36.0	40.0	32.0	41.0
15	37.5761254607254	39.0	36.0	41.0	32.0	41.0
16	37.54436588409081	39.0	36.0	40.0	33.0	41.0
17	37.42244754542717	39.0	36.0	40.0	32.0	41.0
18	37.29677169532606	39.0	36.0	40.0	32.0	41.0
19	37.06671375507336	39.0	36.0	40.0	32.0	41.0
20	37.01654667488921	39.0	35.0	40.0	32.0	41.0
21	37.05687538543175	39.0	35.0	40.0	32.0	41.0
22	37.13729258393449	39.0	35.0	40.0	32.0	41.0
23	37.19066143674617	39.0	35.0	40.0	32.0	41.0
24	37.10879214651426	39.0	35.0	40.0	32.0	41.0
25	36.77840363130495	38.0	35.0	40.0	31.0	41.0
26	36.75900296872087	38.0	35.0	40.0	31.0	41.0
27	36.81144319990821	39.0	35.0	40.0	31.0	41.0
28	36.77833909389476	39.0	35.0	40.0	31.0	41.0
29	36.721686003986974	39.0	35.0	40.0	31.0	41.0
30	36.50226239476817	38.0	35.0	40.0	31.0	41.0
31	36.47533953848581	38.0	35.0	40.0	31.0	41.0
32	36.28321525377544	38.0	35.0	40.0	30.0	41.0
33	36.06376654667489	38.0	35.0	40.0	30.0	41.0
34	35.89703056204913	38.0	35.0	40.0	29.0	41.0
35	35.82470563770132	38.0	35.0	40.0	29.0	41.0
36	35.73393735568718	38.0	35.0	40.0	28.0	41.0
37	35.63106830926327	38.0	35.0	40.0	27.0	41.0
38	35.48853026804538	38.0	35.0	40.0	27.0	41.0
39	35.425713855464885	38.0	35.0	40.0	26.0	41.0
40	35.30105984769171	38.0	34.0	40.0	26.0	41.0
41	35.15626299711733	38.0	34.0	40.0	25.0	41.0
42	35.17526926441694	38.0	34.0	40.0	25.0	41.0
43	35.13233395958524	38.0	34.0	40.0	25.0	41.0
44	34.911486941930676	38.0	34.0	40.0	24.0	41.0
45	34.880738451388986	38.0	34.0	40.0	24.0	41.0
46	34.75413039425187	38.0	34.0	40.0	23.0	41.0
47	34.68119236450729	38.0	34.0	40.0	23.0	41.0
48	34.62522050281813	37.0	34.0	40.0	23.0	41.0
49	34.486561877034724	37.0	34.0	40.0	23.0	41.0
50	34.31151849355343	37.0	33.0	40.0	23.0	41.0
51	32.56483499935462	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	22.0
10	25.0
11	16.0
12	8.0
13	7.0
14	10.0
15	13.0
16	38.0
17	65.0
18	149.0
19	301.0
20	502.0
21	809.0
22	1095.0
23	1586.0
24	2297.0
25	3313.0
26	4119.0
27	4845.0
28	4914.0
29	5507.0
30	6649.0
31	8265.0
32	10563.0
33	14548.0
34	22137.0
35	24756.0
36	27307.0
37	40305.0
38	53508.0
39	41215.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0264029715892	19.95998680568503	22.076096777431985	15.937513445293789
2	33.41101725300099	25.281454816642047	25.505543046452594	15.80198488390437
3	31.08551923931906	24.954823812870195	28.75894560213404	15.200711345676709
4	27.530583561604544	24.275388300084614	30.180202790884447	18.01382534742639
5	25.856913390795533	28.19280909834067	27.488992786151705	18.46128472471209
6	24.893871814361727	34.23530339753611	26.912100047327435	13.958724740774736
7	66.00886313766546	16.82203450600198	13.302235862721759	3.8668664936107966
8	75.98598821116641	7.108078649590547	11.99176789478968	4.914165244453368
9	69.99117988727465	7.495661651870868	14.314397579130036	8.19876088172444
10	39.90276363532061	25.116525879501484	21.376224418087688	13.604486067090225
11	29.71338219054312	25.2753596167912	27.181723005435483	17.829535187230196
12	26.065225809227417	21.5354166965451	32.317825232693224	20.081532261534267
13	22.895363345619344	24.891720567355545	34.272950220144274	17.939965866880836
14	20.14786237755819	28.164484346092618	31.83020924462547	19.857444031723723
15	18.455189524861247	25.557172974600945	37.58300228032183	18.404635220215983
16	20.836261419536193	25.626012878798743	32.58099444978272	20.956731251882342
17	21.10158188363188	24.626758644427554	31.081575286474393	23.190084185466176
18	21.934831557359416	24.545011258192666	32.74664046925868	20.77351671518924
19	22.14565376396518	26.26744302780845	30.552368522953806	21.03453468527256
20	23.745464454228635	25.189668277711647	31.20025241298206	19.86461485507766
21	23.39481119222109	27.11575143057926	30.640928191374933	18.848509185824717
22	21.401322299826468	23.533566624119782	31.459119136059204	23.60599193999455
23	19.742352316893026	26.57076885568001	31.354066573924015	22.332812253502947
24	21.834798571571987	23.178610868099874	31.85315587935807	23.13343468097007
25	19.814419091600097	28.03827785506332	29.99519554835286	22.15210750498372
26	19.001964805598977	26.447072152824585	31.433662713152728	23.11730032842371
27	19.719405682160428	26.321582744130684	31.591062285771653	22.367949287937243
28	17.998408077215426	25.800980968634818	34.2008834454372	21.99972750871255
29	19.569535474063134	25.366070532218508	31.80475282171899	23.25964117199937
30	20.490627733876405	26.509099774836148	33.110918295638704	19.889354195648746
31	21.981083367992312	24.967372753739582	31.277338764036887	21.774205114231215
32	22.650838269250077	26.168485665524116	29.24584450786639	21.934831557359416
33	23.120168657765287	24.175355314297185	29.13613091055115	23.56834511738638
34	20.61862693074419	25.152738537438868	31.920203077717385	22.30843145409956
35	19.17370602492578	24.873434967803004	30.1744661322013	25.778392875069915
36	21.570912272147087	26.35636123739728	30.5989788747544	21.473747615701235
37	20.077229767521906	25.605576032240023	31.996572346436817	22.320621853801253
38	20.777102126866207	26.084945573450746	29.075895994378076	24.062056305304974
39	21.475540321539718	24.687710642936022	29.882255080528346	23.95449395499591
40	22.407747357551592	24.358569850990293	30.22717168385274	23.006511107605377
41	19.09267572102629	24.606321797868834	29.499691654595782	26.801310826509102
42	20.226382893283805	24.336340298593086	30.232908342535893	25.20436846558722
43	21.815437348516355	24.269651641401467	28.567484618583904	25.347426391498274
44	20.31781089104651	24.644685702812396	30.193468814089236	24.84403459205186
45	20.297732585655485	24.374704203536652	29.256242201729606	26.071321009078265
46	23.474765872617496	25.236637170679938	29.132186957706484	22.156409998996086
47	19.857444031723723	23.831155793308188	33.14354554189912	23.16785463306897
48	20.174035882800062	23.952342707989732	30.579617651698772	25.294003757511437
49	19.813343468097006	22.4066717340485	33.33285527844307	24.44712951941142
50	20.19626543519727	22.942332238587635	32.643380612961984	24.218021713253115
51	19.790396833364408	23.227013925738955	29.574985299812123	27.407603941084513
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	98.0
1	246.0
2	394.0
3	2563.5
4	4733.0
5	3213.5
6	1694.0
7	1583.5
8	1473.0
9	1468.0
10	1463.0
11	1394.0
12	1325.0
13	1266.5
14	1208.0
15	1158.0
16	1108.0
17	1099.0
18	1090.0
19	978.5
20	867.0
21	914.0
22	961.0
23	1089.5
24	1218.0
25	1425.5
26	2045.0
27	2457.0
28	2851.5
29	3246.0
30	3933.0
31	4620.0
32	5321.5
33	6023.0
34	6537.5
35	7052.0
36	7956.0
37	8860.0
38	9654.5
39	10449.0
40	11398.0
41	12347.0
42	14553.0
43	16759.0
44	18399.5
45	20040.0
46	25534.5
47	31029.0
48	28621.5
49	26214.0
50	25372.0
51	24530.0
52	21174.0
53	17818.0
54	16339.5
55	14861.0
56	13661.0
57	12461.0
58	11800.0
59	11139.0
60	9943.0
61	8747.0
62	7898.0
63	7049.0
64	6040.5
65	5032.0
66	4097.0
67	3162.0
68	2755.5
69	2349.0
70	1928.0
71	1507.0
72	1189.0
73	871.0
74	677.5
75	349.0
76	214.0
77	191.0
78	168.0
79	123.5
80	79.0
81	52.5
82	26.0
83	23.5
84	21.0
85	17.5
86	14.0
87	10.0
88	6.0
89	5.5
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	278908.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.203939892593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.01390676264181	27.157989715432596
2	9.034988770102498	6.542043807260871
3	3.7321174900588123	4.053520718465528
4	2.0249996021402947	2.932518555136479
5	1.3279983938210416	2.403938701367852
6	0.9829987792487023	2.135305723104739
7	0.7349990864695567	1.8626903923358216
8	0.6149992355970237	1.7812316287636232
9	0.4449994468949366	1.4499659904859272
>10	6.0019925399175635	43.22998477551737
>50	0.061999922938168764	1.5451822340558605
>100	0.017999977627210288	1.374299850158399
>500	9.99998757067238E-4	0.20382792825057394
>1k	0.004999993785336191	3.3274999796643425
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2587	0.9275460008318155	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGC	1956	0.7013065240150874	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCG	1840	0.6597157485622499	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC	1677	0.6012735382276593	No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1131	0.4055100606651656	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTT	563	0.2018586774133406	No Hit
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	435	0.1559654079481406	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	321	0.11509171483069687	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCT	318	0.11401609132760623	No Hit
GAACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCT	308	0.11043067965063749	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTC	280	0.10039152695512497	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13803834956329686	0.0
2	0.0	0.0	0.0	0.9841955053279218	0.0
3	0.0	0.0	0.0	1.2240595465171311	0.0
4	0.0	0.0	0.0	1.6578943594303497	0.0
5	0.0	0.0	0.0	3.182052863309765	0.0
6	0.0	0.0	0.0	3.9324795272993245	0.0
7	0.0	0.0	0.0	4.552038665079524	0.0
8	0.0	0.0	0.0	5.558463722804652	0.0
9	0.0	0.0	0.0	5.872545785707115	0.0
10	0.0	0.0	0.0	7.382362642878656	0.0
11	0.0	0.0	0.0	8.335006525449252	0.0
12	0.0	0.0	0.0	9.99541067305348	0.0
13	0.0	0.0	0.0	10.504539131183042	0.0
14	0.0	0.0	0.0	10.76770834827255	0.0
15	0.0	0.0	0.0	11.145969280192752	0.0
16	0.0	0.0	0.0	11.758716135786711	0.0
17	0.0	0.0	0.0	12.468269106658827	0.0
18	0.0	0.0	0.0	13.262437793107404	0.0
19	0.0	0.0	0.0	13.760092933870666	0.0
20	0.0	0.0	0.0	14.198230240796248	0.0
21	0.0	0.0	0.0	14.745005521533983	0.0
22	0.0	0.0	0.0	15.263456050023663	0.0
23	0.0	0.0	0.0	15.792304272376555	0.0
24	0.0	0.0	0.0	16.224346379451287	0.0
25	0.0	0.0	0.0	16.612646464067005	0.0
26	0.0	0.0	0.0	16.984095113800965	0.0
27	0.0	0.0	0.0	17.369526869075106	0.0
28	0.0	0.0	0.0	17.74563655398913	0.0
29	0.0	0.0	0.0	18.128558521089392	0.0
30	0.0	0.0	0.0	18.562034792834915	0.0
31	0.0	0.0	0.0	18.9650350653262	0.0
32	0.0	0.0	0.0	19.35405223227731	0.0
33	0.0	0.0	0.0	19.72801067018515	0.0
34	0.0	0.0	0.0	20.102686190428386	0.0
35	0.0	0.0	0.0	20.526123309478393	0.0
36	0.0	0.0	0.0	20.913706311758716	0.0
37	0.0	0.0	0.0	21.29877952586516	0.0
38	0.0	0.0	0.0	21.70034563368566	0.0
39	0.0	0.0	0.0	22.12700962324494	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	25	3.883185E-5	45.0	1
GATACCA	20	7.0240913E-4	45.0	45
TCGCACT	20	7.0240913E-4	45.0	25
CGACGTT	20	7.0240913E-4	45.0	27
CCGGCCG	20	7.0240913E-4	45.0	32
TAGCGTG	20	7.0240913E-4	45.0	1
TCGGCTG	20	7.0240913E-4	45.0	18
CTCGGCT	20	7.0240913E-4	45.0	17
GCCCTTA	20	7.0240913E-4	45.0	21
GCGATCA	20	7.0240913E-4	45.0	9
CGATGCA	20	7.0240913E-4	45.0	10
TCGACGT	20	7.0240913E-4	45.0	26
CGTTTTT	1255	0.0	43.924305	1
ACGGGCT	45	1.9193976E-8	40.000004	5
AGCGACT	40	3.4456752E-7	39.375	19
CGACTGG	40	3.4456752E-7	39.375	2
CGTTTTA	115	0.0	39.130432	1
AACGGGA	105	0.0	38.57143	4
GCCCCAC	35	6.2312884E-6	38.571426	34
GTGGACG	35	6.2312884E-6	38.571426	26
>>END_MODULE