##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936651.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 827524 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.147160686578275 31.0 31.0 33.0 28.0 34.0 2 31.17417017512483 31.0 31.0 34.0 28.0 34.0 3 30.98737680115622 31.0 31.0 34.0 28.0 34.0 4 34.95255243352459 35.0 35.0 37.0 32.0 37.0 5 35.17491214756309 35.0 35.0 37.0 33.0 37.0 6 35.13854462227077 36.0 35.0 37.0 32.0 37.0 7 31.064674861393748 35.0 35.0 37.0 0.0 37.0 8 33.03412348161503 35.0 35.0 37.0 17.0 37.0 9 36.2307123418777 39.0 35.0 39.0 32.0 39.0 10 36.085726818799216 37.0 35.0 39.0 32.0 39.0 11 36.34892522754627 37.0 35.0 39.0 32.0 39.0 12 36.42125908130761 38.0 35.0 39.0 32.0 39.0 13 36.34012789961379 38.0 35.0 39.0 32.0 39.0 14 37.38925275883237 39.0 36.0 40.0 32.0 41.0 15 37.533789956545064 39.0 36.0 41.0 32.0 41.0 16 37.511020828398934 39.0 36.0 41.0 32.0 41.0 17 37.39613835973337 39.0 36.0 40.0 32.0 41.0 18 37.32933425495816 39.0 36.0 40.0 32.0 41.0 19 37.14635829293169 39.0 36.0 40.0 32.0 41.0 20 37.106986625161326 39.0 35.0 40.0 32.0 41.0 21 37.155201540982496 39.0 35.0 40.0 32.0 41.0 22 37.269184942068144 39.0 35.0 40.0 32.0 41.0 23 37.3275735809475 39.0 35.0 40.0 32.0 41.0 24 37.24706594612362 39.0 35.0 41.0 32.0 41.0 25 36.95251255552709 39.0 35.0 40.0 31.0 41.0 26 36.912675644452605 39.0 35.0 40.0 31.0 41.0 27 36.9462082066502 39.0 35.0 40.0 31.0 41.0 28 36.93923318236087 39.0 35.0 40.0 31.0 41.0 29 36.922678979703306 39.0 35.0 40.0 31.0 41.0 30 36.710544950962145 39.0 35.0 40.0 31.0 41.0 31 36.721200835262785 39.0 35.0 40.0 31.0 41.0 32 36.58683252691161 39.0 35.0 40.0 31.0 41.0 33 36.45381161150613 39.0 35.0 40.0 30.0 41.0 34 36.38248920877219 39.0 35.0 40.0 30.0 41.0 35 36.33918895403638 39.0 35.0 40.0 30.0 41.0 36 36.26915593988815 39.0 35.0 40.0 30.0 41.0 37 36.195155669201135 39.0 35.0 40.0 30.0 41.0 38 36.07000642881657 38.0 35.0 40.0 30.0 41.0 39 36.03179001454943 38.0 35.0 40.0 29.0 41.0 40 35.89759209400573 38.0 35.0 40.0 29.0 41.0 41 35.75553699953113 38.0 35.0 40.0 28.0 41.0 42 35.74898371527593 38.0 35.0 40.0 29.0 41.0 43 35.70475176550771 38.0 35.0 40.0 28.0 41.0 44 35.53097916193367 38.0 34.0 40.0 28.0 41.0 45 35.453785026174465 38.0 34.0 40.0 28.0 41.0 46 35.38075028639653 38.0 34.0 40.0 27.0 41.0 47 35.322822057124625 38.0 34.0 40.0 27.0 41.0 48 35.254705603704544 38.0 34.0 40.0 27.0 41.0 49 35.12738482509268 37.0 34.0 40.0 27.0 41.0 50 34.966520608465736 37.0 34.0 40.0 26.0 41.0 51 33.29047133376192 35.0 31.0 39.0 22.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 30.0 9 70.0 10 76.0 11 53.0 12 47.0 13 30.0 14 34.0 15 64.0 16 122.0 17 221.0 18 433.0 19 823.0 20 1341.0 21 1942.0 22 2828.0 23 3968.0 24 5435.0 25 7224.0 26 9086.0 27 10837.0 28 12561.0 29 14887.0 30 18238.0 31 23641.0 32 31195.0 33 43083.0 34 65027.0 35 71321.0 36 84385.0 37 124380.0 38 163156.0 39 130974.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.54671526143048 20.449799643273188 22.500495453908282 11.502989641388044 2 32.56775634301845 26.43101589802834 24.25754419207177 16.743683566881444 3 33.05753065772111 25.436724493791118 25.747893716677705 15.757851131810074 4 28.663337860895876 24.868765135512685 27.375399384187045 19.09249761940439 5 27.348209840439676 29.85677756778051 23.36306862399157 19.431943967788246 6 25.517568070533304 36.145537772922594 24.402313407224444 13.934580749319657 7 67.50366152522464 18.15753984174477 10.707000642881656 3.6317979901489257 8 77.68511849807376 8.279276492283003 9.526732759412415 4.508872250230809 9 70.83685790381911 8.743794741904766 11.592775556962698 8.82657179731343 10 41.2614014820114 25.246639372392828 18.79087494743355 14.701084198162228 11 32.47494936702742 25.047853596995374 24.176096403246312 18.301100632730893 12 28.50600103441109 23.251893600668982 28.09972882961703 20.1423765353029 13 25.030452288997058 24.596507170789003 30.492166994552424 19.880873545661515 14 20.944770181892007 27.7985895273128 29.569414300974955 21.687225989820234 15 19.92800208815696 25.40893073796047 34.19622874986103 20.46683842402154 16 22.556566335236198 25.99417056182056 29.97290713018595 21.476355972757286 17 23.258660776001662 25.725779554429838 28.60352086465166 22.412038804916836 18 24.14528158699929 25.890971137997205 28.5765730057376 21.387174269265905 19 25.38053277004655 26.755961156413587 26.344734412536674 21.518771661003186 20 25.883478908164598 26.210599330049643 28.844843170711666 19.061078591074097 21 24.7696743538556 27.863361062639875 28.183714309192244 19.183250274312286 22 23.738646854955263 24.207032061909985 28.514580845993592 23.539740237141157 23 23.209477912423086 27.07015143971655 29.122176516934857 20.598194130925506 24 23.384941101406124 24.897525630676572 29.018010353778255 22.699522914139045 25 22.851059304624396 28.134168918363695 26.856381204653886 22.158390572358023 26 21.868247929969403 27.18374331137224 27.554487845669733 23.393520912988627 27 22.672091685558364 27.554487845669733 27.698532006322473 22.074888462449426 28 20.05234893489494 27.427603308181997 29.88396711152788 22.63608064539518 29 22.85649721337387 28.14299041477951 26.964414325143437 22.036098046703177 30 22.22159115626858 28.205465944190138 28.692581725726384 20.880361173814897 31 23.95592152010093 26.642973496841176 26.77191235541205 22.629192627645843 32 25.455092541122674 28.423828191085697 26.16612932072061 19.95494994707102 33 23.64161039438131 27.832787931226164 25.66330402501921 22.86229764937331 34 22.384728418752807 28.01344734412537 27.809223659978443 21.792600577143382 35 22.3299868040081 28.85608151546058 26.227758953214654 22.58617272731667 36 23.347479952243077 30.19537801924778 26.301714512207504 20.155427516301643 37 23.34349215249346 29.3231374558321 27.039578308302843 20.2937920833716 38 22.466780419661543 29.21848792300888 26.615904795510463 21.69882686181911 39 24.702969339862047 26.241414146296666 25.815927997254462 23.239688516586828 40 23.637501752215044 27.258907294531635 27.710132878321353 21.393458074931964 41 21.28349147577593 27.2300259569511 27.233409544617437 24.253073022655535 42 22.46279261991193 27.423857193265693 27.815869992894466 22.29748019392791 43 24.05851673184101 26.136522928640137 26.960909895060446 22.844050444458407 44 22.89661689570333 25.457388547039116 27.784088437314203 23.86190611994335 45 22.0124129330388 26.083110580478632 26.949792392728188 24.95468409375438 46 23.24234704975324 27.383374983686277 25.967343545323157 23.40693442123733 47 21.04688202396547 26.536148800518173 29.87997931177827 22.536989863738093 48 22.120446053528354 27.380837292936523 27.33201695660791 23.166699696927218 49 21.992836461540694 25.689768514266653 29.584277918223517 22.733117105969132 50 20.973651519472547 26.293859755124927 28.720858851223653 24.011629874178876 51 21.147181229789105 25.49629980520202 26.996437565557013 26.360081399451857 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 340.0 1 969.0 2 1598.0 3 4322.0 4 7046.0 5 4748.0 6 2450.0 7 2257.5 8 2065.0 9 2081.5 10 2098.0 11 2052.5 12 2007.0 13 1867.5 14 1728.0 15 1937.0 16 2146.0 17 2085.5 18 2025.0 19 2354.5 20 2684.0 21 3036.5 22 3389.0 23 3744.0 24 4099.0 25 4887.5 26 6965.5 27 8255.0 28 10006.0 29 11757.0 30 13635.5 31 15514.0 32 17793.0 33 20072.0 34 21085.0 35 22098.0 36 24432.5 37 26767.0 38 29778.5 39 32790.0 40 36329.0 41 39868.0 42 44609.0 43 49350.0 44 65079.5 45 80809.0 46 72712.5 47 64616.0 48 64866.5 49 65117.0 50 59734.5 51 54352.0 52 50491.5 53 46631.0 54 44930.0 55 43229.0 56 41751.0 57 40273.0 58 39071.5 59 37870.0 60 36221.0 61 34572.0 62 32302.0 63 30032.0 64 25022.5 65 20013.0 66 17254.5 67 14496.0 68 12584.0 69 10672.0 70 8966.5 71 7261.0 72 6057.0 73 4853.0 74 4007.5 75 2447.5 76 1733.0 77 1367.5 78 1002.0 79 764.5 80 527.0 81 380.5 82 234.0 83 166.5 84 99.0 85 74.0 86 49.0 87 47.0 88 45.0 89 40.5 90 36.0 91 24.5 92 13.0 93 7.0 94 1.0 95 2.0 96 3.0 97 2.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 827524.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.113433541312773 #Duplication Level Percentage of deduplicated Percentage of total 1 73.51046406343183 22.13652474165215 2 9.136862449854714 5.502846003196323 3 3.71874309387535 3.3595236904389356 4 2.223900741030715 2.6787714867001866 5 1.465938357112265 2.2072218646280715 6 1.1099692811255117 2.005499170804309 7 0.8291508432279061 1.7478005169286894 8 0.6867444063735242 1.6544185632957933 9 0.5197716624016572 1.4086898471150964 >10 6.428369747779797 45.28926065671735 >50 0.32183069076993637 5.90336408889497 >100 0.04147508226240406 2.220141396525032 >500 0.003987988679077313 0.7779561836257455 >1k 0.001595195471630925 1.0234242971377898 >5k 0.0011963966037231938 2.084557492339563 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC 6020 0.7274713482630111 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG 6011 0.7263837665131162 No Hit GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC 5327 0.6437275535211063 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3887 0.4697144735379276 No Hit GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC 2373 0.28675905472227997 No Hit GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT 1217 0.147065221069117 No Hit GAATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT 1045 0.12628032540445958 No Hit TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC 897 0.10839564773952176 No Hit GAATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC 847 0.1023535269067725 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.625272499649557E-4 0.0 0.0 0.0989699392404329 0.0 2 3.625272499649557E-4 0.0 0.0 0.6004659683586216 0.0 3 3.625272499649557E-4 0.0 0.0 0.7558693161769326 0.0 4 3.625272499649557E-4 0.0 0.0 1.1241970021413277 0.0 5 3.625272499649557E-4 0.0 0.0 2.3511100584393927 0.0 6 3.625272499649557E-4 0.0 0.0 2.8342380402260234 0.0 7 3.625272499649557E-4 0.0 0.0 3.302985774430711 0.0 8 3.625272499649557E-4 0.0 0.0 4.010638966362305 0.0 9 3.625272499649557E-4 0.0 0.0 4.266462362420909 0.0 10 3.625272499649557E-4 0.0 0.0 5.608296556957865 0.0 11 3.625272499649557E-4 0.0 0.0 6.30434887689058 0.0 12 3.625272499649557E-4 0.0 0.0 7.6969368864226295 0.0 13 3.625272499649557E-4 0.0 0.0 8.032999647140143 0.0 14 3.625272499649557E-4 0.0 0.0 8.213417375206037 0.0 15 3.625272499649557E-4 0.0 0.0 8.513952465426984 0.0 16 3.625272499649557E-4 0.0 0.0 8.947776741218382 0.0 17 3.625272499649557E-4 0.0 0.0 9.430783880588358 0.0 18 3.625272499649557E-4 0.0 0.0 9.945572575538595 0.0 19 3.625272499649557E-4 0.0 0.0 10.289610935755338 0.0 20 3.625272499649557E-4 0.0 0.0 10.627123805472712 0.0 21 3.625272499649557E-4 0.0 0.0 11.035812858599872 0.0 22 3.625272499649557E-4 0.0 0.0 11.450544032559781 0.0 23 3.625272499649557E-4 0.0 0.0 11.842798517021862 0.0 24 3.625272499649557E-4 0.0 0.0 12.172939999323283 0.0 25 3.625272499649557E-4 0.0 0.0 12.468158023211412 0.0 26 3.625272499649557E-4 0.0 0.0 12.759992459433201 0.0 27 3.625272499649557E-4 0.0 0.0 13.090738153817895 0.0 28 3.625272499649557E-4 0.0 0.0 13.392239983372084 0.0 29 3.625272499649557E-4 0.0 0.0 13.710297224008004 0.0 30 3.625272499649557E-4 0.0 0.0 14.095663690720752 0.0 31 4.8336966661994093E-4 0.0 0.0 14.45021534118648 0.0 32 4.8336966661994093E-4 0.0 0.0 14.790507586486918 0.0 33 4.8336966661994093E-4 0.0 0.0 15.125845292704502 0.0 34 6.042120832749261E-4 0.0 0.0 15.478100937253783 0.0 35 6.042120832749261E-4 0.0 0.0 15.845824411134904 0.0 36 6.042120832749261E-4 0.0 0.0 16.169077875686988 0.0 37 6.042120832749261E-4 0.0 0.0 16.53100091356867 0.0 38 6.042120832749261E-4 0.0 0.0 16.89582416945007 0.0 39 6.042120832749261E-4 0.0 0.0 17.394057453318574 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAGAC 20 7.0321414E-4 45.0 45 ACGTCCA 20 7.0321414E-4 45.0 24 TCGACGT 20 7.0321414E-4 45.0 26 TTCGAAA 20 7.0321414E-4 45.0 35 TACGTCC 20 7.0321414E-4 45.0 23 TACGTAG 25 3.889856E-5 45.0 1 CCCGATA 20 7.0321414E-4 45.0 23 AATAGCG 30 2.164763E-6 44.999996 1 TACGGCT 810 0.0 40.555557 7 CGTTTTT 1660 0.0 39.849396 1 CACGGGA 240 0.0 37.499996 4 TAGCGCG 30 1.1396458E-4 37.499996 1 GCCGATG 30 1.1396458E-4 37.499996 9 AACGTAA 55 2.746674E-9 36.818184 39 ACGTAAC 55 2.746674E-9 36.818184 40 ACGGGAG 370 0.0 36.486485 5 ATGGGCG 105 0.0 36.42857 5 AGGGTAC 210 0.0 36.42857 6 ACACGTT 25 0.0021067685 36.0 44 GCGCGAC 150 0.0 36.0 9 >>END_MODULE