##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936634.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	919039
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.122395241115992	31.0	31.0	33.0	28.0	34.0
2	31.18593008566557	31.0	31.0	34.0	28.0	34.0
3	30.875146756557665	31.0	31.0	34.0	27.0	34.0
4	34.84247458486528	35.0	35.0	37.0	32.0	37.0
5	35.117408510411416	35.0	35.0	37.0	33.0	37.0
6	35.097721641845446	36.0	35.0	37.0	32.0	37.0
7	31.029800693985784	35.0	35.0	37.0	0.0	37.0
8	33.03838683668484	35.0	35.0	37.0	17.0	37.0
9	36.36368206354681	39.0	35.0	39.0	32.0	39.0
10	36.113763398506485	37.0	35.0	39.0	32.0	39.0
11	36.39477976451489	38.0	35.0	39.0	32.0	39.0
12	36.5085660129766	38.0	35.0	39.0	32.0	39.0
13	36.48835141925424	39.0	35.0	39.0	32.0	39.0
14	37.551595742944535	39.0	36.0	41.0	32.0	41.0
15	37.69918033946329	40.0	37.0	41.0	33.0	41.0
16	37.658913277891365	39.0	36.0	41.0	33.0	41.0
17	37.5164111642705	39.0	36.0	41.0	32.0	41.0
18	37.40151397274762	39.0	36.0	40.0	32.0	41.0
19	37.15824464467776	39.0	36.0	40.0	32.0	41.0
20	37.06014652261765	39.0	35.0	40.0	32.0	41.0
21	37.08229248160307	39.0	35.0	40.0	32.0	41.0
22	37.184713597573115	39.0	35.0	40.0	32.0	41.0
23	37.235247905692795	39.0	35.0	40.0	32.0	41.0
24	37.140384684436675	39.0	35.0	40.0	32.0	41.0
25	36.8289659089549	39.0	35.0	40.0	31.0	41.0
26	36.81737010072478	39.0	35.0	40.0	31.0	41.0
27	36.86657584716209	39.0	35.0	40.0	31.0	41.0
28	36.842812981821226	39.0	35.0	40.0	31.0	41.0
29	36.80473298739226	39.0	35.0	40.0	31.0	41.0
30	36.54655025521224	39.0	35.0	40.0	31.0	41.0
31	36.48247789266832	39.0	35.0	40.0	30.0	41.0
32	36.257500497802596	39.0	35.0	40.0	30.0	41.0
33	36.018586806435856	39.0	35.0	40.0	29.0	41.0
34	35.85899836677225	39.0	35.0	40.0	28.0	41.0
35	35.708967736951315	39.0	35.0	40.0	27.0	41.0
36	35.62941289760282	39.0	35.0	40.0	27.0	41.0
37	35.5306717125171	38.0	35.0	40.0	26.0	41.0
38	35.39186693927026	38.0	35.0	40.0	25.0	41.0
39	35.33353753213955	38.0	35.0	40.0	25.0	41.0
40	35.23597366379446	38.0	35.0	40.0	24.0	41.0
41	35.07934483737905	38.0	34.0	40.0	24.0	41.0
42	35.093928549278104	38.0	34.0	40.0	24.0	41.0
43	35.062482658516124	38.0	34.0	40.0	24.0	41.0
44	34.88501249674932	38.0	34.0	40.0	23.0	41.0
45	34.781891736912144	38.0	34.0	40.0	23.0	41.0
46	34.723951867113364	38.0	34.0	40.0	23.0	41.0
47	34.65837031943149	38.0	34.0	40.0	23.0	41.0
48	34.596974665928215	38.0	34.0	40.0	23.0	41.0
49	34.5081830042033	38.0	34.0	40.0	23.0	41.0
50	34.372367222718516	37.0	34.0	40.0	23.0	41.0
51	32.88480575905919	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	30.0
9	77.0
10	86.0
11	64.0
12	43.0
13	29.0
14	48.0
15	84.0
16	166.0
17	330.0
18	599.0
19	1059.0
20	1807.0
21	2655.0
22	4036.0
23	5703.0
24	8533.0
25	12135.0
26	15150.0
27	16780.0
28	17225.0
29	18427.0
30	20790.0
31	25834.0
32	33202.0
33	44885.0
34	65764.0
35	74452.0
36	89068.0
37	133815.0
38	179697.0
39	146460.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61749392572023	19.94855495795064	22.57455885985252	15.859392256476601
2	36.161577473861286	24.005945340730918	23.785280058844073	16.047197126563724
3	31.32979122757576	23.74687037220401	29.845305803126966	15.07803259709327
4	27.165114864548727	24.517784337770216	30.71033982235792	17.606760975323134
5	25.69401298530313	28.022967469280413	28.63447579482481	17.64854375059165
6	25.023421204105595	33.74144078760531	28.213710190753606	13.021427817535491
7	66.05671794124079	17.479018844684504	13.080076035946245	3.384187178128458
8	77.40454975251322	6.409521249914313	12.628190969044839	3.557738028527625
9	72.05842189504472	6.6866585640000045	14.438560278725928	6.8163592622293505
10	37.60417131373097	24.604505358314498	22.72917689020814	15.062146437746385
11	29.129340539411274	26.471999556058012	27.598502348648967	16.800157555881743
12	25.483793397233413	23.846213272777327	31.757738246146243	18.912255083843014
13	23.381162279293914	24.063179038103932	33.64938811084187	18.906270571760285
14	20.53394904895222	26.249919753133437	33.26964361686501	19.946487581049336
15	20.51360170787094	25.765500702364104	35.53320370517464	18.187693884590317
16	24.260776746144614	25.481073164468537	31.24383187220564	19.01431821718121
17	23.870586558350627	25.698800594969313	31.224028577677338	19.20658426900273
18	24.337922547356534	24.873808402037344	32.10984517523195	18.67842387537417
19	23.380727042051536	26.657410621312046	30.46388673386004	19.49797560277638
20	24.27154886789353	27.072191713300526	30.92491178285143	17.731347635954513
21	24.107899664758513	27.349764264628597	31.615850905130248	16.926485165482642
22	23.266368456616096	23.572993093873055	32.47631493331621	20.684323516194635
23	22.04367823345908	26.007057371885196	33.066496633983974	18.882767760671744
24	21.810935118096186	25.62317812410572	32.38948510346133	20.176401654336757
25	21.800924661521435	27.663352697763642	30.796952033591612	19.73877060712331
26	21.164390194540168	26.15231780152964	30.99683473715479	21.686457266775403
27	21.787867544250027	26.48930023644263	31.07136911491242	20.651463104394917
28	19.871517966049318	26.816272214780874	32.23791373380237	21.074296085367433
29	21.27309069582466	26.03436850884456	31.498663277619336	21.193877517711435
30	21.987967866434396	26.810940558561715	31.464605963403073	19.736485611600813
31	23.005987776362048	26.060047506145008	30.269770923758404	20.66419379373454
32	23.186937659881682	26.846303584505122	29.63780644782213	20.328952307791074
33	21.978175028480837	27.627010388024882	29.097241792785727	21.297572790708553
34	21.826930086753663	27.423101739969685	30.334403654251886	20.415564519024766
35	21.64641544047641	28.734797979193484	28.723264192270403	20.8955223880597
36	22.67760127698607	29.58623083460005	27.788918642190374	19.9472492462235
37	21.481895762856638	29.24533126450564	28.526536958714484	20.746236013923237
38	22.527335619054252	29.234667952067323	27.835053789882693	20.402942638995732
39	22.39067112494682	27.55704600131224	28.20957543695099	21.84270743678995
40	23.719559235244642	27.132145643438417	29.21595275064497	19.932342370671975
41	20.983440311020534	28.681590226312487	28.54362002047791	21.79134944218907
42	22.290240131267552	26.910501077756223	30.035069240804795	20.76418955017143
43	22.081435064235578	27.14313538380852	28.80563284039089	21.969796711565014
44	22.442790784721865	26.444906037719836	29.184942097125365	21.92736108043293
45	21.668068493284835	26.314443674316323	28.80291260762601	23.214575224772833
46	22.582284320904773	26.930630800216314	28.648294577270388	21.83879030160853
47	20.77311191364023	27.07654408572433	31.39518562324341	20.755158377392036
48	21.552404196122254	27.736472554483544	29.090277996907638	21.620845252486564
49	21.46960031075939	26.494958320593575	30.355295041886144	21.680146326760887
50	20.94231039161559	26.76175875017274	30.21873935708931	22.077191501122368
51	20.764515978103216	26.304868454983954	29.30680852499187	23.623807041920962
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	717.5
2	1179.0
3	10330.0
4	19481.0
5	13164.5
6	6848.0
7	6295.5
8	5743.0
9	5615.5
10	5488.0
11	5375.0
12	5262.0
13	5172.0
14	5082.0
15	4910.5
16	4739.0
17	4623.0
18	4507.0
19	4480.0
20	4453.0
21	4572.5
22	4692.0
23	5008.0
24	5324.0
25	6074.5
26	8361.0
27	9897.0
28	11390.5
29	12884.0
30	15017.0
31	17150.0
32	19579.5
33	22009.0
34	25458.5
35	28908.0
36	31344.5
37	33781.0
38	36241.0
39	38701.0
40	41808.5
41	44916.0
42	47635.0
43	50354.0
44	54593.0
45	58832.0
46	71638.0
47	84444.0
48	78284.5
49	72125.0
50	71205.5
51	70286.0
52	63844.0
53	57402.0
54	53801.5
55	50201.0
56	47134.5
57	44068.0
58	41324.5
59	38581.0
60	34993.0
61	31405.0
62	27475.0
63	23545.0
64	20328.0
65	17111.0
66	14587.5
67	12064.0
68	10202.5
69	8341.0
70	6755.0
71	5169.0
72	4178.5
73	3188.0
74	2539.5
75	1429.5
76	968.0
77	756.0
78	544.0
79	359.0
80	174.0
81	134.5
82	95.0
83	74.5
84	54.0
85	42.5
86	31.0
87	22.5
88	14.0
89	14.0
90	14.0
91	9.0
92	4.0
93	3.5
94	3.0
95	3.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	919039.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.351514870166895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.17642866911574	24.793696627026694
2	9.210779099347368	6.328084303941071
3	4.221641716929433	4.35059364646855
4	2.6115633328430756	3.588446266501661
5	1.725219238478233	2.9631947162441516
6	1.1877696277235137	2.4481011617446127
7	0.8828568985402829	2.1229230314905165
8	0.693245192259195	1.9051218024450756
9	0.5748763214782144	1.7773085255189156
>10	6.643478133061017	43.6583429826099
>50	0.04613365001855482	1.0349862648575985
>100	0.02074457206875506	1.268973103698957
>500	0.0034058252649025954	0.8047146511100285
>1k	0.0012384819145100346	1.2182397057645078
>5k	3.0962047862750865E-4	0.5362637154238388
>10k+	3.0962047862750865E-4	1.2010094951539045
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11292	1.2286747352397451	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC	5042	0.5486165440204387	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	4332	0.4713619334979256	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	4311	0.4690769379754287	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1465	0.1594056400218054	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT	1346	0.1464573320609898	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTT	935	0.10173670540640821	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.26427931785267E-4	0.0	0.0	0.05745131599420699	0.0
2	3.26427931785267E-4	0.0	0.0	0.33393577421632814	0.0
3	3.26427931785267E-4	0.0	0.0	0.4993259263208634	0.0
4	3.26427931785267E-4	0.0	0.0	0.8112822197969836	0.0
5	3.26427931785267E-4	0.0	0.0	1.781099605131012	0.0
6	3.26427931785267E-4	0.0	0.0	2.3120890408350463	0.0
7	3.26427931785267E-4	0.0	0.0	2.7292639376566172	0.0
8	3.26427931785267E-4	0.0	0.0	3.3103056562343927	0.0
9	3.26427931785267E-4	0.0	0.0	3.5903808217061517	0.0
10	3.26427931785267E-4	0.0	0.0	4.550514178397217	0.0
11	3.26427931785267E-4	0.0	0.0	5.013388985668725	0.0
12	3.26427931785267E-4	0.0	0.0	5.896485350458468	0.0
13	3.26427931785267E-4	0.0	0.0	6.095062342294505	0.0
14	3.26427931785267E-4	0.0	0.0	6.217908053956361	0.0
15	3.26427931785267E-4	0.0	0.0	6.410282915088478	0.0
16	3.26427931785267E-4	0.0	0.0	6.6487929239129135	0.0
17	3.26427931785267E-4	0.0	0.0	6.914178832454335	0.0
18	3.26427931785267E-4	0.0	0.0	7.211554678310714	0.0
19	3.26427931785267E-4	0.0	0.0	7.413504758775199	0.0
20	3.26427931785267E-4	0.0	0.0	7.6158900764820645	0.0
21	3.26427931785267E-4	0.0	0.0	7.836120121126524	0.0
22	3.26427931785267E-4	0.0	0.0	8.086490344805824	0.0
23	3.26427931785267E-4	0.0	0.0	8.327285349152756	0.0
24	3.26427931785267E-4	0.0	0.0	8.537396127911872	0.0
25	3.26427931785267E-4	0.0	0.0	8.729444561112205	0.0
26	3.26427931785267E-4	0.0	0.0	8.914964435676833	0.0
27	3.26427931785267E-4	0.0	0.0	9.120722842012146	0.0
28	3.26427931785267E-4	0.0	0.0	9.305045814160225	0.0
29	3.26427931785267E-4	0.0	0.0	9.528322519501348	0.0
30	3.26427931785267E-4	0.0	0.0	9.802848410132759	0.0
31	3.26427931785267E-4	0.0	0.0	10.035047478942678	0.0
32	3.26427931785267E-4	0.0	0.0	10.271272492244616	0.0
33	3.26427931785267E-4	0.0	0.0	10.498357523456567	0.0
34	3.26427931785267E-4	0.0	0.0	10.735670629864456	0.0
35	3.26427931785267E-4	0.0	0.0	10.980709197324597	0.0
36	3.26427931785267E-4	0.0	0.0	11.212255410270947	0.0
37	3.26427931785267E-4	0.0	0.0	11.466107531889289	0.0
38	3.26427931785267E-4	0.0	0.0	11.739980566657128	0.0
39	3.26427931785267E-4	0.0	0.0	12.12919147065576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTCACG	20	7.0325466E-4	45.000004	42
TTACCCG	20	7.0325466E-4	45.000004	12
CGTTTTT	5600	0.0	43.433037	1
CGAATAT	95	0.0	42.63158	14
CGCACGG	55	6.002665E-11	40.909092	2
AATAGCG	50	1.0804797E-9	40.500004	1
GACCGAT	340	0.0	39.705883	9
CGTTTTA	370	0.0	38.918922	1
ACGGGTA	105	0.0	38.57143	5
GTATGCG	35	6.2469226E-6	38.571426	1
TATCCGG	35	6.2469226E-6	38.571426	2
TACGGCT	480	0.0	38.437496	7
CACGACC	230	0.0	38.152172	27
TACGGGT	95	0.0	37.894737	4
TCTAGCG	30	1.139744E-4	37.499996	1
AGTTGCG	30	1.139744E-4	37.499996	1
GCGTACG	30	1.139744E-4	37.499996	1
CACGTGA	230	0.0	37.173912	43
TCAAGCG	255	0.0	37.058823	17
AGCGACT	225	0.0	37.0	19
>>END_MODULE