##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936635.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	857865
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.979324252650475	31.0	30.0	33.0	28.0	34.0
2	31.05671754879847	31.0	31.0	34.0	28.0	34.0
3	30.63947007979111	31.0	30.0	34.0	27.0	34.0
4	34.595744085607876	35.0	35.0	37.0	32.0	37.0
5	34.93064060195952	35.0	35.0	37.0	32.0	37.0
6	34.88938236202666	35.0	35.0	37.0	32.0	37.0
7	31.08649262995926	35.0	35.0	37.0	0.0	37.0
8	32.86111334533989	35.0	33.0	37.0	17.0	37.0
9	36.05308061291695	38.0	35.0	39.0	32.0	39.0
10	35.96531272403001	37.0	35.0	39.0	32.0	39.0
11	36.2500335134316	37.0	35.0	39.0	32.0	39.0
12	36.42443274874252	38.0	35.0	39.0	32.0	39.0
13	36.377705116772454	38.0	35.0	39.0	32.0	39.0
14	37.5828166436444	39.0	36.0	41.0	32.0	41.0
15	37.58913698542312	39.0	36.0	41.0	32.0	41.0
16	37.60237216811503	39.0	36.0	41.0	32.0	41.0
17	37.35847948103723	39.0	36.0	41.0	32.0	41.0
18	37.10597471630152	39.0	36.0	40.0	32.0	41.0
19	36.828922965734705	38.0	36.0	40.0	31.0	41.0
20	36.57504852162054	38.0	35.0	40.0	31.0	41.0
21	36.70840516864541	38.0	35.0	40.0	31.0	41.0
22	36.78797946063774	38.0	35.0	40.0	31.0	41.0
23	36.88583052111929	38.0	35.0	40.0	32.0	41.0
24	36.70512726361374	38.0	35.0	40.0	31.0	41.0
25	36.46988861883863	38.0	35.0	40.0	31.0	41.0
26	36.35387735832561	38.0	35.0	40.0	31.0	41.0
27	36.44924784202642	38.0	35.0	40.0	31.0	41.0
28	36.39461104019863	38.0	35.0	40.0	31.0	41.0
29	36.28780169373969	38.0	35.0	40.0	30.0	41.0
30	36.01608644716826	38.0	35.0	40.0	30.0	41.0
31	35.9664690831308	38.0	35.0	40.0	30.0	41.0
32	35.552125334405765	38.0	35.0	40.0	27.0	41.0
33	35.12814020854097	38.0	34.0	40.0	25.0	41.0
34	34.87041317689846	38.0	34.0	40.0	23.0	41.0
35	34.64180611168424	38.0	34.0	40.0	21.0	41.0
36	34.49902373916642	38.0	34.0	40.0	20.0	41.0
37	34.373379261305686	38.0	34.0	40.0	18.0	41.0
38	34.150311529203314	38.0	33.0	40.0	18.0	41.0
39	33.839813956741445	38.0	33.0	40.0	16.0	41.0
40	33.564298578447655	38.0	33.0	40.0	15.0	41.0
41	33.367064747949854	38.0	33.0	40.0	15.0	41.0
42	33.44846566767498	38.0	33.0	40.0	15.0	41.0
43	33.36582096250576	38.0	33.0	40.0	15.0	41.0
44	33.20649053172702	37.0	32.0	40.0	14.0	41.0
45	33.181985510540706	37.0	32.0	40.0	15.0	41.0
46	32.948424285872484	37.0	31.0	40.0	13.0	41.0
47	32.80632617020161	37.0	31.0	40.0	13.0	41.0
48	32.76563212160421	37.0	31.0	40.0	12.0	41.0
49	32.669486457659424	37.0	31.0	40.0	12.0	41.0
50	32.559522768734006	36.0	31.0	40.0	12.0	41.0
51	30.92308580021332	35.0	27.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	27.0
9	55.0
10	47.0
11	43.0
12	35.0
13	33.0
14	39.0
15	64.0
16	144.0
17	365.0
18	706.0
19	1372.0
20	2433.0
21	3641.0
22	5248.0
23	7782.0
24	11827.0
25	16986.0
26	21832.0
27	23426.0
28	22981.0
29	22904.0
30	24360.0
31	28392.0
32	35012.0
33	44559.0
34	60783.0
35	70742.0
36	79698.0
37	112622.0
38	146485.0
39	113217.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97373712647095	17.2065534786942	19.06512096891702	21.75458842591783
2	37.97019344535562	27.30709377349583	20.596830503634024	14.125882277514526
3	26.291432801198322	27.456767673235298	33.13446754442715	13.117331981139223
4	23.355539624532998	20.845471023995618	39.72874519883665	16.07024415263474
5	25.464029888152567	23.877766315212767	32.718900992580416	17.93930280405425
6	21.676370990773606	32.172428062690514	33.56588740652667	12.585313540009208
7	58.432737085671995	16.054274273924218	21.8997161558054	3.613272484598393
8	66.9628671177866	7.740145594003718	20.549969983622134	4.747017304587551
9	62.055801320720626	5.4596002867584055	20.97964131885553	11.504957073665437
10	33.88761634989188	20.68227518315819	29.75281658536017	15.677291881589762
11	25.9159657988145	23.554405413439177	32.65338951932997	17.87623926841636
12	22.134718166611297	20.645789255885248	37.98791185093226	19.231580726571195
13	20.488305269477134	21.092246449033354	41.32223601615639	17.097212265333123
14	18.424810430545598	25.688773874677253	36.83458353004261	19.051832164734545
15	17.731344675444273	23.86331182645288	41.475057264254865	16.930286233847983
16	20.34970537322306	25.31960156901144	34.51708602169339	19.81360703607211
17	20.274751854895584	24.994142434998516	35.78092124052153	18.950184469584375
18	20.139532443916	25.365412972903663	35.227570771624904	19.26748381155543
19	19.34966457426285	26.42944985516369	33.32867059502369	20.892214975549766
20	21.01496156155106	26.28886829512802	36.282748451096616	16.4134216922243
21	20.93930863247714	26.64533463890006	35.820904221526696	16.594452507096104
22	20.132888041824764	21.519819552027418	36.796815349734516	21.550477056413307
23	20.336999411329288	24.841204618442294	37.00850366899221	17.81329230123621
24	22.204775809713652	23.312875568999782	34.772487512603966	19.709861108682603
25	19.688295944000515	28.450746912392976	32.80003263916817	19.060924504438344
26	19.484883985242433	23.37757106304605	34.641697703018544	22.495847248692975
27	21.844462706836158	24.391133803104218	32.88745898247393	20.876944507585694
28	18.08326484936441	23.94560915761804	35.33994276488725	22.6311832281303
29	20.002098232239337	23.732288879951973	32.730907543727746	23.534705344080944
30	18.983289911582823	26.575976406544154	34.53748550179807	19.903248180074954
31	19.88692859599121	22.856743193859174	32.393674995482975	24.862653214666643
32	20.57713043427579	23.79348732026601	33.24066140942922	22.38872083602898
33	18.95682887167561	22.613814527926888	35.40627021734189	23.023086383055606
34	19.145436636300584	23.023552656886572	37.42710100074021	20.403909706072636
35	18.007262214917265	25.35725318086179	35.217779021174664	21.417705583046285
36	18.83641365482914	25.267029194570238	31.483858182814313	24.412698967786305
37	18.59558322113619	25.791470685947086	33.74878331672233	21.86416277619439
38	19.730610294160503	25.259452244817076	31.24699107668456	23.762946384337862
39	21.884795393214553	23.848857337692994	32.186299709161695	22.080047559930758
40	20.51325091943371	24.353948465084834	35.62308754874019	19.50971306674127
41	18.67892966842102	27.184347187494534	31.953978772883847	22.182744371200595
42	20.080898509672267	25.25374039038776	34.73168855239461	19.93367254754536
43	22.48407383446113	24.417594843011432	29.676114540166576	23.42221678236086
44	21.151230088650312	23.85981477272065	30.942397696607276	24.04655744202176
45	20.484458510371677	23.633905101618552	30.253011837526884	25.628624550482886
46	23.567461080706174	24.94809789419081	30.095644419576505	21.38879660552651
47	19.26643469543576	23.92147948686565	35.74140453334732	21.070681284351267
48	20.20212970572293	24.659008118993082	31.154319152780445	23.98454302250354
49	19.203954002086576	23.160986868563235	35.59709278266394	22.03796634668625
50	19.32332010281338	23.123218688255147	34.83671673281927	22.716744476112208
51	19.406083707809504	22.807201599319242	32.157390731641925	25.629323961229332
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	169.0
1	482.5
2	796.0
3	16243.0
4	31690.0
5	21477.0
6	11264.0
7	10484.0
8	9704.0
9	9606.0
10	9508.0
11	9168.5
12	8829.0
13	8487.0
14	8145.0
15	7640.0
16	7135.0
17	6714.0
18	6293.0
19	5909.5
20	5526.0
21	5499.0
22	5472.0
23	5540.0
24	5608.0
25	5927.0
26	7066.5
27	7887.0
28	9400.5
29	10914.0
30	12768.5
31	14623.0
32	16482.5
33	18342.0
34	20057.5
35	21773.0
36	23777.0
37	25781.0
38	28812.5
39	31844.0
40	33251.5
41	34659.0
42	37710.0
43	40761.0
44	45217.5
45	49674.0
46	82128.0
47	114582.0
48	89663.5
49	64745.0
50	63339.5
51	61934.0
52	55080.0
53	48226.0
54	44970.5
55	41715.0
56	38946.0
57	36177.0
58	33147.5
59	30118.0
60	28092.0
61	26066.0
62	23563.0
63	21060.0
64	18124.0
65	15188.0
66	12530.0
67	9872.0
68	8094.0
69	6316.0
70	5010.0
71	3704.0
72	3020.0
73	2336.0
74	1964.0
75	1192.5
76	793.0
77	578.5
78	364.0
79	281.5
80	199.0
81	145.5
82	92.0
83	71.5
84	51.0
85	51.0
86	51.0
87	34.0
88	17.0
89	13.0
90	9.0
91	8.5
92	8.0
93	7.0
94	6.0
95	3.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	857865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.66486052565919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.1436058360406	30.475181355024233
2	9.892248746628903	8.243183286268405
3	4.4870159096803715	5.608526761597394
4	2.608609970783467	4.347494823941482
5	1.7639261219330782	3.6746867923954305
6	1.373496508647957	3.433592427917819
7	1.1226813132814353	3.2743452232844423
8	0.9399449092083358	3.1330138835174965
9	0.8073181727007143	3.027311915886477
>10	3.8034954839911594	22.603209970581638
>50	0.036415092373408835	1.0451623204877318
>100	0.015448679804597482	1.3565283907442802
>500	0.0019310849753190224	0.5102211088051889
>1k	0.002482823539695886	1.9558284269946147
>5k	5.517385643768636E-4	1.7615788946943738
>10k+	8.276078465652952E-4	5.550134417859013
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	20301	2.3664562606004442	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17816	2.07678364311401	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	10170	1.185501215226172	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC	8702	1.0143787192623548	No Hit
GAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	6624	0.7721494640765155	TruSeq Adapter, Index 27 (100% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	3475	0.40507539065004394	TruSeq Adapter, Index 27 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	2871	0.33466804217446805	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT	2120	0.2471251304109621	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC	1906	0.22217948045438388	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	1765	0.20574332791290004	TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1412	0.16459466233032005	No Hit
GAATCTATCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	1335	0.15561889108426152	TruSeq Adapter, Index 27 (100% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	1104	0.1286915773460859	TruSeq Adapter, Index 27 (100% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT	1028	0.1198323745577684	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1656845774101985E-4	0.0	0.0	0.058983639616956046	0.0
2	1.1656845774101985E-4	0.0	0.0	0.6889195852494273	0.0
3	1.1656845774101985E-4	0.0	0.0	0.9303328612310795	0.0
4	1.1656845774101985E-4	0.0	0.0	2.049506624002611	0.0
5	1.1656845774101985E-4	0.0	0.0	5.331724688616507	0.0
6	1.1656845774101985E-4	0.0	0.0	6.513728850110448	0.0
7	1.1656845774101985E-4	0.0	0.0	7.319566598474119	0.0
8	1.1656845774101985E-4	0.0	0.0	8.182406322673147	0.0
9	1.1656845774101985E-4	0.0	0.0	8.582236132724846	0.0
10	1.1656845774101985E-4	0.0	0.0	10.47659013947416	0.0
11	1.1656845774101985E-4	0.0	0.0	10.927710070931907	0.0
12	1.1656845774101985E-4	0.0	0.0	12.458720194902462	0.0
13	1.1656845774101985E-4	0.0	0.0	12.703980229989567	0.0
14	1.1656845774101985E-4	0.0	0.0	12.869390871524073	0.0
15	1.1656845774101985E-4	0.0	0.0	13.110104736759281	0.0
16	1.1656845774101985E-4	0.0	0.0	13.344057631445507	0.0
17	1.1656845774101985E-4	0.0	0.0	13.579409347624626	0.0
18	1.1656845774101985E-4	0.0	0.0	13.805668724099945	0.0
19	1.1656845774101985E-4	0.0	0.0	14.01537537957604	0.0
20	1.1656845774101985E-4	0.0	0.0	14.193258846088836	0.0
21	1.1656845774101985E-4	0.0	0.0	14.367878395784885	0.0
22	1.1656845774101985E-4	0.0	0.0	14.574670839817454	0.0
23	1.1656845774101985E-4	0.0	0.0	14.770622417280109	0.0
24	1.1656845774101985E-4	0.0	0.0	14.94361000856778	0.0
25	1.1656845774101985E-4	0.0	0.0	15.107738397067138	0.0
26	1.1656845774101985E-4	0.0	0.0	15.270701100989083	0.0
27	1.1656845774101985E-4	0.0	0.0	15.454063285015708	0.0
28	1.1656845774101985E-4	0.0	0.0	15.62576862326823	0.0
29	1.1656845774101985E-4	0.0	0.0	15.809363944210336	0.0
30	1.1656845774101985E-4	0.0	0.0	16.02000314734836	0.0
31	1.1656845774101985E-4	0.0	0.0	16.204880721325615	0.0
32	2.331369154820397E-4	0.0	0.0	16.415170219090417	0.0
33	2.331369154820397E-4	0.0	0.0	16.624993443024252	0.0
34	2.331369154820397E-4	0.0	0.0	16.818380514416603	0.0
35	2.331369154820397E-4	0.0	0.0	17.018411987900194	0.0
36	2.331369154820397E-4	0.0	0.0	17.222873062777943	0.0
37	2.331369154820397E-4	0.0	0.0	17.4304814860147	0.0
38	2.331369154820397E-4	0.0	0.0	17.678888869460813	0.0
39	2.331369154820397E-4	0.0	0.0	17.95737091500411	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCGAT	20	7.0322864E-4	45.0	40
CGTTTTT	9540	0.0	44.103775	1
CGTTTTA	610	0.0	42.786884	1
ACGGGTC	90	0.0	42.5	5
TACGGCT	1075	0.0	41.232555	7
TGATACC	1345	0.0	40.98513	4
GCGATAT	55	6.002665E-11	40.909092	9
GAATCTG	2715	0.0	40.359116	1
ACGGCTG	1175	0.0	39.82979	8
CGCACGG	40	3.456862E-7	39.375	2
ATACGGT	40	3.456862E-7	39.375	28
GATACCT	1420	0.0	38.820423	5
AACCCGC	70	0.0	38.571426	21
ATACGGC	2170	0.0	38.364056	6
AATCTGT	2855	0.0	38.301228	2
GTTTTTT	11150	0.0	38.139015	2
GAATGAT	3955	0.0	37.71808	1
CGGCTGT	1225	0.0	37.653065	9
GTCGACG	30	1.13968075E-4	37.499996	1
ATGATAC	3735	0.0	37.46988	3
>>END_MODULE