##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936643.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1031091
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20017340855463	31.0	31.0	33.0	28.0	34.0
2	31.228792608993775	31.0	31.0	34.0	28.0	34.0
3	30.96642100454761	31.0	31.0	34.0	28.0	34.0
4	34.94747408327684	35.0	35.0	37.0	32.0	37.0
5	35.20463470246564	35.0	35.0	37.0	33.0	37.0
6	35.185234862878254	36.0	35.0	37.0	32.0	37.0
7	31.07209256990896	35.0	35.0	37.0	0.0	37.0
8	33.1451074638417	37.0	35.0	37.0	17.0	37.0
9	36.514149575546675	39.0	35.0	39.0	32.0	39.0
10	36.13687928611539	37.0	35.0	39.0	32.0	39.0
11	36.42893692215333	38.0	35.0	39.0	32.0	39.0
12	36.58190887128294	38.0	35.0	39.0	32.0	39.0
13	36.62277723304732	39.0	35.0	39.0	32.0	39.0
14	37.72190427421052	39.0	37.0	41.0	33.0	41.0
15	37.88816796965544	40.0	37.0	41.0	33.0	41.0
16	37.797907265217134	39.0	37.0	41.0	33.0	41.0
17	37.693910624765415	39.0	36.0	41.0	33.0	41.0
18	37.601844066139655	39.0	36.0	40.0	33.0	41.0
19	37.394513190397355	39.0	36.0	40.0	32.0	41.0
20	37.393536554969444	39.0	36.0	40.0	32.0	41.0
21	37.385783602029306	39.0	36.0	40.0	32.0	41.0
22	37.492965218394886	39.0	36.0	40.0	33.0	41.0
23	37.538985404779986	39.0	36.0	41.0	33.0	41.0
24	37.47311633987689	39.0	36.0	41.0	33.0	41.0
25	37.154893215050855	39.0	35.0	40.0	32.0	41.0
26	37.15817808515446	39.0	35.0	40.0	32.0	41.0
27	37.21898164177556	39.0	35.0	40.0	32.0	41.0
28	37.23225205146782	39.0	35.0	41.0	32.0	41.0
29	37.23856090296589	39.0	35.0	41.0	32.0	41.0
30	37.01234905551498	39.0	35.0	40.0	31.0	41.0
31	37.00909619034596	39.0	35.0	40.0	31.0	41.0
32	36.86899604399612	39.0	35.0	40.0	31.0	41.0
33	36.73610088731257	39.0	35.0	40.0	31.0	41.0
34	36.64906977172723	39.0	35.0	40.0	30.0	41.0
35	36.5584773797851	39.0	35.0	41.0	30.0	41.0
36	36.508801841932474	39.0	35.0	40.0	30.0	41.0
37	36.442779541282	39.0	35.0	40.0	30.0	41.0
38	36.33708275991159	39.0	35.0	40.0	30.0	41.0
39	36.310802829236216	39.0	35.0	40.0	30.0	41.0
40	36.23011935900905	39.0	35.0	40.0	30.0	41.0
41	36.07209644929497	39.0	35.0	40.0	29.0	41.0
42	36.071749244247115	39.0	35.0	40.0	29.0	41.0
43	36.026779401624104	39.0	35.0	40.0	29.0	41.0
44	35.85410502079836	39.0	35.0	40.0	28.0	41.0
45	35.77985260272857	38.0	35.0	40.0	28.0	41.0
46	35.66992535091471	38.0	35.0	40.0	28.0	41.0
47	35.639965822609255	38.0	35.0	40.0	28.0	41.0
48	35.56660372362866	38.0	35.0	40.0	28.0	41.0
49	35.46904007502732	38.0	35.0	40.0	27.0	41.0
50	35.320320902810714	38.0	34.0	40.0	27.0	41.0
51	33.732028501849015	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	33.0
9	85.0
10	94.0
11	68.0
12	35.0
13	33.0
14	36.0
15	58.0
16	112.0
17	214.0
18	445.0
19	762.0
20	1249.0
21	1845.0
22	3013.0
23	4408.0
24	6582.0
25	9177.0
26	11978.0
27	13494.0
28	14712.0
29	16925.0
30	20578.0
31	25990.0
32	34319.0
33	47124.0
34	71201.0
35	82037.0
36	102854.0
37	158363.0
38	219076.0
39	184182.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.00665314700642	21.775575579653008	25.30513795581573	12.912633317524836
2	33.965867222194746	24.116494082481566	25.747290976257187	16.170347719066502
3	31.815329587786138	24.06276458624893	28.719773521444765	15.402132304520164
4	28.64315564775563	25.675522335080025	27.587574714549927	18.093747302614418
5	25.987424970249958	29.302263330782637	26.598913190009416	18.111398508957986
6	25.976271735472427	34.61954376480834	25.75068543901557	13.653499060703664
7	70.08149620159617	17.24454970511817	9.307616883475852	3.3663372098098034
8	82.68872485551711	5.016628018283546	8.87312565040331	3.421521475796026
9	76.5361156289794	7.461029142917551	10.487629122938714	5.515226105164336
10	37.27255887210731	29.434744363009667	18.845184372669337	14.447512392213685
11	27.939435025618497	27.548295931202972	27.13950563044387	17.372763412734667
12	25.276721453295586	25.577179899737267	29.930917833634467	19.215180813332676
13	23.49288278144218	25.505702212510826	31.955375422731848	19.04603958331515
14	20.41856635350323	28.2785903475057	30.884761868739037	20.418081430252034
15	20.18056602181573	27.214377780428688	33.28930230212464	19.315753895630937
16	23.254882449754678	27.032725530530282	30.759845639230676	18.95254638048436
17	23.376210247204174	26.36983544614394	29.709113938536944	20.54484036811494
18	23.963549289054022	25.924675901545065	30.820751999581024	19.291022809819893
19	23.057615671167724	27.71530349891523	28.916846330731232	20.310234499185814
20	25.009431757235784	27.109634358170133	29.949732855780915	17.93120102881317
21	23.633801478240038	27.872903555554263	30.036340148444708	18.45695481776099
22	23.663090842612338	24.679974900372518	30.434753091628185	21.222181165386957
23	21.683052223324616	26.655067302498033	31.052642298303446	20.609238175873905
24	21.32032963142923	26.518027991709754	31.49169180993724	20.669950566923774
25	20.904071512601703	28.72695038556248	29.56809825708885	20.800879844746973
26	21.18047776578401	29.3511435945033	29.085114698896607	20.383263940816086
27	21.27542573836839	28.635784814337434	30.04710544462128	20.041684002672895
28	19.99716804821301	28.485749560417073	30.815708797768576	20.701373593601343
29	21.33933862287616	27.276253987281436	30.63376559391945	20.75064179592296
30	20.78148291469909	27.146294555960626	31.737838852244852	20.334383677095428
31	23.196012767059358	27.279551465389574	28.770787447470692	20.75364832008038
32	22.27727717534146	27.739161722874123	29.412631862755084	20.57092923902934
33	22.091260616182275	26.486701949682423	29.851584389738633	21.570453044396665
34	21.93055705073558	27.13281368957735	30.151072989677925	20.785556270009145
35	21.08873028665753	27.287601191359446	30.760330562481876	20.86333795950115
36	22.76724362835094	28.031376474045455	28.93100608966619	20.270373807937418
37	21.97255140428924	27.969112328591756	29.595544913106604	20.4627913540124
38	21.54998928319615	28.002959971525303	29.231270566807392	21.215780178471153
39	22.530504097116548	26.28274323022895	29.548701327040973	21.63805134561353
40	22.769765229257168	26.17819377727087	30.46627310295599	20.585767890515967
41	21.359123491524993	27.92537225133378	29.498172324266235	21.217331932874984
42	22.29948666024628	26.872991811585976	29.743543489371937	21.0839780387958
43	22.522745325097397	26.36770178383867	28.96650247165381	22.14305041941012
44	21.768010776934336	26.49232705939631	29.246206202944258	22.493455960725093
45	21.578308801066054	26.5496449876878	29.02731184735392	22.84473436389223
46	22.233343128783005	26.933704202635848	29.214007299064775	21.618945369516368
47	21.222278150037194	26.8599958684539	30.600596843537573	21.31712913797133
48	21.533016969404255	26.51773703775903	30.378017071238133	21.57122892159858
49	22.212588413631774	25.67503741182883	30.59487474917345	21.517499425365948
50	20.755006105183732	26.327356169339076	30.789716911504417	22.127920813972775
51	21.035291744375616	25.635952597782346	29.56809825708885	23.760657400753182
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	261.0
1	816.0
2	1371.0
3	6194.0
4	11017.0
5	7708.5
6	4400.0
7	4062.0
8	3724.0
9	3724.5
10	3725.0
11	3577.0
12	3429.0
13	3469.0
14	3509.0
15	3405.5
16	3302.0
17	3305.0
18	3308.0
19	3443.0
20	3578.0
21	3921.0
22	4264.0
23	4877.0
24	5490.0
25	6689.5
26	9455.5
27	11022.0
28	12804.0
29	14586.0
30	18462.5
31	22339.0
32	25479.0
33	28619.0
34	33007.0
35	37395.0
36	40248.0
37	43101.0
38	46144.0
39	49187.0
40	54308.0
41	59429.0
42	62279.0
43	65129.0
44	69344.5
45	73560.0
46	83679.5
47	93799.0
48	92225.0
49	90651.0
50	87333.5
51	84016.0
52	76320.0
53	68624.0
54	62014.0
55	55404.0
56	50020.0
57	44636.0
58	41058.0
59	37480.0
60	33364.0
61	29248.0
62	26013.0
63	22778.0
64	18946.0
65	15114.0
66	12603.0
67	10092.0
68	8203.5
69	6315.0
70	5036.0
71	3757.0
72	3072.0
73	2387.0
74	1883.0
75	1194.0
76	1009.0
77	707.5
78	406.0
79	273.5
80	141.0
81	124.0
82	107.0
83	77.5
84	48.0
85	43.0
86	38.0
87	25.0
88	12.0
89	11.0
90	10.0
91	7.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1031091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.14253513789935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7327387476526	23.378146108720774
2	9.231686093946193	5.934595893134446
3	3.970230244924032	3.8283979545906432
4	2.441216330195788	3.138675266901273
5	1.6737112473146025	2.6898661288753485
6	1.205109942286574	2.3241173198986846
7	0.8798744902910924	1.9796977704785907
8	0.6973921172985998	1.7932760508131422
9	0.5473419253711983	1.5833661361820301
>10	6.366029356394767	45.84954885512931
>50	0.2250914966026512	4.350222869329609
>100	0.02662020694997291	1.4638115011821338
>500	0.001478900385969247	0.28416786236114433
>1k	0.0011831203087753976	0.8442323913572972
>5k	2.957800771938494E-4	0.5578778910455653
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5868	0.5691059276048379	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	2673	0.2592399700899339	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCG	2485	0.24100685584492543	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGC	2390	0.2317933140721818	No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	1332	0.1291835541188896	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9396930047881322E-4	0.0	0.0	0.06973196352213336	0.0
2	1.9396930047881322E-4	0.0	0.0	0.3592311444867621	0.0
3	1.9396930047881322E-4	0.0	0.0	0.49433076227025546	0.0
4	1.9396930047881322E-4	0.0	0.0	0.7709309847530431	0.0
5	1.9396930047881322E-4	0.0	0.0	1.5253745789653872	0.0
6	1.9396930047881322E-4	0.0	0.0	1.9970109330796215	0.0
7	1.9396930047881322E-4	0.0	0.0	2.3867922423917967	0.0
8	1.9396930047881322E-4	0.0	0.0	3.0069120960225626	0.0
9	1.9396930047881322E-4	0.0	0.0	3.2478219672172486	0.0
10	1.9396930047881322E-4	0.0	0.0	3.9912093113023004	0.0
11	1.9396930047881322E-4	0.0	0.0	4.658463704949417	0.0
12	1.9396930047881322E-4	0.0	0.0	5.4482097118489055	0.0
13	1.9396930047881322E-4	0.0	0.0	5.71879688601685	0.0
14	1.9396930047881322E-4	0.0	0.0	5.850986964293162	0.0
15	1.9396930047881322E-4	0.0	0.0	6.0686205194303895	0.0
16	1.9396930047881322E-4	0.0	0.0	6.455492289235383	0.0
17	1.9396930047881322E-4	0.0	0.0	6.904725189144314	0.0
18	1.9396930047881322E-4	0.0	0.0	7.381986652972434	0.0
19	1.9396930047881322E-4	0.0	0.0	7.704751568969179	0.0
20	1.9396930047881322E-4	0.0	0.0	8.023540114306108	0.0
21	1.9396930047881322E-4	0.0	0.0	8.41225459246565	0.0
22	1.9396930047881322E-4	0.0	0.0	8.826088095037198	0.0
23	1.9396930047881322E-4	0.0	0.0	9.248747200780532	0.0
24	1.9396930047881322E-4	0.0	0.0	9.581986459003133	0.0
25	1.9396930047881322E-4	0.0	0.0	9.904945344300357	0.0
26	1.9396930047881322E-4	0.0	0.0	10.216072102268374	0.0
27	1.9396930047881322E-4	0.0	0.0	10.510517500395212	0.0
28	1.9396930047881322E-4	0.0	0.0	10.821353304412511	0.0
29	1.9396930047881322E-4	0.0	0.0	11.14993729942362	0.0
30	1.9396930047881322E-4	0.0	0.0	11.546022611001357	0.0
31	1.9396930047881322E-4	0.0	0.0	11.898852768572318	0.0
32	1.9396930047881322E-4	0.0	0.0	12.257792959108363	0.0
33	1.9396930047881322E-4	0.0	0.0	12.600536713054424	0.0
34	1.9396930047881322E-4	0.0	0.0	12.935036771730138	0.0
35	1.9396930047881322E-4	0.0	0.0	13.28932169905469	0.0
36	1.9396930047881322E-4	0.0	0.0	13.643121703128045	0.0
37	1.9396930047881322E-4	0.0	0.0	13.983537825468364	0.0
38	1.9396930047881322E-4	0.0	0.0	14.347230263866138	0.0
39	1.9396930047881322E-4	0.0	0.0	14.741666836389804	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAACG	20	7.032947E-4	45.000004	23
TTACGCG	20	7.032947E-4	45.000004	1
ATCGACG	20	7.032947E-4	45.000004	1
ACGCATT	50	2.1827873E-11	45.0	39
CTAACGC	50	2.1827873E-11	45.0	36
CGTTTTT	3510	0.0	41.474358	1
CCCGCAC	230	0.0	41.08696	33
CGTTTTA	165	0.0	39.545452	1
TCAAGCG	185	0.0	38.918922	17
CTATGCG	35	6.247663E-6	38.571426	1
TCAATCG	35	6.247663E-6	38.571426	8
TATCGCG	35	6.247663E-6	38.571426	1
CACGTGA	170	0.0	38.38235	43
TTAAGCG	65	9.094947E-12	38.076927	1
TATGCGG	95	0.0	37.894737	2
GTTACGG	95	0.0	37.894737	2
TACGGTT	60	1.546141E-10	37.500004	33
CCGCACT	255	0.0	37.058823	34
TACGGCT	250	0.0	36.899998	7
TAGTGCG	55	2.748493E-9	36.81818	1
>>END_MODULE