##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936642.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	719570
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.201052017176924	31.0	31.0	33.0	28.0	34.0
2	31.222021485053574	31.0	31.0	34.0	28.0	34.0
3	31.024561891129423	31.0	31.0	34.0	28.0	34.0
4	34.9884667231819	35.0	35.0	37.0	32.0	37.0
5	35.2106744305627	35.0	35.0	37.0	33.0	37.0
6	35.183099628945065	36.0	35.0	37.0	32.0	37.0
7	31.062880609252748	35.0	35.0	37.0	0.0	37.0
8	33.078695609878125	36.0	35.0	37.0	17.0	37.0
9	36.31353586169518	39.0	35.0	39.0	32.0	39.0
10	36.116224967689035	37.0	35.0	39.0	32.0	39.0
11	36.40925969676334	38.0	35.0	39.0	32.0	39.0
12	36.48753422182693	38.0	35.0	39.0	32.0	39.0
13	36.42626012757619	39.0	35.0	39.0	32.0	39.0
14	37.56294314660144	39.0	36.0	41.0	33.0	41.0
15	37.69722334171797	39.0	36.0	41.0	33.0	41.0
16	37.66221493391887	39.0	36.0	41.0	33.0	41.0
17	37.52570840918882	39.0	36.0	41.0	32.0	41.0
18	37.433271259224256	39.0	36.0	40.0	32.0	41.0
19	37.25554011423489	39.0	36.0	40.0	32.0	41.0
20	37.24236002056784	39.0	35.0	40.0	32.0	41.0
21	37.291715885876286	39.0	35.0	40.0	32.0	41.0
22	37.37672776797254	39.0	35.0	40.0	32.0	41.0
23	37.42362938977445	39.0	35.0	41.0	32.0	41.0
24	37.36498047445002	39.0	35.0	41.0	32.0	41.0
25	37.08484511583307	39.0	35.0	40.0	32.0	41.0
26	37.044155537334795	39.0	35.0	40.0	32.0	41.0
27	37.06884806203705	39.0	35.0	40.0	32.0	41.0
28	37.05954389427019	39.0	35.0	40.0	32.0	41.0
29	37.04093972789305	39.0	35.0	41.0	32.0	41.0
30	36.87409286101422	39.0	35.0	40.0	31.0	41.0
31	36.910635518434624	39.0	35.0	40.0	31.0	41.0
32	36.78079964423197	39.0	35.0	40.0	31.0	41.0
33	36.63672887974763	39.0	35.0	40.0	31.0	41.0
34	36.610749475381134	39.0	35.0	40.0	30.0	41.0
35	36.548855566518895	39.0	35.0	40.0	30.0	41.0
36	36.4862431730061	39.0	35.0	40.0	30.0	41.0
37	36.45182261628472	39.0	35.0	40.0	30.0	41.0
38	36.356820045304836	39.0	35.0	40.0	30.0	41.0
39	36.31098572758731	39.0	35.0	40.0	30.0	41.0
40	36.194513389941214	39.0	35.0	40.0	30.0	41.0
41	36.0303709159637	38.0	35.0	40.0	29.0	41.0
42	36.07293939436052	38.0	35.0	40.0	30.0	41.0
43	36.02942729685785	38.0	35.0	40.0	30.0	41.0
44	35.88934502550134	38.0	35.0	40.0	29.0	41.0
45	35.83157580221521	38.0	35.0	40.0	29.0	41.0
46	35.7432341537307	38.0	35.0	40.0	29.0	41.0
47	35.704376224689746	38.0	35.0	40.0	29.0	41.0
48	35.624132468001726	38.0	35.0	40.0	28.0	41.0
49	35.50055032866851	38.0	34.0	40.0	28.0	41.0
50	35.35543032644496	38.0	34.0	40.0	27.0	41.0
51	33.71421126506108	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	21.0
9	74.0
10	95.0
11	67.0
12	29.0
13	31.0
14	35.0
15	50.0
16	84.0
17	163.0
18	302.0
19	608.0
20	1059.0
21	1599.0
22	2356.0
23	3109.0
24	4180.0
25	5581.0
26	7200.0
27	8500.0
28	9743.0
29	11679.0
30	14808.0
31	18938.0
32	25415.0
33	36056.0
34	55452.0
35	61211.0
36	71732.0
37	107861.0
38	148211.0
39	123310.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.59621718526342	20.35451728115402	23.234987562016205	11.814277971566352
2	32.90534624845394	25.843628833886907	24.312714537848994	16.938310379810165
3	32.99512208680184	25.268007282126824	25.93312672846283	15.803743902608502
4	29.307086176466502	24.65375154606223	27.381491724224187	18.657670553247076
5	27.19554734077296	30.04349820031408	23.561710465972734	19.19924399294023
6	26.093917200550326	36.2139889100435	24.173186764317578	13.518907125088594
7	67.90944591909057	18.353044179162556	10.224578567755744	3.512931333991134
8	78.91004349820031	7.788401406395486	9.14212654779938	4.159428547604819
9	71.83164945731478	8.77871506594216	11.363731117194991	8.025904359548065
10	40.34228775518713	26.543352279833787	18.704782022596827	14.409577942382256
11	32.34501160415248	27.99143933182317	22.988868351932403	16.674680712091945
12	30.195950359242325	23.824089386717066	27.442639354058674	18.537320899981935
13	25.674222104868182	24.124407632335977	29.920091165557206	20.281279097238627
14	20.56575454785497	29.40297677779785	27.511013521964507	22.520255152382674
15	19.87492530261128	27.766026932751505	33.607293244576624	18.751754520060594
16	22.43881762719402	26.730547410258904	30.900815764970744	19.929819197576332
17	22.135858915741345	25.81916978195311	29.176035687980324	22.868935614325224
18	23.408702419500536	27.03600761565935	28.35665744819823	21.198632516641883
19	25.757188320802705	28.34025876565171	25.638923245827367	20.26362966771822
20	27.804800088942006	26.499854079519714	27.766721792181443	17.928624039356837
21	25.906722070125216	28.349708853898857	26.4456550439846	19.29791403199133
22	23.113665105549146	25.803049043178568	27.976847283794488	23.1064385674778
23	23.814917242241897	27.736286949150184	27.776032908542604	20.672762900065315
24	24.070208596800867	25.509957335631	27.825923815612104	22.59391025195603
25	23.719443556568507	27.30422335561516	26.24956571285629	22.726767374960048
26	21.712550551023526	28.082326945258973	27.87998387926123	22.325138624456272
27	22.39587531442389	28.86376586016649	27.521158469641595	21.219200355768027
28	20.70333671498256	28.53426351849021	29.0032936336979	21.759106132829327
29	21.638756479564183	28.884194727406648	28.571230040162877	20.905818752866296
30	22.375446447183737	27.95099851300082	28.606389927317704	21.06716511249774
31	23.00165376544325	27.68681295773865	25.35333602012313	23.958197256694973
32	24.19611712550551	30.2826688160985	25.695067887766303	19.826146170629684
33	23.04848729102103	29.512208680184	25.574023375071224	21.86528065372375
34	22.34667926678433	27.65109718303987	27.853162305265645	22.149061244910154
35	22.273996970412885	28.082882832802923	27.037953222063177	22.605166974721016
36	21.10607724057423	29.96859235376683	27.618021874174854	21.307308531484082
37	21.980627319093347	30.94778826243451	26.644523812832666	20.42706060563948
38	22.4179718442959	29.9035535111247	24.639854357463488	23.038620287115915
39	22.848506747085064	26.570590769487335	27.3049182150451	23.27598426838251
40	23.622024264491294	26.735133482496494	28.568033686785167	21.074808566227052
41	20.198173909418124	27.61941159303473	28.766207596203287	23.41620690134386
42	22.11098294814959	27.778534402490372	28.421001431410424	21.68948121794961
43	22.68938393762942	27.190822296649387	27.355920897202495	22.763872868518696
44	21.508817766165905	27.72808760787693	27.216254151785098	23.546840474172075
45	21.604847339383245	26.251372347374126	26.274580652334034	25.8691996609086
46	21.973122837250024	28.342065400169545	26.362132940506132	23.322678822074295
47	20.803535444779524	27.069082924524366	30.155509540419974	21.971872090276136
48	21.86653140069764	26.347123976819493	28.08802479258446	23.698319829898413
49	20.554636796975974	26.051669747210138	29.85505232291508	23.538641132898814
50	19.799741512292062	26.465666995566796	29.249829759439667	24.484761732701475
51	19.575996775852246	26.465389051794823	27.132315132648664	26.826299039704267
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	266.0
1	652.5
2	1039.0
3	3170.5
4	5302.0
5	3684.5
6	2067.0
7	1931.0
8	1795.0
9	1781.0
10	1767.0
11	1775.5
12	1784.0
13	1745.0
14	1706.0
15	1592.0
16	1478.0
17	1677.0
18	1876.0
19	1939.0
20	2002.0
21	2699.0
22	3396.0
23	3482.5
24	3569.0
25	4389.0
26	6114.5
27	7020.0
28	9535.5
29	12051.0
30	12893.5
31	13736.0
32	15504.5
33	17273.0
34	19899.5
35	22526.0
36	23741.0
37	24956.0
38	27190.5
39	29425.0
40	33765.0
41	38105.0
42	41930.0
43	45755.0
44	58558.0
45	71361.0
46	65374.5
47	59388.0
48	57695.0
49	56002.0
50	49745.0
51	43488.0
52	40577.0
53	37666.0
54	35056.5
55	32447.0
56	32351.5
57	32256.0
58	32921.0
59	33586.0
60	34654.0
61	35722.0
62	31965.5
63	28209.0
64	23534.5
65	18860.0
66	14289.5
67	9719.0
68	8111.5
69	6504.0
70	5177.0
71	3850.0
72	3282.0
73	2714.0
74	2327.5
75	1350.5
76	760.0
77	615.0
78	470.0
79	354.5
80	239.0
81	173.5
82	108.0
83	86.5
84	65.0
85	67.5
86	70.0
87	46.5
88	23.0
89	16.5
90	10.0
91	7.0
92	4.0
93	3.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	719570.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.679882968541637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.62962002912425	21.853185054825968
2	9.009998585587491	5.3483140713392485
3	3.7696601312849554	3.356492145831443
4	2.1241096498366288	2.521733032780044
5	1.463722102856445	2.172155035562348
6	1.0785542078813166	1.9206817599087365
7	0.7994784627985678	1.6609899048211745
8	0.6511856441617352	1.5461690967611759
9	0.5060222146867118	1.351681210024547
>10	6.543380445095119	45.47692386511593
>50	0.3671107733690852	6.550999188791239
>100	0.05065199277750659	2.6623002512309766
>500	0.0037175774515601167	0.7526380599371828
>1k	0.0027881830886700874	2.825737323070002
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	4826	0.6706783217755048	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGC	4497	0.6249565712856289	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCG	4339	0.6029990132996095	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2732	0.37967119251775366	No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	2224	0.3090734744361216	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	1870	0.25987742679655906	No Hit
GAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	940	0.13063357282821686	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	891	0.12382395041483107	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTC	780	0.1083980710702225	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10172742054282419	0.0
2	0.0	0.0	0.0	0.6396875912003002	0.0
3	0.0	0.0	0.0	0.8070097419292077	0.0
4	0.0	0.0	0.0	1.1730616896201898	0.0
5	0.0	0.0	0.0	2.4263101574551467	0.0
6	0.0	0.0	0.0	3.0694720458051337	0.0
7	0.0	0.0	0.0	3.510985727587309	0.0
8	0.0	0.0	0.0	4.198479647567297	0.0
9	0.0	0.0	0.0	4.427922231332602	0.0
10	0.0	0.0	0.0	5.609322234112039	0.0
11	0.0	0.0	0.0	6.331837069360868	0.0
12	0.0	0.0	0.0	7.595230484872911	0.0
13	0.0	0.0	0.0	7.928207123698876	0.0
14	0.0	0.0	0.0	8.097474880831609	0.0
15	0.0	0.0	0.0	8.408216017899578	0.0
16	0.0	0.0	0.0	8.884194727406646	0.0
17	0.0	0.0	0.0	9.390747251830955	0.0
18	0.0	0.0	0.0	9.960254040607586	0.0
19	0.0	0.0	0.0	10.309490390094084	0.0
20	0.0	0.0	0.0	10.656920105062746	0.0
21	0.0	0.0	0.0	11.098294814958933	0.0
22	0.0	0.0	0.0	11.538279805995247	0.0
23	0.0	0.0	0.0	11.977014050057674	0.0
24	0.0	0.0	0.0	12.330141612351822	0.0
25	0.0	0.0	0.0	12.628514251566909	0.0
26	0.0	0.0	0.0	12.921328015342496	0.0
27	0.0	0.0	0.0	13.210111594424449	0.0
28	0.0	0.0	0.0	13.519324040746557	0.0
29	0.0	0.0	0.0	13.842433675667413	0.0
30	0.0	0.0	0.0	14.201120113401059	0.0
31	0.0	0.0	0.0	14.555359450783106	0.0
32	0.0	0.0	0.0	14.911822338340954	0.0
33	0.0	0.0	0.0	15.25133065580833	0.0
34	0.0	0.0	0.0	15.60487513376044	0.0
35	0.0	0.0	0.0	15.968425587503647	0.0
36	0.0	0.0	0.0	16.33114220993093	0.0
37	0.0	0.0	0.0	16.69191322595439	0.0
38	0.0	0.0	0.0	17.039620884695026	0.0
39	0.0	0.0	0.0	17.40942507330767	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGACG	35	1.2110831E-7	45.000004	1
TCCGAAC	20	7.031526E-4	45.0	35
CGACCCT	20	7.031526E-4	45.0	12
CCCGTCG	20	7.031526E-4	45.0	15
TCGCTCG	20	7.031526E-4	45.0	13
GTCGTAG	25	3.889347E-5	45.0	1
GTCGTAC	20	7.031526E-4	45.0	22
TTACGCG	20	7.031526E-4	45.0	1
GACGATA	20	7.031526E-4	45.0	9
GCTCGAC	20	7.031526E-4	45.0	16
TCGCGCC	20	7.031526E-4	45.0	17
TCGACGG	75	0.0	42.0	2
ATAGCGG	55	6.002665E-11	40.909092	2
ACGGGTA	75	0.0	39.0	5
TACGGCT	630	0.0	38.928574	7
CGTTTTT	1410	0.0	38.617023	1
ATGGGCG	205	0.0	38.41463	5
ACGGCTG	690	0.0	38.152176	8
CGTATGG	65	9.094947E-12	38.076927	2
AGGACGT	30	1.13949754E-4	37.500004	5
>>END_MODULE