##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936663.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	619932
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.055680300420047	31.0	31.0	33.0	28.0	34.0
2	31.09137937709297	31.0	31.0	34.0	28.0	34.0
3	30.859658801287882	31.0	30.0	34.0	27.0	34.0
4	34.832999425743466	35.0	35.0	37.0	32.0	37.0
5	35.03852035384526	35.0	35.0	37.0	32.0	37.0
6	34.97826213197577	35.0	35.0	37.0	32.0	37.0
7	31.031621210068202	35.0	35.0	37.0	0.0	37.0
8	32.84015021002303	35.0	33.0	37.0	17.0	37.0
9	35.87137621545589	38.0	34.0	39.0	32.0	39.0
10	35.941225811863234	37.0	35.0	39.0	32.0	39.0
11	36.161198324977576	37.0	35.0	39.0	32.0	39.0
12	36.220485472600224	37.0	35.0	39.0	32.0	39.0
13	35.99969835401302	38.0	35.0	39.0	31.0	39.0
14	37.13573424182007	39.0	36.0	40.0	32.0	41.0
15	37.1157110779892	39.0	36.0	40.0	31.0	41.0
16	37.29964415452017	39.0	36.0	40.0	32.0	41.0
17	36.977510436628535	39.0	35.0	40.0	31.0	41.0
18	36.9035587774143	39.0	35.0	40.0	31.0	41.0
19	36.69274210719885	38.0	35.0	40.0	31.0	41.0
20	36.512804630185244	38.0	35.0	40.0	30.0	41.0
21	36.757376615499766	38.0	35.0	40.0	31.0	41.0
22	36.82207887316673	39.0	35.0	40.0	31.0	41.0
23	36.9076285786183	39.0	35.0	40.0	31.0	41.0
24	36.7596075053393	38.0	35.0	40.0	31.0	41.0
25	36.61658698050754	38.0	35.0	40.0	31.0	41.0
26	36.42460786021693	38.0	35.0	40.0	30.0	41.0
27	36.48546614790009	38.0	35.0	40.0	31.0	41.0
28	36.38287263764413	38.0	35.0	40.0	30.0	41.0
29	36.29076576140609	38.0	35.0	40.0	30.0	41.0
30	36.08692566281463	38.0	35.0	40.0	30.0	41.0
31	36.037828342463364	38.0	35.0	40.0	30.0	41.0
32	35.843473155120236	38.0	35.0	40.0	29.0	41.0
33	35.621989185910714	38.0	35.0	40.0	27.0	41.0
34	35.463742797597156	38.0	35.0	40.0	27.0	41.0
35	35.412325867998426	38.0	35.0	40.0	26.0	41.0
36	35.24851274010698	38.0	34.0	40.0	25.0	41.0
37	35.20175115980462	38.0	34.0	40.0	26.0	41.0
38	34.97935902647387	38.0	34.0	40.0	24.0	41.0
39	35.0103511352858	38.0	34.0	40.0	25.0	41.0
40	34.889234625733145	38.0	34.0	40.0	24.0	41.0
41	34.717294800074846	38.0	34.0	40.0	23.0	41.0
42	34.66297110005613	38.0	34.0	40.0	23.0	41.0
43	34.64489653703954	38.0	34.0	40.0	23.0	41.0
44	34.51720995205926	37.0	33.0	40.0	23.0	41.0
45	34.446119897020964	37.0	33.0	40.0	23.0	41.0
46	34.33703696534459	37.0	33.0	40.0	23.0	41.0
47	34.15093590909971	37.0	33.0	40.0	23.0	41.0
48	34.1415429434196	37.0	33.0	40.0	23.0	41.0
49	34.058666111767096	36.0	33.0	40.0	23.0	41.0
50	33.890813831194386	36.0	33.0	40.0	22.0	41.0
51	32.17519824754973	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	22.0
9	44.0
10	55.0
11	39.0
12	42.0
13	19.0
14	32.0
15	47.0
16	121.0
17	228.0
18	625.0
19	1095.0
20	1807.0
21	2623.0
22	3382.0
23	4473.0
24	5912.0
25	7821.0
26	10002.0
27	10730.0
28	11917.0
29	13540.0
30	16263.0
31	20350.0
32	26737.0
33	36037.0
34	52894.0
35	56502.0
36	62082.0
37	85885.0
38	107026.0
39	81569.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.68679145454663	16.694734261177032	19.795396914500298	14.823077369776039
2	30.67594510365653	31.914790654458873	21.81303755895808	15.596226682926515
3	28.605556738481	31.8136505294129	25.78089209784299	13.799900634263112
4	26.207551796003433	21.581560558254775	35.24499461231232	16.96589303342947
5	30.98743087951582	25.00048392404328	24.492686294625862	19.519398901815038
6	23.086403024847886	38.05885161598369	26.227392681778	12.627352677390425
7	61.177838859745904	16.671667215113917	18.387661872592478	3.762832052547699
8	69.1669408902912	12.838504868275876	13.158862584928668	4.835691656504261
9	64.56853332300963	6.534265048424666	13.756024854338863	15.14117677422685
10	42.46062471367827	20.994883309782363	20.636456901724706	15.908035074814658
11	32.25757018511708	21.9833788221934	26.888594232915867	18.870456759773653
12	27.342031061471257	19.247272281476032	31.17616125639586	22.234535400656846
13	23.862294574243627	20.766955085396464	36.99050863643109	18.380241703928817
14	20.564997451333372	26.641631662827535	30.965331681539265	21.828039204299827
15	18.165379428711535	22.979133195253674	40.037778336978896	18.817709039055895
16	21.364762586864366	23.656788163863133	29.626475161791937	25.35197408748056
17	21.740287644451328	23.667111876786485	32.715523638076434	21.877076840685753
18	22.421330081363763	23.54758263809579	31.542007833117182	22.489079447423265
19	22.01434996096346	26.022208887426363	28.403760412432334	23.559680739177846
20	24.650768148764705	24.814818399437357	32.82779401611789	17.706619435680043
21	23.27335901356923	29.818270390946104	29.036087828987693	17.872282766496973
22	22.19501493712214	21.220391913951854	32.07997006123252	24.50462308769349
23	23.011556106153577	27.211210261770646	30.656265525896387	19.120968106179387
24	24.214591277753044	22.308737087293444	28.79735196763516	24.67931966731835
25	21.010046263138538	30.34913506642664	27.12958840647039	21.511230263964435
26	20.96471871108444	23.42950517153494	30.518508481575395	25.087267635805215
27	23.764219301471776	25.84960931198906	27.596091184194393	22.79008020234477
28	19.084673802933224	26.396282172883478	32.208209932702296	22.310834091481002
29	26.69163714730003	23.400308420923587	27.585283547227764	22.32277088454863
30	24.02440912874315	26.632598414019604	29.835046424446553	19.507946032790695
31	25.38068691404864	25.5571578818322	26.194324538820386	22.867830665298776
32	27.352838698437893	29.234012762690103	24.510107560184018	18.903040978687986
33	24.29960060135628	23.430473019621505	24.90805443177639	27.361871947245824
34	26.31530554964093	24.727712071646568	29.56759773652594	19.389384642186563
35	24.061832588090308	27.59592987617997	26.127704328861874	22.214533206867852
36	27.705941942019447	28.461992605640617	24.818044559725905	19.014020892614028
37	24.964350928811545	28.521676570978755	28.122600543285394	18.391371956924306
38	21.823683887910285	30.15330713691179	23.12898834065672	24.894020634521205
39	24.092319802817084	26.644212591058373	28.35020615164244	20.913261454482104
40	22.23179316441158	25.754598891491327	28.33972113070466	23.673886813392436
41	23.024138131278914	26.31095023325139	28.201318854325958	22.46359278114374
42	21.366053050979787	26.911661278978983	29.79617119296955	21.92611447707168
43	26.02672551183033	23.597910738597136	25.33519805398011	25.04016569559242
44	23.043656401024627	23.182058677403326	29.296116348244645	24.478168573327398
45	21.345244317118652	22.538278391823617	28.281650245510797	27.834827045546927
46	25.99268952078615	26.850041617467724	26.111412219404713	21.045856642341416
47	19.758134763167572	23.758734828981243	34.64928411503197	21.83384629281921
48	22.26534523141248	25.61732577121362	27.800145822445042	24.317183174928864
49	21.125704109482975	22.391972022737978	33.458669660543414	23.023654207235634
50	22.28421826910048	22.935096107314994	29.588406470387074	25.19227915319745
51	21.68576553557487	22.516179193847066	27.972261473839065	27.825793796738996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144.0
1	636.0
2	1128.0
3	5332.0
4	9536.0
5	6517.0
6	3498.0
7	3296.0
8	3094.0
9	3053.0
10	3012.0
11	2849.5
12	2687.0
13	2618.0
14	2549.0
15	2462.0
16	2375.0
17	2345.5
18	2316.0
19	2268.5
20	2221.0
21	2424.5
22	2628.0
23	2642.5
24	2657.0
25	3150.0
26	3745.5
27	3848.0
28	4994.0
29	6140.0
30	7340.5
31	8541.0
32	9505.5
33	10470.0
34	12583.5
35	14697.0
36	15870.0
37	17043.0
38	18452.0
39	19861.0
40	21476.5
41	23092.0
42	25965.0
43	28838.0
44	32003.5
45	35169.0
46	65463.0
47	95757.0
48	71543.0
49	47329.0
50	46076.5
51	44824.0
52	40259.5
53	35695.0
54	34191.5
55	32688.0
56	31496.5
57	30305.0
58	29811.0
59	29317.0
60	27176.5
61	25036.0
62	23257.0
63	21478.0
64	18721.5
65	15965.0
66	12877.5
67	9790.0
68	8766.5
69	7743.0
70	6624.0
71	5505.0
72	4738.5
73	3972.0
74	3192.0
75	1925.5
76	1439.0
77	1037.0
78	635.0
79	538.0
80	441.0
81	333.5
82	226.0
83	147.0
84	68.0
85	68.5
86	69.0
87	47.5
88	26.0
89	18.5
90	11.0
91	10.0
92	9.0
93	6.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	619932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.08700895108612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63195877813115	22.45452411794722
2	8.316251991218166	5.004222961985374
3	3.556573263376973	3.2101995483124957
4	2.109414606314163	2.538639045669041
5	1.4128539559962592	2.125427481031844
6	1.0389732676147279	1.8755758801598101
7	0.831993417297937	1.752253537544143
8	0.6635471090937748	1.5971318248617374
9	0.5908184301896471	1.5998363457824305
>10	6.645039022581696	42.54685811888844
>50	0.1656955383575261	3.0579083553491935
>100	0.027794090305130952	1.5140787360300023
>500	0.0026725086831856687	0.5348726368598818
>1k	0.004810515629734204	3.495970009830719
>5k	0.0	0.0
>10k+	0.0016035052099114013	6.692501399747698
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	14606	2.356064858726441	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	14468	2.3338043527354615	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	12542	2.0231251169483104	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4875	0.7863765703335204	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3382	0.545543704793429	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	2721	0.43891910725692496	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	2717	0.4382738751992154	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC	2307	0.372137589283986	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTA	1779	0.28696695766632474	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	1394	0.22486337211177998	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	1292	0.20840995464018633	No Hit
GAATCTATCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	1272	0.20518379435163855	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT	934	0.15066168547518116	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	754	0.12162624287825115	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08452539955995174	0.0
2	0.0	0.0	0.0	0.8449313795706626	0.0
3	1.6130801442738882E-4	0.0	0.0	0.98655981623791	0.0
4	1.6130801442738882E-4	0.0	0.0	1.6140479923604525	0.0
5	1.6130801442738882E-4	0.0	0.0	4.196266687314092	0.0
6	1.6130801442738882E-4	0.0	0.0	4.65793022460528	0.0
7	1.6130801442738882E-4	0.0	0.0	5.370105108302201	0.0
8	1.6130801442738882E-4	0.0	0.0	6.211810327584316	0.0
9	1.6130801442738882E-4	0.0	0.0	6.376667118329107	0.0
10	1.6130801442738882E-4	0.0	0.0	9.410548253679435	0.0
11	1.6130801442738882E-4	0.0	0.0	9.907376938115794	0.0
12	1.6130801442738882E-4	0.0	0.0	13.06498132053193	0.0
13	1.6130801442738882E-4	0.0	0.0	13.350173890039553	0.0
14	1.6130801442738882E-4	0.0	0.0	13.521483001361439	0.0
15	1.6130801442738882E-4	0.0	0.0	13.998793416052083	0.0
16	3.2261602885477763E-4	0.0	0.0	14.378028557970874	0.0
17	3.2261602885477763E-4	0.0	0.0	14.732099649638993	0.0
18	3.2261602885477763E-4	0.0	0.0	15.093913525999625	0.0
19	3.2261602885477763E-4	0.0	0.0	15.441209681061794	0.0
20	3.2261602885477763E-4	0.0	0.0	15.69656026790035	0.0
21	3.2261602885477763E-4	0.0	0.0	15.960782795532413	0.0
22	3.2261602885477763E-4	0.0	0.0	16.28839937283444	0.0
23	3.2261602885477763E-4	0.0	0.0	16.56504261757741	0.0
24	3.2261602885477763E-4	0.0	0.0	16.794745230122015	0.0
25	3.2261602885477763E-4	0.0	0.0	17.014608053786546	0.0
26	3.2261602885477763E-4	0.0	0.0	17.23301910532123	0.0
27	3.2261602885477763E-4	0.0	0.0	17.490305388332914	0.0
28	3.2261602885477763E-4	0.0	0.0	17.719362768819806	0.0
29	3.2261602885477763E-4	0.0	0.0	17.950355845479827	0.0
30	3.2261602885477763E-4	0.0	0.0	18.223772929934253	0.0
31	3.2261602885477763E-4	0.0	0.0	18.45928263099824	0.0
32	3.2261602885477763E-4	0.0	0.0	18.707213049173134	0.0
33	3.2261602885477763E-4	0.0	0.0	18.962402327997264	0.0
34	3.2261602885477763E-4	0.0	0.0	19.205816121768194	0.0
35	3.2261602885477763E-4	0.0	0.0	19.48713729892956	0.0
36	3.2261602885477763E-4	0.0	0.0	19.733777252989036	0.0
37	3.2261602885477763E-4	0.0	0.0	20.000742016866365	0.0
38	3.2261602885477763E-4	0.0	0.0	20.299807075614744	0.0
39	3.2261602885477763E-4	0.0	0.0	20.658556099701258	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGTA	20	7.0307683E-4	45.000004	24
CCTCGTG	20	7.0307683E-4	45.000004	17
ATACGGA	20	7.0307683E-4	45.000004	28
CGGTCTA	35	1.2107193E-7	45.0	31
TTGTACG	30	2.163877E-6	44.999996	1
TATAGCG	30	2.163877E-6	44.999996	1
TACGGCT	1615	0.0	43.46749	7
CGTTTTT	2690	0.0	42.99257	1
TGATACC	1540	0.0	42.66234	4
CGATGAA	450	0.0	42.0	19
GATACCT	1565	0.0	41.98083	5
ACGGCTG	1855	0.0	41.603775	8
ACAACGA	105	0.0	40.714287	13
TTAGCGG	105	0.0	40.714287	2
CACAACG	105	0.0	40.714287	12
CGGCTGT	1895	0.0	40.369392	9
ATACCTG	1645	0.0	40.07599	6
ACCACCG	800	0.0	39.65625	14
CTACGAA	125	0.0	39.600002	11
CGGTAGT	205	0.0	39.512196	12
>>END_MODULE