##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936655.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	229283
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01900271716612	31.0	31.0	33.0	28.0	34.0
2	31.055795676085886	31.0	31.0	34.0	28.0	34.0
3	30.72099981245884	31.0	30.0	34.0	27.0	34.0
4	34.71407387377172	35.0	35.0	37.0	32.0	37.0
5	35.06401259578774	35.0	35.0	37.0	33.0	37.0
6	35.07233855104827	35.0	35.0	37.0	32.0	37.0
7	31.253769359263444	35.0	35.0	37.0	0.0	37.0
8	33.191148057204416	36.0	35.0	37.0	17.0	37.0
9	36.368583802549686	39.0	35.0	39.0	32.0	39.0
10	36.171255609879495	37.0	35.0	39.0	32.0	39.0
11	36.42196761207765	38.0	35.0	39.0	32.0	39.0
12	36.37378261798738	38.0	35.0	39.0	32.0	39.0
13	36.149343824007886	38.0	35.0	39.0	32.0	39.0
14	37.19463719508206	39.0	36.0	40.0	32.0	41.0
15	37.464378082980424	39.0	36.0	41.0	32.0	41.0
16	37.45220535320979	39.0	36.0	41.0	32.0	41.0
17	37.3707906822573	39.0	36.0	40.0	32.0	41.0
18	37.214429329693	39.0	36.0	40.0	32.0	41.0
19	36.93026521809293	38.0	35.0	40.0	32.0	41.0
20	36.79701940396802	38.0	35.0	40.0	32.0	41.0
21	36.783656005896646	38.0	35.0	40.0	32.0	41.0
22	36.82750574617394	38.0	35.0	40.0	32.0	41.0
23	36.90639079216514	38.0	35.0	40.0	32.0	41.0
24	36.7858672470266	38.0	35.0	40.0	32.0	41.0
25	36.462712019643845	38.0	35.0	40.0	31.0	41.0
26	36.45094926357384	38.0	35.0	40.0	31.0	41.0
27	36.502963586484825	38.0	35.0	40.0	31.0	41.0
28	36.48425308461596	38.0	35.0	40.0	31.0	41.0
29	36.46556438985882	38.0	35.0	40.0	31.0	41.0
30	36.200346296934356	38.0	35.0	40.0	30.0	41.0
31	36.08371314052939	38.0	35.0	40.0	30.0	41.0
32	35.72391760400902	38.0	35.0	40.0	29.0	41.0
33	35.44551056990706	38.0	35.0	40.0	27.0	41.0
34	35.23267752079308	38.0	34.0	40.0	25.0	41.0
35	35.059720956198234	38.0	34.0	40.0	24.0	41.0
36	34.91795292280719	38.0	34.0	40.0	23.0	41.0
37	34.805275576471	38.0	34.0	40.0	23.0	41.0
38	34.65530806906749	38.0	34.0	40.0	23.0	41.0
39	34.63000309660987	38.0	34.0	40.0	23.0	41.0
40	34.51530205030465	38.0	34.0	40.0	22.0	41.0
41	34.407055909073065	38.0	34.0	40.0	22.0	41.0
42	34.39764832107047	38.0	34.0	40.0	22.0	41.0
43	34.28088432199509	38.0	33.0	40.0	21.0	41.0
44	34.04108895993161	37.0	33.0	40.0	20.0	41.0
45	33.95077698739113	37.0	33.0	40.0	20.0	41.0
46	33.850429382030065	37.0	33.0	40.0	20.0	41.0
47	33.838391856352196	37.0	33.0	40.0	20.0	41.0
48	33.772163658012154	37.0	33.0	40.0	20.0	41.0
49	33.65963023861342	37.0	33.0	40.0	20.0	41.0
50	33.47474954532172	36.0	33.0	40.0	18.0	41.0
51	31.95173650030748	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	26.0
10	24.0
11	19.0
12	5.0
13	9.0
14	14.0
15	14.0
16	29.0
17	70.0
18	153.0
19	308.0
20	480.0
21	796.0
22	1136.0
23	1633.0
24	2480.0
25	3751.0
26	4709.0
27	5160.0
28	5190.0
29	5252.0
30	5750.0
31	6771.0
32	8851.0
33	12062.0
34	19392.0
35	21235.0
36	21238.0
37	30899.0
38	40424.0
39	31394.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.332837584993214	17.32618641591396	21.880819772944353	18.46015622614847
2	35.33188243349921	24.896743325933453	23.633675414226087	16.137698826341246
3	30.02272301042816	23.310494018309253	32.14237427109729	14.524408700165298
4	27.41372016241937	24.5020346035249	31.2447935520732	16.839451681982528
5	24.74496582825591	27.667991085252723	29.431750282402096	18.15529280408927
6	23.60968759131728	34.10545046950711	29.257293388519862	13.02756855065574
7	63.34006446182229	17.62974141126904	15.196067741611893	3.834126385296773
8	72.87631442365985	7.164944631743304	15.301614162410646	4.657126782186206
9	66.9111970795916	8.720663982938117	17.546438244440278	6.821700693030011
10	38.77522537649978	24.84745925341172	24.008757736072887	12.368557634015605
11	33.030796003192556	23.455293240231505	27.753474963255016	15.760435793320918
12	28.2519855375235	22.022565999223666	32.940514560608506	16.78493390264433
13	21.71334115481741	26.83408713249565	34.69118949071671	16.761382221970226
14	17.727437271843094	30.121291155471624	33.16818080712482	18.983090765560465
15	17.36456693256805	24.985280199578686	40.64322256774379	17.00693030010947
16	18.657292516235394	23.009119734127694	38.97585080446435	19.35773694517256
17	18.341089396073848	23.04401111290414	34.03174243184187	24.583157059180138
18	20.72591513544397	24.535181413362526	35.046645412001766	19.69225803919174
19	23.38071291809685	25.041106405621	31.71800787672876	19.860172799553393
20	24.112559587932818	25.035000414335123	32.59290919954816	18.259530798183903
21	22.145994251645345	26.795270473606852	32.220879873344295	18.837855401403505
22	19.945656677555686	23.622771858358448	32.37047665984831	24.061094804237555
23	19.555309377494186	26.096134471373805	33.07746322230606	21.27109292882595
24	20.34777981795423	23.907572737621194	35.382911074959765	20.36173636946481
25	18.078531770781087	26.79745118478038	34.281651932328174	20.84236511211036
26	18.45928394167906	27.296397901283566	33.46170453108168	20.782613625955694
27	20.745977678240425	27.002438035092002	32.781758787175676	19.469825499491893
28	16.609604724292687	26.724615431584546	36.98268079185984	19.68309905226292
29	18.671249067745972	24.63549412734481	36.07376037473341	20.61949643017581
30	18.302708879419757	24.134802841902804	35.92721658387234	21.635271694805112
31	23.390308047260373	24.786399340552943	31.2993113314114	20.523981280775285
32	22.775783638560206	27.0800713528696	31.029775430363348	19.114369578206844
33	21.463867796565815	26.554083817814668	30.17231979693217	21.809728588687342
34	19.55574551972889	27.115398873880753	32.07695293589145	21.251902670498904
35	20.591583327154652	24.79643061195117	31.248718832185553	23.363267228708626
36	22.59958217573915	26.58112463636641	30.980491357841622	19.838801830052816
37	20.10397630875381	28.018213299721307	31.661309386217035	20.21650100530785
38	19.66957864298705	29.08676177474998	29.91107059834353	21.332588983919436
39	21.594274324742784	26.346916256329518	30.527775718217224	21.531033700710474
40	21.313834867827094	24.87755306760641	32.76213238661392	21.046479677952572
41	18.88888404286406	25.82616242809105	30.379923500652033	24.90503002839286
42	22.230169702943524	24.56745593873074	29.560848383874948	23.64152597445079
43	23.490620761242656	24.853565244697602	29.04358369351413	22.612230300545612
44	20.182481911000817	27.25060296663948	30.169266801289236	22.397648321070466
45	19.76945521473463	27.254092104517124	29.254240392876923	23.72221228787132
46	21.08311562566784	27.308173741620617	30.03929641534697	21.569414217364567
47	20.589402615981122	24.479791349554915	33.44862026404051	21.482185770423452
48	20.654387808952254	24.381659346746158	31.972278799562115	22.991674044739472
49	20.412328868690658	23.850001962640054	32.907803893005585	22.8298652756637
50	18.936859688681672	25.41444415852898	32.85721139377974	22.79148475900961
51	19.531757696820087	24.315801869305616	29.70303075238897	26.449409681485324
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	99.0
1	300.0
2	501.0
3	3397.0
4	6293.0
5	4414.5
6	2536.0
7	2336.5
8	2137.0
9	2033.5
10	1930.0
11	1854.5
12	1779.0
13	1732.0
14	1685.0
15	1537.5
16	1390.0
17	1321.0
18	1252.0
19	1277.5
20	1303.0
21	1201.5
22	1100.0
23	1150.5
24	1201.0
25	1476.5
26	1849.0
27	1946.0
28	2677.5
29	3409.0
30	3815.0
31	4221.0
32	4882.0
33	5543.0
34	5969.0
35	6395.0
36	6561.0
37	6727.0
38	7150.0
39	7573.0
40	9439.5
41	11306.0
42	12687.5
43	14069.0
44	15623.0
45	17177.0
46	19765.5
47	22354.0
48	21348.0
49	20342.0
50	19466.5
51	18591.0
52	16050.0
53	13509.0
54	11908.5
55	10308.0
56	9811.5
57	9315.0
58	8604.0
59	7893.0
60	7268.5
61	6644.0
62	6222.5
63	5801.0
64	4767.5
65	3734.0
66	3052.0
67	2370.0
68	1966.5
69	1563.0
70	1489.5
71	1416.0
72	1063.5
73	711.0
74	680.0
75	497.0
76	345.0
77	269.5
78	194.0
79	167.0
80	140.0
81	91.0
82	42.0
83	31.5
84	21.0
85	14.0
86	7.0
87	4.5
88	2.0
89	3.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	229283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.844423703458176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.28994531120529	28.47703493063158
2	8.62828191955302	6.358081497537977
3	3.340514690215204	3.6923801590174588
4	1.90700537418026	2.8105005604427715
5	1.2228035701602784	2.2526746422543322
6	0.8593953455337484	1.8998355743775162
7	0.6179123558796373	1.5936637256141972
8	0.4569236961102298	1.3468072207708377
9	0.37406188593479955	1.2403885155026757
>10	5.109022467387959	41.64155214298487
>50	0.15625369918795426	3.589014449392236
>100	0.031960983924808825	2.1017694290462003
>500	0.00473496058145316	1.4375248055895988
>1k	0.00118374014536329	1.5587723468377506
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3574	1.5587723468377506	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	986	0.43003624341970403	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	826	0.3602534858668109	No Hit
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	807	0.35196678340740484	TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	677	0.29526829289567913	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	445	0.1940832944439841	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	351	0.15308592438165936	No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	291	0.12691739029932442	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	290	0.12648124806461883	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	255	0.11121626984992347	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14523536415695887	0.0
2	0.0	0.0	0.0	0.8884217320952709	0.0
3	0.0	0.0	0.0	1.087738733355722	0.0
4	0.0	0.0	0.0	1.4436307968754771	0.0
5	0.0	0.0	0.0	2.4947335825159302	0.0
6	0.0	0.0	0.0	2.9962971524273496	0.0
7	0.0	0.0	0.0	3.541038803574622	0.0
8	0.0	0.0	0.0	4.556814068203923	0.0
9	0.0	0.0	0.0	4.889154451049576	0.0
10	0.0	0.0	0.0	6.020943550110562	0.0
11	0.0	0.0	0.0	6.972605906238142	0.0
12	0.0	0.0	0.0	8.265767632140193	0.0
13	0.0	0.0	0.0	8.703654435784598	0.0
14	0.0	0.0	0.0	8.902535294810344	0.0
15	0.0	0.0	0.0	9.258863500564804	0.0
16	0.0	0.0	0.0	9.842421810600873	0.0
17	0.0	0.0	0.0	10.52105912780276	0.0
18	0.0	0.0	0.0	11.40860857542862	0.0
19	0.0	0.0	0.0	11.857835077175368	0.0
20	0.0	0.0	0.0	12.281329187074489	0.0
21	0.0	0.0	0.0	12.749745947148284	0.0
22	0.0	0.0	0.0	13.287509322540267	0.0
23	0.0	0.0	0.0	13.832687115922244	0.0
24	0.0	0.0	0.0	14.257925794760187	0.0
25	0.0	0.0	0.0	14.639986392362276	0.0
26	0.0	0.0	0.0	14.961423219340292	0.0
27	0.0	0.0	0.0	15.288966037604183	0.0
28	0.0	0.0	0.0	15.645294243358643	0.0
29	0.0	0.0	0.0	16.016451285093094	0.0
30	0.0	0.0	0.0	16.480506622819835	0.0
31	0.0	0.0	0.0	16.87477920299368	0.0
32	0.0	0.0	0.0	17.235468831095197	0.0
33	0.0	0.0	0.0	17.578276627573786	0.0
34	0.0	0.0	0.0	17.92631813086884	0.0
35	0.0	0.0	0.0	18.34283396501267	0.0
36	0.0	0.0	0.0	18.768072643850612	0.0
37	0.0	0.0	0.0	19.161472939555047	0.0
38	0.0	0.0	0.0	19.50995058508481	0.0
39	0.0	0.0	0.0	19.908148445371005	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.021461E-4	45.000004	1
ACTATAT	20	7.021461E-4	45.000004	40
CACGGGC	20	7.021461E-4	45.000004	4
CCTAGGA	20	7.021461E-4	45.000004	4
GACCGAT	40	6.7739165E-9	45.000004	9
TCGATGG	20	7.021461E-4	45.000004	2
ATATCGG	35	1.2062446E-7	45.000004	9
CTAACGG	20	7.021461E-4	45.000004	2
CGGATAC	20	7.021461E-4	45.000004	14
TCACTAA	20	7.021461E-4	45.000004	11
GGCATGT	20	7.021461E-4	45.000004	8
GCGGATA	20	7.021461E-4	45.000004	13
GTAAGTT	20	7.021461E-4	45.000004	10
TACCGAG	20	7.021461E-4	45.000004	1
AAGTCCG	20	7.021461E-4	45.000004	30
AACACGT	20	7.021461E-4	45.000004	41
GCGAGAC	25	3.8810074E-5	45.0	21
ACACGAC	25	3.8810074E-5	45.0	26
ATGGACG	25	3.8810074E-5	45.0	21
TGGGTAC	50	2.1827873E-11	45.0	6
>>END_MODULE