FastQCFastQC Report
Sat 14 Jan 2017
SRR2936647.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936647.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081325
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192071.7762467343305666No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT16630.15379280049938732No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14180.13113541257253833No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC13870.12826855940628396No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC13140.12151758259542692No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA207.0331024E-445.01
ACCGGGT253.8906514E-545.04
TACGACG207.0331024E-445.01
CGTTTTT102800.043.7086521
CGTTTTA7600.041.447371
CGTTGAT451.9281288E-840.00000425
CGCGAAG451.9281288E-840.0000042
ATAGCGT403.4579716E-739.37521
TACGCGG601.5643309E-1037.4999962
TAAGACG301.1398772E-437.4999961
CGAATAT1700.037.05882314
CGGGTAC800.036.56256
ACAACGA2550.036.1764713
GTTTTTT127150.036.0106162
TACGAAT1750.036.00000412
TCGTTGA504.8785296E-836.024
CGGTATA250.002107053336.031
CACGTAA250.002107053336.043
AATCGTT504.8785296E-836.022
TTAAGCG458.6992804E-735.0000041
Produced by FastQC (version 0.11.5)