FastQCFastQC Report
Sat 14 Jan 2017
SRR2936665.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936665.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30757
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG7002.275904672107163No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGC5881.9117599245700163No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4851.5768768085313911No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTC3271.0631726111129174No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTT1490.4844425659199532No Hit
GCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC990.3218779464837273No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCT750.24384692915433884No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTC650.21133400526709367No Hit
GAACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCT610.1983288357121956No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTACGCGTT610.1983288357121956No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTA410.13330298793770523No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATG390.1268004031602562No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG360.11704652599408266No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGC340.11054394121663362No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT340.11054394121663362No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGT320.10404135643918458No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA320.10404135643918458No Hit
AAGATGGGGCGATTTGGACGGGAATATTTCCAACCAACAATAACTCAAAAA320.10404135643918458No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT310.10079006405046005No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGG206.926603E-445.0000043
ATGGGCT206.926603E-445.0000045
AGGGGAC206.926603E-445.0000046
CGAGGAA206.926603E-445.00000419
CCACCGA406.4501364E-945.00000415
CAGGGAG206.926603E-445.0000045
AGACGGG206.926603E-445.0000043
CCGAGGA206.926603E-445.00000418
CGATGAA206.926603E-445.00000419
CCGATGA206.926603E-445.00000418
AAGGGGT302.0971474E-644.9999965
AGCGGGG302.0971474E-644.9999964
GTGCGGG302.0971474E-644.9999963
TACGGCT800.042.1875047
ACGGCTG800.042.1875048
CGTTTTT1950.040.3846171
GGGAATG850.039.7058837
TGCGGGG403.3142533E-739.3750044
AGGGATG356.0523325E-638.5714266
TGTAGGG356.0523325E-638.5714263
Produced by FastQC (version 0.11.5)