##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936641.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336303
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04707659461854	31.0	31.0	33.0	28.0	34.0
2	31.101048756627208	31.0	31.0	34.0	28.0	34.0
3	30.75879192276013	31.0	30.0	34.0	27.0	34.0
4	34.70528362815675	35.0	35.0	37.0	32.0	37.0
5	35.064016675438516	35.0	35.0	37.0	33.0	37.0
6	35.07777212811066	35.0	35.0	37.0	32.0	37.0
7	30.909804551252886	35.0	35.0	37.0	0.0	37.0
8	33.08529510590152	36.0	35.0	37.0	17.0	37.0
9	36.45035578035284	39.0	35.0	39.0	32.0	39.0
10	36.07777212811066	37.0	35.0	39.0	32.0	39.0
11	36.37616673059711	37.0	35.0	39.0	32.0	39.0
12	36.46395958406556	38.0	35.0	39.0	32.0	39.0
13	36.40550931749048	39.0	35.0	39.0	32.0	39.0
14	37.41470340734397	39.0	36.0	41.0	32.0	41.0
15	37.645260375316305	39.0	36.0	41.0	33.0	41.0
16	37.56950428631323	39.0	36.0	41.0	33.0	41.0
17	37.492198999116866	39.0	36.0	40.0	32.0	41.0
18	37.35906310678168	39.0	36.0	40.0	32.0	41.0
19	37.06994287889195	38.0	36.0	40.0	32.0	41.0
20	36.956473775137304	39.0	35.0	40.0	32.0	41.0
21	36.92548683776238	38.0	35.0	40.0	32.0	41.0
22	37.0064554880569	39.0	35.0	40.0	32.0	41.0
23	37.03660984290952	39.0	35.0	40.0	32.0	41.0
24	36.93509127185901	38.0	35.0	40.0	32.0	41.0
25	36.63284894871589	38.0	35.0	40.0	31.0	41.0
26	36.62392247467314	38.0	35.0	40.0	31.0	41.0
27	36.60478199718706	38.0	35.0	40.0	31.0	41.0
28	36.62845112889269	38.0	35.0	40.0	31.0	41.0
29	36.622236495065465	38.0	35.0	40.0	31.0	41.0
30	36.30785333464168	38.0	35.0	40.0	30.0	41.0
31	36.18195496323256	38.0	35.0	40.0	30.0	41.0
32	35.909260399104376	38.0	35.0	40.0	30.0	41.0
33	35.63239400183763	38.0	35.0	40.0	27.0	41.0
34	35.42615736404373	38.0	35.0	40.0	26.0	41.0
35	35.231823682809846	38.0	34.0	40.0	25.0	41.0
36	35.061221576970766	38.0	34.0	40.0	24.0	41.0
37	34.92839492957244	38.0	34.0	40.0	23.0	41.0
38	34.82813415283242	38.0	34.0	40.0	23.0	41.0
39	34.80913937728774	38.0	34.0	40.0	23.0	41.0
40	34.70577425714311	38.0	34.0	40.0	23.0	41.0
41	34.573432291713125	38.0	34.0	40.0	22.0	41.0
42	34.55870450159529	38.0	34.0	40.0	22.0	41.0
43	34.453216296018766	38.0	34.0	40.0	22.0	41.0
44	34.2013690035474	38.0	33.0	40.0	21.0	41.0
45	34.11912174437933	38.0	33.0	40.0	20.0	41.0
46	33.98156127063987	37.0	33.0	40.0	20.0	41.0
47	33.933024683098274	37.0	33.0	40.0	20.0	41.0
48	33.87578463468955	37.0	33.0	40.0	20.0	41.0
49	33.774530705940776	37.0	33.0	40.0	20.0	41.0
50	33.59922450885065	37.0	33.0	40.0	19.0	41.0
51	31.910794729752634	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	13.0
10	11.0
11	15.0
12	9.0
13	10.0
14	12.0
15	23.0
16	37.0
17	93.0
18	184.0
19	377.0
20	559.0
21	938.0
22	1480.0
23	2256.0
24	3315.0
25	5038.0
26	6671.0
27	7449.0
28	7654.0
29	7738.0
30	8820.0
31	10309.0
32	12822.0
33	17200.0
34	26465.0
35	29927.0
36	31974.0
37	46898.0
38	62079.0
39	45917.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.280107522085736	22.221032818618923	25.772294627166577	18.726565032128764
2	34.99760632524836	22.907913399523643	25.7047959726794	16.389684302548595
3	28.912320139873867	21.118158327460655	34.86587987618309	15.103641656482397
4	26.699732086838356	23.692325075898818	32.116870798060084	17.49107203920274
5	24.630467168000287	27.39612789656946	31.269420730710102	16.70398420472015
6	24.26442820908526	31.476971659485642	30.253670053493426	14.00493007793567
7	64.65122226087784	17.7860441328207	13.860417540134936	3.702316066166522
8	78.13073329705652	3.6095425851092613	14.09889296259623	4.160831155237984
9	72.98477860738679	5.694567101691034	16.350433983639753	4.970220307282421
10	37.00859046752482	23.293874868793914	24.86507702875086	14.832457634930405
11	27.970609836962502	26.426763959881416	29.575115297811795	16.02751090534429
12	25.864770757323008	22.846659113953784	33.39250616259742	17.896063966125787
13	21.34146885397989	24.66287841619016	36.148354311439384	17.847298418390558
14	17.661751456276036	28.27688126481179	35.19623672699916	18.865130551913005
15	16.89607288665281	24.517473825686956	41.13671302367211	17.44974026398813
16	18.817851758681904	23.908499180798266	39.025521627817774	18.24812743270206
17	18.549938597038977	23.77885418803876	34.2896138303851	23.381593384537158
18	19.987927553426523	24.763680371569684	36.669907791485656	18.578484283518137
19	22.162454691156487	25.16569878948448	34.20457147274928	18.467275046609753
20	23.54840723989973	25.65900393395242	33.2057697968796	17.58681902926825
21	20.85678688563587	25.71996086862145	34.43561312268996	18.987639123052723
22	19.6376481922552	23.596875436734134	34.147777450691784	22.61769892031888
23	19.012021896920338	24.95606640440317	34.999093079752484	21.032818618924008
24	19.501758830578378	23.84129787721192	36.33063041364484	20.326312878564863
25	18.7185365578065	24.833557833263455	34.706797144241946	21.741108464688093
26	18.83539546183055	26.890928716068547	34.91702423112491	19.356651590975996
27	19.688495196296195	25.822844280306747	35.65772532507887	18.830935198318183
28	16.427745217854138	26.187396484717652	38.07340404337755	19.311454254050663
29	20.298361893887357	23.946560096103813	36.5239084991808	19.231169510828032
30	20.389648620440497	24.324790441952644	36.17868410332349	19.10687683428337
31	21.270104637782	25.4686993574247	32.63336931279233	20.627826692000966
32	22.241847381676642	25.8356303690422	32.00506685935005	19.917455389931106
33	21.09496495719634	24.224880539275595	33.49955248689426	21.18060201663381
34	19.59661376794141	23.938234270880724	33.69907494134753	22.76607701983033
35	20.568059160935228	23.175231859364917	33.26851083695358	22.988198142746274
36	21.0679060252213	24.41667187030743	33.657445815232094	20.85797628923917
37	19.553200536421027	25.56979866370505	33.88164839445381	20.99535240542011
38	19.017671564036004	27.292649783082517	31.142154545157197	22.547524107724286
39	21.085747079270778	23.538892011073347	31.960464224226364	23.41489668542951
40	20.292117524970042	24.296839457275134	32.61374415333791	22.79729886441691
41	17.86424741973756	24.400614921662907	31.483513379303783	26.251624279295754
42	20.413139341605635	23.921285269533723	31.225710148288893	24.439865240571745
43	21.13837818871672	24.235882522606104	32.7710427798741	21.854696508803073
44	19.631701174238707	24.6899373481652	32.91020300145999	22.7681584761361
45	20.97424049146157	24.272456683407523	31.045515502389215	23.707787322741694
46	21.238288091393773	24.15024546316863	32.27179061738967	22.339675828047923
47	19.370329732413925	23.70154295382438	33.235504886962055	23.69262242679964
48	18.44675783445286	23.145199418381637	33.89830004489999	24.509742702265516
49	19.677195862064863	21.854101807001424	34.594101152829445	23.874601178104268
50	18.394126725006913	22.556741985649843	34.081170848907085	24.967960440436155
51	18.396802883114336	22.82941276170596	31.913185430995263	26.860598924184444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	185.0
1	331.0
2	477.0
3	5468.5
4	10460.0
5	7099.0
6	3738.0
7	3359.0
8	2980.0
9	2831.0
10	2682.0
11	2561.5
12	2441.0
13	2289.5
14	2138.0
15	1923.0
16	1708.0
17	1595.5
18	1483.0
19	1505.0
20	1527.0
21	1524.5
22	1522.0
23	1746.0
24	1970.0
25	2455.0
26	3586.5
27	4233.0
28	4782.0
29	5331.0
30	5785.5
31	6240.0
32	6759.0
33	7278.0
34	8234.0
35	9190.0
36	10101.5
37	11013.0
38	11872.5
39	12732.0
40	14288.0
41	15844.0
42	17662.0
43	19480.0
44	20782.5
45	22085.0
46	24119.0
47	26153.0
48	27593.5
49	29034.0
50	30409.0
51	31784.0
52	30355.0
53	28926.0
54	24641.5
55	20357.0
56	17383.5
57	14410.0
58	12580.0
59	10750.0
60	9969.0
61	9188.0
62	8212.5
63	7237.0
64	5683.0
65	4129.0
66	3070.5
67	2012.0
68	1563.0
69	1114.0
70	934.5
71	755.0
72	548.5
73	342.0
74	264.5
75	153.5
76	120.0
77	92.0
78	64.0
79	41.0
80	18.0
81	16.5
82	15.0
83	11.0
84	7.0
85	10.0
86	13.0
87	9.0
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	336303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.50040528806333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.7512862981401	29.16455223570512
2	10.078073664030196	7.760198411762397
3	3.8211961257637905	4.413527985812501
4	2.0668287921223016	3.18294984630988
5	1.2332946138623058	2.3741171236642167
6	0.8379170610621456	1.935608786920531
7	0.571199730813774	1.5393994795734083
8	0.4779923336854763	1.4722318857182433
9	0.3871518761281353	1.3414953725070569
>10	4.5695565401618845	38.301241085490986
>50	0.17471600329660109	4.135251914495205
>100	0.027708264839989602	1.8564925291517347
>500	0.002309022069999133	0.6892580403522642
>1k	0.0	0.0
>5k	7.696740233330444E-4	1.833675302536462
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6188	1.8400073743023406	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	938	0.2789151449734317	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	874	0.2598846873206602	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	514	0.15283836302382078	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	427	0.12696883465208458	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	347	0.10318076258612026	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19268338373431101	0.0
2	0.0	0.0	0.0	1.0945486659351835	0.0
3	0.0	0.0	0.0	1.3904128122556147	0.0
4	0.0	0.0	0.0	1.7644802454929038	0.0
5	0.0	0.0	0.0	2.822454750626667	0.0
6	0.0	0.0	0.0	3.4076413234493894	0.0
7	0.0	0.0	0.0	4.069841779585671	0.0
8	0.0	0.0	0.0	5.278573191437483	0.0
9	0.0	0.0	0.0	5.734709473302349	0.0
10	0.0	0.0	0.0	6.867616405443901	0.0
11	0.0	0.0	0.0	8.64161187976319	0.0
12	0.0	0.0	0.0	9.890188312325492	0.0
13	0.0	0.0	0.0	10.421851722999795	0.0
14	0.0	0.0	0.0	10.645162249519036	0.0
15	0.0	0.0	0.0	11.010903857533236	0.0
16	0.0	0.0	0.0	11.871140013618671	0.0
17	2.9735090082455405E-4	0.0	0.0	12.910976113802137	0.0
18	2.9735090082455405E-4	0.0	0.0	14.147361159430632	0.0
19	2.9735090082455405E-4	0.0	0.0	14.77982652548446	0.0
20	2.9735090082455405E-4	0.0	0.0	15.439945525314968	0.0
21	2.9735090082455405E-4	0.0	0.0	16.251713484566	0.0
22	2.9735090082455405E-4	0.0	0.0	17.07359137444507	0.0
23	2.9735090082455405E-4	0.0	0.0	17.913607669274434	0.0
24	2.9735090082455405E-4	0.0	0.0	18.518122050650753	0.0
25	2.9735090082455405E-4	0.0	0.0	19.027484143763214	0.0
26	2.9735090082455405E-4	0.0	0.0	19.497001216165184	0.0
27	2.9735090082455405E-4	0.0	0.0	19.98198053541003	0.0
28	2.9735090082455405E-4	0.0	0.0	20.544271088869262	0.0
29	2.9735090082455405E-4	0.0	0.0	21.050362322072655	0.0
30	2.9735090082455405E-4	0.0	0.0	21.677772722812463	0.0
31	2.9735090082455405E-4	0.0	0.0	22.23173745104861	0.0
32	2.9735090082455405E-4	0.0	0.0	22.732179017136332	0.0
33	2.9735090082455405E-4	0.0	0.0	23.227268267009215	0.0
34	2.9735090082455405E-4	0.0	0.0	23.7223575168821	0.0
35	5.947018016491081E-4	0.0	0.0	24.2867295266471	0.0
36	5.947018016491081E-4	0.0	0.0	24.8062015503876	0.0
37	5.947018016491081E-4	0.0	0.0	25.31496894169841	0.0
38	5.947018016491081E-4	0.0	0.0	25.824033683910045	0.0
39	5.947018016491081E-4	0.0	0.0	26.31942028468375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGCGG	45	3.8380676E-10	45.000004	2
AGCGTTG	20	7.026163E-4	45.0	1
TTTAGCG	20	7.026163E-4	45.0	1
ATCCTAC	25	3.8849008E-5	45.0	13
ACTATGC	20	7.026163E-4	45.0	17
GATACGC	20	7.026163E-4	45.0	35
GCGACGT	20	7.026163E-4	45.0	17
AATCACG	25	3.8849008E-5	45.0	45
TTGTGCG	20	7.026163E-4	45.0	1
CGACGTT	20	7.026163E-4	45.0	27
CGACGTC	20	7.026163E-4	45.0	18
CGCGGGT	50	2.1827873E-11	45.0	4
GCGAACG	20	7.026163E-4	45.0	1
AACCCCG	25	3.8849008E-5	45.0	10
TTTCGCG	25	3.8849008E-5	45.0	1
TTTCGAC	20	7.026163E-4	45.0	24
GCCGGTC	20	7.026163E-4	45.0	21
TTACTAG	20	7.026163E-4	45.0	1
AGGCGAT	55	1.8189894E-12	45.0	7
ATGTCGG	20	7.026163E-4	45.0	2
>>END_MODULE