##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936652.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341931
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09586437029693	31.0	31.0	33.0	28.0	34.0
2	31.156420447400208	31.0	31.0	34.0	28.0	34.0
3	30.82910587223738	31.0	30.0	34.0	27.0	34.0
4	34.75343563467483	35.0	35.0	37.0	32.0	37.0
5	35.08548800781444	35.0	35.0	37.0	33.0	37.0
6	35.10545109978329	35.0	35.0	37.0	32.0	37.0
7	31.03328449307024	35.0	35.0	37.0	0.0	37.0
8	33.07041771585495	36.0	35.0	37.0	17.0	37.0
9	36.29346271616203	39.0	35.0	39.0	32.0	39.0
10	36.1104696561587	37.0	35.0	39.0	32.0	39.0
11	36.335517984622626	37.0	35.0	39.0	32.0	39.0
12	36.422362991363755	38.0	35.0	39.0	32.0	39.0
13	36.19674437240262	38.0	35.0	39.0	32.0	39.0
14	37.30268972395015	39.0	36.0	40.0	32.0	41.0
15	37.468214347339085	39.0	36.0	41.0	32.0	41.0
16	37.55264658659203	39.0	36.0	40.0	33.0	41.0
17	37.390552479886296	39.0	36.0	40.0	32.0	41.0
18	37.25016158230754	39.0	36.0	40.0	32.0	41.0
19	36.99084903094484	38.0	36.0	40.0	32.0	41.0
20	36.89067969853567	38.0	35.0	40.0	32.0	41.0
21	36.959447958798705	39.0	35.0	40.0	32.0	41.0
22	37.03009671541919	39.0	35.0	40.0	32.0	41.0
23	37.115295191135054	39.0	35.0	40.0	32.0	41.0
24	36.990427893346904	39.0	35.0	40.0	32.0	41.0
25	36.77565356753263	38.0	35.0	40.0	32.0	41.0
26	36.73271507994303	38.0	35.0	40.0	32.0	41.0
27	36.7828158312642	38.0	35.0	40.0	32.0	41.0
28	36.80008539734625	38.0	35.0	40.0	32.0	41.0
29	36.79171236302061	39.0	35.0	40.0	32.0	41.0
30	36.62049360835958	38.0	35.0	40.0	31.0	41.0
31	36.534134079682744	38.0	35.0	40.0	31.0	41.0
32	36.29308544706388	38.0	35.0	40.0	30.0	41.0
33	36.02837707022762	38.0	35.0	40.0	30.0	41.0
34	35.89931886842667	38.0	35.0	40.0	29.0	41.0
35	35.757319459189134	38.0	35.0	40.0	28.0	41.0
36	35.66146386259216	38.0	35.0	40.0	27.0	41.0
37	35.55664739377243	38.0	35.0	40.0	27.0	41.0
38	35.475353214537435	38.0	35.0	40.0	27.0	41.0
39	35.44225004459964	38.0	35.0	40.0	26.0	41.0
40	35.37111873448152	38.0	35.0	40.0	25.0	41.0
41	35.22231385864399	38.0	35.0	40.0	25.0	41.0
42	35.26246523421392	38.0	35.0	40.0	25.0	41.0
43	35.17752996949677	38.0	35.0	40.0	25.0	41.0
44	35.00345683778306	38.0	34.0	40.0	24.0	41.0
45	34.930746261672674	38.0	34.0	40.0	24.0	41.0
46	34.82444996212686	38.0	34.0	40.0	23.0	41.0
47	34.776162442130136	38.0	34.0	40.0	23.0	41.0
48	34.74516203561537	38.0	34.0	40.0	23.0	41.0
49	34.68240960895619	38.0	34.0	40.0	23.0	41.0
50	34.47990676481513	38.0	34.0	40.0	23.0	41.0
51	32.79546750660221	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	11.0
10	20.0
11	14.0
12	7.0
13	4.0
14	8.0
15	12.0
16	37.0
17	72.0
18	155.0
19	344.0
20	524.0
21	874.0
22	1284.0
23	2006.0
24	2990.0
25	4427.0
26	5662.0
27	6065.0
28	6054.0
29	6375.0
30	7563.0
31	9499.0
32	12706.0
33	17777.0
34	27958.0
35	32626.0
36	32877.0
37	47700.0
38	64475.0
39	51794.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.169689206301854	20.049951598421902	25.43378634870778	16.34657284656846
2	32.731457516282525	27.165129806890864	24.36193267062653	15.741480006200081
3	27.254036633121885	26.440422190441932	32.15648771243321	14.149053464002973
4	25.086347830410226	22.716863928687367	36.64306541378231	15.553722827120092
5	26.141239021907932	26.31466582439147	30.338869538006207	17.205225615694395
6	23.110510600091832	34.615463353717566	29.318488233006075	12.955537813184531
7	63.468068119006475	16.15793829749277	16.994949273391416	3.3790443101093497
8	74.10325475022739	7.298548537570445	13.920644808455506	4.677551903746662
9	70.11385337977545	4.792779829848713	15.369475128022906	9.72389166235293
10	37.80002398144655	27.1680543735432	22.21413092115076	12.817790723859494
11	27.011005144312744	24.09258008194636	31.97048527334462	16.92592950039628
12	25.17349991664985	20.156990737897413	35.85343241765152	18.816076927801223
13	19.460943874641377	25.59609979791244	39.419648993510386	15.523307333935794
14	16.44162126276939	29.851636733726977	34.24901515217982	19.457726851323805
15	13.333976737996847	26.18715471834961	44.682699141054776	15.79616940259877
16	16.222278763844166	25.42735230207264	37.041976305160986	21.30839262892221
17	17.116026332798135	24.432999640278304	35.13749850115959	23.31347552576397
18	18.355457680058258	24.201666418078503	37.66812602542618	19.77474987643706
19	18.30837215695564	26.91537181478135	34.07412606637011	20.702129961892897
20	20.377210606818334	26.544536763265103	36.57667775077428	16.501574879142282
21	18.944172947173552	29.218760510161406	34.30545928856992	17.531607254095125
22	16.791106977723576	23.882011282978144	34.89446701235045	24.432414726947833
23	15.805235559221012	27.27626333967964	34.62072757369177	22.29777352740758
24	19.198317789261576	23.43542995516639	35.6419862486876	21.72426600688443
25	14.942488396781805	29.05966408427431	33.94749233032395	22.05035518861993
26	16.172561130754453	26.9384758913348	35.304783713673224	21.58417926423752
27	17.10579034951496	29.409734712558965	33.226294193857825	20.258180744068248
28	13.558875913561508	26.274891717919697	40.509927441501354	19.65630492701744
29	18.452260836250588	22.20798933118085	37.59325711912637	21.746492713442187
30	16.669445004986386	28.212417125092486	36.15378541284645	18.964352457074675
31	20.524023852765616	24.851505128227625	33.789565731097795	20.834905287908963
32	21.772813813313213	27.1028365371961	31.553442068721466	19.57090758076922
33	18.175304374274344	26.280740851224373	30.74860132599852	24.79535344850277
34	17.88197033904501	23.58429039777031	36.91183308913202	21.62190617405266
35	16.7826257344318	26.046190605706997	32.002363049855084	25.168820610006108
36	18.64411240864385	26.789030535400414	35.02870462169268	19.538152434263054
37	18.768406491368143	27.504964451892338	35.5358244792078	18.190804577531726
38	17.635721826918296	26.29068437784231	30.73076146941927	25.34283232582012
39	21.145201809721843	24.738616855447443	33.65971497173406	20.45646636309665
40	18.305447590303306	23.75830211358432	32.83674191576663	25.099508380345743
41	16.220231567187533	26.289222094516145	32.836449459101395	24.654096879194924
42	18.799406897882907	24.45347160684466	33.15815179085839	23.588969704414048
43	20.687214671965982	26.23599498144362	30.59242946676376	22.48436087982663
44	19.09303338977747	24.49149097332503	32.820656799178785	23.59481883771872
45	18.340834846796575	24.876656401437717	30.367237834533867	26.415270917231837
46	22.029298308723106	26.530791299999123	31.127625164141303	20.31228522713647
47	16.358271113177807	24.13615612506617	37.57161532589908	21.93395743585694
48	19.033664686735044	24.157505461628222	32.650447020012805	24.15838283162392
49	18.114473387905747	22.065562935212075	36.62376327387689	23.196200403005285
50	18.9906735569457	22.62210796915167	34.181750119176094	24.205468354726538
51	18.24110712395191	23.123086236696878	30.161348342209394	28.47445829714182
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	140.0
1	261.0
2	382.0
3	4326.5
4	8271.0
5	5536.0
6	2801.0
7	2610.5
8	2420.0
9	2385.5
10	2351.0
11	2336.0
12	2321.0
13	2291.0
14	2261.0
15	2156.5
16	2052.0
17	1842.0
18	1632.0
19	1647.0
20	1662.0
21	1873.0
22	2084.0
23	2101.0
24	2118.0
25	2407.0
26	3073.0
27	3450.0
28	4019.0
29	4588.0
30	5590.5
31	6593.0
32	7876.5
33	9160.0
34	10221.0
35	11282.0
36	11948.5
37	12615.0
38	13800.5
39	14986.0
40	17058.5
41	19131.0
42	23215.0
43	27299.0
44	40436.0
45	53573.0
46	44620.0
47	35667.0
48	33752.0
49	31837.0
50	26947.5
51	22058.0
52	18669.5
53	15281.0
54	13269.0
55	11257.0
56	9908.0
57	8559.0
58	7659.0
59	6759.0
60	6037.5
61	5316.0
62	4475.5
63	3635.0
64	2884.0
65	2133.0
66	1692.5
67	1252.0
68	1019.0
69	786.0
70	661.5
71	537.0
72	473.5
73	410.0
74	361.5
75	241.5
76	170.0
77	104.0
78	38.0
79	25.0
80	12.0
81	17.0
82	22.0
83	19.5
84	17.0
85	9.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	341931.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.4857068710422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19008886497504	26.681734530701178
2	10.194523356184742	7.235197350151301
3	3.86012881054738	4.109381983666474
4	2.115745215304178	3.0031485809637646
5	1.309708810790631	2.32379714730688
6	0.9063179454660829	1.9296799766864776
7	0.6908126015627175	1.715978143731366
8	0.4902430806820519	1.3917297805311986
9	0.4299921785534247	1.3732718764488898
>10	4.6085851773002595	36.20641128517323
>50	0.1535094587280637	3.346338550681528
>100	0.03796470484672543	2.3359352599541285
>500	0.007427877035228889	1.753122927041802
>1k	0.0033012786823239508	3.5428546689650315
>5k	0.0016506393411619754	3.0514179379967485
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGC	5254	1.5365673191374867	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG	5165	1.5105386759316937	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4889	1.4298206363272121	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC	4019	1.175383337573955	No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	2120	0.6200081302952934	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT	1069	0.3126361751347494	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTC	801	0.23425778885213683	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	780	0.22811619888223064	No Hit
GAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT	761	0.22255952224279169	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTATGCTCATCGTA	708	0.20705931898540933	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGC	630	0.18424769909718627	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG	622	0.18190804577531725	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	620	0.18132313244485	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT	562	0.1643606458612995	No Hit
TCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	502	0.14681324594728176	No Hit
GAATCTATCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC	482	0.14096411264260916	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	355	0.1038221161579383	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19799316236316683	0.0
2	0.0	0.0	0.0	1.428358353001044	0.0
3	0.0	0.0	0.0	1.7836932012599034	0.0
4	0.0	0.0	0.0	2.4560510746320166	0.0
5	0.0	0.0	0.0	4.77493997326946	0.0
6	0.0	0.0	0.0	5.5397141528554	0.0
7	0.0	0.0	0.0	6.464754584989369	0.0
8	0.0	0.0	0.0	7.914462274552468	0.0
9	0.0	0.0	0.0	8.312203339270203	0.0
10	0.0	0.0	0.0	10.934077343089688	0.0
11	0.0	0.0	0.0	12.483805212162688	0.0
12	0.0	0.0	0.0	15.377371457984212	0.0
13	0.0	0.0	0.0	15.941812821885117	0.0
14	0.0	0.0	0.0	16.17811780739389	0.0
15	0.0	0.0	0.0	16.72705896803741	0.0
16	0.0	0.0	0.0	17.471361181057	0.0
17	0.0	0.0	0.0	18.434128523006102	0.0
18	0.0	0.0	0.0	19.450122978027732	0.0
19	0.0	0.0	0.0	20.133594204678722	0.0
20	0.0	0.0	0.0	20.777876238188405	0.0
21	0.0	0.0	0.0	21.501998941306873	0.0
22	0.0	0.0	0.0	22.292801764098606	0.0
23	0.0	0.0	0.0	23.07424597360286	0.0
24	0.0	0.0	0.0	23.680801097297408	0.0
25	0.0	0.0	0.0	24.18850586814299	0.0
26	0.0	0.0	0.0	24.669597082452306	0.0
27	0.0	0.0	0.0	25.14776373010929	0.0
28	0.0	0.0	0.0	25.62476055110534	0.0
29	0.0	0.0	0.0	26.102342285431856	0.0
30	0.0	0.0	0.0	26.715331455761543	0.0
31	0.0	0.0	0.0	27.26164050641796	0.0
32	0.0	0.0	0.0	27.712901140873452	0.0
33	0.0	0.0	0.0	28.17147319195978	0.0
34	0.0	0.0	0.0	28.66630986953508	0.0
35	0.0	0.0	0.0	29.20764715688253	0.0
36	0.0	0.0	0.0	29.708625424427737	0.0
37	0.0	0.0	0.0	30.159008688887525	0.0
38	0.0	0.0	0.0	30.6310337465746	0.0
39	0.0	0.0	0.0	31.102766347596447	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGCG	20	7.0263265E-4	45.000004	27
CGAGTGT	20	7.0263265E-4	45.000004	19
TCCGAGT	20	7.0263265E-4	45.000004	17
GACACGT	20	7.0263265E-4	45.000004	24
GCGCGAG	20	7.0263265E-4	45.000004	1
GCGCGAA	20	7.0263265E-4	45.000004	1
ACACCGC	25	3.8850372E-5	45.000004	15
CGTTGCG	20	7.0263265E-4	45.000004	2
CGACCGG	20	7.0263265E-4	45.000004	2
TGAACGC	40	6.7902874E-9	45.000004	29
CGCGAAG	20	7.0263265E-4	45.000004	2
ATCGTGG	20	7.0263265E-4	45.000004	2
AACGGCG	20	7.0263265E-4	45.000004	7
AATGCGG	20	7.0263265E-4	45.000004	2
CTAACGG	20	7.0263265E-4	45.000004	2
GTATGCG	25	3.8850372E-5	45.000004	1
AACGCTT	40	6.7902874E-9	45.000004	31
TCGCTAT	40	6.7902874E-9	45.000004	23
CCGAGTG	20	7.0263265E-4	45.000004	18
ACGCTTG	40	6.7902874E-9	45.000004	32
>>END_MODULE