##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936651.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	827524
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.147160686578275	31.0	31.0	33.0	28.0	34.0
2	31.17417017512483	31.0	31.0	34.0	28.0	34.0
3	30.98737680115622	31.0	31.0	34.0	28.0	34.0
4	34.95255243352459	35.0	35.0	37.0	32.0	37.0
5	35.17491214756309	35.0	35.0	37.0	33.0	37.0
6	35.13854462227077	36.0	35.0	37.0	32.0	37.0
7	31.064674861393748	35.0	35.0	37.0	0.0	37.0
8	33.03412348161503	35.0	35.0	37.0	17.0	37.0
9	36.2307123418777	39.0	35.0	39.0	32.0	39.0
10	36.085726818799216	37.0	35.0	39.0	32.0	39.0
11	36.34892522754627	37.0	35.0	39.0	32.0	39.0
12	36.42125908130761	38.0	35.0	39.0	32.0	39.0
13	36.34012789961379	38.0	35.0	39.0	32.0	39.0
14	37.38925275883237	39.0	36.0	40.0	32.0	41.0
15	37.533789956545064	39.0	36.0	41.0	32.0	41.0
16	37.511020828398934	39.0	36.0	41.0	32.0	41.0
17	37.39613835973337	39.0	36.0	40.0	32.0	41.0
18	37.32933425495816	39.0	36.0	40.0	32.0	41.0
19	37.14635829293169	39.0	36.0	40.0	32.0	41.0
20	37.106986625161326	39.0	35.0	40.0	32.0	41.0
21	37.155201540982496	39.0	35.0	40.0	32.0	41.0
22	37.269184942068144	39.0	35.0	40.0	32.0	41.0
23	37.3275735809475	39.0	35.0	40.0	32.0	41.0
24	37.24706594612362	39.0	35.0	41.0	32.0	41.0
25	36.95251255552709	39.0	35.0	40.0	31.0	41.0
26	36.912675644452605	39.0	35.0	40.0	31.0	41.0
27	36.9462082066502	39.0	35.0	40.0	31.0	41.0
28	36.93923318236087	39.0	35.0	40.0	31.0	41.0
29	36.922678979703306	39.0	35.0	40.0	31.0	41.0
30	36.710544950962145	39.0	35.0	40.0	31.0	41.0
31	36.721200835262785	39.0	35.0	40.0	31.0	41.0
32	36.58683252691161	39.0	35.0	40.0	31.0	41.0
33	36.45381161150613	39.0	35.0	40.0	30.0	41.0
34	36.38248920877219	39.0	35.0	40.0	30.0	41.0
35	36.33918895403638	39.0	35.0	40.0	30.0	41.0
36	36.26915593988815	39.0	35.0	40.0	30.0	41.0
37	36.195155669201135	39.0	35.0	40.0	30.0	41.0
38	36.07000642881657	38.0	35.0	40.0	30.0	41.0
39	36.03179001454943	38.0	35.0	40.0	29.0	41.0
40	35.89759209400573	38.0	35.0	40.0	29.0	41.0
41	35.75553699953113	38.0	35.0	40.0	28.0	41.0
42	35.74898371527593	38.0	35.0	40.0	29.0	41.0
43	35.70475176550771	38.0	35.0	40.0	28.0	41.0
44	35.53097916193367	38.0	34.0	40.0	28.0	41.0
45	35.453785026174465	38.0	34.0	40.0	28.0	41.0
46	35.38075028639653	38.0	34.0	40.0	27.0	41.0
47	35.322822057124625	38.0	34.0	40.0	27.0	41.0
48	35.254705603704544	38.0	34.0	40.0	27.0	41.0
49	35.12738482509268	37.0	34.0	40.0	27.0	41.0
50	34.966520608465736	37.0	34.0	40.0	26.0	41.0
51	33.29047133376192	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	30.0
9	70.0
10	76.0
11	53.0
12	47.0
13	30.0
14	34.0
15	64.0
16	122.0
17	221.0
18	433.0
19	823.0
20	1341.0
21	1942.0
22	2828.0
23	3968.0
24	5435.0
25	7224.0
26	9086.0
27	10837.0
28	12561.0
29	14887.0
30	18238.0
31	23641.0
32	31195.0
33	43083.0
34	65027.0
35	71321.0
36	84385.0
37	124380.0
38	163156.0
39	130974.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.54671526143048	20.449799643273188	22.500495453908282	11.502989641388044
2	32.56775634301845	26.43101589802834	24.25754419207177	16.743683566881444
3	33.05753065772111	25.436724493791118	25.747893716677705	15.757851131810074
4	28.663337860895876	24.868765135512685	27.375399384187045	19.09249761940439
5	27.348209840439676	29.85677756778051	23.36306862399157	19.431943967788246
6	25.517568070533304	36.145537772922594	24.402313407224444	13.934580749319657
7	67.50366152522464	18.15753984174477	10.707000642881656	3.6317979901489257
8	77.68511849807376	8.279276492283003	9.526732759412415	4.508872250230809
9	70.83685790381911	8.743794741904766	11.592775556962698	8.82657179731343
10	41.2614014820114	25.246639372392828	18.79087494743355	14.701084198162228
11	32.47494936702742	25.047853596995374	24.176096403246312	18.301100632730893
12	28.50600103441109	23.251893600668982	28.09972882961703	20.1423765353029
13	25.030452288997058	24.596507170789003	30.492166994552424	19.880873545661515
14	20.944770181892007	27.7985895273128	29.569414300974955	21.687225989820234
15	19.92800208815696	25.40893073796047	34.19622874986103	20.46683842402154
16	22.556566335236198	25.99417056182056	29.97290713018595	21.476355972757286
17	23.258660776001662	25.725779554429838	28.60352086465166	22.412038804916836
18	24.14528158699929	25.890971137997205	28.5765730057376	21.387174269265905
19	25.38053277004655	26.755961156413587	26.344734412536674	21.518771661003186
20	25.883478908164598	26.210599330049643	28.844843170711666	19.061078591074097
21	24.7696743538556	27.863361062639875	28.183714309192244	19.183250274312286
22	23.738646854955263	24.207032061909985	28.514580845993592	23.539740237141157
23	23.209477912423086	27.07015143971655	29.122176516934857	20.598194130925506
24	23.384941101406124	24.897525630676572	29.018010353778255	22.699522914139045
25	22.851059304624396	28.134168918363695	26.856381204653886	22.158390572358023
26	21.868247929969403	27.18374331137224	27.554487845669733	23.393520912988627
27	22.672091685558364	27.554487845669733	27.698532006322473	22.074888462449426
28	20.05234893489494	27.427603308181997	29.88396711152788	22.63608064539518
29	22.85649721337387	28.14299041477951	26.964414325143437	22.036098046703177
30	22.22159115626858	28.205465944190138	28.692581725726384	20.880361173814897
31	23.95592152010093	26.642973496841176	26.77191235541205	22.629192627645843
32	25.455092541122674	28.423828191085697	26.16612932072061	19.95494994707102
33	23.64161039438131	27.832787931226164	25.66330402501921	22.86229764937331
34	22.384728418752807	28.01344734412537	27.809223659978443	21.792600577143382
35	22.3299868040081	28.85608151546058	26.227758953214654	22.58617272731667
36	23.347479952243077	30.19537801924778	26.301714512207504	20.155427516301643
37	23.34349215249346	29.3231374558321	27.039578308302843	20.2937920833716
38	22.466780419661543	29.21848792300888	26.615904795510463	21.69882686181911
39	24.702969339862047	26.241414146296666	25.815927997254462	23.239688516586828
40	23.637501752215044	27.258907294531635	27.710132878321353	21.393458074931964
41	21.28349147577593	27.2300259569511	27.233409544617437	24.253073022655535
42	22.46279261991193	27.423857193265693	27.815869992894466	22.29748019392791
43	24.05851673184101	26.136522928640137	26.960909895060446	22.844050444458407
44	22.89661689570333	25.457388547039116	27.784088437314203	23.86190611994335
45	22.0124129330388	26.083110580478632	26.949792392728188	24.95468409375438
46	23.24234704975324	27.383374983686277	25.967343545323157	23.40693442123733
47	21.04688202396547	26.536148800518173	29.87997931177827	22.536989863738093
48	22.120446053528354	27.380837292936523	27.33201695660791	23.166699696927218
49	21.992836461540694	25.689768514266653	29.584277918223517	22.733117105969132
50	20.973651519472547	26.293859755124927	28.720858851223653	24.011629874178876
51	21.147181229789105	25.49629980520202	26.996437565557013	26.360081399451857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	340.0
1	969.0
2	1598.0
3	4322.0
4	7046.0
5	4748.0
6	2450.0
7	2257.5
8	2065.0
9	2081.5
10	2098.0
11	2052.5
12	2007.0
13	1867.5
14	1728.0
15	1937.0
16	2146.0
17	2085.5
18	2025.0
19	2354.5
20	2684.0
21	3036.5
22	3389.0
23	3744.0
24	4099.0
25	4887.5
26	6965.5
27	8255.0
28	10006.0
29	11757.0
30	13635.5
31	15514.0
32	17793.0
33	20072.0
34	21085.0
35	22098.0
36	24432.5
37	26767.0
38	29778.5
39	32790.0
40	36329.0
41	39868.0
42	44609.0
43	49350.0
44	65079.5
45	80809.0
46	72712.5
47	64616.0
48	64866.5
49	65117.0
50	59734.5
51	54352.0
52	50491.5
53	46631.0
54	44930.0
55	43229.0
56	41751.0
57	40273.0
58	39071.5
59	37870.0
60	36221.0
61	34572.0
62	32302.0
63	30032.0
64	25022.5
65	20013.0
66	17254.5
67	14496.0
68	12584.0
69	10672.0
70	8966.5
71	7261.0
72	6057.0
73	4853.0
74	4007.5
75	2447.5
76	1733.0
77	1367.5
78	1002.0
79	764.5
80	527.0
81	380.5
82	234.0
83	166.5
84	99.0
85	74.0
86	49.0
87	47.0
88	45.0
89	40.5
90	36.0
91	24.5
92	13.0
93	7.0
94	1.0
95	2.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	827524.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.113433541312773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51046406343183	22.13652474165215
2	9.136862449854714	5.502846003196323
3	3.71874309387535	3.3595236904389356
4	2.223900741030715	2.6787714867001866
5	1.465938357112265	2.2072218646280715
6	1.1099692811255117	2.005499170804309
7	0.8291508432279061	1.7478005169286894
8	0.6867444063735242	1.6544185632957933
9	0.5197716624016572	1.4086898471150964
>10	6.428369747779797	45.28926065671735
>50	0.32183069076993637	5.90336408889497
>100	0.04147508226240406	2.220141396525032
>500	0.003987988679077313	0.7779561836257455
>1k	0.001595195471630925	1.0234242971377898
>5k	0.0011963966037231938	2.084557492339563
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC	6020	0.7274713482630111	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG	6011	0.7263837665131162	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	5327	0.6437275535211063	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3887	0.4697144735379276	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	2373	0.28675905472227997	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT	1217	0.147065221069117	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT	1045	0.12628032540445958	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	897	0.10839564773952176	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC	847	0.1023535269067725	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.625272499649557E-4	0.0	0.0	0.0989699392404329	0.0
2	3.625272499649557E-4	0.0	0.0	0.6004659683586216	0.0
3	3.625272499649557E-4	0.0	0.0	0.7558693161769326	0.0
4	3.625272499649557E-4	0.0	0.0	1.1241970021413277	0.0
5	3.625272499649557E-4	0.0	0.0	2.3511100584393927	0.0
6	3.625272499649557E-4	0.0	0.0	2.8342380402260234	0.0
7	3.625272499649557E-4	0.0	0.0	3.302985774430711	0.0
8	3.625272499649557E-4	0.0	0.0	4.010638966362305	0.0
9	3.625272499649557E-4	0.0	0.0	4.266462362420909	0.0
10	3.625272499649557E-4	0.0	0.0	5.608296556957865	0.0
11	3.625272499649557E-4	0.0	0.0	6.30434887689058	0.0
12	3.625272499649557E-4	0.0	0.0	7.6969368864226295	0.0
13	3.625272499649557E-4	0.0	0.0	8.032999647140143	0.0
14	3.625272499649557E-4	0.0	0.0	8.213417375206037	0.0
15	3.625272499649557E-4	0.0	0.0	8.513952465426984	0.0
16	3.625272499649557E-4	0.0	0.0	8.947776741218382	0.0
17	3.625272499649557E-4	0.0	0.0	9.430783880588358	0.0
18	3.625272499649557E-4	0.0	0.0	9.945572575538595	0.0
19	3.625272499649557E-4	0.0	0.0	10.289610935755338	0.0
20	3.625272499649557E-4	0.0	0.0	10.627123805472712	0.0
21	3.625272499649557E-4	0.0	0.0	11.035812858599872	0.0
22	3.625272499649557E-4	0.0	0.0	11.450544032559781	0.0
23	3.625272499649557E-4	0.0	0.0	11.842798517021862	0.0
24	3.625272499649557E-4	0.0	0.0	12.172939999323283	0.0
25	3.625272499649557E-4	0.0	0.0	12.468158023211412	0.0
26	3.625272499649557E-4	0.0	0.0	12.759992459433201	0.0
27	3.625272499649557E-4	0.0	0.0	13.090738153817895	0.0
28	3.625272499649557E-4	0.0	0.0	13.392239983372084	0.0
29	3.625272499649557E-4	0.0	0.0	13.710297224008004	0.0
30	3.625272499649557E-4	0.0	0.0	14.095663690720752	0.0
31	4.8336966661994093E-4	0.0	0.0	14.45021534118648	0.0
32	4.8336966661994093E-4	0.0	0.0	14.790507586486918	0.0
33	4.8336966661994093E-4	0.0	0.0	15.125845292704502	0.0
34	6.042120832749261E-4	0.0	0.0	15.478100937253783	0.0
35	6.042120832749261E-4	0.0	0.0	15.845824411134904	0.0
36	6.042120832749261E-4	0.0	0.0	16.169077875686988	0.0
37	6.042120832749261E-4	0.0	0.0	16.53100091356867	0.0
38	6.042120832749261E-4	0.0	0.0	16.89582416945007	0.0
39	6.042120832749261E-4	0.0	0.0	17.394057453318574	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGAC	20	7.0321414E-4	45.0	45
ACGTCCA	20	7.0321414E-4	45.0	24
TCGACGT	20	7.0321414E-4	45.0	26
TTCGAAA	20	7.0321414E-4	45.0	35
TACGTCC	20	7.0321414E-4	45.0	23
TACGTAG	25	3.889856E-5	45.0	1
CCCGATA	20	7.0321414E-4	45.0	23
AATAGCG	30	2.164763E-6	44.999996	1
TACGGCT	810	0.0	40.555557	7
CGTTTTT	1660	0.0	39.849396	1
CACGGGA	240	0.0	37.499996	4
TAGCGCG	30	1.1396458E-4	37.499996	1
GCCGATG	30	1.1396458E-4	37.499996	9
AACGTAA	55	2.746674E-9	36.818184	39
ACGTAAC	55	2.746674E-9	36.818184	40
ACGGGAG	370	0.0	36.486485	5
ATGGGCG	105	0.0	36.42857	5
AGGGTAC	210	0.0	36.42857	6
ACACGTT	25	0.0021067685	36.0	44
GCGCGAC	150	0.0	36.0	9
>>END_MODULE