##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936657.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	212783
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10578852633904	31.0	31.0	33.0	28.0	34.0
2	31.145552041281494	31.0	31.0	34.0	28.0	34.0
3	30.870135302162296	31.0	31.0	34.0	27.0	34.0
4	34.81611312933834	35.0	35.0	37.0	32.0	37.0
5	35.12320063162941	35.0	35.0	37.0	33.0	37.0
6	35.1247233096629	36.0	35.0	37.0	32.0	37.0
7	30.95506689914138	35.0	35.0	37.0	0.0	37.0
8	33.040228777674905	35.0	35.0	37.0	17.0	37.0
9	36.25570651790792	39.0	35.0	39.0	32.0	39.0
10	36.17398946344398	37.0	35.0	39.0	32.0	39.0
11	36.41924401855412	38.0	35.0	39.0	32.0	39.0
12	36.3931094119361	38.0	35.0	39.0	32.0	39.0
13	36.20765286700535	38.0	35.0	39.0	32.0	39.0
14	37.26120507747329	39.0	36.0	40.0	32.0	41.0
15	37.517367458866545	39.0	36.0	41.0	32.0	41.0
16	37.46843497835823	39.0	36.0	41.0	32.0	41.0
17	37.35053552210468	39.0	35.0	40.0	32.0	41.0
18	37.22523415874389	39.0	36.0	40.0	32.0	41.0
19	36.943642114266645	38.0	35.0	40.0	32.0	41.0
20	36.841542792422324	38.0	35.0	40.0	32.0	41.0
21	36.8640492896519	38.0	35.0	40.0	32.0	41.0
22	36.90432036393885	38.0	35.0	40.0	32.0	41.0
23	36.97758279561807	39.0	35.0	40.0	32.0	41.0
24	36.876940357077395	38.0	35.0	40.0	32.0	41.0
25	36.593694045106986	38.0	35.0	40.0	31.0	41.0
26	36.52490565505703	38.0	35.0	40.0	31.0	41.0
27	36.535879276069984	38.0	35.0	40.0	31.0	41.0
28	36.463537970608556	38.0	35.0	40.0	31.0	41.0
29	36.45825089410338	38.0	35.0	40.0	31.0	41.0
30	36.23031915143597	38.0	35.0	40.0	30.0	41.0
31	36.06972831476199	38.0	35.0	40.0	30.0	41.0
32	35.772472424958764	38.0	35.0	40.0	29.0	41.0
33	35.5302867240334	38.0	35.0	40.0	27.0	41.0
34	35.338758265462936	38.0	34.0	40.0	26.0	41.0
35	35.19549494085524	38.0	34.0	40.0	25.0	41.0
36	35.0609165205867	38.0	34.0	40.0	24.0	41.0
37	34.914104980191084	38.0	34.0	40.0	24.0	41.0
38	34.75350474426998	38.0	34.0	40.0	23.0	41.0
39	34.78352593957224	38.0	34.0	40.0	23.0	41.0
40	34.662844306171074	38.0	34.0	40.0	23.0	41.0
41	34.507319663694936	38.0	34.0	40.0	23.0	41.0
42	34.45102287306787	38.0	34.0	40.0	23.0	41.0
43	34.42025443761955	37.0	34.0	40.0	23.0	41.0
44	34.16640897064145	37.0	33.0	40.0	22.0	41.0
45	34.1247938040163	37.0	33.0	40.0	22.0	41.0
46	33.9981483483173	37.0	33.0	40.0	21.0	41.0
47	33.92470263131923	37.0	33.0	40.0	21.0	41.0
48	33.85950005404567	37.0	33.0	40.0	20.0	41.0
49	33.736304122039826	36.0	33.0	40.0	20.0	41.0
50	33.59681929477449	36.0	33.0	40.0	20.0	41.0
51	31.93057246114586	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	10.0
10	21.0
11	10.0
12	5.0
13	11.0
14	7.0
15	19.0
16	32.0
17	69.0
18	118.0
19	245.0
20	433.0
21	702.0
22	1003.0
23	1494.0
24	2149.0
25	3079.0
26	4083.0
27	4532.0
28	4582.0
29	4756.0
30	5327.0
31	6604.0
32	8538.0
33	11512.0
34	18240.0
35	19923.0
36	20012.0
37	29230.0
38	37039.0
39	28986.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.80338184911389	19.672624222799755	22.18269316627738	19.34130076180898
2	34.99856661481415	24.955001104411537	23.693622140866516	16.352810139907795
3	30.2213992659188	22.549733766325318	30.97192914847521	16.256937819280676
4	27.837280233853267	24.092620181123493	30.1875619762857	17.88253760873754
5	25.024085570745786	28.38619626567912	28.543163692588223	18.046554470986877
6	23.438902543906234	34.834079790208804	28.780964644731956	12.946053021153006
7	63.92568955226686	18.16169524821062	14.33056212197403	3.5820530775484882
8	74.45096647758514	6.9154960687648925	14.058453917841183	4.575083535808782
9	68.14360169750402	7.761898271948417	16.368788860012312	7.725711170535241
10	39.96277898140359	23.787144649713557	23.191232382286177	13.058843986596674
11	31.791543497365858	24.537674532269964	27.036934341559242	16.633847628804933
12	29.76130611937984	20.665654681060047	32.04814294375021	17.52489625580991
13	22.81244272333786	27.04210392747541	32.964099575624	17.181353773562737
14	17.961021322192092	30.42489296607342	32.08855970636752	19.52552600536697
15	17.68327356978706	24.324311622639026	39.742836598788436	18.24957820878548
16	18.875568066997833	23.947871775470787	36.68103184934887	20.495528308182514
17	20.0584633170883	23.21003087652679	33.1088479812767	23.62265782510821
18	21.837270834606148	24.063482515050545	33.58069018671605	20.518556463627263
19	23.973719705051625	25.406164966186207	30.22844870125903	20.391666627503138
20	24.676313427294474	23.922023845889946	32.26855528872137	19.133107438094207
21	23.271125982808776	26.011946443089904	31.703190574434988	19.013736999666328
22	20.737088959174372	23.70631112447893	31.60637832909584	23.950221587250862
23	20.196162287400778	26.61349825878947	30.951720767166552	22.2386186866432
24	20.511037065931017	23.96714023206741	33.94867071147601	21.573151990525556
25	19.618578551857997	25.64114614419385	32.61585746981667	22.124417834131485
26	19.660875163899373	26.730048923081263	31.57724066302289	22.031835249996476
27	21.29305442634045	25.911844461258653	32.4969569937448	20.298144118656094
28	17.93235361847516	25.644905843041972	35.998176546058666	20.424563992424208
29	20.780325495927777	23.65367534060522	32.604578373272304	22.961420790194705
30	21.47164012162626	24.913174454726175	32.943891194315334	20.67129422933223
31	24.644355987085433	25.136406573833437	29.400844992316117	20.818392446765014
32	23.779625252017315	26.3376303558085	29.248107226611147	20.63463716556304
33	22.39417622648426	25.411804514458392	29.780574575976466	22.413444683080886
34	22.530935272084708	25.520365818697922	30.3966952247125	21.55200368450487
35	22.612708722031368	25.166484164618414	29.34021984839014	22.880587264960077
36	25.709760648172082	26.295803706123138	29.133436411743418	18.86099923396136
37	21.92891349402913	28.368337696150537	29.859998214143047	19.842750595677288
38	19.440462818928204	28.813862009653025	29.31672173058938	22.42895344082939
39	22.43600287616962	25.837590409008236	29.741097738071183	21.98530897675096
40	23.256557149772302	26.220609729160692	30.513715851360306	20.009117269706696
41	21.11399876869863	26.461700417796536	28.83360042860567	23.59070038489917
42	21.703801525497806	26.19241198779978	29.842609606970484	22.261176879731934
43	23.26360658511253	24.91505430415024	29.110878218654683	22.710460892082544
44	21.372008102151018	26.108758688429056	29.377346874515354	23.141886334904573
45	21.608399167226704	25.946151713247765	27.845739556261545	24.599709563263982
46	22.75369742883595	26.824511356640336	28.733968409130426	21.68782280539329
47	20.688682836504796	26.074921398795958	31.959790020819334	21.276605743879916
48	21.65163570398011	25.13781646090148	30.368497483351582	22.842050351766822
49	20.731449410902187	23.91356452348167	32.900184695205915	22.45480137041023
50	20.923194052156422	24.55224336530644	31.95415047254715	22.57041210998999
51	20.20791134630116	24.370367933528524	29.16445392724043	26.257266792929883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110.0
1	295.5
2	481.0
3	2980.0
4	5479.0
5	3740.0
6	2001.0
7	1843.5
8	1686.0
9	1621.5
10	1557.0
11	1528.0
12	1499.0
13	1399.0
14	1299.0
15	1237.5
16	1176.0
17	1046.0
18	916.0
19	905.0
20	894.0
21	997.5
22	1101.0
23	1097.0
24	1093.0
25	1343.0
26	1580.0
27	1567.0
28	1973.0
29	2379.0
30	2962.5
31	3546.0
32	3755.0
33	3964.0
34	4513.0
35	5062.0
36	5323.0
37	5584.0
38	6044.5
39	6505.0
40	8076.5
41	9648.0
42	11130.0
43	12612.0
44	13860.5
45	15109.0
46	17880.0
47	20651.0
48	20151.5
49	19652.0
50	18585.5
51	17519.0
52	15402.0
53	13285.0
54	12176.0
55	11067.0
56	10139.0
57	9211.0
58	8505.0
59	7799.0
60	7569.0
61	7339.0
62	6793.0
63	6247.0
64	5313.5
65	4380.0
66	3631.0
67	2882.0
68	2335.0
69	1788.0
70	1733.5
71	1679.0
72	1315.0
73	951.0
74	797.0
75	522.0
76	401.0
77	316.5
78	232.0
79	162.5
80	93.0
81	76.0
82	59.0
83	43.0
84	27.0
85	18.5
86	10.0
87	7.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	212783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.6905109900697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.76654322373096	28.933702410436922
2	8.46519283282834	6.381148869975515
3	3.4900684547188865	3.946273903460333
4	1.884063392311625	2.8404524797563715
5	1.2568735271013356	2.368610274317027
6	0.8753226349455729	1.9794814435363728
7	0.6346712552525593	1.6744758744824542
8	0.5436476764049427	1.6392286977813075
9	0.44264891083429964	1.5015297274688297
>10	5.528747241237422	41.835578970124494
>50	0.07481390042269855	1.8446022473599863
>100	0.03241935684983603	2.3869388062016235
>500	0.0037406950211349268	1.2021637066870945
>1k	0.001246898340378309	1.4658125884116682
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3119	1.4658125884116682	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG	908	0.42672581926187714	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC	828	0.38912883078065447	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC	822	0.3863090566445628	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	482	0.2265218555993665	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	482	0.2265218555993665	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	480	0.2255819308873359	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	304	0.14286855622864608	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	264	0.12407006198803475	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	260	0.12219021256397362	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	245	0.11514077722374438	No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT	222	0.10433164303539286	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6996235601528316E-4	0.0	0.0	0.12547994905608062	0.0
2	4.6996235601528316E-4	0.0	0.0	0.8125649135504246	0.0
3	4.6996235601528316E-4	0.0	0.0	1.007129328940752	0.0
4	4.6996235601528316E-4	0.0	0.0	1.3638307571563517	0.0
5	4.6996235601528316E-4	0.0	0.0	2.520878077665979	0.0
6	4.6996235601528316E-4	0.0	0.0	3.0660344106437076	0.0
7	4.6996235601528316E-4	0.0	0.0	3.6572470545109335	0.0
8	4.6996235601528316E-4	0.0	0.0	4.6648463458077005	0.0
9	4.6996235601528316E-4	0.0	0.0	5.057264913080462	0.0
10	4.6996235601528316E-4	0.0	0.0	6.342611956782262	0.0
11	4.6996235601528316E-4	0.0	0.0	7.386398349492206	0.0
12	4.6996235601528316E-4	0.0	0.0	8.69430358628274	0.0
13	4.6996235601528316E-4	0.0	0.0	9.074973094655117	0.0
14	4.6996235601528316E-4	0.0	0.0	9.224421123867979	0.0
15	4.6996235601528316E-4	0.0	0.0	9.55997424606289	0.0
16	4.6996235601528316E-4	0.0	0.0	10.201942824379767	0.0
17	4.6996235601528316E-4	0.0	0.0	10.906886358402692	0.0
18	4.6996235601528316E-4	0.0	0.0	11.781486302947133	0.0
19	4.6996235601528316E-4	0.0	0.0	12.190823515036445	0.0
20	4.6996235601528316E-4	0.0	0.0	12.63869764031901	0.0
21	4.6996235601528316E-4	0.0	0.0	13.148136834239578	0.0
22	4.6996235601528316E-4	0.0	0.0	13.650526592819915	0.0
23	4.6996235601528316E-4	0.0	0.0	14.135527744227687	0.0
24	4.6996235601528316E-4	0.0	0.0	14.54580488102903	0.0
25	4.6996235601528316E-4	0.0	0.0	14.911905556364935	0.0
26	4.6996235601528316E-4	0.0	0.0	15.26014766217226	0.0
27	4.6996235601528316E-4	0.0	0.0	15.606979880911538	0.0
28	4.6996235601528316E-4	0.0	0.0	15.938773304258328	0.0
29	4.6996235601528316E-4	0.0	0.0	16.270566727605118	0.0
30	4.6996235601528316E-4	0.0	0.0	16.703402057495193	0.0
31	4.6996235601528316E-4	0.0	0.0	17.100990210684124	0.0
32	4.6996235601528316E-4	0.0	0.0	17.55356395952684	0.0
33	4.6996235601528316E-4	0.0	0.0	17.919194672506734	0.0
34	4.6996235601528316E-4	0.0	0.0	18.24910824642946	0.0
35	4.6996235601528316E-4	0.0	0.0	18.650926060822528	0.0
36	4.6996235601528316E-4	0.0	0.0	19.033945380974984	0.0
37	4.6996235601528316E-4	0.0	0.0	19.39487647039472	0.0
38	4.6996235601528316E-4	0.0	0.0	19.77272620463101	0.0
39	4.6996235601528316E-4	0.0	0.0	20.14211661645902	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAT	20	7.020316E-4	45.000004	16
CGGAACG	20	7.020316E-4	45.000004	42
ATTAGCG	20	7.020316E-4	45.000004	13
CGCGGAA	20	7.020316E-4	45.000004	40
TTAGCGT	20	7.020316E-4	45.000004	14
GCGCGCC	20	7.020316E-4	45.000004	16
CCGTGGT	20	7.020316E-4	45.000004	38
ATTAACG	20	7.020316E-4	45.000004	1
CACGACC	30	2.1571395E-6	45.000004	27
TCGATTT	20	7.020316E-4	45.000004	15
GAATAAC	20	7.020316E-4	45.000004	9
TAACGCC	20	7.020316E-4	45.000004	12
ACGGGTC	30	2.1571395E-6	45.000004	5
GACCCAC	20	7.020316E-4	45.000004	12
AGAACCG	20	7.020316E-4	45.000004	2
ACGATGG	30	2.1571395E-6	45.000004	2
ACGATCT	20	7.020316E-4	45.000004	16
CCATCGA	20	7.020316E-4	45.000004	31
CGTAGGT	30	2.1571395E-6	45.000004	3
AGACCTA	20	7.020316E-4	45.000004	32
>>END_MODULE