##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936636.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1214409
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.116607337396214	31.0	31.0	33.0	28.0	34.0
2	31.203922237071694	31.0	31.0	34.0	28.0	34.0
3	30.852757184770535	31.0	31.0	34.0	27.0	34.0
4	34.791038274584594	35.0	35.0	37.0	32.0	37.0
5	35.127573165218635	35.0	35.0	37.0	33.0	37.0
6	35.13619217248884	36.0	35.0	37.0	32.0	37.0
7	31.11217390516704	35.0	35.0	37.0	0.0	37.0
8	33.1314030116707	36.0	35.0	37.0	17.0	37.0
9	36.46817340780577	39.0	35.0	39.0	32.0	39.0
10	36.22517702026253	37.0	35.0	39.0	32.0	39.0
11	36.49257869465723	38.0	35.0	39.0	32.0	39.0
12	36.62130468400679	39.0	35.0	39.0	32.0	39.0
13	36.680264227290806	39.0	35.0	39.0	32.0	39.0
14	37.79650513130255	40.0	37.0	41.0	33.0	41.0
15	37.939161353382595	40.0	37.0	41.0	33.0	41.0
16	37.82939108652851	40.0	37.0	41.0	33.0	41.0
17	37.694418437281016	39.0	36.0	41.0	33.0	41.0
18	37.53181012327807	39.0	36.0	40.0	33.0	41.0
19	37.254163959588574	39.0	36.0	40.0	32.0	41.0
20	37.171608576682154	39.0	35.0	40.0	32.0	41.0
21	37.13205271041305	39.0	35.0	40.0	32.0	41.0
22	37.19265255774619	39.0	35.0	40.0	32.0	41.0
23	37.22900439637717	39.0	35.0	40.0	32.0	41.0
24	37.14233672510662	39.0	35.0	40.0	32.0	41.0
25	36.805166134308955	39.0	35.0	40.0	31.0	41.0
26	36.813860075147666	39.0	35.0	40.0	31.0	41.0
27	36.85379637337997	39.0	35.0	40.0	31.0	41.0
28	36.835037454432566	39.0	35.0	40.0	31.0	41.0
29	36.79679992490174	39.0	35.0	40.0	31.0	41.0
30	36.50803641936119	39.0	35.0	40.0	31.0	41.0
31	36.40908705386735	39.0	35.0	40.0	30.0	41.0
32	36.11752630291772	38.0	35.0	40.0	30.0	41.0
33	35.834889234187166	38.0	35.0	40.0	28.0	41.0
34	35.570027066663705	38.0	35.0	40.0	26.0	41.0
35	35.3899954628136	38.0	35.0	40.0	25.0	41.0
36	35.2851246985159	38.0	35.0	40.0	24.0	41.0
37	35.19720621306331	38.0	35.0	40.0	24.0	41.0
38	35.074731000840735	38.0	34.0	40.0	23.0	41.0
39	35.00946797989804	38.0	34.0	40.0	23.0	41.0
40	34.915003100273466	38.0	34.0	40.0	23.0	41.0
41	34.75127160618869	38.0	34.0	40.0	23.0	41.0
42	34.73989240857075	38.0	34.0	40.0	23.0	41.0
43	34.7029402779459	38.0	34.0	40.0	23.0	41.0
44	34.513153311610836	38.0	34.0	40.0	22.0	41.0
45	34.425396221536566	38.0	34.0	40.0	22.0	41.0
46	34.321532531461806	38.0	33.0	40.0	22.0	41.0
47	34.2838253010312	38.0	33.0	40.0	22.0	41.0
48	34.195964456785156	37.0	33.0	40.0	21.0	41.0
49	34.08126586677141	37.0	33.0	40.0	20.0	41.0
50	33.930861019640005	37.0	33.0	40.0	20.0	41.0
51	32.46008799341902	35.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	48.0
9	92.0
10	114.0
11	87.0
12	56.0
13	69.0
14	78.0
15	111.0
16	215.0
17	400.0
18	822.0
19	1395.0
20	2227.0
21	3735.0
22	5501.0
23	8147.0
24	12241.0
25	18024.0
26	23387.0
27	25052.0
28	24740.0
29	25037.0
30	27617.0
31	32908.0
32	42264.0
33	57070.0
34	83772.0
35	98793.0
36	118755.0
37	176968.0
38	235860.0
39	188805.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.04968672004242	19.823716721466987	21.427377432150124	18.699219126340466
2	38.99666422103262	21.560034551786096	23.412458241004472	16.030842986176815
3	31.452006696261307	21.549494445446303	31.675983955981877	15.322514902310507
4	27.048053827005564	23.951979934272554	31.061116971300446	17.938849267421435
5	23.819240470055806	28.494518732980406	30.093403457978322	17.592837338985465
6	24.522051467009877	32.35820880774105	29.71420666348817	13.405533061760904
7	65.68075500099225	17.213722889076084	13.744381011669052	3.3611410982626113
8	76.8988042743425	5.077449195452274	14.470989592468436	3.5527569377367922
9	70.97534685596038	7.248546412287787	16.322342802136678	5.453763929615146
10	36.480460866149706	26.365911319827173	23.130263362672707	14.023364451350409
11	27.126939935392443	25.530772581560246	30.01583486288392	17.326452620163387
12	24.31355498847588	23.326737532412885	33.43412310020759	18.925584378903647
13	22.645006748138393	23.262755793147118	34.901256495958116	19.190980962756367
14	19.459918363582617	25.590472402625476	34.8439446677355	20.10566456605641
15	19.37600923576818	25.606035528392823	36.33915756553188	18.67879767030712
16	22.440380464901033	25.307454078485915	34.496038813941595	17.756126642671454
17	22.39500860089146	25.093769891362793	33.22035656850369	19.29086493924205
18	23.34353582689193	24.81421003961598	32.920375260723524	18.921878872768566
19	22.810437010924655	26.601581510018452	31.462958525504998	19.12502295355189
20	24.653061695030257	25.49281172982084	31.925817414067254	17.928309161081646
21	23.881822351448317	26.051519710410574	32.27784049690014	17.788817441240965
22	23.596169000723812	23.256909327911764	32.6259110398556	20.521010631508823
23	22.278655708249858	25.727658474204322	32.92185746317756	19.071828354368257
24	21.519438673461742	24.20576593223535	33.98369083233079	20.29110456197212
25	22.134882070208636	26.29542435867982	31.653009817944366	19.916683753167177
26	21.46080933194665	26.293448088741105	31.969048318976554	20.276694260335688
27	21.424660060984397	25.87884312451571	32.243749840457376	20.45274697404252
28	19.855831107971035	26.63707202433447	32.54743665437262	20.95966021332187
29	21.172603299217975	26.372334197127984	31.99861002347644	20.4564524801776
30	21.466408763439667	24.6706834353171	32.497618183000945	21.36528961824229
31	21.656130677555915	25.334627790143188	31.161824393593925	21.847417138706977
32	22.11478999249841	25.881066428196757	30.879464826100595	21.124678753204236
33	21.90275269699088	25.71382458463335	30.1080607933571	22.27536192501867
34	20.968882806369187	26.751201613295027	31.65515077704464	20.62476480329115
35	20.677877057894005	27.189439472204175	30.8968395326451	21.235843937256725
36	21.211222907603617	29.754226129747064	28.497977205373147	20.536573757276173
37	21.221927703104967	28.381130245246865	29.184401630752077	21.21254042089609
38	21.16074567958571	28.700544874091015	29.056849875124442	21.08185957119883
39	21.57625643419968	26.746837350513708	29.759331493755397	21.917574721531214
40	23.003617397433647	26.34334890469356	30.705553071494034	19.94748062637876
41	20.355909747045683	27.677578147065773	30.685790372106926	21.28072173378162
42	21.6149583871661	27.818387380198928	29.461655834237067	21.104998398397903
43	21.49358247509694	27.18202845993401	29.187119001917804	22.137270063051247
44	21.910410743003386	26.560079841305523	29.457126882294187	22.072382533396905
45	21.827901473062205	25.69603815518495	29.413237220738647	23.062823151014197
46	21.44326993624059	26.778622358694644	28.975658118475735	22.80244958658903
47	20.701839330900874	27.31081538427334	30.854514418124374	21.132830866701415
48	21.14732351291863	26.74840189754852	30.052148823007734	22.052125766525116
49	21.40366219288559	26.589970924128526	29.911257245293797	22.09510963769208
50	19.649063865633405	26.77829298037152	31.232558388483618	22.340084765511453
51	20.255367013913762	25.38222295783381	30.61958532916011	23.742824699092317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	377.0
1	892.5
2	1408.0
3	17915.5
4	34423.0
5	23192.0
6	11961.0
7	11051.5
8	10142.0
9	9958.0
10	9774.0
11	9525.0
12	9276.0
13	8788.5
14	8301.0
15	7889.5
16	7478.0
17	7154.0
18	6830.0
19	6770.5
20	6711.0
21	6800.5
22	6890.0
23	7362.0
24	7834.0
25	8948.0
26	11577.0
27	13092.0
28	14871.5
29	16651.0
30	19861.0
31	23071.0
32	25938.0
33	28805.0
34	32693.5
35	36582.0
36	38467.5
37	40353.0
38	45360.0
39	50367.0
40	52949.0
41	55531.0
42	59394.0
43	63257.0
44	67696.0
45	72135.0
46	81666.5
47	91198.0
48	91753.5
49	92309.0
50	90247.0
51	88185.0
52	82431.0
53	76677.0
54	70656.5
55	64636.0
56	60781.5
57	56927.0
58	54394.5
59	51862.0
60	49032.5
61	46203.0
62	42567.5
63	38932.0
64	33107.5
65	27283.0
66	22464.0
67	17645.0
68	14938.5
69	12232.0
70	10085.5
71	7939.0
72	6323.0
73	4707.0
74	3936.5
75	2395.5
76	1625.0
77	1197.0
78	769.0
79	566.5
80	364.0
81	267.0
82	170.0
83	145.0
84	120.0
85	95.5
86	71.0
87	57.5
88	44.0
89	29.0
90	14.0
91	12.0
92	10.0
93	8.5
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1214409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.36810694851103
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28650002028009	24.843501636305305
2	9.012321128851324	6.194728328213758
3	4.166438169197203	4.295777777799838
4	2.6367398052390456	3.6247902248740664
5	1.7880509921972778	3.072596386461365
6	1.2119765820724075	2.4992004475053213
7	0.9370061139488767	2.2542188434922594
8	0.7386690128660806	2.030932450698602
9	0.6016127029120064	1.8608660743736363
>10	6.529535337446088	43.46966124145303
>50	0.0590260109913529	1.3125021126545438
>100	0.028554777095726062	1.6947504402088964
>500	0.0016656952212165746	0.4151210195520869
>1k	0.0016656952212165746	0.804480982926296
>5k	0.0	0.0
>10k+	2.3795646017379636E-4	1.6268720334810214
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19893	1.638080745449021	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC	1867	0.1537373323155543	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT	1666	0.1371860715788503	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1530	0.1259872085928217	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCG	1275	0.10498934049401809	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1263	0.10400120552466262	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGC	1222	0.1006250777126981	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05105364008336565	0.0
2	0.0	0.0	0.0	0.22982372495592507	0.0
3	0.0	0.0	0.0	0.3894898670876122	0.0
4	0.0	0.0	0.0	0.6478871615740661	0.0
5	0.0	0.0	0.0	1.2817757444155964	0.0
6	0.0	0.0	0.0	1.9841750184657723	0.0
7	0.0	0.0	0.0	2.4658084714457815	0.0
8	0.0	0.0	0.0	3.1718309070502606	0.0
9	0.0	0.0	0.0	3.5882474520528094	0.0
10	0.0	0.0	0.0	4.229958770068404	0.0
11	0.0	0.0	0.0	4.736707320186198	0.0
12	0.0	0.0	0.0	5.24823185598921	0.0
13	0.0	0.0	0.0	5.476655723071881	0.0
14	0.0	0.0	0.0	5.614994618781646	0.0
15	0.0	0.0	0.0	5.776554686271265	0.0
16	8.234458077962202E-5	0.0	0.0	6.004484485869258	0.0
17	8.234458077962202E-5	0.0	0.0	6.273339542114724	0.0
18	8.234458077962202E-5	0.0	0.0	6.567145006336416	0.0
19	8.234458077962202E-5	0.0	0.0	6.7730064582854705	0.0
20	8.234458077962202E-5	0.0	0.0	6.9803501126885585	0.0
21	8.234458077962202E-5	0.0	0.0	7.231748117808745	0.0
22	8.234458077962202E-5	0.0	0.0	7.508672942970613	0.0
23	8.234458077962202E-5	0.0	0.0	7.781398194512722	0.0
24	8.234458077962202E-5	0.0	0.0	8.009492683272274	0.0
25	1.6468916155924405E-4	0.0	0.0	8.23470511170454	0.0
26	1.6468916155924405E-4	0.0	0.0	8.449048055473897	0.0
27	2.4703374233886607E-4	0.0	0.0	8.663308654662474	0.0
28	2.4703374233886607E-4	0.0	0.0	8.882839307020946	0.0
29	2.4703374233886607E-4	0.0	0.0	9.121803280443409	0.0
30	2.4703374233886607E-4	0.0	0.0	9.427136985974247	0.0
31	2.4703374233886607E-4	0.0	0.0	9.680922983937043	0.0
32	3.293783231184881E-4	0.0	0.0	9.97332859028548	0.0
33	3.293783231184881E-4	0.0	0.0	10.243418815242642	0.0
34	4.117229038981101E-4	0.0	0.0	10.495146198685946	0.0
35	4.117229038981101E-4	0.0	0.0	10.762024984992701	0.0
36	4.940674846777321E-4	0.0	0.0	11.027833291749321	0.0
37	4.940674846777321E-4	0.0	0.0	11.299488063741293	0.0
38	4.940674846777321E-4	0.0	0.0	11.59197601467051	0.0
39	4.940674846777321E-4	0.0	0.0	11.93189444412879	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	35	1.2120108E-7	45.000004	1
CGTATAG	25	3.890937E-5	45.0	2
CGTTTTT	10080	0.0	43.794643	1
CGTTGAT	85	0.0	42.352943	25
CGTGCGG	155	0.0	40.64516	2
TCGTTGA	90	0.0	39.999996	24
CAACGTT	45	1.9284926E-8	39.999996	8
TCGTAAG	45	1.9284926E-8	39.999996	1
CGTTTTA	665	0.0	39.92481	1
GCTACGA	195	0.0	39.23077	10
CGAATAT	190	0.0	39.078945	14
TCGACGT	35	6.2485797E-6	38.57143	26
ACACGAC	245	0.0	37.65306	26
TACCGCG	30	1.13996015E-4	37.499996	25
TACGGGA	295	0.0	37.372883	4
TACGAAT	205	0.0	37.317074	12
AAACACG	250	0.0	36.899998	40
ACGACCA	250	0.0	36.899998	28
ACAACGA	435	0.0	36.72414	13
GTTTTTT	12320	0.0	36.672077	2
>>END_MODULE