##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936637.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723354
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15295139032894	31.0	31.0	33.0	28.0	34.0
2	31.193165448729115	31.0	31.0	34.0	28.0	34.0
3	31.01384107919497	31.0	31.0	34.0	28.0	34.0
4	34.960461959151395	35.0	35.0	37.0	32.0	37.0
5	35.17990361565706	35.0	35.0	37.0	33.0	37.0
6	35.14560920379233	36.0	35.0	37.0	32.0	37.0
7	31.065353893114576	35.0	35.0	37.0	0.0	37.0
8	33.08520033068179	36.0	35.0	37.0	17.0	37.0
9	36.408067142782095	39.0	35.0	39.0	32.0	39.0
10	36.11458013642007	37.0	35.0	39.0	32.0	39.0
11	36.35082407783741	38.0	35.0	39.0	32.0	39.0
12	36.45385661792152	38.0	35.0	39.0	32.0	39.0
13	36.420343289730894	39.0	35.0	39.0	32.0	39.0
14	37.465001921604085	39.0	36.0	41.0	32.0	41.0
15	37.62790694459421	39.0	36.0	41.0	33.0	41.0
16	37.56492671637953	39.0	36.0	41.0	33.0	41.0
17	37.481378412229695	39.0	36.0	41.0	32.0	41.0
18	37.42627123096022	39.0	36.0	40.0	32.0	41.0
19	37.22851881651307	39.0	36.0	40.0	32.0	41.0
20	37.23566746019238	39.0	35.0	40.0	32.0	41.0
21	37.259549266334325	39.0	35.0	40.0	32.0	41.0
22	37.37017698111851	39.0	36.0	40.0	32.0	41.0
23	37.42000182483265	39.0	36.0	40.0	32.0	41.0
24	37.36622594193161	39.0	35.0	41.0	32.0	41.0
25	37.04157853554415	39.0	35.0	40.0	31.0	41.0
26	37.035210975538945	39.0	35.0	40.0	31.0	41.0
27	37.077006555573064	39.0	35.0	40.0	32.0	41.0
28	37.088361991500705	39.0	35.0	40.0	32.0	41.0
29	37.07433013434639	39.0	35.0	41.0	31.0	41.0
30	36.82869936434996	39.0	35.0	40.0	31.0	41.0
31	36.83094307904567	39.0	35.0	40.0	31.0	41.0
32	36.708140136088275	39.0	35.0	40.0	31.0	41.0
33	36.624037469897175	39.0	35.0	40.0	30.0	41.0
34	36.59407565313802	39.0	35.0	40.0	30.0	41.0
35	36.49901569632573	39.0	35.0	40.0	30.0	41.0
36	36.42993472075913	39.0	35.0	40.0	30.0	41.0
37	36.34426850477083	39.0	35.0	40.0	30.0	41.0
38	36.23913602468501	39.0	35.0	40.0	30.0	41.0
39	36.21051518343715	39.0	35.0	40.0	30.0	41.0
40	36.08697677762202	39.0	35.0	40.0	29.0	41.0
41	35.9281154179005	38.0	35.0	40.0	29.0	41.0
42	35.91743461707546	38.0	35.0	40.0	29.0	41.0
43	35.89837894032521	38.0	35.0	40.0	29.0	41.0
44	35.710491681804484	38.0	35.0	40.0	28.0	41.0
45	35.59606223232332	38.0	34.0	40.0	28.0	41.0
46	35.520587430220885	38.0	34.0	40.0	28.0	41.0
47	35.44131365831944	38.0	34.0	40.0	28.0	41.0
48	35.38323835908836	38.0	34.0	40.0	27.0	41.0
49	35.27832430594149	38.0	34.0	40.0	27.0	41.0
50	35.12639592785828	38.0	34.0	40.0	27.0	41.0
51	33.471741360385096	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	23.0
9	69.0
10	55.0
11	38.0
12	28.0
13	32.0
14	24.0
15	44.0
16	87.0
17	178.0
18	335.0
19	607.0
20	959.0
21	1511.0
22	2317.0
23	3126.0
24	4321.0
25	6029.0
26	7474.0
27	8978.0
28	10513.0
29	12609.0
30	15750.0
31	20216.0
32	26819.0
33	37090.0
34	54160.0
35	60256.0
36	73725.0
37	110278.0
38	146565.0
39	119131.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.467375586503984	21.353030466410637	24.41128410156023	10.76830984552515
2	32.35082684273537	25.033386142884396	26.325146470469505	16.29064054391073
3	33.14753219032451	23.562045692703713	27.20770189976139	16.082720217210383
4	28.86138737049909	25.513095939194365	26.57993181761627	19.045584872690274
5	27.111621695601322	29.121702513568735	24.94546238771058	18.82121340311936
6	26.112387572336644	36.06463778454256	24.245113734077645	13.577860909043151
7	70.52259336369191	17.52945307553425	8.520724292669978	3.4272292681038605
8	82.57547480210243	6.020288821241052	7.7946620879956425	3.6095742886608773
9	76.20238500098154	7.45167649587892	9.893496130525303	6.4524423726142395
10	40.56520044127772	28.720239329567544	16.729153360595227	13.985406868559517
11	29.340405942318696	26.73739275652032	25.837833204765577	18.084368096395405
12	26.678362185043564	24.14571565236385	29.300591411673953	19.87533075091864
13	25.15835953074152	23.668632509117252	31.31440484188931	19.85860311825192
14	21.217135731605826	26.53320504206792	30.377657412553187	21.87200181377306
15	19.959383648946435	27.031854389413756	32.80703500637309	20.201726955266714
16	24.38335863214968	25.670003898506128	29.18114781973971	20.765489649604483
17	24.350456346408535	25.724057653652295	28.465868717114994	21.45961728282418
18	24.933435081578313	25.59300149028	29.251514472858382	20.222048955283306
19	24.354603693350697	27.04954973636698	27.607782634781863	20.988063935500463
20	24.82104197944575	26.97005891997556	28.41651528850328	19.792383812075414
21	25.073200673529144	27.45294834894118	29.00806520735352	18.46578577017615
22	24.339534999460845	23.79208520309558	29.76827390185165	22.100105895591923
23	22.55410214086049	26.474312715489233	29.275569085122914	21.69601605852736
24	22.46714609997318	25.859261163966746	29.90181847338924	21.77177426267084
25	22.292542793708197	27.66266585931646	28.07159426781355	21.973197079161793
26	22.39857663052945	27.164431246664844	27.535480553090185	22.90151156971552
27	21.49583744611905	27.811002634947755	28.727981043859575	21.965178875073615
28	20.168409934831356	27.561747083723876	30.183699820558125	22.08614316088665
29	22.243327609994555	26.493114021627033	28.875764839898583	22.387793528479833
30	23.013904671848085	27.38700553256082	28.756044758168198	20.843045037422893
31	24.499069611835974	27.206181205882597	27.010841164906807	21.28390801737462
32	24.180691611576073	27.367789491728807	27.04954973636698	21.40196916032814
33	23.455873610984387	26.35431614396271	27.168855083403148	23.02095516164976
34	23.328273570063896	26.18233949076109	28.68885773770519	21.80052920146982
35	22.17379042626432	28.135186920926685	27.581654349046246	22.10936830376275
36	24.374925693367285	28.881432880719537	26.385006511334698	20.358634914578477
37	21.775230385122637	28.47001606405716	27.896576226854346	21.858177323965858
38	21.74979332387738	29.139121370725814	27.095723532323042	22.015361773073767
39	23.64499263154693	26.02902589879921	27.28041871614728	23.045562753506584
40	24.16949377483224	26.207223572414062	28.16283590054109	21.460446752212608
41	21.529845691044773	27.17867047116626	28.383198268067915	22.90828556972105
42	21.479662793044625	27.340969981502834	28.780099370432733	22.39926785501981
43	22.1209808752008	26.87356398112128	27.942335288116194	23.06311985556173
44	22.242221650809977	26.264595205113956	28.46752765589186	23.025655488184206
45	22.200333446694152	26.039117776358463	27.652712226655275	24.10783655029211
46	23.673747570345917	26.750387776939093	27.53368336941525	22.04218128329974
47	21.293170425545448	26.541499735952247	30.26249941245918	21.902830426043128
48	21.297179527589535	27.18212659361806	28.777334472471296	22.743359406321108
49	21.907392507679504	26.381135654188682	29.42805320769637	22.283418630435445
50	21.866057283155964	25.48793536774525	29.8861967999071	22.75981054919168
51	21.422982384835088	25.92962781708541	27.86533287989007	24.782056918189436
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	241.0
1	682.5
2	1124.0
3	2808.5
4	4493.0
5	3056.5
6	1620.0
7	1535.5
8	1451.0
9	1464.0
10	1477.0
11	1489.5
12	1502.0
13	1507.0
14	1512.0
15	1542.0
16	1572.0
17	1616.0
18	1660.0
19	1821.5
20	1983.0
21	2280.0
22	2577.0
23	2957.0
24	3337.0
25	3897.5
26	5906.0
27	7354.0
28	9011.5
29	10669.0
30	12208.5
31	13748.0
32	16386.0
33	19024.0
34	21643.0
35	24262.0
36	26389.0
37	28516.0
38	29940.5
39	31365.0
40	34058.0
41	36751.0
42	39785.0
43	42819.0
44	46093.5
45	49368.0
46	58288.0
47	67208.0
48	63274.5
49	59341.0
50	58293.5
51	57246.0
52	51849.0
53	46452.0
54	42637.0
55	38822.0
56	37253.0
57	35684.0
58	33547.0
59	31410.0
60	28947.0
61	26484.0
62	24660.0
63	22836.0
64	19396.0
65	15956.0
66	13404.0
67	10852.0
68	9227.5
69	7603.0
70	6052.5
71	4502.0
72	3658.5
73	2815.0
74	2217.5
75	1250.5
76	881.0
77	607.5
78	334.0
79	293.5
80	253.0
81	176.0
82	99.0
83	64.0
84	29.0
85	23.5
86	18.0
87	16.0
88	14.0
89	11.0
90	8.0
91	6.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	723354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.6642913445131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.45105436005578	22.523245304584197
2	8.90539838085928	5.4615546097924845
3	3.7480460902310324	3.447935318505228
4	2.183367861357824	2.678057128516912
5	1.5112768718320844	2.317111715004171
6	1.1244253210575141	2.068782338403318
7	0.8552821984378586	1.8358635760271917
8	0.6433550967611966	1.57824225000502
9	0.5076045660868351	1.4008800872062679
>10	6.782461414417503	48.24915660018379
>50	0.2531105832950755	4.655357552915699
>100	0.03102065762279769	1.8406921741451612
>500	0.0013487242444694648	0.21643848962012457
>1k	0.002247873740782441	1.726682855090484
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC	3142	0.43436546974233914	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG	2766	0.3823853880672534	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC	2715	0.3753348982655795	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2479	0.3427091023205789	No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	1423	0.19672248995650812	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.764897961440733E-4	0.0	0.0	0.07119612250709888	0.0
2	2.764897961440733E-4	0.0	0.0	0.46229093915289055	0.0
3	2.764897961440733E-4	0.0	0.0	0.5979091841615585	0.0
4	2.764897961440733E-4	0.0	0.0	0.856012408862051	0.0
5	2.764897961440733E-4	0.0	0.0	1.7452035932613907	0.0
6	2.764897961440733E-4	0.0	0.0	2.1636708997254455	0.0
7	2.764897961440733E-4	0.0	0.0	2.5243518387953894	0.0
8	2.764897961440733E-4	0.0	0.0	3.1353942882737913	0.0
9	2.764897961440733E-4	0.0	0.0	3.337093594560893	0.0
10	2.764897961440733E-4	0.0	0.0	4.244256615709597	0.0
11	4.1473469421610994E-4	0.0	0.0	4.91985943258764	0.0
12	4.1473469421610994E-4	0.0	0.0	5.94618955587444	0.0
13	4.1473469421610994E-4	0.0	0.0	6.213278698949615	0.0
14	4.1473469421610994E-4	0.0	0.0	6.340878739870105	0.0
15	4.1473469421610994E-4	0.0	0.0	6.582945556394241	0.0
16	4.1473469421610994E-4	0.0	0.0	7.016343311850076	0.0
17	4.1473469421610994E-4	0.0	0.0	7.483058087741272	0.0
18	4.1473469421610994E-4	0.0	0.0	7.98253690447554	0.0
19	4.1473469421610994E-4	0.0	0.0	8.337273312928387	0.0
20	4.1473469421610994E-4	0.0	0.0	8.675420333612587	0.0
21	4.1473469421610994E-4	0.0	0.0	9.100246905387957	0.0
22	4.1473469421610994E-4	0.0	0.0	9.553137191471949	0.0
23	4.1473469421610994E-4	0.0	0.0	9.986534946927783	0.0
24	4.1473469421610994E-4	0.0	0.0	10.337538743132685	0.0
25	4.1473469421610994E-4	0.0	0.0	10.655502008698368	0.0
26	4.1473469421610994E-4	0.0	0.0	10.948304702814943	0.0
27	4.1473469421610994E-4	0.0	0.0	11.286589968397216	0.0
28	4.1473469421610994E-4	0.0	0.0	11.613124417643366	0.0
29	4.1473469421610994E-4	0.0	0.0	11.964404703644412	0.0
30	4.1473469421610994E-4	0.0	0.0	12.369876989689695	0.0
31	4.1473469421610994E-4	8.294693884322199E-4	0.0	12.714521520583283	0.0
32	4.1473469421610994E-4	8.294693884322199E-4	0.0	13.052115561675196	0.0
33	4.1473469421610994E-4	8.294693884322199E-4	0.0	13.415008419114292	0.0
34	4.1473469421610994E-4	8.294693884322199E-4	0.0	13.771818501038219	0.0
35	4.1473469421610994E-4	8.294693884322199E-4	0.0	14.151162501347887	0.0
36	4.1473469421610994E-4	8.294693884322199E-4	0.0	14.514331848583128	0.0
37	4.1473469421610994E-4	8.294693884322199E-4	0.0	14.871971399895488	0.0
38	4.1473469421610994E-4	8.294693884322199E-4	0.0	15.272466869610177	0.0
39	4.1473469421610994E-4	8.294693884322199E-4	0.0	15.765724665931204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGCA	20	7.03155E-4	45.000004	34
CGTTGAT	45	3.8380676E-10	45.000004	25
CGTTAGA	20	7.03155E-4	45.000004	15
GCCGATA	30	2.164381E-6	45.000004	9
CTAACCG	20	7.03155E-4	45.000004	2
TCTATCG	20	7.03155E-4	45.000004	29
CCGCATG	75	0.0	42.0	24
GCGTAAG	60	3.6379788E-12	41.250004	1
TACGGAA	85	0.0	39.705883	5
CGTTTTT	1290	0.0	39.59302	1
ACGGGTA	105	0.0	38.57143	5
GCCGATT	35	6.245078E-6	38.57143	9
TCGCGCA	35	6.245078E-6	38.57143	33
ACATACG	170	0.0	38.38235	17
ATAGCGG	95	0.0	37.894737	2
CGTAAGT	30	1.13950344E-4	37.500004	36
TATAGCG	30	1.13950344E-4	37.500004	1
ACACGAC	85	0.0	37.058823	26
TGTAGCG	55	2.746674E-9	36.81818	1
TACGGGA	130	0.0	36.346153	4
>>END_MODULE