FastQCFastQC Report
Sat 14 Jan 2017
SRR2936659.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936659.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541916
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20830.38437691450335476No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC7500.13839783287446764No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC6120.11293263162556559No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5690.10499782254076277No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC207.0299814E-445.00000431
AACGGTT207.0299814E-445.00000418
CATAGCG207.0299814E-445.00000434
ATACGGT207.0299814E-445.00000441
CGCCGTT207.0299814E-445.00000415
CACTTCG207.0299814E-445.0000041
GTACGGA207.0299814E-445.00000418
TCCGCAA253.888068E-545.030
TACGATT253.888068E-545.013
TTACGAT253.888068E-545.012
CCTCGTC302.1633696E-644.99999636
CGTTTTT12200.040.3893431
AACGGGC451.9250365E-840.04
AACACGG451.9250365E-840.02
TAGACGG850.039.7058832
CTCACGT356.2421786E-638.5714345
ACCGGGT356.2421786E-638.571434
TTTACGA356.2421786E-638.5714311
CAACGCT356.2421786E-638.5714322
ACGATTT356.2421786E-638.5714314
Produced by FastQC (version 0.11.5)