##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936632.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	917702
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11949303804503	31.0	31.0	33.0	28.0	34.0
2	31.216723947425198	31.0	31.0	34.0	28.0	34.0
3	30.826038300014602	31.0	30.0	34.0	27.0	34.0
4	34.7725950254004	35.0	35.0	37.0	32.0	37.0
5	35.11121039291622	35.0	35.0	37.0	33.0	37.0
6	35.117170933483855	36.0	35.0	37.0	32.0	37.0
7	31.170775480493667	35.0	35.0	37.0	0.0	37.0
8	33.14480626608638	36.0	35.0	37.0	17.0	37.0
9	36.43419105548424	39.0	35.0	39.0	32.0	39.0
10	36.24110332112167	37.0	35.0	39.0	32.0	39.0
11	36.49885583773382	38.0	35.0	39.0	32.0	39.0
12	36.60377551754273	39.0	35.0	39.0	32.0	39.0
13	36.60787597716906	39.0	35.0	39.0	32.0	39.0
14	37.677178430470896	40.0	37.0	41.0	33.0	41.0
15	37.83326068811008	40.0	37.0	41.0	33.0	41.0
16	37.7589162930886	40.0	37.0	41.0	33.0	41.0
17	37.64210931217323	39.0	36.0	41.0	33.0	41.0
18	37.469942312428216	39.0	36.0	40.0	32.0	41.0
19	37.16932293925479	39.0	36.0	40.0	32.0	41.0
20	37.02627868305834	39.0	35.0	40.0	32.0	41.0
21	37.01015907124535	39.0	35.0	40.0	32.0	41.0
22	37.07954325042334	39.0	35.0	40.0	32.0	41.0
23	37.13209407846992	39.0	35.0	40.0	32.0	41.0
24	37.04813545137746	39.0	35.0	40.0	32.0	41.0
25	36.70065446081625	38.0	35.0	40.0	31.0	41.0
26	36.696520221161116	38.0	35.0	40.0	31.0	41.0
27	36.72103144593779	38.0	35.0	40.0	31.0	41.0
28	36.68868979254704	38.0	35.0	40.0	31.0	41.0
29	36.64037345456368	39.0	35.0	40.0	31.0	41.0
30	36.33794630500969	38.0	35.0	40.0	30.0	41.0
31	36.21497501367546	38.0	35.0	40.0	30.0	41.0
32	35.90415080276604	38.0	35.0	40.0	29.0	41.0
33	35.56673844014724	38.0	35.0	40.0	27.0	41.0
34	35.29253613918244	38.0	35.0	40.0	25.0	41.0
35	35.101479565261926	38.0	34.0	40.0	23.0	41.0
36	34.97375509697048	38.0	34.0	40.0	23.0	41.0
37	34.876499124988285	38.0	34.0	40.0	23.0	41.0
38	34.7298349573173	38.0	34.0	40.0	22.0	41.0
39	34.62956493502248	38.0	34.0	40.0	21.0	41.0
40	34.49157569668585	38.0	34.0	40.0	21.0	41.0
41	34.29534424028715	38.0	33.0	40.0	19.0	41.0
42	34.30078609396079	38.0	34.0	40.0	19.0	41.0
43	34.25052468012492	38.0	33.0	40.0	19.0	41.0
44	34.033553375714554	38.0	33.0	40.0	18.0	41.0
45	33.92991733700046	38.0	33.0	40.0	18.0	41.0
46	33.83737640323329	37.0	33.0	40.0	18.0	41.0
47	33.75676853706323	37.0	33.0	40.0	18.0	41.0
48	33.68435178304068	37.0	33.0	40.0	18.0	41.0
49	33.59433781336425	37.0	33.0	40.0	18.0	41.0
50	33.45700456139357	37.0	33.0	40.0	18.0	41.0
51	31.934481999603356	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	45.0
9	74.0
10	86.0
11	92.0
12	48.0
13	43.0
14	57.0
15	107.0
16	196.0
17	356.0
18	652.0
19	1251.0
20	1987.0
21	3062.0
22	4653.0
23	6965.0
24	10791.0
25	15257.0
26	19986.0
27	21082.0
28	20533.0
29	20193.0
30	21816.0
31	26389.0
32	33125.0
33	44576.0
34	63961.0
35	74228.0
36	86304.0
37	128832.0
38	170780.0
39	140157.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.83515345940186	19.64602888519367	21.34145942800604	19.177358227398436
2	39.931807928935534	21.957018727212102	22.36553914015661	15.745634203695753
3	30.623884441790473	22.096715491521213	32.41313629042979	14.866263776258522
4	26.481145295531665	23.217231737535716	32.425122752265985	17.876500214666635
5	23.859924027625524	26.900344556293877	31.22920076451833	18.010530651562274
6	24.345811603330926	31.630856203865743	31.196837317560604	12.826494875242725
7	63.75664431373147	16.808506465061644	15.892195941601958	3.5426532796049264
8	74.70268126254493	5.385735238672249	16.168756306513444	3.742827192269386
9	68.72655829452262	6.8333729249800035	18.06185450178816	6.37821427870921
10	36.37967444769653	24.75683827647755	25.20524091698612	13.658246358839799
11	26.627924969107617	24.302115501546254	32.12044868595688	16.94951084338925
12	23.96050133921469	22.456636250111693	35.418033304929054	18.164829105744566
13	22.633164142608383	22.57519325445515	36.86501718422756	17.926625418708905
14	19.301472591320493	24.685573312469625	36.193230482226255	19.819723613983626
15	18.469394204218798	24.88683690348283	37.9341006121813	18.709668280117075
16	22.65168867453705	25.561129865686244	34.07620338628444	17.710978073492267
17	22.77460439227549	24.628692102665134	33.66746503767018	18.929238467389194
18	23.243384017905594	24.015856999330936	34.34993058748918	18.390828395274283
19	21.92302076273126	26.224090173062713	32.60361206579042	19.249276998415606
20	22.994283547382484	25.637516317933272	33.67367620425803	17.694523930426218
21	22.94285072932172	25.76468178123182	34.35810317510477	16.934364314341693
22	22.578680225171134	22.795308280901644	34.6413105779436	19.98470091598362
23	20.760660868124948	24.643729663877817	35.599682685664845	18.995926782332393
24	21.02044018646576	24.379809567811773	35.045472277493126	19.55427796822934
25	21.097371477887158	26.65821802720273	33.09483906540468	19.14957142950544
26	20.831380992958497	25.7574899041301	32.63891764428976	20.772211458621644
27	20.624995913706194	25.28119149789365	33.732409867255384	20.36140272114477
28	19.328278678699622	26.141492554227842	34.029347217288404	20.500881549784133
29	20.370664987109105	26.70267690383153	33.12349760597667	19.8031605030827
30	21.141067579671834	25.948510518665103	33.76324776452487	19.1471741371382
31	22.73886294243665	25.660726466761542	32.449313611608126	19.15109697919368
32	22.642644344242466	26.517431584544877	31.869822665745524	18.970101405467137
33	21.76817746937459	26.312027215806438	31.704082588901407	20.215712725917566
34	21.485296970040384	26.533340888436552	33.03305430303083	18.948307838492234
35	21.53367868872466	28.073710202222507	31.11151550285387	19.28109560619896
36	22.665745525235863	28.158269242085122	29.520258210181517	19.655727022497498
37	21.482136902829023	28.207304767778645	30.28543034667027	20.02512798272206
38	22.200561838156613	27.447363087363875	30.313326112398144	20.038748962081375
39	22.210368943295318	26.59000416257129	29.98162802304016	21.21799887109323
40	23.74888580388841	25.5933843448091	31.19629247838623	19.46143737291626
41	20.748238534949255	26.44899978424369	30.732852276664975	22.069909404142084
42	21.33633793976694	26.742995002735093	30.891618412077122	21.029048645420843
43	21.752813004657284	26.320090835587152	30.184635099411356	21.742461060344205
44	21.94437845836666	26.000161272395616	30.347433044713863	21.708027224523864
45	21.167328827876588	25.96954131079588	29.897722790186794	22.96540707114074
46	21.449555520201546	27.017484978783962	29.707029079156417	21.825930421858075
47	20.4920551551593	26.6929787665277	31.68261592543113	21.132350152881873
48	20.659647685196283	26.94186130138106	31.369442368001817	21.029048645420843
49	21.11338974961371	26.07600288546827	31.35211648225677	21.458490882661255
50	19.7698163456111	26.801728665732448	32.14463954529902	21.28381544335743
51	19.751182845847563	25.892501051539607	30.967677960819522	23.388638141793304
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	301.0
1	798.5
2	1296.0
3	15341.0
4	29386.0
5	19769.0
6	10152.0
7	9440.5
8	8729.0
9	8542.5
10	8356.0
11	8151.5
12	7947.0
13	7548.5
14	7150.0
15	6821.5
16	6493.0
17	6191.0
18	5889.0
19	5543.5
20	5198.0
21	5329.0
22	5460.0
23	5609.0
24	5758.0
25	6525.0
26	8710.5
27	10129.0
28	11387.5
29	12646.0
30	14524.5
31	16403.0
32	19281.0
33	22159.0
34	25779.5
35	29400.0
36	31327.5
37	33255.0
38	35767.0
39	38279.0
40	41382.0
41	44485.0
42	46003.5
43	47522.0
44	50998.5
45	54475.0
46	64645.5
47	74816.0
48	72018.0
49	69220.0
50	67357.0
51	65494.0
52	61278.5
53	57063.0
54	52641.5
55	48220.0
56	44966.5
57	41713.0
58	39140.5
59	36568.0
60	33291.5
61	30015.0
62	26986.0
63	23957.0
64	20752.0
65	17547.0
66	14903.0
67	12259.0
68	10520.0
69	8781.0
70	7412.0
71	6043.0
72	4747.5
73	3452.0
74	2736.5
75	1604.5
76	1188.0
77	891.5
78	595.0
79	460.0
80	325.0
81	228.5
82	132.0
83	92.5
84	53.0
85	46.5
86	40.0
87	28.0
88	16.0
89	13.5
90	11.0
91	9.0
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	917702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.02581791263051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43858061810118	27.925620943459517
2	8.983015434635764	6.8317301844761795
3	4.248993068635485	4.847143102198863
4	2.4921185129368633	3.7905937915853074
5	1.6400948824401056	3.11829746795523
6	1.1082301237617225	2.528481413487314
7	0.8870237287508711	2.361086195556423
8	0.7255338054425966	2.2071213100174454
9	0.6284353496575256	2.150709135834362
>10	5.785642559997463	38.24762827522127
>50	0.04005052749367804	1.0130842624534417
>100	0.018332787716148224	1.3830845869797537
>500	0.0016922573273550315	0.4103351139346327
>1k	0.001974300215247537	1.317233630252263
>5k	0.0	0.0
>10k+	2.8204288789250527E-4	1.867850586587967
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17416	1.8977838121743225	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	3567	0.3886882669973477	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	2200	0.23972923672390384	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	2051	0.22349302932760307	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1238	0.13490217957463316	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	1143	0.1245502352615555	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1043	0.11365345177410532	No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1040	0.11332654826948182	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTT	922	0.10046834375429062	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1793566974900348E-4	0.0	0.0	0.04838171868427878	0.0
2	2.1793566974900348E-4	0.0	0.0	0.26566358142403523	0.0
3	2.1793566974900348E-4	0.0	0.0	0.42758978404754483	0.0
4	2.1793566974900348E-4	0.0	0.0	0.748826961257576	0.0
5	2.1793566974900348E-4	0.0	0.0	1.625146289318319	0.0
6	2.1793566974900348E-4	0.0	0.0	2.349673423398881	0.0
7	2.1793566974900348E-4	0.0	0.0	2.8208503413962265	0.0
8	2.1793566974900348E-4	0.0	0.0	3.462017081797795	0.0
9	2.1793566974900348E-4	0.0	0.0	3.863127681970836	0.0
10	2.1793566974900348E-4	0.0	0.0	4.64529880070001	0.0
11	2.1793566974900348E-4	0.0	0.0	5.129007019707923	0.0
12	2.1793566974900348E-4	0.0	0.0	5.740534509023626	0.0
13	2.1793566974900348E-4	0.0	0.0	5.979174067398786	0.0
14	2.1793566974900348E-4	0.0	0.0	6.141645109196667	0.0
15	2.1793566974900348E-4	0.0	0.0	6.298122920076452	0.0
16	2.1793566974900348E-4	0.0	0.0	6.504507999328758	0.0
17	2.1793566974900348E-4	0.0	0.0	6.729199674839981	0.0
18	2.1793566974900348E-4	0.0	0.0	6.9864727329786795	0.0
19	2.1793566974900348E-4	0.0	0.0	7.163327528979996	0.0
20	2.1793566974900348E-4	0.0	0.0	7.339528517972065	0.0
21	2.1793566974900348E-4	0.0	0.0	7.538176880948281	0.0
22	2.1793566974900348E-4	0.0	0.0	7.761778878110759	0.0
23	2.1793566974900348E-4	0.0	0.0	7.98232977589675	0.0
24	2.1793566974900348E-4	0.0	0.0	8.17160690507376	0.0
25	2.1793566974900348E-4	0.0	0.0	8.354890803332673	0.0
26	2.1793566974900348E-4	0.0	0.0	8.533271149022232	0.0
27	2.1793566974900348E-4	0.0	0.0	8.723855892217735	0.0
28	2.1793566974900348E-4	0.0	0.0	8.917927606129222	0.0
29	2.1793566974900348E-4	0.0	0.0	9.132485272997117	0.0
30	2.1793566974900348E-4	0.0	0.0	9.412750544294335	0.0
31	2.1793566974900348E-4	0.0	0.0	9.652370813183364	0.0
32	2.1793566974900348E-4	0.0	0.0	9.940372800756673	0.0
33	2.1793566974900348E-4	0.0	0.0	10.176179195425094	0.0
34	2.1793566974900348E-4	0.0	0.0	10.412421461433015	0.0
35	2.1793566974900348E-4	0.0	0.0	10.665553741846482	0.0
36	2.1793566974900348E-4	0.0	0.0	10.903321557542645	0.0
37	2.1793566974900348E-4	0.0	0.0	11.160812551351093	0.0
38	2.1793566974900348E-4	0.0	0.0	11.456987126539987	0.0
39	2.1793566974900348E-4	0.0	0.0	11.893839176551866	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCTAG	25	3.890189E-5	45.0	2
TTACGCG	20	7.032544E-4	45.0	1
CGTTTTT	8450	0.0	44.068047	1
CGTTTTA	475	0.0	39.789474	1
CACAACG	215	0.0	39.767445	12
CGGTCTA	65	9.094947E-12	38.07692	31
ACACGAC	95	0.0	37.894737	26
GCGGTAT	30	1.13974245E-4	37.500004	1
CGCATAG	30	1.13974245E-4	37.500004	2
TAACGCG	30	1.13974245E-4	37.500004	1
GCGCATA	30	1.13974245E-4	37.500004	1
TCTACGG	30	1.13974245E-4	37.500004	2
ACAACGA	230	0.0	37.173912	13
GTTTTTT	10290	0.0	37.12828	2
ATTAGCG	55	2.748493E-9	36.81818	1
AGCGACT	215	0.0	36.62791	19
TACGGCT	295	0.0	36.61017	7
CGTTGAT	150	0.0	36.000004	25
GCTATCG	25	0.0021068878	36.0	1
CACGACC	100	0.0	36.0	27
>>END_MODULE