##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936654.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	216212
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.073645311083567	31.0	31.0	33.0	28.0	34.0
2	31.13476125284443	31.0	31.0	34.0	28.0	34.0
3	30.885001757534273	31.0	31.0	34.0	27.0	34.0
4	34.81657817327438	35.0	35.0	37.0	32.0	37.0
5	35.08524966236842	35.0	35.0	37.0	33.0	37.0
6	35.07438070042366	35.0	35.0	37.0	32.0	37.0
7	31.116339518620613	35.0	35.0	37.0	0.0	37.0
8	33.028462805024695	35.0	35.0	37.0	17.0	37.0
9	36.151749209109575	39.0	35.0	39.0	32.0	39.0
10	36.07210978114073	37.0	35.0	39.0	32.0	39.0
11	36.307022736943374	37.0	35.0	39.0	32.0	39.0
12	36.3036094203837	38.0	35.0	39.0	32.0	39.0
13	36.11517399589292	38.0	35.0	39.0	31.0	39.0
14	37.17588755480732	39.0	36.0	40.0	32.0	41.0
15	37.32118476310288	39.0	36.0	41.0	32.0	41.0
16	37.40211921632472	39.0	36.0	40.0	32.0	41.0
17	37.217346863263835	39.0	35.0	40.0	32.0	41.0
18	37.11073390931123	39.0	36.0	40.0	32.0	41.0
19	36.86959095702366	38.0	35.0	40.0	31.0	41.0
20	36.76890736869369	38.0	35.0	40.0	31.0	41.0
21	36.87111261169593	38.0	35.0	40.0	32.0	41.0
22	36.96788337372579	39.0	35.0	40.0	32.0	41.0
23	37.00808928274101	39.0	35.0	40.0	32.0	41.0
24	36.86640426988326	38.0	35.0	40.0	32.0	41.0
25	36.64942278874438	38.0	35.0	40.0	31.0	41.0
26	36.54767542967088	38.0	35.0	40.0	31.0	41.0
27	36.5713050154478	38.0	35.0	40.0	31.0	41.0
28	36.57704937746286	38.0	35.0	40.0	31.0	41.0
29	36.46467818622463	38.0	35.0	40.0	31.0	41.0
30	36.28200562410967	38.0	35.0	40.0	30.0	41.0
31	36.23589347492276	38.0	35.0	40.0	30.0	41.0
32	36.010942963387784	38.0	35.0	40.0	30.0	41.0
33	35.7815616154515	38.0	35.0	40.0	29.0	41.0
34	35.64285516067563	38.0	35.0	40.0	28.0	41.0
35	35.58355225426896	38.0	35.0	40.0	27.0	41.0
36	35.44865224871885	38.0	35.0	40.0	27.0	41.0
37	35.35281575490722	38.0	35.0	40.0	26.0	41.0
38	35.17226148409894	38.0	34.0	40.0	25.0	41.0
39	35.031362736573364	38.0	34.0	40.0	25.0	41.0
40	34.78260688583428	38.0	34.0	40.0	23.0	41.0
41	34.64435368989695	38.0	34.0	40.0	23.0	41.0
42	34.610881912197286	38.0	34.0	40.0	23.0	41.0
43	34.487794386990544	38.0	34.0	40.0	23.0	41.0
44	34.249213734667826	38.0	33.0	40.0	21.0	41.0
45	34.15667955525133	37.0	33.0	40.0	22.0	41.0
46	33.8954960871737	37.0	33.0	40.0	20.0	41.0
47	33.69505855364179	37.0	33.0	40.0	20.0	41.0
48	33.65229496975191	37.0	33.0	40.0	19.0	41.0
49	33.59592899561542	37.0	33.0	40.0	19.0	41.0
50	33.452097940909844	36.0	33.0	40.0	18.0	41.0
51	31.577442510128947	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	11.0
10	15.0
11	14.0
12	12.0
13	9.0
14	8.0
15	16.0
16	30.0
17	53.0
18	113.0
19	271.0
20	418.0
21	698.0
22	985.0
23	1428.0
24	2007.0
25	2872.0
26	3619.0
27	4243.0
28	4510.0
29	5086.0
30	5872.0
31	7170.0
32	8904.0
33	12372.0
34	18927.0
35	20372.0
36	20091.0
37	28776.0
38	37694.0
39	29608.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08832072225409	19.39207814552384	22.23095850369082	16.288642628531257
2	31.445988196769836	28.27271381791945	24.64201801935138	15.639279965959336
3	28.739847927034578	26.68492035594694	29.3087340203134	15.266497696705086
4	25.95138105193051	23.309992044844876	34.52213568164579	16.21649122157882
5	26.286237581632843	26.849573566684548	27.646476606293824	19.217712245388785
6	23.88951584555899	36.09235380089912	27.389784100789967	12.628346252751928
7	63.36003552069265	16.60499879747655	16.544410115997263	3.4905555658335334
8	73.06347473775739	9.092927312082585	12.776811647827133	5.066786302332896
9	68.75104064529258	5.484431946422955	14.363680091761792	11.400847316522672
10	41.1498899228535	22.477938319797236	21.077461010489703	15.294710746859563
11	30.54363310084547	24.581429337872088	27.421697223095848	17.453240338186596
12	26.71729598727175	21.390117107283594	31.575028213050153	20.317558692394503
13	21.547370173718388	24.416313618115552	36.15618004551089	17.880136162655173
14	18.101677982720663	29.107542597080645	31.331285960076222	21.459493460122474
15	16.5254472462213	23.1101881486689	41.759014300778865	18.605350304330933
16	18.48463545039128	23.11897582002849	34.5794867999926	23.816901929587626
17	18.619688083917637	23.36641814515383	33.54994172386361	24.463952047064918
18	18.831054705566757	23.656873809039276	34.827391634136866	22.6846798512571
19	20.368897193495272	25.488409523985716	31.126856973710986	23.015836308808023
20	22.703180212014136	24.697056592603555	33.35938800806616	19.240375187316154
21	20.386472536214455	28.586757441770118	31.727193680276766	19.299576341738664
22	20.15105544558119	22.862283314524635	31.918209905093146	25.06845133480103
23	18.674726657169817	27.295432260929086	31.550052726028156	22.47978835587294
24	21.417867648419143	23.316467171109835	31.290122657391816	23.9755425230792
25	18.391208628568258	29.113092705307754	30.682848315542156	21.812850350581837
26	17.72704567739071	26.32971342941187	32.37701885186761	23.566222041329805
27	19.50354281908497	26.73533383900986	31.158770096016873	22.602353245888295
28	16.2053910051246	25.263630140787747	36.66817752946183	21.86280132462583
29	20.075203966477346	25.25622999648493	32.80391467633619	21.864651360701533
30	19.846724511127967	26.998963979797608	34.06008917173885	19.09422233733558
31	24.3349120307846	26.184023088450225	28.26346363754093	21.217601243224244
32	25.839453869350454	27.095165855734187	27.62797624553679	19.437404029378573
33	21.788799881597694	26.726546167650266	27.22143081790095	24.263223132851092
34	23.11666327493386	26.87778661683903	28.52894381440438	21.47660629382273
35	19.36386509536936	24.11753279188944	30.651397702255196	25.867204410486007
36	21.144524818233958	29.06175420420698	28.01602131241559	21.777699665143473
37	22.294784748302593	25.127189980204612	32.53473442732133	20.04329084417146
38	19.788910883762235	30.058923649011156	26.195585813923373	23.95657965330324
39	25.176678445229683	24.576341738663903	27.921206963535788	22.325772852570623
40	21.234714076924497	25.54252307920005	29.26340813645866	23.959354707416793
41	19.864762362866077	24.892235398590273	27.783379275895882	27.459622962647774
42	19.7704105230052	23.81320185743622	30.349379312896602	26.067008306661982
43	21.461343496198175	23.396018722365085	28.492405601909237	26.6502321795275
44	21.104286533587405	25.55639834976782	29.965959336207053	23.37335578043772
45	19.863374835809296	24.968086877694116	28.385103509518434	26.783434776978147
46	23.354855419680685	26.58548091687788	27.426322313285105	22.63334135015633
47	19.18811166817753	23.587034947181472	34.2344550718739	22.9903983127671
48	20.42393576674745	24.511127966995357	30.0686363384086	24.996299927848593
49	19.56413150056426	22.02051690007955	33.745583038869256	24.66976856048693
50	20.99837196825338	22.40162433167447	31.74985662220413	24.85014707786802
51	19.74219747285072	23.964904815643905	29.010878212125135	27.28201949938024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	76.0
1	201.0
2	326.0
3	2242.0
4	4158.0
5	2801.0
6	1444.0
7	1356.5
8	1269.0
9	1306.0
10	1343.0
11	1259.0
12	1175.0
13	1117.0
14	1059.0
15	979.5
16	900.0
17	826.0
18	752.0
19	743.0
20	734.0
21	778.5
22	823.0
23	869.0
24	915.0
25	1070.0
26	1578.0
27	1931.0
28	2097.5
29	2264.0
30	2764.0
31	3264.0
32	3803.0
33	4342.0
34	4805.5
35	5269.0
36	5736.5
37	6204.0
38	6789.5
39	7375.0
40	8807.0
41	10239.0
42	11376.0
43	12513.0
44	14591.0
45	16669.0
46	22963.5
47	29258.0
48	24616.5
49	19975.0
50	19286.0
51	18597.0
52	15875.5
53	13154.0
54	11590.5
55	10027.0
56	9505.5
57	8984.0
58	8186.5
59	7389.0
60	7130.5
61	6872.0
62	5825.5
63	4779.0
64	4343.0
65	3907.0
66	3374.0
67	2841.0
68	2183.0
69	1525.0
70	1257.0
71	989.0
72	813.0
73	637.0
74	594.0
75	382.0
76	213.0
77	177.5
78	142.0
79	90.5
80	39.0
81	37.5
82	36.0
83	25.5
84	15.0
85	10.5
86	6.0
87	5.0
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	216212.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.832886241281706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.01452081316553	27.59652563224983
2	8.356243949661181	5.988566777052153
3	3.2333010648596323	3.475755277227906
4	1.9270732494353016	2.76210386102529
5	1.2675056469828976	2.270919282926017
6	0.8493062278154243	1.8259856067193312
7	0.6440787350758309	1.6155440031080608
8	0.5627621813488223	1.6132314580134315
9	0.4543401097128106	1.4652285719571532
>10	5.569538560826072	41.48104638040442
>50	0.08389803162310422	1.8259856067193312
>100	0.02968699580509842	2.2237433629955783
>500	0.0025814778960955146	0.7039387268051727
>1k	0.005162955792191029	5.151425452796329
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC	3250	1.5031543115090744	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG	3202	1.4809538786006327	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2407	1.1132592085545667	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC	2279	1.0540580541320554	No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	965	0.44632120326346364	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	557	0.2576175235417091	No Hit
GAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT	475	0.21969178398978778	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTC	473	0.21876676595193606	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTT	416	0.19240375187316153	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTA	387	0.17899099032431132	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	355	0.16419070171868352	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11146467356113444	0.0
2	0.0	0.0	0.0	1.038332747488576	0.0
3	0.0	0.0	0.0	1.264499657743326	0.0
4	0.0	0.0	0.0	1.7996225926405565	0.0
5	0.0	0.0	0.0	3.9618522561189944	0.0
6	0.0	0.0	0.0	4.730542245573789	0.0
7	0.0	0.0	0.0	5.5020072891421385	0.0
8	0.0	0.0	0.0	6.635616894529443	0.0
9	0.0	0.0	0.0	6.982498658723845	0.0
10	0.0	0.0	0.0	9.523060699683644	0.0
11	0.0	0.0	0.0	10.471204188481675	0.0
12	0.0	0.0	0.0	13.103343015188797	0.0
13	0.0	0.0	0.0	13.630603296764287	0.0
14	0.0	0.0	0.0	13.892383401476328	0.0
15	0.0	0.0	0.0	14.347029767080459	0.0
16	0.0	0.0	0.0	14.937653784248793	0.0
17	0.0	0.0	0.0	15.535215436701016	0.0
18	0.0	0.0	0.0	16.293267718720514	0.0
19	0.0	0.0	0.0	16.748839102362496	0.0
20	0.0	0.0	0.0	17.15584703901726	0.0
21	0.0	0.0	0.0	17.61835605794313	0.0
22	0.0	0.0	0.0	18.075314968641887	0.0
23	0.0	0.0	0.0	18.520248644848575	0.0
24	0.0	0.0	0.0	18.925406545427634	0.0
25	0.0	0.0	0.0	19.251012894751447	0.0
26	0.0	0.0	0.0	19.58679444249163	0.0
27	4.6250901892586906E-4	0.0	0.0	19.902688102417997	0.0
28	4.6250901892586906E-4	0.0	0.0	20.194068784341294	0.0
29	4.6250901892586906E-4	0.0	0.0	20.55297578302777	0.0
30	4.6250901892586906E-4	0.0	0.0	20.920670453073836	0.0
31	4.6250901892586906E-4	0.0	0.0	21.27078978040072	0.0
32	4.6250901892586906E-4	0.0	0.0	21.58853347640279	0.0
33	4.6250901892586906E-4	0.0	0.0	21.922927497086192	0.0
34	4.6250901892586906E-4	0.0	0.0	22.293397221245815	0.0
35	4.6250901892586906E-4	0.0	0.0	22.691617486540988	0.0
36	4.6250901892586906E-4	0.0	0.0	23.01028620058091	0.0
37	4.6250901892586906E-4	0.0	0.0	23.35624294673746	0.0
38	4.6250901892586906E-4	0.0	0.0	23.70682478308327	0.0
39	4.6250901892586906E-4	0.0	0.0	24.04260633082345	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGTC	20	7.0205715E-4	45.0	35
CGAGTTA	20	7.0205715E-4	45.0	32
GGCACCC	20	7.0205715E-4	45.0	8
GTTTACA	20	7.0205715E-4	45.0	9
GGCGTAT	20	7.0205715E-4	45.0	8
ACCTGAC	25	3.8802697E-5	45.0	42
GGTTTAC	20	7.0205715E-4	45.0	8
CGATTAT	25	3.8802697E-5	45.0	10
TAACGCC	20	7.0205715E-4	45.0	12
TGACGAA	20	7.0205715E-4	45.0	38
CCAGATA	25	3.8802697E-5	45.0	21
TCGGCGG	20	7.0205715E-4	45.0	3
GCCGATG	20	7.0205715E-4	45.0	10
AACATAG	20	7.0205715E-4	45.0	1
TGATTGG	20	7.0205715E-4	45.0	2
CGTAGCC	25	3.8802697E-5	45.0	16
AACGCCG	20	7.0205715E-4	45.0	13
TCGTCCC	20	7.0205715E-4	45.0	38
CGAATAT	55	1.8189894E-12	45.0	14
ATGGTAA	20	7.0205715E-4	45.0	12
>>END_MODULE