##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936647.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1081325
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14413612928583	31.0	31.0	33.0	28.0	34.0
2	31.23167872748711	31.0	31.0	34.0	28.0	34.0
3	30.853755346449958	31.0	31.0	34.0	27.0	34.0
4	34.78094005040113	35.0	35.0	37.0	32.0	37.0
5	35.13863731995468	35.0	35.0	37.0	33.0	37.0
6	35.160623309365825	36.0	35.0	37.0	32.0	37.0
7	31.033054817006914	35.0	35.0	37.0	0.0	37.0
8	33.13568168681941	37.0	35.0	37.0	17.0	37.0
9	36.54008554319932	39.0	35.0	39.0	32.0	39.0
10	36.32645226920676	37.0	35.0	39.0	32.0	39.0
11	36.560138718701594	38.0	35.0	39.0	32.0	39.0
12	36.675250271657454	39.0	35.0	39.0	32.0	39.0
13	36.72353177814256	39.0	35.0	39.0	33.0	39.0
14	37.7871380019883	40.0	37.0	41.0	33.0	41.0
15	37.95026749589624	40.0	37.0	41.0	33.0	41.0
16	37.8559443275611	40.0	37.0	41.0	33.0	41.0
17	37.73618477331052	39.0	37.0	41.0	33.0	41.0
18	37.550101033454325	39.0	36.0	40.0	33.0	41.0
19	37.26891822532541	39.0	36.0	40.0	32.0	41.0
20	37.15374841051488	39.0	35.0	40.0	32.0	41.0
21	37.106181767738654	39.0	35.0	40.0	32.0	41.0
22	37.17698471782304	39.0	35.0	40.0	32.0	41.0
23	37.219353108454904	39.0	35.0	40.0	33.0	41.0
24	37.142556585670356	39.0	35.0	40.0	32.0	41.0
25	36.807871823919726	38.0	35.0	40.0	31.0	41.0
26	36.80658451436894	39.0	35.0	40.0	32.0	41.0
27	36.83234180288073	39.0	35.0	40.0	32.0	41.0
28	36.82021408919613	39.0	35.0	40.0	32.0	41.0
29	36.774515524934685	39.0	35.0	40.0	31.0	41.0
30	36.47396481168936	38.0	35.0	40.0	31.0	41.0
31	36.34686888770721	38.0	35.0	40.0	30.0	41.0
32	36.03227984186068	38.0	35.0	40.0	30.0	41.0
33	35.69024206413428	38.0	35.0	40.0	27.0	41.0
34	35.39648116893626	38.0	35.0	40.0	25.0	41.0
35	35.17463389822671	38.0	35.0	40.0	23.0	41.0
36	35.06095392227129	38.0	34.0	40.0	23.0	41.0
37	34.948233879730886	38.0	34.0	40.0	23.0	41.0
38	34.811033685524706	38.0	34.0	40.0	22.0	41.0
39	34.7187607795991	38.0	34.0	40.0	21.0	41.0
40	34.6516079809493	38.0	34.0	40.0	21.0	41.0
41	34.47981411693986	38.0	34.0	40.0	20.0	41.0
42	34.46041106975239	38.0	34.0	40.0	20.0	41.0
43	34.40948003606686	38.0	34.0	40.0	20.0	41.0
44	34.22326590063117	38.0	34.0	40.0	18.0	41.0
45	34.14289505930225	38.0	33.0	40.0	19.0	41.0
46	34.07703419415994	38.0	33.0	40.0	19.0	41.0
47	34.04562920491064	38.0	33.0	40.0	19.0	41.0
48	33.96547014079948	38.0	33.0	40.0	19.0	41.0
49	33.857509074515065	37.0	33.0	40.0	18.0	41.0
50	33.72538783436987	37.0	33.0	40.0	18.0	41.0
51	32.270696599079834	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	43.0
9	94.0
10	101.0
11	80.0
12	58.0
13	40.0
14	67.0
15	94.0
16	190.0
17	411.0
18	747.0
19	1387.0
20	2235.0
21	3415.0
22	5180.0
23	7975.0
24	12382.0
25	18175.0
26	23209.0
27	24166.0
28	23121.0
29	22732.0
30	24748.0
31	29037.0
32	36765.0
33	49669.0
34	72328.0
35	84544.0
36	102327.0
37	155088.0
38	209521.0
39	171379.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.95611865072943	19.952835641458396	22.335283101750168	19.755762606062007
2	39.282084479689274	21.385707349779207	24.105056296673062	15.22715187385846
3	30.570550019651815	20.978799158439877	33.54310683652001	14.907543985388298
4	26.400480891498855	23.544262825699953	32.75490717406885	17.300349108732345
5	23.299655515224377	27.598548077589996	32.05428525189004	17.047511155295584
6	24.0767576815481	32.15203569694587	30.68725868725869	13.083947934247336
7	64.85376736873742	17.087369662219963	14.848172381106513	3.210690587936097
8	76.97482255566088	4.442975053753497	15.40452685362865	3.177675536956974
9	71.35449564192079	6.721291008716158	17.174947402492315	4.749265946870737
10	35.1949691350889	29.957228400342174	23.002242619009085	11.845559845559846
11	26.393544956419206	25.399209303400923	32.478394562226896	15.728851177952974
12	24.06871199685571	22.951009178554088	35.36244884747879	17.617829977111416
13	22.97995514761982	21.978036205581116	36.63875338126835	18.403255265530717
14	19.55683998797771	24.972510577300998	36.78653503803204	18.684114396689246
15	19.203662173722055	24.85589438882852	38.10029362125171	17.84014981619772
16	23.4842438674774	24.827410815434767	35.315192009802786	16.373153307285044
17	23.2756109402816	24.36557001826463	34.09927635077336	18.259542690680416
18	23.11460476729938	24.15471759184334	34.74746260375003	17.983215037107254
19	22.669225256051604	25.945021154601992	33.18604489861975	18.199708690726656
20	23.68066954893302	26.0866067093612	33.21785772085173	17.014866020854043
21	23.571544170346563	25.157098929554017	34.56796060388875	16.703396296210666
22	23.04801979053476	22.74505814625575	35.20763877650106	18.999283286708437
23	21.16079809492983	24.956419207916213	35.06577578433866	18.817006912815295
24	20.968626453656395	24.708205211199225	35.09093010889418	19.232238226250203
25	21.919126996971308	25.510369222944075	33.44942547337757	19.121078306707048
26	20.877904422814602	26.043973828404965	33.25022541789009	19.827896330890344
27	20.36751208008693	25.624580953922273	34.501190668855344	19.50671629713546
28	19.979192194761055	25.63123945159873	34.254826254826256	20.134742098813955
29	20.95114789725568	25.406700113286938	34.12535546667283	19.516796522784546
30	22.023073544031625	23.959586618269253	34.59071047094999	19.426629366749125
31	22.58821353432132	24.295193396989802	33.142394747185165	19.97419832150371
32	22.696599079832612	25.090791390192592	32.38915219753543	19.823457332439368
33	22.458002913092734	25.187062169098095	31.45613021062123	20.89880470718794
34	20.66714447552771	26.549834693547268	32.78366818486579	19.999352646059233
35	21.271310660532215	26.90606431923797	31.52299262478903	20.299632395440778
36	21.991815596606017	28.151480822139508	29.71086398631309	20.145839594941393
37	21.889441194830418	28.137793910249	29.49982660162301	20.472938293297574
38	22.107414514600144	27.892446766698264	29.384412641897672	20.615726076803924
39	21.724273460800404	26.76540355582272	29.679236122349895	21.83108686102698
40	23.313434906249277	25.886574341664165	30.794442004022844	20.005548748063717
41	21.10272119852958	26.97061475504589	30.57406422675884	21.352599819665688
42	21.819804406630755	27.30696136684161	30.22994936767392	20.643284858853722
43	21.80574757820267	26.766605784569858	30.118512010727578	21.309134626499894
44	21.712528610732203	26.435345525165882	29.96888077127598	21.88324509282593
45	21.65213973597207	25.714378193420114	30.045730931958474	22.58775113864934
46	21.55004277159966	26.39678172612304	29.771160381938827	22.282015120338475
47	20.866621968418375	27.002427577277878	30.869165144614247	21.2617853096895
48	20.969181328462767	26.351466950269348	31.235937391626013	21.443414329641875
49	21.344184218435718	26.033986081890276	31.08223707026102	21.539592629412986
50	20.269021801955933	26.316694795736712	31.494139134857697	21.920144267449658
51	20.504658636395163	25.58324278084757	30.994289413451092	22.917809169306175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	411.0
1	956.0
2	1501.0
3	17472.5
4	33444.0
5	22726.5
6	12009.0
7	11186.5
8	10364.0
9	10184.0
10	10004.0
11	9658.0
12	9312.0
13	9071.5
14	8831.0
15	8450.0
16	8069.0
17	7609.0
18	7149.0
19	7140.0
20	7131.0
21	6964.0
22	6797.0
23	7299.0
24	7801.0
25	8551.0
26	10485.5
27	11670.0
28	13629.5
29	15589.0
30	18324.5
31	21060.0
32	24240.0
33	27420.0
34	30713.0
35	34006.0
36	36092.0
37	38178.0
38	41800.5
39	45423.0
40	47236.0
41	49049.0
42	52903.0
43	56757.0
44	59823.0
45	62889.0
46	64730.0
47	66571.0
48	75026.0
49	83481.0
50	81166.5
51	78852.0
52	77788.0
53	76724.0
54	69636.0
55	62548.0
56	56964.0
57	51380.0
58	47525.5
59	43671.0
60	39568.0
61	35465.0
62	32005.0
63	28545.0
64	25085.0
65	21625.0
66	17897.0
67	14169.0
68	11387.0
69	8605.0
70	7342.0
71	6079.0
72	5088.5
73	4098.0
74	3223.0
75	1948.5
76	1549.0
77	1155.0
78	761.0
79	564.5
80	368.0
81	255.0
82	142.0
83	108.0
84	74.0
85	52.5
86	31.0
87	29.0
88	27.0
89	25.5
90	24.0
91	18.0
92	12.0
93	11.0
94	10.0
95	5.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1081325.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.1347838108158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72565217619847	26.279257188875167
2	8.960376819536355	6.47562558474783
3	4.251105161760567	4.608382979317836
4	2.569931622101687	3.7145569429289447
5	1.7429395971491082	3.1490372769146706
6	1.2833937615853557	2.78250936714219
7	1.0361003745463586	2.620748412837657
8	0.8342257619107171	2.411565404484548
9	0.6921383758703621	2.250924352132023
>10	5.806115368681489	39.70387660156419
>50	0.07612676060389745	1.7677735660063638
>100	0.01782087680303708	1.219280011361013
>500	0.0028004234861533084	0.71764021023102
>1k	0.0010183358131466576	0.5319056140950849
>5k	0.0	0.0
>10k+	2.545839532866644E-4	1.7669164873615177
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19207	1.7762467343305666	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	1663	0.15379280049938732	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1418	0.13113541257253833	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	1387	0.12826855940628396	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	1314	0.12151758259542692	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05696714678750607	0.0
2	0.0	0.0	0.0	0.23036552377869743	0.0
3	0.0	0.0	0.0	0.39035442628257	0.0
4	0.0	0.0	0.0	0.5925138140707003	0.0
5	0.0	0.0	0.0	1.1593184287795066	0.0
6	0.0	0.0	0.0	1.7965921438975332	0.0
7	0.0	0.0	0.0	2.264906480475343	0.0
8	0.0	0.0	0.0	2.979492751947842	0.0
9	0.0	0.0	0.0	3.4362471967262387	0.0
10	0.0	0.0	0.0	4.060851270432109	0.0
11	0.0	0.0	0.0	4.566897093843202	0.0
12	9.247913439530206E-5	0.0	0.0	5.017177999213927	0.0
13	9.247913439530206E-5	0.0	0.0	5.244029315885603	0.0
14	9.247913439530206E-5	0.0	0.0	5.409382008184403	0.0
15	9.247913439530206E-5	0.0	0.0	5.5568862275449105	0.0
16	9.247913439530206E-5	0.0	0.0	5.768755924444547	0.0
17	9.247913439530206E-5	0.0	0.0	5.99764178207292	0.0
18	9.247913439530206E-5	0.0	0.0	6.273322081705315	0.0
19	9.247913439530206E-5	0.0	0.0	6.460129933183826	0.0
20	9.247913439530206E-5	0.0	0.0	6.652579011860449	0.0
21	9.247913439530206E-5	0.0	0.0	6.858715002427577	0.0
22	9.247913439530206E-5	0.0	0.0	7.0994381892585485	0.0
23	9.247913439530206E-5	0.0	0.0	7.337479481192056	0.0
24	9.247913439530206E-5	0.0	0.0	7.532887892169329	0.0
25	9.247913439530206E-5	0.0	0.0	7.717106327884771	0.0
26	9.247913439530206E-5	0.0	0.0	7.895498578133308	0.0
27	9.247913439530206E-5	0.0	0.0	8.105981088017016	0.0
28	9.247913439530206E-5	0.0	0.0	8.30767808013317	0.0
29	9.247913439530206E-5	0.0	0.0	8.526761149515641	0.0
30	9.247913439530206E-5	0.0	0.0	8.844334497029108	0.0
31	9.247913439530206E-5	0.0	0.0	9.08191339329064	0.0
32	9.247913439530206E-5	0.0	0.0	9.344924051510878	0.0
33	9.247913439530206E-5	0.0	0.0	9.586941946223384	0.0
34	9.247913439530206E-5	0.0	0.0	9.845976001664624	0.0
35	9.247913439530206E-5	0.0	0.0	10.121656301297019	0.0
36	9.247913439530206E-5	0.0	0.0	10.381152752410237	0.0
37	9.247913439530206E-5	0.0	0.0	10.643238619286523	0.0
38	9.247913439530206E-5	0.0	0.0	10.95886990497769	0.0
39	9.247913439530206E-5	0.0	0.0	11.396573648070653	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	20	7.0331024E-4	45.0	1
ACCGGGT	25	3.8906514E-5	45.0	4
TACGACG	20	7.0331024E-4	45.0	1
CGTTTTT	10280	0.0	43.708652	1
CGTTTTA	760	0.0	41.44737	1
CGTTGAT	45	1.9281288E-8	40.000004	25
CGCGAAG	45	1.9281288E-8	40.000004	2
ATAGCGT	40	3.4579716E-7	39.375	21
TACGCGG	60	1.5643309E-10	37.499996	2
TAAGACG	30	1.1398772E-4	37.499996	1
CGAATAT	170	0.0	37.058823	14
CGGGTAC	80	0.0	36.5625	6
ACAACGA	255	0.0	36.17647	13
GTTTTTT	12715	0.0	36.010616	2
TACGAAT	175	0.0	36.000004	12
TCGTTGA	50	4.8785296E-8	36.0	24
CGGTATA	25	0.0021070533	36.0	31
CACGTAA	25	0.0021070533	36.0	43
AATCGTT	50	4.8785296E-8	36.0	22
TTAAGCG	45	8.6992804E-7	35.000004	1
>>END_MODULE