FastQCFastQC Report
Sat 14 Jan 2017
SRR2936636.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936636.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214409
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198931.638080745449021No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC18670.1537373323155543No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT16660.1371860715788503No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15300.1259872085928217No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCG12750.10498934049401809No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT12630.10400120552466262No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGC12220.1006250777126981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG351.2120108E-745.0000041
CGTATAG253.890937E-545.02
CGTTTTT100800.043.7946431
CGTTGAT850.042.35294325
CGTGCGG1550.040.645162
TCGTTGA900.039.99999624
CAACGTT451.9284926E-839.9999968
TCGTAAG451.9284926E-839.9999961
CGTTTTA6650.039.924811
GCTACGA1950.039.2307710
CGAATAT1900.039.07894514
TCGACGT356.2485797E-638.5714326
ACACGAC2450.037.6530626
TACCGCG301.13996015E-437.49999625
TACGGGA2950.037.3728834
TACGAAT2050.037.31707412
AAACACG2500.036.89999840
ACGACCA2500.036.89999828
ACAACGA4350.036.7241413
GTTTTTT123200.036.6720772
Produced by FastQC (version 0.11.5)