##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936659.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541916
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2152713704707	31.0	31.0	33.0	28.0	34.0
2	31.245659843961057	31.0	31.0	34.0	28.0	34.0
3	31.048282390628806	31.0	31.0	34.0	28.0	34.0
4	35.02062496770717	35.0	35.0	37.0	32.0	37.0
5	35.238618162224405	35.0	35.0	37.0	33.0	37.0
6	35.21079281659888	37.0	35.0	37.0	33.0	37.0
7	30.899327571062674	35.0	35.0	37.0	0.0	37.0
8	33.027037400630356	36.0	35.0	37.0	17.0	37.0
9	36.407465363635694	39.0	35.0	39.0	32.0	39.0
10	36.17385904826578	37.0	35.0	39.0	32.0	39.0
11	36.39664080780047	38.0	35.0	39.0	32.0	39.0
12	36.43517814569048	38.0	35.0	39.0	32.0	39.0
13	36.397474885406595	39.0	35.0	39.0	32.0	39.0
14	37.46289646365857	39.0	36.0	41.0	32.0	41.0
15	37.68151152577152	39.0	36.0	41.0	33.0	41.0
16	37.581007019538085	39.0	36.0	41.0	33.0	41.0
17	37.49378501465172	39.0	36.0	41.0	32.0	41.0
18	37.41297913329741	39.0	36.0	40.0	32.0	41.0
19	37.236435167073864	39.0	36.0	40.0	32.0	41.0
20	37.23579300112932	39.0	35.0	40.0	32.0	41.0
21	37.213857867270946	39.0	35.0	40.0	32.0	41.0
22	37.287134906516876	39.0	35.0	40.0	32.0	41.0
23	37.35013729065021	39.0	35.0	41.0	32.0	41.0
24	37.288461680408034	39.0	35.0	41.0	32.0	41.0
25	36.957194472944146	39.0	35.0	40.0	31.0	41.0
26	36.91749643856243	39.0	35.0	40.0	31.0	41.0
27	36.938134323400675	39.0	35.0	40.0	31.0	41.0
28	36.94317938573506	39.0	35.0	40.0	31.0	41.0
29	36.94565393898685	39.0	35.0	40.0	31.0	41.0
30	36.72403472124831	39.0	35.0	40.0	31.0	41.0
31	36.72123539441537	39.0	35.0	40.0	31.0	41.0
32	36.580065914274535	39.0	35.0	40.0	31.0	41.0
33	36.45570346695798	39.0	35.0	40.0	30.0	41.0
34	36.36625971552787	39.0	35.0	40.0	30.0	41.0
35	36.29975125296171	39.0	35.0	40.0	30.0	41.0
36	36.257543604543876	39.0	35.0	40.0	30.0	41.0
37	36.20088168646063	39.0	35.0	40.0	30.0	41.0
38	36.10734320448188	38.0	35.0	40.0	30.0	41.0
39	36.05408218247847	38.0	35.0	40.0	30.0	41.0
40	35.965180950553226	38.0	35.0	40.0	29.0	41.0
41	35.819780187335304	38.0	35.0	40.0	29.0	41.0
42	35.82233408867795	38.0	35.0	40.0	29.0	41.0
43	35.777334494644926	38.0	35.0	40.0	29.0	41.0
44	35.59481358734564	38.0	35.0	40.0	28.0	41.0
45	35.53111921404793	38.0	34.0	40.0	28.0	41.0
46	35.44652861329062	38.0	34.0	40.0	28.0	41.0
47	35.42278508108268	38.0	34.0	40.0	28.0	41.0
48	35.334354401789206	38.0	34.0	40.0	27.0	41.0
49	35.199931354674895	38.0	34.0	40.0	27.0	41.0
50	35.06255397515482	37.0	34.0	40.0	27.0	41.0
51	33.48248621557585	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	18.0
9	52.0
10	61.0
11	43.0
12	27.0
13	29.0
14	21.0
15	50.0
16	87.0
17	165.0
18	283.0
19	462.0
20	797.0
21	1174.0
22	1796.0
23	2428.0
24	3451.0
25	4751.0
26	6174.0
27	7031.0
28	8280.0
29	9725.0
30	11691.0
31	14576.0
32	19228.0
33	27623.0
34	43723.0
35	46662.0
36	53607.0
37	80935.0
38	108049.0
39	88909.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.66881952184471	20.839761143793503	23.753312321466797	13.738107012894988
2	32.466101757467946	23.88303722348113	25.752884210837102	17.89797680821382
3	34.0903387240827	22.937318698838936	27.09478959838794	15.877552978690424
4	30.868437174765095	25.151684024830416	24.933199979332592	19.0466788210719
5	25.025280670805067	30.881723366721044	24.821743591257686	19.271252371216203
6	26.144642343093764	36.428523977885874	23.88451346703179	13.542320211988573
7	69.97671225798832	18.562470936455096	7.903623439795098	3.557193365761483
8	81.49196554447553	5.961440518456735	8.546158445220293	4.000435491847445
9	74.85274470582156	8.825722067626716	10.809608869271251	5.511924357280464
10	42.58944190612567	26.01141136264661	17.669159057861368	13.72998767336635
11	33.68012754744278	26.293373880822855	22.638194849386252	17.388303722348113
12	31.666715874785023	22.85815513843474	26.708198318558594	18.766930668221644
13	26.752301094634596	24.106134530074772	28.096605377955253	21.04495899733538
14	21.214727005661395	28.447397751681073	28.13498771027244	22.202887532385095
15	20.983325829095286	26.74067567667314	32.64491175754176	19.631086736689817
16	24.671904870865596	24.469290443537375	31.696425276242074	19.162379409354955
17	23.704596284294983	24.513577750057205	28.509584511252665	23.272241454395147
18	24.613408720170654	26.654684489847135	28.17724518191011	20.5546616080721
19	26.762819329933052	27.515519010326322	25.665232250016608	20.056429409724018
20	28.55645524398615	26.12951084669949	26.47864244643081	18.835391462883546
21	26.900848101919856	26.271968349338277	27.167494593257995	19.659688955483876
22	23.640933281172728	25.27568848308594	27.724961064076354	23.358417171664982
23	24.950361310609022	25.920806914724793	27.737324603813136	21.391507170853046
24	24.6442253042907	24.68980432391736	29.034389093512647	21.631581278279292
25	24.623926955469113	24.995571269348016	27.052532126750272	23.327969648432596
26	22.7730866038279	27.603170971146817	27.66443507849925	21.959307346526032
27	23.908686955173863	27.541722333350556	27.6946980712878	20.854892640187778
28	21.567918275157037	28.00526280825811	29.558455553997298	20.868363362587562
29	23.71345374559895	27.915581012555453	27.72053233342437	20.65043290842123
30	23.863846057322537	26.266247905579466	28.2840883088892	21.585817728208802
31	25.081931517061683	26.246134087201707	26.435462322573976	22.23647207316263
32	25.282331579063914	27.529543324057602	26.213656729087166	20.974468367791317
33	24.709180020519785	27.813904738003675	25.935753880675232	21.541161360801304
34	22.766628038293756	27.926468308741576	27.55685382974483	21.750049823219836
35	23.209685633935887	26.936462477579553	26.758944190612567	23.094907697871996
36	23.238287852729943	30.037681116630623	25.542704035311747	21.18132699532769
37	23.51139290960223	29.576724067936727	25.676857667978066	21.235025354482985
38	23.27943814170462	28.450350238782395	24.48718989658914	23.78302172292385
39	23.70164379719366	27.03001941260269	25.64862451007167	23.619712280131978
40	23.499398430753104	26.103492054119087	27.83531026948826	22.56179924563955
41	20.725167738173443	28.213413148901306	27.47787479978447	23.58354431314078
42	22.473224632599887	28.12077886609733	25.96620140390762	23.43979509739517
43	23.416359731028425	26.462403767373544	26.884793953306417	23.236442548291617
44	22.52212520021553	26.641213767447354	26.605230330899992	24.231430701437123
45	22.72732305375741	26.762450269045384	25.992035666044185	24.51819101115302
46	22.0801747872364	28.2065855224795	26.26550978380413	23.44772990647997
47	22.0881095963212	27.29721949527233	27.690638401523483	22.924032506882984
48	22.037363724267227	25.973951682548584	28.043091549243794	23.945593043940388
49	22.143099668583325	26.482886646638963	28.0323887835015	23.341624901276212
50	20.565548904258225	25.86286435536135	28.665512736291234	24.906074004089195
51	19.88703046228567	26.278611445316248	27.24924157987585	26.585116512522234
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	244.0
1	768.0
2	1292.0
3	2962.0
4	4632.0
5	3237.5
6	1843.0
7	1774.0
8	1705.0
9	1659.5
10	1614.0
11	1603.5
12	1593.0
13	1519.0
14	1445.0
15	1441.0
16	1437.0
17	1527.5
18	1618.0
19	1588.0
20	1558.0
21	1790.5
22	2023.0
23	2139.0
24	2255.0
25	2975.0
26	4289.0
27	4883.0
28	5830.5
29	6778.0
30	8044.0
31	9310.0
32	10206.0
33	11102.0
34	12838.5
35	14575.0
36	15178.0
37	15781.0
38	17958.5
39	20136.0
40	22473.0
41	24810.0
42	27171.5
43	29533.0
44	32421.0
45	35309.0
46	37553.5
47	39798.0
48	41510.5
49	43223.0
50	42580.5
51	41938.0
52	38522.0
53	35106.0
54	32906.5
55	30707.0
56	30174.0
57	29641.0
58	29065.0
59	28489.0
60	28923.5
61	29358.0
62	26036.5
63	22715.0
64	18580.0
65	14445.0
66	11887.0
67	9329.0
68	7708.0
69	6087.0
70	5235.0
71	4383.0
72	3699.0
73	3015.0
74	2500.0
75	1514.0
76	1043.0
77	773.5
78	504.0
79	428.5
80	353.0
81	280.5
82	208.0
83	173.5
84	139.0
85	127.0
86	115.0
87	99.0
88	83.0
89	69.0
90	55.0
91	36.0
92	17.0
93	12.5
94	8.0
95	4.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	541916.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.16211030309061
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19051807152066	22.807709972850734
2	8.748157306775761	5.452220858850687
3	3.615755428670097	3.380237084916487
4	2.190403738479813	2.730304116272399
5	1.5476124453293545	2.4113434863894563
6	1.1683639994861288	2.1845212695688105
7	0.8943661510983785	1.9509235656314794
8	0.6786948251533155	1.6919650402851527
9	0.567439647075874	1.5914355185272673
>10	7.167112327089735	49.46547648941753
>50	0.19790300987887824	3.7834547491572716
>100	0.03131002842859864	1.8114633481757272
>500	0.0017722657601093568	0.3554812728991188
>1k	5.907552533697856E-4	0.38346322705793084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2083	0.38437691450335476	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	750	0.13839783287446764	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	612	0.11293263162556559	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	569	0.10499782254076277	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08063980395485647	0.0
2	0.0	0.0	0.0	0.4026454284427845	0.0
3	0.0	0.0	0.0	0.5118874511916977	0.0
4	0.0	0.0	0.0	0.6715062851069169	0.0
5	0.0	0.0	0.0	1.149809195521077	0.0
6	0.0	0.0	0.0	1.4867617859594475	0.0
7	0.0	0.0	0.0	1.7792425394341558	0.0
8	0.0	0.0	0.0	2.395574221835118	0.0
9	0.0	0.0	0.0	2.619778711091756	0.0
10	0.0	0.0	0.0	3.138309258261428	0.0
11	0.0	0.0	0.0	3.8046486909410313	0.0
12	0.0	0.0	0.0	4.372633397057847	0.0
13	0.0	0.0	0.0	4.612338443596425	0.0
14	0.0	0.0	0.0	4.713830187704367	0.0
15	0.0	0.0	0.0	4.898545161980824	0.0
16	0.0	0.0	0.0	5.3039585470810975	0.0
17	0.0	0.0	0.0	5.742402881627411	0.0
18	0.0	0.0	0.0	6.2720052554270405	0.0
19	0.0	0.0	0.0	6.55895009558677	0.0
20	1.845304438326235E-4	0.0	0.0	6.891843016260823	0.0
21	1.845304438326235E-4	0.0	0.0	7.248724894633116	0.0
22	1.845304438326235E-4	0.0	0.0	7.655245462396386	0.0
23	1.845304438326235E-4	0.0	0.0	8.039068785568244	0.0
24	1.845304438326235E-4	0.0	0.0	8.354800374965862	0.0
25	1.845304438326235E-4	0.0	0.0	8.62975073627647	0.0
26	1.845304438326235E-4	0.0	0.0	8.90045689737893	0.0
27	1.845304438326235E-4	0.0	0.0	9.151602831435131	0.0
28	1.845304438326235E-4	0.0	0.0	9.443529993578341	0.0
29	1.845304438326235E-4	0.0	0.0	9.725123450866924	0.0
30	1.845304438326235E-4	0.0	0.0	10.059308084647805	0.0
31	1.845304438326235E-4	0.0	0.0	10.373563430494762	0.0
32	1.845304438326235E-4	0.0	0.0	10.701474029185334	0.0
33	1.845304438326235E-4	0.0	0.0	11.024955897223924	0.0
34	1.845304438326235E-4	0.0	0.0	11.327401294665593	0.0
35	1.845304438326235E-4	0.0	0.0	11.64996051048502	0.0
36	3.69060887665247E-4	0.0	0.0	11.989311996693214	0.0
37	3.69060887665247E-4	0.0	0.0	12.307996073192156	0.0
38	3.69060887665247E-4	0.0	0.0	12.653990655378324	0.0
39	3.69060887665247E-4	0.0	0.0	13.008289107536962	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTCGC	20	7.0299814E-4	45.000004	31
AACGGTT	20	7.0299814E-4	45.000004	18
CATAGCG	20	7.0299814E-4	45.000004	34
ATACGGT	20	7.0299814E-4	45.000004	41
CGCCGTT	20	7.0299814E-4	45.000004	15
CACTTCG	20	7.0299814E-4	45.000004	1
GTACGGA	20	7.0299814E-4	45.000004	18
TCCGCAA	25	3.888068E-5	45.0	30
TACGATT	25	3.888068E-5	45.0	13
TTACGAT	25	3.888068E-5	45.0	12
CCTCGTC	30	2.1633696E-6	44.999996	36
CGTTTTT	1220	0.0	40.389343	1
AACGGGC	45	1.9250365E-8	40.0	4
AACACGG	45	1.9250365E-8	40.0	2
TAGACGG	85	0.0	39.705883	2
CTCACGT	35	6.2421786E-6	38.57143	45
ACCGGGT	35	6.2421786E-6	38.57143	4
TTTACGA	35	6.2421786E-6	38.57143	11
CAACGCT	35	6.2421786E-6	38.57143	22
ACGATTT	35	6.2421786E-6	38.57143	14
>>END_MODULE