FastQCFastQC Report
Sat 14 Jan 2017
SRR2936635.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936635.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857865
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC203012.3664562606004442TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178162.07678364311401No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG101701.185501215226172No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC87021.0143787192623548No Hit
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT66240.7721494640765155TruSeq Adapter, Index 27 (100% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC34750.40507539065004394TruSeq Adapter, Index 27 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT28710.33466804217446805No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT21200.2471251304109621No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC19060.22217948045438388No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT17650.20574332791290004TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.16459466233032005No Hit
GAATCTATCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC13350.15561889108426152TruSeq Adapter, Index 27 (100% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC11040.1286915773460859TruSeq Adapter, Index 27 (100% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT10280.1198323745577684No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAT207.0322864E-445.040
CGTTTTT95400.044.1037751
CGTTTTA6100.042.7868841
ACGGGTC900.042.55
TACGGCT10750.041.2325557
TGATACC13450.040.985134
GCGATAT556.002665E-1140.9090929
GAATCTG27150.040.3591161
ACGGCTG11750.039.829798
CGCACGG403.456862E-739.3752
ATACGGT403.456862E-739.37528
GATACCT14200.038.8204235
AACCCGC700.038.57142621
ATACGGC21700.038.3640566
AATCTGT28550.038.3012282
GTTTTTT111500.038.1390152
GAATGAT39550.037.718081
CGGCTGT12250.037.6530659
GTCGACG301.13968075E-437.4999961
ATGATAC37350.037.469883
Produced by FastQC (version 0.11.5)