##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936660.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	363705
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.079099819908993	31.0	31.0	33.0	28.0	34.0
2	31.10906091475234	31.0	31.0	34.0	28.0	34.0
3	30.861615870004538	31.0	30.0	34.0	27.0	34.0
4	34.84302387924279	35.0	35.0	37.0	32.0	37.0
5	35.05963624365901	35.0	35.0	37.0	33.0	37.0
6	35.0149984190484	35.0	35.0	37.0	32.0	37.0
7	30.955890075748204	35.0	35.0	37.0	0.0	37.0
8	32.84699962882006	35.0	33.0	37.0	17.0	37.0
9	35.89004550391114	38.0	34.0	39.0	32.0	39.0
10	35.93088079624971	37.0	35.0	39.0	32.0	39.0
11	36.21761042603209	37.0	35.0	39.0	32.0	39.0
12	36.275473254423225	37.0	35.0	39.0	32.0	39.0
13	36.03734895038561	38.0	35.0	39.0	31.0	39.0
14	37.10709228633095	39.0	36.0	40.0	31.0	41.0
15	37.16085838797927	39.0	36.0	40.0	31.0	41.0
16	37.347399678310715	39.0	36.0	40.0	32.0	41.0
17	37.07184393945643	39.0	35.0	40.0	31.0	41.0
18	36.99679960407473	39.0	35.0	40.0	31.0	41.0
19	36.75360525700774	38.0	35.0	40.0	31.0	41.0
20	36.57791616832323	38.0	35.0	40.0	30.0	41.0
21	36.80750608322679	39.0	35.0	40.0	31.0	41.0
22	36.85372210995175	39.0	35.0	40.0	31.0	41.0
23	36.95137542788799	39.0	35.0	40.0	32.0	41.0
24	36.84891326762074	39.0	35.0	40.0	31.0	41.0
25	36.65143454173025	38.0	35.0	40.0	31.0	41.0
26	36.50013060034918	38.0	35.0	40.0	31.0	41.0
27	36.55300861962304	38.0	35.0	40.0	31.0	41.0
28	36.48631720762706	38.0	35.0	40.0	31.0	41.0
29	36.390747996315696	38.0	35.0	40.0	30.0	41.0
30	36.246614701475096	38.0	35.0	40.0	30.0	41.0
31	36.296281326899546	38.0	35.0	40.0	30.0	41.0
32	36.04682916099586	38.0	35.0	40.0	30.0	41.0
33	35.80917776769635	38.0	35.0	40.0	29.0	41.0
34	35.64944391746058	38.0	35.0	40.0	27.0	41.0
35	35.58651104604006	38.0	35.0	40.0	27.0	41.0
36	35.464274618165824	38.0	35.0	40.0	26.0	41.0
37	35.44209180517177	38.0	35.0	40.0	26.0	41.0
38	35.35885401630442	38.0	35.0	40.0	26.0	41.0
39	35.247722742332385	38.0	35.0	40.0	25.0	41.0
40	35.10371867310045	38.0	34.0	40.0	24.0	41.0
41	34.8619073149943	38.0	34.0	40.0	24.0	41.0
42	34.93976161999423	38.0	34.0	40.0	24.0	41.0
43	34.91078758884261	38.0	34.0	40.0	24.0	41.0
44	34.79176530429881	38.0	34.0	40.0	23.0	41.0
45	34.710768342475355	38.0	34.0	40.0	23.0	41.0
46	34.641607896509534	38.0	34.0	40.0	23.0	41.0
47	34.551738359384665	37.0	34.0	40.0	23.0	41.0
48	34.46980107504709	37.0	34.0	40.0	23.0	41.0
49	34.4028072201372	37.0	33.0	40.0	23.0	41.0
50	34.196167223436575	37.0	33.0	40.0	23.0	41.0
51	32.482701640065436	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	17.0
10	33.0
11	13.0
12	18.0
13	15.0
14	17.0
15	28.0
16	55.0
17	111.0
18	250.0
19	585.0
20	1011.0
21	1473.0
22	2059.0
23	2811.0
24	3482.0
25	4481.0
26	5662.0
27	6204.0
28	6569.0
29	7220.0
30	8804.0
31	11247.0
32	14843.0
33	20578.0
34	29276.0
35	32174.0
36	36348.0
37	51619.0
38	65771.0
39	50916.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.65674104012868	17.068503319998353	19.362670295981633	14.91208534389134
2	32.01743170976478	31.22063210569005	21.35301961754719	15.408916566997979
3	29.278673650348498	30.91873908799714	25.614715222501754	14.18787203915261
4	25.167649606136845	22.6197055305811	35.236249157971436	16.976395705310622
5	29.802999683809684	25.832199172406206	25.441222969164567	18.92357817461954
6	23.119286234723198	37.54333869482135	26.969934424877305	12.367440645578148
7	61.4038850167031	17.10479646966635	17.640945271579987	3.850373242050563
8	69.51595386370822	12.244538843293329	13.233802119849877	5.005705173148568
9	64.72168378218612	7.086237472677031	14.096864216879066	14.09521452825779
10	39.21694780110254	22.55261819331601	22.145969948172283	16.084464057409164
11	31.6171072710026	23.390934961026105	27.134078442693944	17.857879325277352
12	26.676014902187212	21.175953038863916	30.90389189040569	21.244140168543186
13	23.944130545359563	23.235039386315833	36.31349582766253	16.507334240662075
14	20.950770542060187	28.910243191597583	29.729038643955953	20.409947622386273
15	17.87987517356099	23.63563877318156	39.91339134738318	18.571094705874266
16	21.73107325992219	25.01587825297975	29.728763695852408	23.524284791245652
17	22.72940982389574	23.952378988465927	31.71856312121087	21.599648066427463
18	22.93809543448674	23.991971515376473	31.631679520490508	21.43825352964628
19	22.38737438308519	26.247920704966937	28.78706644120922	22.57763847073865
20	23.50393863158329	26.32325648533839	32.81945532780688	17.353349555271443
21	22.862209757908193	29.162920498755863	30.394413054535956	17.580456688799988
22	21.398935950839277	22.202609257502644	32.07709544823415	24.32135934342393
23	20.274948103545455	28.70485695824913	31.239053628627595	19.78114130957782
24	22.262822892179102	24.534444123671655	29.504680991462862	23.69805199268638
25	20.235080628531364	31.091131548920142	28.359797088299583	20.31399073424891
26	19.76464442336509	25.290826356525205	30.79061327174496	24.153915948364745
27	23.250711428217922	26.663917185631213	27.960847390055125	22.12452399609574
28	18.360209510454904	25.18772081769566	33.690765867942424	22.761303803907012
29	22.072558804525645	26.371647351562395	29.76395705310623	21.791836790805736
30	23.282605408229195	26.475027838495485	30.428231671272048	19.814135082003272
31	24.02001622193811	23.71922299665938	27.806326555862583	24.454434225539927
32	25.38678324466257	26.523693652823027	28.81923536932404	19.27028773319036
33	22.226804690614646	22.860835017390468	27.617987104933945	27.294373187060945
34	21.790736998391555	26.32710575878803	31.302291692443053	20.579865550377367
35	25.549277573857935	24.067582243851472	28.212149956695676	22.17099022559492
36	23.04147592141983	27.1398523528684	27.781306278439942	22.037365447271828
37	23.072545057120468	24.659820458888383	32.632490617395966	19.635143866595183
38	21.42065685101937	25.519583178675028	25.735142491854663	27.32461747845094
39	22.215256870265737	23.71702341183102	32.6641096493037	21.40361006859955
40	25.159951059237567	23.125884989208288	28.7125555051484	23.00160844640574
41	19.875998405300997	25.214115835636026	31.12742469858814	23.782461060474837
42	23.462421467947927	23.629864863007107	31.717188380693145	21.190525288351825
43	23.082718136951648	23.173451011121653	29.881359893320138	23.862470958606565
44	22.516050095544465	23.33209606686738	30.665236936528228	23.486616901059925
45	20.740435242847912	23.65516008853329	28.67516256306622	26.929242105552575
46	25.304023865495388	25.78958221635666	27.392254711923126	21.514139206224826
47	19.560357982430816	24.081879545235836	35.1683369763957	21.18942549593764
48	22.686242971639103	25.511334735568663	27.899259014860945	23.90316327793129
49	21.447051868959736	22.376651407046918	33.20025845121734	22.976038272776016
50	21.903190772741645	23.620791575590108	30.28718329415323	24.188834357515017
51	21.655187583343643	23.90371317413838	27.065616364911122	27.37548287760685
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	301.0
2	517.0
3	2717.0
4	4917.0
5	3450.5
6	1984.0
7	1859.5
8	1735.0
9	1714.5
10	1694.0
11	1667.0
12	1640.0
13	1638.5
14	1637.0
15	1583.5
16	1530.0
17	1560.5
18	1591.0
19	1558.5
20	1526.0
21	1458.5
22	1391.0
23	1620.5
24	1850.0
25	1930.5
26	2394.5
27	2778.0
28	3241.0
29	3704.0
30	4580.5
31	5457.0
32	5958.0
33	6459.0
34	7832.5
35	9206.0
36	9876.5
37	10547.0
38	11631.5
39	12716.0
40	14258.0
41	15800.0
42	17416.5
43	19033.0
44	21587.0
45	24141.0
46	40628.0
47	57115.0
48	43192.5
49	29270.0
50	28701.0
51	28132.0
52	25453.0
53	22774.0
54	21621.0
55	20468.0
56	18626.0
57	16784.0
58	15303.5
59	13823.0
60	12769.0
61	11715.0
62	10531.0
63	9347.0
64	7929.0
65	6511.0
66	5558.0
67	4605.0
68	3871.5
69	3138.0
70	2699.0
71	2260.0
72	1890.0
73	1520.0
74	1262.5
75	822.0
76	639.0
77	488.5
78	338.0
79	241.5
80	145.0
81	116.0
82	87.0
83	57.0
84	27.0
85	20.0
86	13.0
87	14.0
88	15.0
89	12.5
90	10.0
91	12.0
92	14.0
93	7.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	363705.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.54522782387757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.17224221066698	25.968422331991963
2	8.119876226193954	5.610059482711147
3	3.638899665569481	3.771198539259888
4	2.1732579845287594	3.0030276878242788
5	1.469434289079629	2.5380971144236675
6	1.1076074658721746	2.2957531348789186
7	0.8082265201577026	1.9544258490503328
8	0.7270383529771449	2.009256443223375
9	0.6443008375258578	2.0031767297501295
>10	6.05877819415078	38.79517174784311
>50	0.05329369276827939	1.1947543153577402
>100	0.01670399325572936	1.1059995674597298
>500	0.0039771412513641335	1.157383895190157
>1k	0.0039771412513641335	2.372472115639082
>5k	0.00238628475081848	6.220801045396477
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG	8286	2.2782199859776466	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	7498	2.0615608803838272	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	6855	1.8847692498040993	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2667	0.7332865921557307	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	2033	0.5589694945079117	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT	1585	0.43579274411954744	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT	1175	0.32306402166591053	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTC	1174	0.32278907356236514	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	899	0.24717834508736475	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTA	895	0.24607855267318293	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG	842	0.2315063031852738	No Hit
GAATCTATCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	818	0.22490754870018284	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	758	0.2084106624874555	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTT	364	0.1000811096905459	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08853328934163676	0.0
2	0.0	0.0	0.0	0.8982554542830041	0.0
3	0.0	0.0	0.0	1.0439779491620957	0.0
4	0.0	0.0	0.0	1.5630799686559163	0.0
5	0.0	0.0	0.0	4.012042726935291	0.0
6	0.0	0.0	0.0	4.5847596266204755	0.0
7	0.0	0.0	0.0	5.313372101015934	0.0
8	0.0	0.0	0.0	6.1563629864863	0.0
9	0.0	0.0	0.0	6.352950880521302	0.0
10	0.0	0.0	0.0	9.360883133308588	0.0
11	0.0	0.0	0.0	9.9470724900675	0.0
12	0.0	0.0	0.0	12.79745947952324	0.0
13	0.0	0.0	0.0	13.122448137913969	0.0
14	0.0	0.0	0.0	13.307213263496514	0.0
15	0.0	0.0	0.0	13.760602686242972	0.0
16	0.0	0.0	0.0	14.167525879490245	0.0
17	0.0	0.0	0.0	14.58902132222543	0.0
18	0.0	0.0	0.0	15.052583824803069	0.0
19	2.749481035454558E-4	0.0	0.0	15.432287155799344	0.0
20	2.749481035454558E-4	0.0	0.0	15.716308546761798	0.0
21	2.749481035454558E-4	0.0	0.0	16.036073191185164	0.0
22	2.749481035454558E-4	0.0	0.0	16.378933476306347	0.0
23	2.749481035454558E-4	0.0	0.0	16.697873276419077	0.0
24	2.749481035454558E-4	0.0	0.0	16.960723663408533	0.0
25	2.749481035454558E-4	0.0	0.0	17.199103669182442	0.0
26	2.749481035454558E-4	0.0	0.0	17.42731059512517	0.0
27	2.749481035454558E-4	0.0	0.0	17.744875654720172	0.0
28	2.749481035454558E-4	0.0	0.0	17.99012936308272	0.0
29	2.749481035454558E-4	0.0	0.0	18.25820376403954	0.0
30	2.749481035454558E-4	0.0	0.0	18.56834522483881	0.0
31	2.749481035454558E-4	0.0	0.0	18.82377201303254	0.0
32	2.749481035454558E-4	0.0	0.0	19.086897348125543	0.0
33	2.749481035454558E-4	0.0	0.0	19.362120399774543	0.0
34	2.749481035454558E-4	0.0	0.0	19.647516531254727	0.0
35	2.749481035454558E-4	0.0	0.0	19.924664219628546	0.0
36	2.749481035454558E-4	0.0	0.0	20.173492253337184	0.0
37	2.749481035454558E-4	0.0	0.0	20.453114474642913	0.0
38	2.749481035454558E-4	0.0	0.0	20.77095448234146	0.0
39	2.749481035454558E-4	0.0	0.0	21.19794888714755	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTCGC	30	2.1613941E-6	45.000004	11
GGTACCC	20	7.02692E-4	45.0	31
TTGTGCG	35	1.208864E-7	45.0	1
TTCACGG	20	7.02692E-4	45.0	2
TACGGGT	35	1.208864E-7	45.0	4
CTATTCG	20	7.02692E-4	45.0	37
ATCGGGC	20	7.02692E-4	45.0	38
ATACGGT	20	7.02692E-4	45.0	28
CACCCGT	20	7.02692E-4	45.0	16
CTAGCGG	20	7.02692E-4	45.0	2
CGTTTTT	1435	0.0	42.96167	1
ACAACGA	85	0.0	42.35294	13
CGATGAA	240	0.0	42.187504	19
TACGGCT	805	0.0	41.366463	7
AGCTACG	60	3.6379788E-12	41.250004	9
ACGGCTG	950	0.0	41.21053	8
CGGCTGT	945	0.0	40.952377	9
CACCGAT	255	0.0	40.588234	16
TGATACC	910	0.0	40.549454	4
GATACCT	925	0.0	39.89189	5
>>END_MODULE