##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936648.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	648387
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15984589450436	31.0	31.0	33.0	28.0	34.0
2	31.211384558913117	31.0	31.0	34.0	28.0	34.0
3	30.98832178930176	31.0	31.0	34.0	28.0	34.0
4	34.959686113385985	35.0	35.0	37.0	32.0	37.0
5	35.20147381116524	35.0	35.0	37.0	33.0	37.0
6	35.18627301287657	36.0	35.0	37.0	32.0	37.0
7	31.088592152526193	35.0	35.0	37.0	0.0	37.0
8	33.15311071936976	36.0	35.0	37.0	17.0	37.0
9	36.45181504255946	39.0	35.0	39.0	32.0	39.0
10	36.08468245045012	37.0	35.0	39.0	32.0	39.0
11	36.393715481649075	38.0	35.0	39.0	32.0	39.0
12	36.52170540124956	38.0	35.0	39.0	32.0	39.0
13	36.549497445198625	39.0	35.0	39.0	32.0	39.0
14	37.58695501297836	39.0	36.0	41.0	33.0	41.0
15	37.77824509128036	39.0	37.0	41.0	33.0	41.0
16	37.67702622045167	39.0	36.0	41.0	33.0	41.0
17	37.59710173091071	39.0	36.0	41.0	33.0	41.0
18	37.51973898304562	39.0	36.0	40.0	33.0	41.0
19	37.31998482387833	39.0	36.0	40.0	32.0	41.0
20	37.34935462925691	39.0	36.0	40.0	32.0	41.0
21	37.329668855174454	39.0	36.0	40.0	32.0	41.0
22	37.41568538542568	39.0	36.0	40.0	32.0	41.0
23	37.46866454756187	39.0	36.0	41.0	33.0	41.0
24	37.439219170032715	39.0	36.0	41.0	32.0	41.0
25	37.08732130656537	39.0	35.0	40.0	32.0	41.0
26	37.105299766960165	39.0	35.0	40.0	32.0	41.0
27	37.14300718552346	39.0	35.0	40.0	32.0	41.0
28	37.13522325401342	39.0	35.0	40.0	32.0	41.0
29	37.12724345182738	39.0	35.0	41.0	32.0	41.0
30	36.909222424262055	39.0	35.0	40.0	31.0	41.0
31	36.92487202858786	39.0	35.0	40.0	31.0	41.0
32	36.795975243180386	39.0	35.0	40.0	31.0	41.0
33	36.70903642423429	39.0	35.0	40.0	31.0	41.0
34	36.65381785877879	39.0	35.0	40.0	30.0	41.0
35	36.62199272965066	39.0	35.0	40.0	30.0	41.0
36	36.590934118049866	39.0	35.0	40.0	30.0	41.0
37	36.527348635922685	39.0	35.0	40.0	30.0	41.0
38	36.41820394301551	39.0	35.0	40.0	30.0	41.0
39	36.38958985914277	39.0	35.0	40.0	30.0	41.0
40	36.3429757228322	39.0	35.0	40.0	30.0	41.0
41	36.2111300812632	39.0	35.0	40.0	30.0	41.0
42	36.2158448580863	39.0	35.0	40.0	30.0	41.0
43	36.146921514465895	39.0	35.0	40.0	30.0	41.0
44	35.978466563950235	38.0	35.0	40.0	29.0	41.0
45	35.85797062556775	38.0	35.0	40.0	29.0	41.0
46	35.776226235257646	38.0	35.0	40.0	29.0	41.0
47	35.75086021157117	38.0	35.0	40.0	29.0	41.0
48	35.694915228096804	38.0	35.0	40.0	29.0	41.0
49	35.57044326922039	38.0	34.0	40.0	28.0	41.0
50	35.41490344501047	38.0	34.0	40.0	27.0	41.0
51	33.79324539202668	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	19.0
9	34.0
10	60.0
11	43.0
12	33.0
13	24.0
14	31.0
15	41.0
16	92.0
17	158.0
18	285.0
19	516.0
20	809.0
21	1255.0
22	2046.0
23	2788.0
24	3828.0
25	5218.0
26	6515.0
27	7620.0
28	8804.0
29	10568.0
30	13100.0
31	16791.0
32	22064.0
33	30737.0
34	46825.0
35	53910.0
36	65009.0
37	100405.0
38	136812.0
39	111938.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09216563564661	21.027565327497314	24.111371757916185	11.768897278939892
2	33.79201001870796	22.954346709603985	25.421083396181604	17.832559875506448
3	34.01240308642832	22.89065018268411	26.276128299919648	16.820818430967925
4	28.947680937464813	25.97260586655809	25.707949110639174	19.371764085337922
5	25.841357090749817	29.974845269877402	24.95469526686994	19.22910237250284
6	26.511790026635328	35.385657022734875	24.3357747764838	13.766778174145996
7	70.29397566576752	18.26424650710147	7.7836230522820475	3.6581547748489713
8	82.3551366699209	5.654956067903891	7.949727554685706	4.040179707489509
9	75.64849387788466	8.551374410652897	10.496663258208446	5.303468453253998
10	38.79627444720514	28.68148189275849	18.25730620755814	14.264937452478227
11	29.261845163459476	28.15324798307184	25.07483956340889	17.510067290059794
12	26.127143203056196	25.989571043219563	29.203855105053	18.679430648671243
13	24.897939039493387	26.635635816264053	29.76509399479015	18.70133114945241
14	20.867938438000763	29.776969618453176	29.52264619740988	19.83244574613618
15	20.61731651004724	28.239153468530372	32.181089380262094	18.962440641160295
16	23.042719857122368	27.21399411154141	30.523437391557817	19.2198486397784
17	23.429525885003862	25.949779992504478	28.463556487097986	22.157137635393678
18	25.57546650380097	25.46920280634868	28.976984424425535	19.978346265424815
19	24.42970016363684	27.904785259420684	27.273526458735294	20.391988118207184
20	25.815138181363906	26.618516410723842	27.902163368482096	19.664182039430155
21	24.736924090088174	27.654471789224644	28.612078897325212	18.996525223361974
22	23.773764742352945	25.72691926272427	28.201521622117653	22.29779437280513
23	21.919933619890593	27.59725287521187	28.72482020768461	21.75799329721293
24	21.785754495386243	26.165546193862614	30.711905081378866	21.336794229372273
25	21.51600818646888	28.385825132212705	28.67161124451909	21.42655543679932
26	21.818451017679255	28.859153561067696	27.982516614305965	21.339878806947084
27	22.16053067072597	28.41404901702224	27.827979277807852	21.597441034443936
28	19.718162146989375	29.085561555058938	29.567681030002145	21.628595267949542
29	20.44859011670499	28.073820110520415	29.79964126362805	21.677948509146542
30	21.391391252446457	27.092924441730016	29.409133742656778	22.106550563166753
31	22.661774526633014	28.35744701852443	26.85386967968204	22.126908775160516
32	21.7016997564726	29.549636251189494	27.75132752507376	20.997336467264148
33	21.928878894857544	27.867924557401675	27.490218033365878	22.7129785143749
34	20.151545296250543	29.300402383144636	28.77664111094146	21.771411209663363
35	20.632739397921306	29.377054135878726	27.98004895224611	22.010157513953857
36	21.724679859404954	29.46265116357978	27.299128452606237	21.513540524409034
37	20.822440918772276	29.956183575549787	26.748685584380933	22.472689921297004
38	21.11778922156058	29.92518357092292	27.033854781172355	21.92317242634414
39	21.481460917630983	28.57244207548887	27.646606116408872	22.299490890471276
40	22.834356641943778	27.447650862833463	28.728213243016903	20.98977925220586
41	20.064405979762086	28.48931270984767	27.662491690919154	23.783789619471086
42	21.77727190705551	28.347884828042513	27.25656128207382	22.618281982828154
43	21.91191371819608	27.489138431214695	27.545740429712502	23.053207420876728
44	20.929784218375755	28.20954152381217	27.313317509450375	23.547356748361704
45	21.071983244574614	28.27447188176197	27.065317472435446	23.58822740122797
46	21.736092796431763	28.644466961860743	26.864048785678925	22.755391456028576
47	20.606674717414137	28.713869957294026	28.7027654780247	21.976689847267142
48	20.684714530056894	28.163427089068723	28.45970076512947	22.692157615744918
49	21.531431074342947	27.246227947198204	29.168845149578875	22.053495828879974
50	20.24423685237366	27.94982009201295	28.66436248721828	23.14158056839511
51	20.032326372984034	26.878854758038024	27.751019067316278	25.337799801661664
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	224.0
1	581.0
2	938.0
3	2556.5
4	4175.0
5	2911.5
6	1648.0
7	1614.5
8	1581.0
9	1536.0
10	1491.0
11	1519.5
12	1548.0
13	1542.0
14	1536.0
15	1655.0
16	1774.0
17	1764.5
18	1755.0
19	1702.0
20	1649.0
21	1946.0
22	2243.0
23	2751.0
24	3259.0
25	3948.5
26	5616.5
27	6595.0
28	7962.0
29	9329.0
30	10747.0
31	12165.0
32	14266.0
33	16367.0
34	18482.0
35	20597.0
36	22829.5
37	25062.0
38	28076.5
39	31091.0
40	33682.5
41	36274.0
42	40323.5
43	44373.0
44	49674.0
45	54975.0
46	58203.0
47	61431.0
48	59659.0
49	57887.0
50	52437.0
51	46987.0
52	43967.5
53	40948.0
54	37449.0
55	33950.0
56	31675.5
57	29401.0
58	27587.5
59	25774.0
60	23211.0
61	20648.0
62	18414.5
63	16181.0
64	13712.5
65	11244.0
66	9097.5
67	6951.0
68	5668.5
69	4386.0
70	3647.5
71	2909.0
72	2326.0
73	1743.0
74	1491.5
75	963.0
76	686.0
77	559.0
78	432.0
79	302.0
80	172.0
81	116.5
82	61.0
83	50.5
84	40.0
85	28.5
86	17.0
87	10.5
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	648387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.96427414582476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.22072723944963	24.46632400023701
2	9.296562607111916	6.1290887678932116
3	3.7009745377269483	3.659998178050445
4	2.198855810896662	2.8993474303014946
5	1.4464800093073558	2.3841081786631415
6	1.044684362074062	2.066235702435926
7	0.7874801760591845	1.8171098685612115
8	0.6164868058584221	1.6257632060480693
9	0.501341316455736	1.487371733664801
>10	5.813729007608486	44.189276844075806
>50	0.33744984674896766	6.716293363496978
>100	0.03337416066564149	1.880195423362033
>500	9.270600184900417E-4	0.17495520193007136
>1k	9.270600184900417E-4	0.5039321012798038
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2115	0.3261940785364296	No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1183	0.1824527635501637	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10718907072473692	0.0
2	0.0	0.0	0.0	0.538721473441016	0.0
3	0.0	0.0	0.0	0.7091443844494106	0.0
4	0.0	0.0	0.0	0.9699454183998137	0.0
5	0.0	0.0	0.0	1.6369853189530328	0.0
6	0.0	0.0	0.0	2.0444580165857738	0.0
7	0.0	0.0	0.0	2.418154589774317	0.0
8	0.0	0.0	0.0	3.1277616608599494	0.0
9	0.0	0.0	0.0	3.368821398331552	0.0
10	0.0	0.0	0.0	4.010567762771307	0.0
11	0.0	0.0	0.0	4.846025598909294	0.0
12	0.0	0.0	0.0	5.581697350502092	0.0
13	0.0	0.0	0.0	5.937040687120501	0.0
14	0.0	0.0	0.0	6.086642699498911	0.0
15	0.0	0.0	0.0	6.323075570608294	0.0
16	0.0	0.0	0.0	6.860254755261904	0.0
17	0.0	0.0	0.0	7.505702612791435	0.0
18	0.0	0.0	0.0	8.222249983420395	0.0
19	0.0	0.0	0.0	8.63064805432558	0.0
20	0.0	0.0	0.0	9.043827220471725	0.0
21	0.0	0.0	0.0	9.552011375922095	0.0
22	0.0	0.0	0.0	10.102300015268659	0.0
23	0.0	0.0	0.0	10.65937472527981	0.0
24	0.0	0.0	0.0	11.098772800811862	0.0
25	0.0	0.0	0.0	11.490051466176835	0.0
26	0.0	0.0	0.0	11.868529134606339	0.0
27	1.5422887874062867E-4	0.0	0.0	12.242842623309844	0.0
28	3.0845775748125734E-4	0.0	0.0	12.632887457644895	0.0
29	3.0845775748125734E-4	0.0	0.0	13.053006923334367	0.0
30	3.0845775748125734E-4	0.0	0.0	13.536360229307498	0.0
31	3.0845775748125734E-4	0.0	0.0	13.955554321724525	0.0
32	3.0845775748125734E-4	0.0	0.0	14.383076773593547	0.0
33	3.0845775748125734E-4	0.0	0.0	14.815071862946048	0.0
34	3.0845775748125734E-4	0.0	0.0	15.231181377788266	0.0
35	3.0845775748125734E-4	0.0	0.0	15.66147994947462	0.0
36	3.0845775748125734E-4	0.0	0.0	16.076355633286912	0.0
37	3.0845775748125734E-4	0.0	0.0	16.517141768727626	0.0
38	3.0845775748125734E-4	0.0	0.0	16.940500040870653	0.0
39	3.0845775748125734E-4	0.0	0.0	17.387301102582253	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGCT	25	3.888917E-5	45.000004	26
TATTACG	25	3.888917E-5	45.000004	1
CCGGATA	20	7.031009E-4	45.0	5
TCGGCGA	20	7.031009E-4	45.0	1
CCGTATG	20	7.031009E-4	45.0	45
CCATACG	20	7.031009E-4	45.0	2
GTCCGCG	20	7.031009E-4	45.0	1
ATCGCGT	20	7.031009E-4	45.0	20
CGACGGT	30	2.1640335E-6	44.999996	28
TTAAGCG	30	2.1640335E-6	44.999996	1
GCTTACG	55	6.002665E-11	40.909092	1
TACGGGA	140	0.0	40.17857	4
CGTTTTT	1180	0.0	39.47034	1
ACGATAG	40	3.4551158E-7	39.375	1
AATACGG	40	3.4551158E-7	39.375	2
ACGGGAG	295	0.0	38.898304	5
TCGGGCA	35	6.2440777E-6	38.571426	5
TACGCGG	35	6.2440777E-6	38.571426	2
AGACACG	230	0.0	38.152172	24
CGAATAT	65	9.094947E-12	38.07692	14
>>END_MODULE