##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936646.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294510
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.047563749957558	31.0	31.0	33.0	28.0	34.0
2	31.116736273810737	31.0	31.0	34.0	28.0	34.0
3	30.724067094495943	31.0	30.0	34.0	27.0	34.0
4	34.708814641268546	35.0	35.0	37.0	32.0	37.0
5	35.07781739159961	35.0	35.0	37.0	33.0	37.0
6	35.11517435740722	35.0	35.0	37.0	32.0	37.0
7	31.277216393331297	35.0	35.0	37.0	0.0	37.0
8	33.25663984245017	36.0	35.0	37.0	17.0	37.0
9	36.40001018641133	39.0	35.0	39.0	32.0	39.0
10	36.18827204509185	37.0	35.0	39.0	32.0	39.0
11	36.5163491901803	38.0	35.0	39.0	32.0	39.0
12	36.53230111031883	38.0	35.0	39.0	32.0	39.0
13	36.36596380428509	39.0	35.0	39.0	32.0	39.0
14	37.39040100505925	39.0	36.0	41.0	32.0	41.0
15	37.66829988794947	39.0	36.0	41.0	33.0	41.0
16	37.57646599436352	39.0	36.0	41.0	33.0	41.0
17	37.515459576924385	39.0	36.0	41.0	33.0	41.0
18	37.339041119147055	39.0	36.0	40.0	32.0	41.0
19	37.05219517164103	38.0	36.0	40.0	32.0	41.0
20	36.93391395877899	38.0	35.0	40.0	32.0	41.0
21	36.88935520016298	38.0	35.0	40.0	32.0	41.0
22	36.945716614036876	38.0	35.0	40.0	32.0	41.0
23	36.999979627177346	38.0	35.0	40.0	32.0	41.0
24	36.913748259821396	38.0	35.0	40.0	32.0	41.0
25	36.615255848697835	38.0	35.0	40.0	31.0	41.0
26	36.59953482054939	38.0	35.0	40.0	31.0	41.0
27	36.64928525347187	38.0	35.0	40.0	31.0	41.0
28	36.65566873790364	38.0	35.0	40.0	31.0	41.0
29	36.6060099826831	38.0	35.0	40.0	31.0	41.0
30	36.336226274150285	38.0	35.0	40.0	31.0	41.0
31	36.190309327357305	38.0	35.0	40.0	30.0	41.0
32	35.83671861736443	38.0	35.0	40.0	29.0	41.0
33	35.53307867305015	38.0	35.0	40.0	27.0	41.0
34	35.32405011714373	38.0	35.0	40.0	25.0	41.0
35	35.11555465009677	38.0	34.0	40.0	24.0	41.0
36	35.03312960510679	38.0	34.0	40.0	24.0	41.0
37	34.9709585413059	38.0	34.0	40.0	23.0	41.0
38	34.83626362432515	38.0	34.0	40.0	23.0	41.0
39	34.818478150147705	38.0	34.0	40.0	23.0	41.0
40	34.74246375335303	38.0	34.0	40.0	23.0	41.0
41	34.62397202132355	38.0	34.0	40.0	22.0	41.0
42	34.615619164035174	38.0	34.0	40.0	22.0	41.0
43	34.545784523445725	38.0	34.0	40.0	22.0	41.0
44	34.31100471970392	38.0	34.0	40.0	20.0	41.0
45	34.20088282231503	38.0	33.0	40.0	20.0	41.0
46	34.136769549421075	38.0	33.0	40.0	20.0	41.0
47	34.10759566737971	38.0	33.0	40.0	20.0	41.0
48	34.044253166276185	37.0	33.0	40.0	20.0	41.0
49	33.943428066958674	37.0	33.0	40.0	20.0	41.0
50	33.74634817153917	37.0	33.0	40.0	18.0	41.0
51	32.138956232386	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	9.0
10	20.0
11	20.0
12	4.0
13	8.0
14	13.0
15	24.0
16	31.0
17	85.0
18	178.0
19	333.0
20	580.0
21	939.0
22	1328.0
23	2009.0
24	3230.0
25	4669.0
26	5953.0
27	6455.0
28	6237.0
29	6323.0
30	6871.0
31	8500.0
32	10832.0
33	15012.0
34	23433.0
35	26093.0
36	27579.0
37	40749.0
38	54834.0
39	42150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.14980815592	19.49950765678585	23.24165563138773	19.10902855590642
2	39.356218804115315	21.545957692438286	22.84302740144647	16.254796101999933
3	31.169739567417064	21.131031204373365	32.690570778581375	15.008658449628195
4	27.064276255475196	24.324810702522832	31.110318834674544	17.500594207327424
5	22.99548402431157	27.289735492852536	32.16053784251808	17.554242640317817
6	24.1910291670911	30.85803538080201	32.02743540117483	12.923500050932057
7	64.098672371057	16.284676241893315	15.77841159892703	3.8382397881226447
8	72.44372007741673	4.529897117245595	17.186173644358426	5.840209160979254
9	66.8391565651421	6.9668262537774615	19.81290957862212	6.381107602458321
10	34.97775966860209	23.29428542324539	26.79942956096567	14.928525347186852
11	27.707717904315643	24.583885097280227	31.776170588435026	15.932226409969102
12	26.830667889036025	21.122882075311534	35.74275915928152	16.303690876370922
13	20.879426844589318	26.47787851006757	35.889783029438725	16.752911615904384
14	17.182778173915995	29.21225085735629	35.41611490271977	18.188856066007943
15	16.20420359240773	25.88333163559811	41.38263556415742	16.529829207836748
16	17.564429051645106	25.304743472208074	38.97354928525347	18.157278190893347
17	18.999015313571697	23.432481070252283	34.80051611150725	22.76798750466877
18	20.352789378968456	24.016162439305965	36.693830430206106	18.937217751519473
19	20.910325625615428	25.469423788665917	35.26807239142983	18.35217819428882
20	23.162541170079116	24.93022308240807	34.19408509048928	17.71315065702353
21	21.258700893008726	26.15089470646158	35.16417099589148	17.426233404638214
22	19.057417405181486	23.652847101965975	34.84092220977216	22.44881328308037
23	17.83946215748192	26.341380598281894	34.30138195647007	21.51777528776612
24	19.244847373603612	24.20019693728566	36.95120708974228	19.603748599368444
25	17.512817900920172	25.307799395606263	35.056874129910696	22.122508573562868
26	17.5817459509015	28.225527146786188	35.21102848799701	18.981698414315304
27	18.47441513021629	27.713490204067774	35.95056195035822	17.861532715357715
28	17.44388985093885	25.546500967709072	37.760347696173305	19.24926148517877
29	16.70333774744491	25.72272588367118	37.95694543478999	19.61699093409392
30	19.723948253030457	25.259923262368	36.321347322671556	18.694781161929985
31	22.211130352110285	25.636820481477706	32.48480526977013	19.66724389664188
32	20.479440426471086	27.056127126413365	34.30579606804523	18.15863637907032
33	21.5429017690401	25.215442599572167	33.67491765984177	19.566737971545958
34	19.20036671080778	25.508132151709617	34.09663508879156	21.194866048691047
35	17.947777664595428	25.172659671997554	36.258531119486605	20.62103154392041
36	21.216257512478354	25.791314386608267	34.56690774506808	18.4255203558453
37	19.509694068113138	25.786900275033105	35.3010084547214	19.402397202132356
38	19.31649179993888	27.06223897320974	33.1608434348579	20.46042579199348
39	20.82577841159893	24.682014193066447	33.471528980340224	21.0206784149944
40	22.453566941699773	23.835862958812942	32.85966520661437	20.850904892872908
41	17.921292995144476	25.279277443889853	33.18155580455672	23.61787375640895
42	21.316423890530032	24.43007028623816	33.627381073647754	20.626124749584054
43	22.657634715289802	24.62836576007606	32.82638959627857	19.887609928355573
44	21.121863434178806	26.38789854334318	30.77926046653764	21.710977555940374
45	20.25533937727072	25.55465009677091	31.203694271841364	22.98631625411701
46	22.07157651692642	25.254830056704357	31.606397066313534	21.067196360055686
47	18.562697361719465	25.438864554684056	34.36589589487624	21.632542188720247
48	19.805439543648774	23.007368170860072	34.88336559030253	22.30382669518862
49	20.439034328206173	22.25832739126006	35.906420834606635	21.39621744592713
50	19.51376863264405	23.61108281552409	34.24637533530271	22.62877321652915
51	17.633017554582185	24.062340837323013	32.01419306644936	26.290448541645446
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127.0
1	309.5
2	492.0
3	4474.0
4	8456.0
5	5744.0
6	3032.0
7	2947.5
8	2863.0
9	2826.0
10	2789.0
11	2697.5
12	2606.0
13	2446.5
14	2287.0
15	2177.5
16	2068.0
17	1945.0
18	1822.0
19	1826.0
20	1830.0
21	1720.0
22	1610.0
23	1582.0
24	1554.0
25	1773.0
26	2521.0
27	3050.0
28	3536.0
29	4022.0
30	4630.0
31	5238.0
32	5901.5
33	6565.0
34	7363.0
35	8161.0
36	9035.5
37	9910.0
38	11036.5
39	12163.0
40	14091.0
41	16019.0
42	19025.0
43	22031.0
44	25821.5
45	29612.0
46	31032.5
47	32453.0
48	30843.5
49	29234.0
50	24838.0
51	20442.0
52	17802.0
53	15162.0
54	13492.0
55	11822.0
56	10473.0
57	9124.0
58	8149.5
59	7175.0
60	6599.0
61	6023.0
62	5242.0
63	4461.0
64	3779.5
65	3098.0
66	2421.0
67	1744.0
68	1468.0
69	1192.0
70	1016.5
71	841.0
72	698.5
73	556.0
74	489.0
75	345.0
76	268.0
77	200.0
78	132.0
79	84.5
80	37.0
81	21.0
82	5.0
83	8.0
84	11.0
85	8.0
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	294510.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.36134362256805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98017621270162	25.806770886929087
2	11.135478218099365	7.8753094334366684
3	4.402460784722029	4.6703078578030865
4	2.3476902101614616	3.3206992096343377
5	1.4309520250679295	2.530019313291833
6	0.9828671941448455	2.085330275250311
7	0.6685456408827356	1.6548470494237073
8	0.5009099479966213	1.4170279036056972
9	0.39713573589556334	1.2638927899624004
>10	4.898339942899099	40.04161902664216
>50	0.21453312030149232	4.651915768239559
>100	0.03592182479466848	1.9131285873327437
>500	0.003991313866074276	1.0737090171991033
>1k	9.97828466518569E-4	1.6954228812493235
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4805	1.6315235475875185	No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	975	0.33105836813690537	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	914	0.3103459984380836	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	653	0.22172421989066585	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	501	0.17011306916573293	TruSeq Adapter, Index 19 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	381	0.12936742385657535	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	344	0.11680418321958508	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2502461716070762	0.0
2	0.0	0.0	0.0	1.33204305456521	0.0
3	0.0	0.0	0.0	1.7632678007537945	0.0
4	0.0	0.0	0.0	2.392108926691793	0.0
5	3.395470442429799E-4	0.0	0.0	3.9075073851482123	0.0
6	3.395470442429799E-4	0.0	0.0	4.702387015721028	0.0
7	3.395470442429799E-4	0.0	0.0	5.5013412108247595	0.0
8	3.395470442429799E-4	0.0	0.0	6.873111269566398	0.0
9	3.395470442429799E-4	0.0	0.0	7.347798037418085	0.0
10	3.395470442429799E-4	0.0	0.0	8.478150147702964	0.0
11	3.395470442429799E-4	0.0	0.0	10.072323520423755	0.0
12	3.395470442429799E-4	0.0	0.0	11.305558385114258	0.0
13	3.395470442429799E-4	0.0	0.0	11.816576686699943	0.0
14	3.395470442429799E-4	0.0	0.0	12.032189059794234	0.0
15	3.395470442429799E-4	0.0	0.0	12.448134188991885	0.0
16	3.395470442429799E-4	0.0	0.0	13.405656853757089	0.0
17	3.395470442429799E-4	0.0	0.0	14.436861227123018	0.0
18	3.395470442429799E-4	0.0	0.0	15.698957590574175	0.0
19	3.395470442429799E-4	0.0	0.0	16.332212828087332	0.0
20	3.395470442429799E-4	0.0	0.0	16.93966249023802	0.0
21	3.395470442429799E-4	0.0	0.0	17.72469525652779	0.0
22	3.395470442429799E-4	0.0	0.0	18.493090217649655	0.0
23	3.395470442429799E-4	0.0	0.0	19.258429255373333	0.0
24	3.395470442429799E-4	0.0	0.0	19.84958065940036	0.0
25	3.395470442429799E-4	0.0	0.0	20.365352619605446	0.0
26	3.395470442429799E-4	0.0	0.0	20.83087161726257	0.0
27	3.395470442429799E-4	0.0	0.0	21.307595667379715	0.0
28	3.395470442429799E-4	0.0	0.0	21.792129299514446	0.0
29	3.395470442429799E-4	0.0	0.0	22.273946555295236	0.0
30	3.395470442429799E-4	0.0	0.0	22.850497436419815	0.0
31	3.395470442429799E-4	0.0	0.0	23.413126888730435	0.0
32	3.395470442429799E-4	0.0	0.0	23.92074971987369	0.0
33	3.395470442429799E-4	0.0	0.0	24.40188788156599	0.0
34	3.395470442429799E-4	0.0	0.0	24.883026043258294	0.0
35	3.395470442429799E-4	0.0	0.0	25.428678143356763	0.0
36	3.395470442429799E-4	0.0	0.0	25.926114563172728	0.0
37	3.395470442429799E-4	0.0	0.0	26.407592271909273	0.0
38	3.395470442429799E-4	0.0	0.0	26.885334963159146	0.0
39	3.395470442429799E-4	0.0	0.0	27.36783131302842	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	25	3.8837163E-5	45.000004	26
CACGGGT	35	1.207809E-7	45.000004	4
ACGTTGG	25	3.8837163E-5	45.000004	2
ATTCGGG	25	3.8837163E-5	45.000004	3
CACGACC	25	3.8837163E-5	45.000004	27
CGACAAT	35	1.207809E-7	45.000004	20
AGGCGAT	25	3.8837163E-5	45.000004	7
ACGGGAT	25	3.8837163E-5	45.000004	5
TCGTGCA	25	3.8837163E-5	45.000004	21
CTGCCTA	25	3.8837163E-5	45.000004	18
TCTCGCG	25	3.8837163E-5	45.000004	32
GTCAAGC	25	3.8837163E-5	45.000004	16
CGCAGTA	25	3.8837163E-5	45.000004	37
TGCCTAA	25	3.8837163E-5	45.000004	19
GACGGGT	25	3.8837163E-5	45.000004	4
GCTAGTC	25	3.8837163E-5	45.000004	27
AGACACG	25	3.8837163E-5	45.000004	24
CTCGCGC	25	3.8837163E-5	45.000004	33
GCGATAT	30	2.1599844E-6	45.000004	9
CGTACCT	25	3.8837163E-5	45.000004	45
>>END_MODULE