##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936645.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174380
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.070363573804336	31.0	31.0	33.0	28.0	34.0
2	31.145848147723363	31.0	31.0	34.0	28.0	34.0
3	30.769526321825897	31.0	30.0	34.0	27.0	34.0
4	34.71329280880835	35.0	35.0	37.0	32.0	37.0
5	35.06861452001376	35.0	35.0	37.0	33.0	37.0
6	35.07287532973965	35.0	35.0	37.0	32.0	37.0
7	30.789597430898038	35.0	35.0	37.0	0.0	37.0
8	32.9528099552701	35.0	35.0	37.0	17.0	37.0
9	36.273735520128454	39.0	35.0	39.0	32.0	39.0
10	36.1101559811905	37.0	35.0	39.0	32.0	39.0
11	36.36315517834614	37.0	35.0	39.0	32.0	39.0
12	36.26601674503957	38.0	35.0	39.0	32.0	39.0
13	35.91470925564858	38.0	35.0	39.0	31.0	39.0
14	36.98679321023053	39.0	35.0	40.0	31.0	41.0
15	37.23037618993004	39.0	35.0	41.0	32.0	41.0
16	37.34298658102993	39.0	35.0	40.0	32.0	41.0
17	37.208808349581375	39.0	35.0	40.0	32.0	41.0
18	37.07106892992316	39.0	35.0	40.0	32.0	41.0
19	36.76496731276522	38.0	35.0	40.0	31.0	41.0
20	36.63765913522193	38.0	35.0	40.0	32.0	41.0
21	36.657397637343735	38.0	35.0	40.0	31.0	41.0
22	36.71419887601789	38.0	35.0	40.0	32.0	41.0
23	36.81113086363115	38.0	35.0	40.0	32.0	41.0
24	36.66063768780823	38.0	35.0	40.0	32.0	41.0
25	36.39364032572543	38.0	35.0	40.0	31.0	41.0
26	36.35351531138892	38.0	35.0	40.0	31.0	41.0
27	36.388834728753295	38.0	35.0	40.0	31.0	41.0
28	36.31151508200482	38.0	35.0	40.0	31.0	41.0
29	36.31543181557518	38.0	35.0	40.0	31.0	41.0
30	36.07428604197729	38.0	35.0	40.0	30.0	41.0
31	35.74907099438009	38.0	35.0	40.0	30.0	41.0
32	35.46629774056658	38.0	34.0	40.0	29.0	41.0
33	35.171097602936115	38.0	34.0	40.0	26.0	41.0
34	34.88097258859961	38.0	34.0	40.0	24.0	41.0
35	34.757552471613714	38.0	34.0	40.0	23.0	41.0
36	34.43881179034293	38.0	34.0	40.0	22.0	41.0
37	34.307523798600755	38.0	33.0	40.0	21.0	41.0
38	34.05247734831976	37.0	33.0	40.0	20.0	41.0
39	34.06433077187751	37.0	33.0	40.0	20.0	41.0
40	34.01723248078908	37.0	33.0	40.0	19.0	41.0
41	33.785049891042554	37.0	33.0	40.0	18.0	41.0
42	33.85697327675192	37.0	33.0	40.0	18.0	41.0
43	33.724303245785066	37.0	33.0	40.0	18.0	41.0
44	33.42071338456245	37.0	33.0	40.0	17.0	41.0
45	33.32604656497305	37.0	33.0	40.0	17.0	41.0
46	33.12636196811561	36.0	32.0	40.0	15.0	41.0
47	33.0331402683794	36.0	32.0	40.0	15.0	41.0
48	32.927583438467714	36.0	32.0	40.0	15.0	41.0
49	32.88401192797339	36.0	32.0	40.0	14.0	41.0
50	32.67886798944833	36.0	31.0	40.0	13.0	41.0
51	30.81466911343044	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	10.0
10	10.0
11	8.0
12	3.0
13	2.0
14	6.0
15	12.0
16	23.0
17	46.0
18	133.0
19	205.0
20	390.0
21	635.0
22	945.0
23	1439.0
24	2198.0
25	3220.0
26	4134.0
27	4273.0
28	4298.0
29	4269.0
30	4901.0
31	5722.0
32	7472.0
33	10099.0
34	16061.0
35	16953.0
36	15085.0
37	21952.0
38	28441.0
39	21427.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.446381465764425	20.319990824635852	23.436747333409794	18.796880376189932
2	35.122720495469665	24.86638375960546	23.89780938180984	16.113086363115038
3	27.921206560385365	25.441564399587108	33.300837251978436	13.336391788049088
4	26.15609588255534	22.052414267691248	35.528730358986124	16.26275949076729
5	25.77130404862943	25.93474022250258	30.758114462667734	17.535841266200254
6	22.21470352104599	34.03142562220438	30.41461176740452	13.33925908934511
7	61.28971212294988	17.56967542149329	17.344878999885307	3.7957334556715217
8	72.16997362082807	6.798944833123064	16.144053217112052	4.887028328936804
9	68.80949650189243	5.7443514164468406	16.95320564284895	8.49294643881179
10	40.95825209312995	20.295905493749284	25.262071338456245	13.483771074664524
11	33.24865236839087	23.00894598004358	28.937951599954125	14.804450051611424
12	29.227549030852163	20.51554077302443	33.235462782429174	17.021447413694233
13	19.58080055052185	28.91329280880835	35.56141759376075	15.94448904690905
14	16.74045188668425	31.370570019497652	33.65580915242574	18.23316894139236
15	14.04289482738846	24.314714990251176	46.01101043697672	15.631379745383644
16	16.599380662920062	21.793210230531024	41.257024888175245	20.350384218373666
17	17.502007110907215	20.561991054019956	36.51565546507627	25.42034636999656
18	19.07099438008946	22.36667048973506	38.609932331689414	19.952402798486066
19	21.266200252322516	24.549260236265628	34.365179493061135	19.81936001835073
20	23.604771189356576	23.892648239477005	35.085445578621396	17.417134992545016
21	19.94896203693084	27.94586535153114	34.518866842527814	17.586305769010206
22	18.5652024314715	21.879802729670832	35.22192912031196	24.333065718545704
23	17.208395458194747	26.819589402454408	34.107695836678516	21.864319302672325
24	19.53435026952632	21.838513591008144	37.53240050464503	21.09473563482051
25	15.5539626103911	27.35233398325496	36.27938983828421	20.814313568069736
26	17.074779217800206	27.428604197729094	34.10310815460489	21.39350842986581
27	17.343732079366898	29.762013992430326	34.10310815460489	18.79114577359789
28	15.649157013418971	24.295790801697443	41.97901135451313	18.076040830370456
29	18.48434453492373	23.27445808005505	38.73838742975112	19.502809955270102
30	20.17203807776121	25.69446037389609	36.81098749856635	17.32251404977635
31	24.185686431930268	23.320334900791376	32.89941507053561	19.594563596742745
32	22.817983713728637	26.689413923615096	33.61738731505907	16.8752150475972
33	21.705470810872807	23.776809267117788	33.58125931872921	20.936460603280192
34	22.381580456474367	24.454065833237756	33.03131093015254	20.133042780135337
35	20.963413235462784	23.453377680926714	34.28030737469893	21.302901708911573
36	24.67484803303131	23.019841724968458	33.172382153916736	19.132928088083496
37	20.357839201743317	25.184080743204497	36.62633329510265	17.831746759949535
38	18.21596513361624	26.12398210803991	34.273425851588485	21.386626906755364
39	21.330427801353366	24.561876361968118	34.50797109760293	19.599724739075583
40	22.145888289941507	22.78013533662117	35.48400045876821	19.589975914669115
41	19.339373781396947	23.28707420575754	32.533547425163434	24.840004587682074
42	21.61084986810414	21.919371487555914	33.947126964101386	22.52265168023856
43	22.59204037160225	23.968344993691936	29.995985778185574	23.44362885652024
44	20.128455098061703	25.330313109301528	33.354169056084416	21.187062736552356
45	19.98164927170547	25.934166762243375	31.316091294873267	22.768092671177886
46	23.371372863860536	26.098176396375734	31.02133272164239	19.509118018121345
47	20.229384103681614	22.736552356921667	35.74664525748366	21.287418281913066
48	21.556944603738962	23.26815001720381	33.58584700080284	21.589058378254386
49	19.581947471040255	20.078564055510952	36.84195435256336	23.497534120885422
50	20.29074435141645	23.62312191765111	33.71258171808694	22.37355201284551
51	19.290629659364605	24.731620598692512	30.80800550521849	25.169744236724394
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100.0
1	164.5
2	229.0
3	2326.5
4	4424.0
5	3071.0
6	1718.0
7	1696.0
8	1674.0
9	1673.0
10	1672.0
11	1575.0
12	1478.0
13	1437.5
14	1397.0
15	1348.5
16	1300.0
17	1206.0
18	1112.0
19	1085.5
20	1059.0
21	1220.5
22	1382.0
23	1213.0
24	1044.0
25	966.0
26	1012.0
27	1136.0
28	1497.5
29	1859.0
30	2332.5
31	2806.0
32	3058.5
33	3311.0
34	3514.0
35	3717.0
36	4622.0
37	5527.0
38	6250.5
39	6974.0
40	8056.0
41	9138.0
42	11346.5
43	13555.0
44	14573.0
45	15591.0
46	19150.5
47	22710.0
48	19701.5
49	16693.0
50	15598.5
51	14504.0
52	12526.0
53	10548.0
54	9026.0
55	7504.0
56	6367.5
57	5231.0
58	4813.5
59	4396.0
60	4097.5
61	3799.0
62	3134.5
63	2470.0
64	2061.5
65	1653.0
66	1175.0
67	697.0
68	580.5
69	464.0
70	374.0
71	284.0
72	227.5
73	171.0
74	119.5
75	54.0
76	40.0
77	29.5
78	19.0
79	19.0
80	19.0
81	14.0
82	9.0
83	8.5
84	8.0
85	4.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	174380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.78598463126505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.40910771050491	30.411744466108498
2	8.500036963110816	6.59364606032802
3	3.2217047386708066	3.748709714416791
4	1.752051452650255	2.718201628627136
5	1.1621202040363716	2.253698818671866
6	0.8176240112367857	1.902741140038995
7	0.6002809196421971	1.6297740566578738
8	0.39328749907592225	1.220323431586191
9	0.3134471797146448	1.0941621745613028
>10	4.716492940045834	40.14049776350499
>50	0.08279736822650995	1.9669686890698475
>100	0.023656390921859984	1.804679435715105
>500	0.001478524432616249	0.4398440188094965
>1k	0.005914097730464996	4.075008601903888
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2510	1.4393852506021332	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	1784	1.0230531024200022	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	1690	0.9691478380548229	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	1122	0.6434224108269296	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	767	0.4398440188094965	RNA PCR Primer, Index 35 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	415	0.23798600756967542	RNA PCR Primer, Index 10 (95% over 23bp)
ACTGATGGGGTTTTATTATTAGTAATATTAAATACTTATTTTGCTTCTAAT	316	0.18121344190847574	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT	301	0.17261153802041518	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	267	0.15311388920747793	TruSeq Adapter, Index 10 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	228	0.13074893909852048	RNA PCR Primer, Index 35 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTC	215	0.123293955728868	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTA	210	0.1204266544328478	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	175	0.10035554536070651	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.734602592040372E-4	0.0	0.0	0.21332721642390182	0.0
2	5.734602592040372E-4	0.0	0.0	1.312077073058837	0.0
3	5.734602592040372E-4	0.0	0.0	1.5833237756623466	0.0
4	5.734602592040372E-4	0.0	0.0	2.06675077417135	0.0
5	5.734602592040372E-4	0.0	0.0	3.67760064227549	0.0
6	5.734602592040372E-4	0.0	0.0	4.220094047482509	0.0
7	5.734602592040372E-4	0.0	0.0	4.9707535267805945	0.0
8	5.734602592040372E-4	0.0	0.0	6.120541346484688	0.0
9	5.734602592040372E-4	0.0	0.0	6.477807087968804	0.0
10	5.734602592040372E-4	0.0	0.0	8.598463126505333	0.0
11	5.734602592040372E-4	0.0	0.0	9.869824521160684	0.0
12	5.734602592040372E-4	0.0	0.0	12.180295905493749	0.0
13	5.734602592040372E-4	0.0	0.0	12.733685055625646	0.0
14	5.734602592040372E-4	0.0	0.0	12.955040715678404	0.0
15	5.734602592040372E-4	0.0	0.0	13.447643078334671	0.0
16	5.734602592040372E-4	0.0	0.0	14.23156325266659	0.0
17	5.734602592040372E-4	0.0	0.0	15.0711090721413	0.0
18	5.734602592040372E-4	0.0	0.0	16.152655121000116	0.0
19	5.734602592040372E-4	0.0	0.0	16.78002064456933	0.0
20	5.734602592040372E-4	0.0	0.0	17.309897924073862	0.0
21	5.734602592040372E-4	0.0	0.0	17.908590434682875	0.0
22	5.734602592040372E-4	0.0	0.0	18.542837481362543	0.0
23	5.734602592040372E-4	0.0	0.0	19.246473219405896	0.0
24	5.734602592040372E-4	0.0	0.0	19.751691707764653	0.0
25	5.734602592040372E-4	0.0	0.0	20.17433191879803	0.0
26	5.734602592040372E-4	0.0	0.0	20.560844133501547	0.0
27	5.734602592040372E-4	0.0	0.0	20.932446381465766	0.0
28	5.734602592040372E-4	0.0	0.0	21.325840119279732	0.0
29	5.734602592040372E-4	0.0	0.0	21.746759949535498	0.0
30	5.734602592040372E-4	0.0	0.0	22.243949994265396	0.0
31	5.734602592040372E-4	0.0	0.0	22.70329166188783	0.0
32	5.734602592040372E-4	0.0	0.0	23.138547998623697	0.0
33	5.734602592040372E-4	0.0	0.0	23.573804335359558	0.0
34	5.734602592040372E-4	0.0	0.0	23.99300378483771	0.0
35	5.734602592040372E-4	0.0	0.0	24.440876247276062	0.0
36	5.734602592040372E-4	0.0	0.0	24.853194173643768	0.0
37	5.734602592040372E-4	0.0	0.0	25.25863057690102	0.0
38	5.734602592040372E-4	0.0	0.0	25.6818442481936	0.0
39	5.734602592040372E-4	0.0	0.0	26.06950338341553	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGGA	20	7.0168154E-4	45.0	4
ACTATAC	20	7.0168154E-4	45.0	41
ATCTCAG	20	7.0168154E-4	45.0	33
GCGACCG	20	7.0168154E-4	45.0	19
CTGTACT	20	7.0168154E-4	45.0	24
CCAGGGT	20	7.0168154E-4	45.0	4
CACGCCG	20	7.0168154E-4	45.0	26
ACACAAT	20	7.0168154E-4	45.0	35
TTGTACG	20	7.0168154E-4	45.0	1
TACGGGT	35	1.2040073E-7	45.0	4
TACGGGA	40	6.7575456E-9	45.0	4
TGAAACG	35	1.2040073E-7	45.0	4
AGGCGAT	20	7.0168154E-4	45.0	7
TAACGAG	20	7.0168154E-4	45.0	23
TCGATGG	20	7.0168154E-4	45.0	2
ATCGTGA	20	7.0168154E-4	45.0	30
ATCCGTA	20	7.0168154E-4	45.0	44
AGGCCAC	20	7.0168154E-4	45.0	19
CTAGTAG	20	7.0168154E-4	45.0	13
CGAGGAC	20	7.0168154E-4	45.0	26
>>END_MODULE