##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936633.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	324980
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217428764847067	31.0	31.0	33.0	28.0	34.0
2	31.241753338666996	31.0	31.0	34.0	28.0	34.0
3	30.99113483906702	31.0	31.0	34.0	28.0	34.0
4	34.94631361929965	35.0	35.0	37.0	32.0	37.0
5	35.22561388393132	35.0	35.0	37.0	33.0	37.0
6	35.22196135146778	36.0	35.0	37.0	33.0	37.0
7	30.649729214105484	35.0	35.0	37.0	0.0	37.0
8	32.88816234845221	36.0	35.0	37.0	17.0	37.0
9	36.22037356144993	39.0	35.0	39.0	32.0	39.0
10	36.19211951504708	37.0	35.0	39.0	32.0	39.0
11	36.49015016308696	38.0	35.0	39.0	32.0	39.0
12	36.54403347898332	38.0	35.0	39.0	32.0	39.0
13	36.428161733029725	39.0	35.0	39.0	32.0	39.0
14	37.49523662994646	39.0	36.0	41.0	32.0	41.0
15	37.72703858698997	39.0	36.0	41.0	33.0	41.0
16	37.64129177180134	39.0	36.0	41.0	33.0	41.0
17	37.55460643731922	39.0	36.0	41.0	33.0	41.0
18	37.458511293002644	39.0	36.0	40.0	33.0	41.0
19	37.19015016308696	39.0	36.0	40.0	32.0	41.0
20	37.11361929964921	39.0	35.0	40.0	32.0	41.0
21	37.11317619545818	39.0	35.0	40.0	32.0	41.0
22	37.163803926395474	39.0	35.0	40.0	32.0	41.0
23	37.22009662133054	39.0	35.0	40.0	32.0	41.0
24	37.16867191827189	39.0	35.0	40.0	32.0	41.0
25	36.84484275955443	39.0	35.0	40.0	31.0	41.0
26	36.794750446181304	39.0	35.0	40.0	31.0	41.0
27	36.81036986891501	39.0	35.0	40.0	31.0	41.0
28	36.78008800541572	39.0	35.0	40.0	31.0	41.0
29	36.74956612714629	39.0	35.0	40.0	31.0	41.0
30	36.54173179887993	38.0	35.0	40.0	31.0	41.0
31	36.454997230598806	38.0	35.0	40.0	30.0	41.0
32	36.24380577266293	38.0	35.0	40.0	30.0	41.0
33	36.070373561449934	38.0	35.0	40.0	30.0	41.0
34	35.91110529878762	38.0	35.0	40.0	29.0	41.0
35	35.81518554987999	38.0	35.0	40.0	29.0	41.0
36	35.6855160317558	38.0	35.0	40.0	28.0	41.0
37	35.529863376207764	38.0	35.0	40.0	27.0	41.0
38	35.40708966705643	38.0	35.0	40.0	26.0	41.0
39	35.39444888916241	38.0	35.0	40.0	26.0	41.0
40	35.23832235829897	38.0	34.0	40.0	25.0	41.0
41	35.09471967505693	38.0	34.0	40.0	24.0	41.0
42	35.07413686996123	38.0	34.0	40.0	24.0	41.0
43	35.008738999323036	38.0	34.0	40.0	24.0	41.0
44	34.818142654932615	38.0	34.0	40.0	24.0	41.0
45	34.75032309680596	38.0	34.0	40.0	23.0	41.0
46	34.661308388208504	38.0	34.0	40.0	23.0	41.0
47	34.575284632900484	37.0	34.0	40.0	23.0	41.0
48	34.48816542556465	37.0	34.0	40.0	23.0	41.0
49	34.36119453504831	37.0	33.0	40.0	23.0	41.0
50	34.19559665210167	37.0	33.0	40.0	23.0	41.0
51	32.47997415225552	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	21.0
10	18.0
11	22.0
12	10.0
13	16.0
14	6.0
15	30.0
16	41.0
17	80.0
18	177.0
19	320.0
20	547.0
21	776.0
22	1324.0
23	1869.0
24	2809.0
25	3854.0
26	5208.0
27	5876.0
28	5841.0
29	6491.0
30	7501.0
31	9289.0
32	12179.0
33	16737.0
34	25951.0
35	29077.0
36	31444.0
37	46615.0
38	61901.0
39	48937.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.36279155640347	19.716597944488893	23.233429749523047	16.68718074958459
2	35.09815988676227	25.024616899501506	23.600529263339283	16.276693950396947
3	32.063203889470124	23.266354852606312	29.78121730568035	14.889223952243217
4	30.064619361191458	24.072250600036927	28.210659117484155	17.652470921287463
5	26.074835374484586	28.52606314234722	26.41731798879931	18.981783494368884
6	25.061234537510003	34.0457874330728	26.73579912610007	14.157178903317128
7	64.11533017416457	19.4310419102714	12.652470921287465	3.8011569942765706
8	74.34088251584713	7.597698319896609	13.039263954704905	5.022155209551357
9	67.36229921841344	9.290417871869037	15.598498369130409	7.7487845405871125
10	38.808234352883254	25.563111576096993	22.590313250046158	13.038340820973598
11	30.339097790633268	24.87660779124869	27.332451227767862	17.451843190350175
12	29.40180934211336	22.302603237122284	31.162225367714935	17.13336205304942
13	23.442981106529633	26.178534063634686	31.691796418241125	18.68668841159456
14	18.689150101544712	29.532278909471355	31.247153670995136	20.531417317988797
15	18.45959751369315	25.748661456089607	36.81826573943012	18.973475290787125
16	20.24309188257739	25.65419410425257	33.730998830697274	20.37171518247277
17	20.352329374115328	25.352944796602866	31.112376146224385	23.182349683057417
18	22.301987814634746	25.76589328574066	31.74256877346298	20.18955012616161
19	24.294725829281802	25.716967197981415	29.269493507292754	20.71881346544403
20	24.691981044987386	24.950458489753217	31.57517385685273	18.78238660840667
21	23.559603667918026	26.79426426241615	31.26223152193981	18.383900547726014
22	22.1404394116561	24.630746507477383	30.356329620284324	22.87248446058219
23	21.057911256077297	27.163517754938766	30.92559542125669	20.852975567727245
24	22.02258600529263	23.650993907317375	32.581697335220625	21.744722752169366
25	20.497569081174227	27.885100621576715	30.193858083574376	21.42347221367469
26	19.706135762200752	28.50267708782079	29.364268570373564	22.426918579604898
27	21.2228444827374	28.28912548464521	30.058157425072313	20.42987260754508
28	18.364514739368577	27.106898886085297	34.37042279524894	20.158163579297188
29	20.132931257308144	26.477629392578006	32.67124130715736	20.71819804295649
30	21.6742568773463	27.197981414240875	31.33946704412579	19.788294664287033
31	23.30266477937104	26.010831435780663	30.290171702874026	20.396332081974275
32	23.0906517324143	26.98135269862761	30.500030771124376	19.427964797833713
33	23.32451227767863	26.78564834759062	29.247646008985168	20.642193365745584
34	20.910825281555788	27.339836297618312	31.297926026217	20.451412394608898
35	23.08880546495169	25.428641762570003	29.512585389870143	21.96996738260816
36	24.29749523047572	27.670010462182287	28.603914087020737	19.42858022032125
37	22.593082651240078	28.06018831928119	30.880054157178904	18.466674872299834
38	21.621330543418058	27.53400209243646	29.574435349867684	21.270232014277802
39	22.87740784048249	25.132315834820606	31.236999199950766	20.753277124746138
40	24.793833466674872	24.815680964982462	30.665887131515785	19.72459843682688
41	20.685272939873222	26.833343590374792	30.07662009969844	22.404763370053544
42	22.999569204258723	25.37294602744784	29.93291894885839	21.69456581943504
43	24.048864545510494	24.898147578312514	29.707366607175828	21.34562126900117
44	22.38691611791495	25.45356637331528	29.53350975444643	22.626007754323343
45	20.902824789217796	26.844728906394238	28.387593082651243	23.864853221736723
46	23.351590867130284	26.90319404271032	28.792541079451045	20.952674010708353
47	22.347221367468766	25.18893470367407	32.09982152747861	20.364022401378545
48	22.46384392885716	24.96522862945412	30.768970398178347	21.80195704351037
49	21.795495107391226	24.085174472275217	31.955197242907257	22.164133177426304
50	20.29386423779925	25.09262108437442	31.630869591974893	22.982645085851434
51	20.24832297372146	25.588959320573572	29.507969721213613	24.654747984491355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	158.0
1	480.5
2	803.0
3	3397.5
4	5992.0
5	4212.5
6	2433.0
7	2232.0
8	2031.0
9	1948.5
10	1866.0
11	1789.5
12	1713.0
13	1714.5
14	1716.0
15	1583.5
16	1451.0
17	1416.5
18	1382.0
19	1362.5
20	1343.0
21	1531.0
22	1719.0
23	1844.5
24	1970.0
25	2280.5
26	2805.5
27	3020.0
28	4072.0
29	5124.0
30	5636.0
31	6148.0
32	7059.0
33	7970.0
34	8961.0
35	9952.0
36	10025.5
37	10099.0
38	11003.5
39	11908.0
40	13655.0
41	15402.0
42	17935.0
43	20468.0
44	21541.5
45	22615.0
46	26347.0
47	30079.0
48	29793.0
49	29507.0
50	27838.5
51	26170.0
52	23126.0
53	20082.0
54	17745.0
55	15408.0
56	14162.5
57	12917.0
58	12442.5
59	11968.0
60	11449.0
61	10930.0
62	10030.5
63	9131.0
64	7879.0
65	6627.0
66	5407.5
67	4188.0
68	3677.0
69	3166.0
70	2501.0
71	1836.0
72	1490.0
73	1144.0
74	989.0
75	639.0
76	444.0
77	367.5
78	291.0
79	236.0
80	181.0
81	142.5
82	104.0
83	74.5
84	45.0
85	36.5
86	28.0
87	19.5
88	11.0
89	8.5
90	6.0
91	7.0
92	8.0
93	4.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	324980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.900348036707577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.34517331654597	23.39736555610454
2	9.387459617541182	5.98926457960203
3	3.8112554843191147	3.6474112921977073
4	2.1748583783000113	2.7751495679327913
5	1.4804609940672024	2.3613610482756915
6	1.1040668448114486	2.1132069963164897
7	0.8275470649430889	1.847932757190827
8	0.6378796620838791	1.6278866580810532
9	0.5480374100928865	1.5734325707189027
>10	6.46564346069744	45.979832532246554
>50	0.16570894153777702	3.3051318440102486
>100	0.04392285197386861	2.4835459342360466
>500	0.004991233178848706	1.1664609253887215
>1k	0.002994739907309223	1.7320177376984047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3400	1.0462182288140809	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	1028	0.316327158594375	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	1011	0.3110960674503046	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	939	0.28894085789894763	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	809	0.2489383962089975	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	724	0.22278294048864544	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	649	0.1997045972059819	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	542	0.16677949412271525	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	471	0.14493199581512708	Illumina Single End Adapter 2 (95% over 21bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	464	0.14277801710874516	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	391	0.12031509631361931	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16616407163517755	0.0
2	0.0	0.0	0.0	0.9139023939934765	0.0
3	0.0	0.0	0.0	1.160686811496092	0.0
4	0.0	0.0	0.0	1.5096313619299648	0.0
5	0.0	0.0	0.0	2.569696596713644	0.0
6	0.0	0.0	0.0	3.049110714505508	0.0
7	0.0	0.0	0.0	3.544218105729583	0.0
8	0.0	0.0	0.0	4.33534371345929	0.0
9	0.0	0.0	0.0	4.59412886946889	0.0
10	0.0	0.0	0.0	5.572650624653825	0.0
11	0.0	0.0	0.0	6.490860976060065	0.0
12	0.0	0.0	0.0	7.624776909348268	0.0
13	0.0	0.0	0.0	8.026340082466612	0.0
14	0.0	0.0	0.0	8.238660840667118	0.0
15	0.0	0.0	0.0	8.556526555480337	0.0
16	0.0	0.0	0.0	9.16702566311773	0.0
17	0.0	0.0	0.0	9.79721829035633	0.0
18	0.0	0.0	0.0	10.522185980675735	0.0
19	0.0	0.0	0.0	10.944058095882824	0.0
20	0.0	0.0	0.0	11.357006585020617	0.0
21	0.0	0.0	0.0	11.855806511169918	0.0
22	0.0	0.0	0.0	12.383223582989723	0.0
23	0.0	0.0	0.0	12.882331220382794	0.0
24	0.0	0.0	0.0	13.279894147332143	0.0
25	0.0	0.0	0.0	13.672841405624961	0.0
26	0.0	0.0	0.0	13.998399901532402	0.0
27	0.0	0.0	0.0	14.3510369868915	0.0
28	0.0	0.0	0.0	14.715059388270047	0.0
29	0.0	0.0	0.0	15.078158655917287	0.0
30	0.0	0.0	0.0	15.502492461074528	0.0
31	0.0	0.0	0.0	15.880361868422671	0.0
32	0.0	0.0	0.0	16.287156132685087	0.0
33	0.0	0.0	0.0	16.655178780232628	0.0
34	0.0	0.0	0.0	17.026893962705397	0.0
35	0.0	0.0	0.0	17.411533017416456	0.0
36	0.0	0.0	0.0	17.8081728106345	0.0
37	0.0	0.0	0.0	18.161733029724907	0.0
38	0.0	0.0	0.0	18.514985537571544	0.0
39	0.0	0.0	0.0	18.87069973536833	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACC	25	3.8846094E-5	45.000004	27
GCCTCGA	25	3.8846094E-5	45.000004	14
CTCGATC	25	3.8846094E-5	45.000004	16
TAGATCA	25	3.8846094E-5	45.000004	31
GAACCCG	25	3.8846094E-5	45.000004	1
TCGGTAC	25	3.8846094E-5	45.000004	22
CGGGTAA	20	7.02581E-4	45.0	28
CCCTCGT	85	0.0	45.0	14
ATCTAGA	20	7.02581E-4	45.0	21
TTGTCGG	20	7.02581E-4	45.0	29
GGCCGAT	45	3.8380676E-10	45.0	8
TCGATAG	20	7.02581E-4	45.0	1
TTATCCG	20	7.02581E-4	45.0	1
TCTACAC	20	7.02581E-4	45.0	17
CTACGAA	75	0.0	45.0	11
ATTGGCG	20	7.02581E-4	45.0	20
GCTACGA	75	0.0	45.0	10
TATACGG	35	1.2083365E-7	45.0	2
ACTCACG	20	7.02581E-4	45.0	24
CGAAGGC	20	7.02581E-4	45.0	4
>>END_MODULE