##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936631.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	192655
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.985938594897615	31.0	30.0	33.0	28.0	34.0
2	31.037574939658978	31.0	31.0	33.0	28.0	34.0
3	30.639277464898395	31.0	30.0	34.0	27.0	34.0
4	34.586016454283566	35.0	35.0	37.0	32.0	37.0
5	34.99992214061405	35.0	35.0	37.0	32.0	37.0
6	35.05667125171939	35.0	35.0	37.0	32.0	37.0
7	31.27240922893255	35.0	35.0	37.0	0.0	37.0
8	33.25480781708235	36.0	35.0	37.0	17.0	37.0
9	36.42198749059199	39.0	35.0	39.0	32.0	39.0
10	36.18015104720874	37.0	35.0	39.0	32.0	39.0
11	36.47914147050427	38.0	35.0	39.0	32.0	39.0
12	36.420975318574655	38.0	35.0	39.0	32.0	39.0
13	36.13940982585451	38.0	35.0	39.0	32.0	39.0
14	37.08896732501103	39.0	36.0	40.0	32.0	41.0
15	37.36042147880927	39.0	36.0	41.0	32.0	41.0
16	37.42044068412447	39.0	36.0	40.0	32.0	41.0
17	37.356284550102515	39.0	35.0	40.0	32.0	41.0
18	37.193589577223534	39.0	36.0	40.0	32.0	41.0
19	36.85269523241027	38.0	35.0	40.0	32.0	41.0
20	36.684160805585115	38.0	35.0	40.0	32.0	41.0
21	36.66512159040772	38.0	35.0	40.0	32.0	41.0
22	36.741719654304326	38.0	35.0	40.0	32.0	41.0
23	36.81016843580494	38.0	35.0	40.0	32.0	41.0
24	36.70190236433002	38.0	35.0	40.0	32.0	41.0
25	36.40209182216916	38.0	35.0	40.0	31.0	41.0
26	36.424042978381046	38.0	35.0	40.0	31.0	41.0
27	36.4672341750798	38.0	35.0	40.0	31.0	41.0
28	36.44753575043472	38.0	35.0	40.0	31.0	41.0
29	36.42999143546754	38.0	35.0	40.0	31.0	41.0
30	36.14860761464794	38.0	35.0	40.0	31.0	41.0
31	35.93933196646856	38.0	35.0	40.0	30.0	41.0
32	35.515143650567076	38.0	35.0	40.0	29.0	41.0
33	35.12665645843607	38.0	34.0	40.0	25.0	41.0
34	34.82464509096572	38.0	34.0	40.0	23.0	41.0
35	34.591030598738676	38.0	34.0	40.0	23.0	41.0
36	34.48121253017051	38.0	34.0	40.0	21.0	41.0
37	34.33943577898316	38.0	34.0	40.0	21.0	41.0
38	34.22249098128779	38.0	34.0	40.0	20.0	41.0
39	34.18010433157717	38.0	34.0	40.0	19.0	41.0
40	34.017995899405676	38.0	33.0	40.0	18.0	41.0
41	33.8817471646207	37.0	33.0	40.0	18.0	41.0
42	33.86978796293893	37.0	33.0	40.0	18.0	41.0
43	33.78318237263502	37.0	33.0	40.0	17.0	41.0
44	33.57031481145052	37.0	33.0	40.0	15.0	41.0
45	33.50540084607199	37.0	33.0	40.0	15.0	41.0
46	33.38329137577535	37.0	33.0	40.0	15.0	41.0
47	33.324211673717265	37.0	33.0	40.0	15.0	41.0
48	33.202776984765514	37.0	33.0	40.0	15.0	41.0
49	33.111380446912875	37.0	33.0	40.0	14.0	41.0
50	32.89117853157198	36.0	32.0	40.0	12.0	41.0
51	31.28729075290026	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	4.0
9	11.0
10	11.0
11	9.0
12	7.0
13	7.0
14	5.0
15	5.0
16	22.0
17	45.0
18	115.0
19	226.0
20	377.0
21	613.0
22	1035.0
23	1580.0
24	2594.0
25	3782.0
26	4880.0
27	4661.0
28	4300.0
29	4287.0
30	4817.0
31	5895.0
32	7621.0
33	10632.0
34	16634.0
35	18891.0
36	17548.0
37	25867.0
38	33260.0
39	22913.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.94093067919338	20.229425657262983	24.41514624587994	19.414497417663696
2	37.579092159559835	23.547792686408346	24.804962238197813	14.068152915834004
3	27.966053307726245	22.64047130881628	34.36401858244012	15.029456801017362
4	25.570579533362746	21.877968389089304	36.3572188627339	16.194233214814048
5	22.503957852119072	26.96737691728738	34.27422075731229	16.254444473281254
6	21.64023773065843	31.32490721756508	33.77280631180089	13.262048739975604
7	60.55020632737277	16.44338324985077	19.02883392592977	3.977576496846695
8	70.81934027146973	5.591342036282474	18.29124600970647	5.29807168254133
9	67.21289351431315	5.223845734603307	20.19257221458047	7.370688536503075
10	37.40364900988814	22.771275077210557	26.774285640133915	13.050790272767381
11	28.511069009369077	23.84106304014949	32.2358620331681	15.412005917313332
12	27.46100542420389	20.19828190288339	35.875528794996235	16.465183877916484
13	20.582907269471335	27.81292984869326	37.307622433884404	14.296540447951001
14	16.00529443824453	32.890919000285486	34.96509304196621	16.13869351950378
15	12.93763463185487	25.578365471957643	46.615452492798006	14.86854740338948
16	15.943006929485348	23.96356180737588	41.526043964599936	18.56738729853884
17	15.39539591497755	22.70431600529444	36.4937323194311	25.406555760296907
18	18.655627935947678	23.65316238872596	38.55804417222496	19.1331655031014
19	18.547143858192104	26.46907684721393	35.25732527056137	19.7264540240326
20	21.306999558796814	25.09667540421998	36.47037450364641	17.125950533336795
21	18.967584542316576	26.55576029690379	36.516052010069814	17.960603150709815
22	16.49269419428512	23.674963016791676	35.81220316109107	24.020139627832133
23	14.64275518413745	26.36422620746931	36.62972671355532	22.36329189483792
24	18.620850743557135	23.42269860631699	37.46853183151229	20.487918818613586
25	14.285640133918143	26.454543095170123	37.312813059614335	21.9470037112974
26	15.044509615634164	27.298538838857024	37.94191689808206	19.715034647426748
27	16.505151696036958	27.46619604993382	38.13137473722457	17.897277516804653
28	13.283330305468324	25.08888946562508	42.61036567958267	19.01741454932392
29	14.731514884119282	21.608055851132853	41.99164309257481	21.668786172173053
30	16.430406685525938	24.65547221717578	40.1292465806753	18.784874516622978
31	21.009057641898732	24.646648153434896	35.27289714775116	19.071397056915213
32	19.2774648983935	27.261166333601516	33.31499312242091	20.146375645584076
33	17.928940333757236	27.129324440061247	32.55560457813189	22.386130648049622
34	18.012509408009137	24.84337286859931	34.86647115309751	22.27764657029405
35	15.087072746619604	26.898341595079287	34.07957229243985	23.935013365861256
36	19.91954530118606	25.174534790168956	36.264306662168124	18.64161324647686
37	17.68290467415847	25.950014274220756	36.8446186187745	19.52246243284628
38	15.896810360488958	29.317173185227478	30.56344242298409	24.222574031299473
39	19.551529936933896	25.013625392541073	32.5545664529859	22.880278217539125
40	21.010095767044717	25.54306921699411	31.996574187018247	21.45026082894293
41	18.020295346604033	26.933637850042825	31.034232176688896	24.011834626664243
42	20.53826788819392	25.56175546962186	32.97500713711038	20.924969505073836
43	21.527082089745917	24.44421374996756	32.13775920687239	21.890944953414134
44	17.81682281799071	25.53216890296125	32.80994523889855	23.84106304014949
45	17.999013781111316	25.162596350990114	30.760686200721498	26.077703667177076
46	21.843710259271756	25.518154213490433	30.99270717084945	21.645428356388365
47	17.78983156419506	25.0416547714827	34.972878980561106	22.195634683761128
48	19.433702732864447	24.668967844073602	32.43673924891646	23.460590174145494
49	18.67535231372142	21.621551478030675	36.025018816018274	23.678077392229632
50	18.09296410682308	23.40401235368924	34.423191715761334	24.07983182372635
51	17.90662064311853	24.130180893306687	29.756300121979706	28.20689834159508
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	160.0
1	199.5
2	239.0
3	3116.0
4	5993.0
5	4083.5
6	2174.0
7	2094.0
8	2014.0
9	2085.0
10	2156.0
11	2184.5
12	2213.0
13	2150.5
14	2088.0
15	1925.0
16	1762.0
17	1624.0
18	1486.0
19	1394.5
20	1303.0
21	1250.0
22	1197.0
23	1107.0
24	1017.0
25	1165.0
26	1502.5
27	1692.0
28	1825.5
29	1959.0
30	2269.5
31	2580.0
32	2957.0
33	3334.0
34	4072.5
35	4811.0
36	4949.0
37	5087.0
38	6367.5
39	7648.0
40	9408.0
41	11168.0
42	13749.5
43	16331.0
44	20632.0
45	24933.0
46	24499.5
47	24066.0
48	22428.5
49	20791.0
50	16875.0
51	12959.0
52	10893.5
53	8828.0
54	7610.5
55	6393.0
56	5404.5
57	4416.0
58	3984.5
59	3553.0
60	3254.5
61	2956.0
62	2372.5
63	1789.0
64	1366.0
65	943.0
66	758.5
67	574.0
68	455.5
69	337.0
70	249.0
71	161.0
72	128.0
73	95.0
74	83.0
75	52.5
76	34.0
77	25.5
78	17.0
79	12.0
80	7.0
81	4.0
82	1.0
83	2.0
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	192655.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.71705899146142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.20791340052259	32.208870779372454
2	10.039815851685953	8.376631802963848
3	3.7625979843225084	4.708935662194078
4	2.0057235286798556	3.3469154706599884
5	1.2081622495956204	2.520048791881861
6	0.8286674132138858	2.074174041680725
7	0.5835510762722409	1.704082427136591
8	0.39815851685952475	1.3288001868625263
9	0.33470200323503796	1.2566504892164752
>10	3.4502923976608186	31.733928525083698
>50	0.13562274480527559	3.576341127922971
>100	0.03732736095558044	2.714697256754302
>500	0.0024884907303720292	0.7137110378656146
>1k	0.0049769814607440585	3.736212400404869
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3781	1.962575588487192	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1191	0.6182035244348706	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC	1129	0.5860216449092938	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	1097	0.5694116425735123	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	861	0.4469128753471231	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	514	0.26679816251849164	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	485	0.25174534790168956	No Hit
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	315	0.16350471049284993	No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	302	0.15675689704393864	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	269	0.13962783213516392	No Hit
GGCAAAGGGAATAAATAAATAAGAAAAAAAGATAGAGAAAATGTATGTGAG	251	0.13028470582128673	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT	238	0.1235368923723755	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCC	217	0.11263657833951884	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	209	0.10848407775557344	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28756066543821857	0.0
2	0.0	0.0	0.0	1.682281799070878	0.0
3	0.0	0.0	0.0	2.159300303651605	0.0
4	0.0	0.0	0.0	2.984609794710752	0.0
5	0.0	0.0	0.0	5.112247281409774	0.0
6	0.0	0.0	0.0	6.008149282395993	0.0
7	0.0	0.0	0.0	7.1272481897692765	0.0
8	0.0	0.0	0.0	8.907632815135864	0.0
9	0.0	0.0	0.0	9.477044457709377	0.0
10	0.0	0.0	0.0	11.454153798240378	0.0
11	0.0	0.0	0.0	13.379875944045054	0.0
12	0.0	0.0	0.0	15.549557499156524	0.0
13	0.0	0.0	0.0	16.29129791596377	0.0
14	0.0	0.0	0.0	16.59183514572682	0.0
15	0.0	0.0	0.0	17.117645532168904	0.0
16	0.0	0.0	0.0	18.115802860034776	0.0
17	0.0	0.0	0.0	19.41138304222574	0.0
18	0.0	0.0	0.0	20.97116607407023	0.0
19	0.0	0.0	0.0	21.77467493706366	0.0
20	0.0	0.0	0.0	22.55949754742934	0.0
21	0.0	0.0	0.0	23.440865796371753	0.0
22	0.0	0.0	0.0	24.342477485660897	0.0
23	0.0	0.0	0.0	25.2015260439646	0.0
24	0.0	0.0	0.0	25.880978952012665	0.0
25	0.0	0.0	0.0	26.498663413874542	0.0
26	0.0	0.0	0.0	27.023954737743637	0.0
27	0.0	0.0	0.0	27.50875918091926	0.0
28	0.0	0.0	0.0	28.04495081882121	0.0
29	0.0	0.0	0.0	28.572837455555266	0.0
30	0.0	0.0	0.0	29.224780047234695	0.0
31	0.0	0.0	0.0	29.852845760556434	0.0
32	0.0	0.0	0.0	30.344917079753966	0.0
33	0.0	0.0	0.0	30.860346214736186	0.0
34	0.0	0.0	0.0	31.3840803508863	0.0
35	0.0	0.0	0.0	32.03550387999273	0.0
36	0.0	0.0	0.0	32.58207676935454	0.0
37	0.0	0.0	0.0	33.08349121486595	0.0
38	0.0	0.0	0.0	33.61864472762191	0.0
39	0.0	0.0	0.0	34.14082167605305	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCACAT	20	7.0186576E-4	45.0	33
CTTGTGT	35	1.2048986E-7	45.0	37
GCCCCGG	20	7.0186576E-4	45.0	14
ATTTACG	20	7.0186576E-4	45.0	1
ATGGGTA	20	7.0186576E-4	45.0	5
GTGCAAC	40	6.7648216E-9	45.0	2
CGGGTCC	20	7.0186576E-4	45.0	6
TGTCAAC	25	3.8786846E-5	45.0	21
GAAGCGA	25	3.8786846E-5	45.0	13
ATTAGTG	20	7.0186576E-4	45.0	29
AGTCATC	25	3.8786846E-5	45.0	40
CTTCCAA	20	7.0186576E-4	45.0	10
CCCTAGC	25	3.8786846E-5	45.0	21
AACCCGC	20	7.0186576E-4	45.0	25
GGGTCGT	20	7.0186576E-4	45.0	7
GCGCGAC	20	7.0186576E-4	45.0	9
AGCCCCG	20	7.0186576E-4	45.0	13
GCAACGG	40	6.7648216E-9	45.0	4
CATGAGC	20	7.0186576E-4	45.0	28
GACGTCC	20	7.0186576E-4	45.0	15
>>END_MODULE