##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936664.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	208391
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255778800428043	31.0	31.0	33.0	30.0	34.0
2	31.32272027102898	31.0	31.0	34.0	30.0	34.0
3	31.16415296245999	31.0	31.0	34.0	28.0	34.0
4	35.11140116415776	35.0	35.0	37.0	32.0	37.0
5	35.28151407690351	35.0	35.0	37.0	33.0	37.0
6	35.24140677860368	37.0	35.0	37.0	33.0	37.0
7	30.91459803926273	35.0	35.0	37.0	0.0	37.0
8	33.01918029089548	36.0	35.0	37.0	17.0	37.0
9	36.4545301860445	39.0	35.0	39.0	32.0	39.0
10	36.16585649092331	37.0	35.0	39.0	32.0	39.0
11	36.42896766175123	38.0	35.0	39.0	32.0	39.0
12	36.45148302949743	38.0	35.0	39.0	32.0	39.0
13	36.392027486791655	38.0	35.0	39.0	32.0	39.0
14	37.35929574693725	39.0	36.0	41.0	32.0	41.0
15	37.58647926254013	39.0	36.0	41.0	32.0	41.0
16	37.53506629364992	39.0	36.0	41.0	33.0	41.0
17	37.4958803403218	39.0	36.0	41.0	33.0	41.0
18	37.40782951279086	39.0	36.0	40.0	32.0	41.0
19	37.24593192604287	39.0	36.0	40.0	32.0	41.0
20	37.22305665791709	39.0	35.0	40.0	32.0	41.0
21	37.22376206266105	39.0	35.0	40.0	32.0	41.0
22	37.31651558848511	39.0	35.0	40.0	32.0	41.0
23	37.38314514542375	39.0	35.0	41.0	32.0	41.0
24	37.34567711657413	39.0	35.0	41.0	32.0	41.0
25	36.9928883684996	39.0	35.0	40.0	31.0	41.0
26	36.98449069297619	39.0	35.0	40.0	31.0	41.0
27	37.00373816527585	39.0	35.0	40.0	32.0	41.0
28	37.00774505616845	39.0	35.0	40.0	32.0	41.0
29	36.97611701081141	39.0	35.0	40.0	31.0	41.0
30	36.76660700318152	39.0	35.0	40.0	31.0	41.0
31	36.74289676617512	39.0	35.0	40.0	31.0	41.0
32	36.64661621663124	39.0	35.0	40.0	31.0	41.0
33	36.55326285684123	39.0	35.0	40.0	30.0	41.0
34	36.474622224568236	39.0	35.0	40.0	30.0	41.0
35	36.435940131771524	39.0	35.0	40.0	30.0	41.0
36	36.36827406173971	39.0	35.0	40.0	30.0	41.0
37	36.26079341238345	39.0	35.0	40.0	30.0	41.0
38	36.09933730343441	38.0	35.0	40.0	30.0	41.0
39	36.05312129602526	38.0	35.0	40.0	29.0	41.0
40	35.939056869058646	38.0	35.0	40.0	29.0	41.0
41	35.76056547547639	38.0	35.0	40.0	28.0	41.0
42	35.747109999952016	38.0	35.0	40.0	29.0	41.0
43	35.72246402195872	38.0	35.0	40.0	29.0	41.0
44	35.52627032837311	38.0	34.0	40.0	28.0	41.0
45	35.47239084221488	38.0	34.0	40.0	28.0	41.0
46	35.44519676953419	38.0	34.0	40.0	28.0	41.0
47	35.40496950444117	38.0	34.0	40.0	28.0	41.0
48	35.28242102586004	38.0	34.0	40.0	27.0	41.0
49	35.17687903988176	37.0	34.0	40.0	27.0	41.0
50	35.012145438142724	37.0	34.0	40.0	27.0	41.0
51	33.41160606744053	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	19.0
9	22.0
10	23.0
11	24.0
12	20.0
13	13.0
14	21.0
15	34.0
16	50.0
17	71.0
18	117.0
19	211.0
20	297.0
21	528.0
22	779.0
23	972.0
24	1302.0
25	1756.0
26	2183.0
27	2478.0
28	3019.0
29	3604.0
30	4239.0
31	5690.0
32	7710.0
33	10739.0
34	16301.0
35	17725.0
36	21314.0
37	31331.0
38	41742.0
39	34056.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.07915888881958	20.488408808441825	23.140154805149933	12.292277497588668
2	32.663598715875445	23.782696949484382	26.261690763996526	17.292013570643647
3	33.534557634446784	22.758660402800505	27.51750315512666	16.18927880762605
4	29.690821580586498	25.641702376782106	25.53949066898283	19.127985373648574
5	26.12636822127635	30.22971241560336	24.57831672193137	19.06560264118892
6	27.03811584953285	35.15650867839782	24.18098670288064	13.62438876918869
7	71.33177536457909	18.203761198900146	7.021416471920572	3.4430469646001987
8	83.8500703005408	5.779520228800668	6.828030001295642	3.54237946936288
9	78.21786929377949	6.8064359785211455	9.14098977403055	5.834704953668825
10	44.18089072944609	24.448752585284396	16.495913931023892	14.874442754245626
11	32.13238575562284	26.04767000494263	22.81000619028653	19.009938049147994
12	28.917755565259533	23.606585697079048	27.49063059345173	19.985028144209682
13	26.223781257347966	24.232332490366666	29.63563685571834	19.90824939656703
14	21.945765412133923	26.61535287032549	29.835261599589234	21.60362011795135
15	21.500448675806535	25.43296015662865	32.50428281451694	20.562308353047875
16	25.775585317983985	24.524571598581513	28.91871529960507	20.781127783829437
17	26.6038360581791	24.60902822098843	26.170516001170874	22.6166197196616
18	26.57984269954077	24.89071025140241	27.88460154229309	20.64484550676373
19	26.308717746927652	26.091337917664394	26.090378183318858	21.509566152089103
20	26.36870114352347	27.257415147487173	26.645104635037022	19.728779073952328
21	27.237740593403746	25.696887101650262	28.07990748160909	18.9854648233369
22	25.454074312230375	23.614263571843313	28.431650119246992	22.50001199667932
23	23.628179719853545	25.56636323065775	28.306404787154914	22.499052262333784
24	23.519729738808298	26.445479891166123	28.23394484406716	21.800845525958415
25	24.958371522762498	26.492027006924484	26.554409739384138	21.99519173092888
26	22.741865051753674	26.860564995609217	26.375419283942204	24.022150668694906
27	23.104164767192444	26.336070175775344	27.90523583072206	22.654529226310157
28	21.865147727109136	26.100935261119723	28.816503591805787	23.217413419965354
29	22.650690288928025	26.5692856217399	28.471479094586616	22.308544994745453
30	24.21217806911047	25.47710793652317	29.020927007404353	21.28978698696201
31	25.52509465379983	26.30056000499062	26.7674707640925	21.406874577117055
32	25.589876722123318	26.77706810754783	26.493466608442784	21.139588561886068
33	25.851884198453867	26.000643022011506	26.375419283942204	21.77205349559242
34	23.183822717871692	25.996324217456607	28.066951067944395	22.752901996727307
35	23.20925567802832	27.011243287857923	27.63699008114554	22.14251095296822
36	26.56448695001224	26.21658324975647	26.342308449021314	20.876621351209984
37	23.981361959009746	27.92443051763272	26.47667125739595	21.617536265961583
38	24.295674957171855	27.813101333550872	25.41280573537245	22.478417973904826
39	24.600870479051398	25.55820548872072	26.389815299125203	23.45110873310268
40	25.416164805581815	24.838404729570854	27.8428530982624	21.90257736658493
41	23.070094197926014	25.20886218694665	28.06551146642609	23.655532148701237
42	23.541323761582795	25.880196361647094	27.694574141877524	22.883905734892583
43	23.754384786291155	24.923821086323304	28.23586431275823	23.085929814627313
44	23.20397713912789	25.36961768982346	28.787231694267028	22.639173476781625
45	23.052818979706416	25.438238695529076	27.4911104606245	24.017831864140007
46	23.087369416145613	25.664256133902136	27.426808259473777	23.821566190478475
47	22.504810668406986	26.391254900643503	28.527143686627543	22.576790744321972
48	22.249521332495164	26.97717271859149	27.8687659255918	22.904540023321545
49	23.307148581272706	25.703605242068996	28.357270707468174	22.631975469190127
50	21.8814632109832	26.373019948078376	28.757959796728265	22.987557044210163
51	22.4213137803456	25.904189720285427	27.972897102082143	23.70159939728683
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	191.0
2	318.0
3	626.5
4	935.0
5	697.5
6	460.0
7	430.5
8	401.0
9	414.0
10	427.0
11	429.5
12	432.0
13	428.0
14	424.0
15	406.0
16	388.0
17	440.0
18	492.0
19	469.0
20	446.0
21	519.0
22	592.0
23	729.5
24	867.0
25	1098.5
26	1441.5
27	1553.0
28	1969.0
29	2385.0
30	2733.0
31	3081.0
32	3933.0
33	4785.0
34	5414.5
35	6044.0
36	6616.5
37	7189.0
38	7921.0
39	8653.0
40	9234.5
41	9816.0
42	10821.0
43	11826.0
44	12157.5
45	12489.0
46	14417.0
47	16345.0
48	16474.5
49	16604.0
50	16142.0
51	15680.0
52	14772.0
53	13864.0
54	12748.0
55	11632.0
56	11574.0
57	11516.0
58	11231.5
59	10947.0
60	9898.5
61	8850.0
62	8284.0
63	7718.0
64	6835.0
65	5952.0
66	5289.0
67	4626.0
68	3946.0
69	3266.0
70	2786.0
71	2306.0
72	1935.0
73	1564.0
74	1281.5
75	774.5
76	550.0
77	428.0
78	306.0
79	205.0
80	104.0
81	89.5
82	75.0
83	55.5
84	36.0
85	25.5
86	15.0
87	16.0
88	17.0
89	15.5
90	14.0
91	8.5
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	208391.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.137006876496585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.72777655165649	26.89799463508501
2	9.370018882267834	7.334289868564381
3	4.6237033767380264	5.428737325508299
4	2.9831530935040096	4.6700673253643386
5	2.2499325633291645	4.4027813101333555
6	1.8440864170283726	4.3303213670456016
7	1.6405502832340175	4.494435940131772
8	1.4701194242134432	4.602885921177018
9	1.2347041369332254	4.349036186783498
>10	5.8228499963216365	31.67747167584013
>50	0.019617940606684814	0.5571257875819974
>100	0.013487334167095808	1.2548526567846021
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	434	0.20826235298069495	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTC	373	0.17899045544193368	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGC	372	0.1785105882691671	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCG	328	0.15739643266743766	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	238	0.11420838711844561	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	221	0.10605064518141379	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05038605314049071	0.0
2	0.0	0.0	0.0	0.2845612334505809	0.0
3	0.0	0.0	0.0	0.36901785585749863	0.0
4	0.0	0.0	0.0	0.5628841936551963	0.0
5	0.0	0.0	0.0	1.0513889755315728	0.0
6	0.0	0.0	0.0	1.3796181217039123	0.0
7	0.0	0.0	0.0	1.6569813475629946	0.0
8	0.0	0.0	0.0	2.0792644595975833	0.0
9	0.0	0.0	0.0	2.2673723913220822	0.0
10	0.0	0.0	0.0	2.802424288956817	0.0
11	0.0	0.0	0.0	3.219428862090973	0.0
12	0.0	0.0	0.0	3.804386945693432	0.0
13	0.0	0.0	0.0	4.03328358710309	0.0
14	0.0	0.0	0.0	4.140293966630037	0.0
15	0.0	0.0	0.0	4.298650133643007	0.0
16	0.0	0.0	0.0	4.542902524581196	0.0
17	0.0	0.0	0.0	4.8183462817492115	0.0
18	0.0	0.0	0.0	5.156172771376883	0.0
19	0.0	0.0	0.0	5.369233796085243	0.0
20	0.0	0.0	0.0	5.562140399537408	0.0
21	0.0	0.0	0.0	5.813110930894329	0.0
22	0.0	0.0	0.0	6.045846509686119	0.0
23	0.0	0.0	0.0	6.266105541985978	0.0
24	0.0	0.0	0.0	6.475807496484973	0.0
25	0.0	0.0	0.0	6.6480798115081745	0.0
26	0.0	0.0	0.0	6.844825352342472	0.0
27	0.0	0.0	0.0	7.060765580087431	0.0
28	0.0	0.0	0.0	7.25175271484853	0.0
29	0.0	0.0	0.0	7.473931215839456	0.0
30	0.0	0.0	0.0	7.751294441698538	0.0
31	0.0	0.0	0.0	7.978751481589896	0.0
32	0.0	0.0	0.0	8.19709104519869	0.0
33	0.0	0.0	0.0	8.419269546189614	0.0
34	0.0	0.0	0.0	8.660162866918437	0.0
35	0.0	0.0	0.0	8.900096453301726	0.0
36	0.0	0.0	0.0	9.117476282564986	0.0
37	0.0	0.0	0.0	9.350211861356776	0.0
38	0.0	0.0	0.0	9.653008047372488	0.0
39	0.0	0.0	0.0	10.173184062651458	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACGGG	30	2.1569213E-6	45.000004	3
CACAACG	30	2.1569213E-6	45.000004	12
GTTGATC	40	6.7684596E-9	45.0	16
TACGGGC	35	1.2055352E-7	45.0	4
TAGCCGG	20	7.019982E-4	45.0	2
GTTCGTT	20	7.019982E-4	45.0	23
CTGTTAG	40	6.7684596E-9	45.0	2
GCGATTT	20	7.019982E-4	45.0	9
ACGCTGG	20	7.019982E-4	45.0	2
CGGTCTA	20	7.019982E-4	45.0	31
CGTCATA	20	7.019982E-4	45.0	38
TACACGG	20	7.019982E-4	45.0	2
ACACGGG	55	6.002665E-11	40.909092	3
TGATCCG	40	3.4400728E-7	39.375	14
GCGAATG	35	6.2236977E-6	38.571426	1
GTCTCAC	35	6.2236977E-6	38.571426	22
TCGATCA	35	6.2236977E-6	38.571426	17
CTACGAA	35	6.2236977E-6	38.571426	11
CATACGA	35	6.2236977E-6	38.571426	18
ATTGCGG	35	6.2236977E-6	38.571426	2
>>END_MODULE