FastQCFastQC Report
Sat 14 Jan 2017
SRR2936649.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936649.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582561
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23220.3985848692239954No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC10170.17457399310973443No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC7390.12685366854286506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT351.2105556E-745.04
GCTAGCG253.888429E-545.01
CGTGTTA207.030419E-445.019
CGTGTAC650.041.5384618
CAACGAG2250.040.014
AGTAGCG451.9255822E-840.01
CGTTTTT12400.038.6491931
ACTACGG356.2429845E-638.5714262
ATCGCCT356.2429845E-638.57142631
AACGAGC2350.038.2978715
CACAACG2350.038.2978712
CGTTTTA950.037.8947371
TACGGGA1550.037.7419364
CATATCG301.1392309E-437.49999632
CGTGTTG301.1392309E-437.49999628
TGCAACG301.1392309E-437.4999961
CGAATAT1150.037.17391214
TCGTTGA552.744855E-936.8181824
ACATACG800.036.562517
ACGGGAG3450.036.521745
Produced by FastQC (version 0.11.5)