##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936630.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	866273
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.131556680168952	31.0	31.0	33.0	28.0	34.0
2	31.194928157751654	31.0	31.0	34.0	28.0	34.0
3	30.850498630339395	31.0	30.0	34.0	27.0	34.0
4	34.81645855290422	35.0	35.0	37.0	32.0	37.0
5	35.10970675526075	35.0	35.0	37.0	33.0	37.0
6	35.099440938364694	36.0	35.0	37.0	32.0	37.0
7	30.978313995703434	35.0	35.0	37.0	0.0	37.0
8	32.99651610981758	35.0	35.0	37.0	17.0	37.0
9	36.247383907844295	39.0	35.0	39.0	32.0	39.0
10	36.16098620180936	37.0	35.0	39.0	32.0	39.0
11	36.413654817823016	38.0	35.0	39.0	32.0	39.0
12	36.49941300259849	38.0	35.0	39.0	32.0	39.0
13	36.4635259323562	39.0	35.0	39.0	32.0	39.0
14	37.5069164108774	39.0	36.0	41.0	32.0	41.0
15	37.66103179944429	40.0	36.0	41.0	32.0	41.0
16	37.61277449487633	39.0	36.0	41.0	33.0	41.0
17	37.487714611906405	39.0	36.0	41.0	32.0	41.0
18	37.31822762570229	39.0	36.0	40.0	32.0	41.0
19	37.06521038979629	39.0	36.0	40.0	32.0	41.0
20	36.919565771991046	39.0	35.0	40.0	32.0	41.0
21	36.95291322712355	39.0	35.0	40.0	32.0	41.0
22	37.0284159843375	39.0	35.0	40.0	32.0	41.0
23	37.09544335330779	39.0	35.0	40.0	32.0	41.0
24	37.01713893887955	39.0	35.0	40.0	32.0	41.0
25	36.676892850175406	38.0	35.0	40.0	31.0	41.0
26	36.65669944694109	38.0	35.0	40.0	31.0	41.0
27	36.68858546901497	38.0	35.0	40.0	31.0	41.0
28	36.60874227870429	38.0	35.0	40.0	31.0	41.0
29	36.50516176771064	38.0	35.0	40.0	31.0	41.0
30	36.23432220558646	38.0	35.0	40.0	30.0	41.0
31	36.15274630514861	38.0	35.0	40.0	30.0	41.0
32	35.904497773796486	38.0	35.0	40.0	29.0	41.0
33	35.607822245412244	38.0	35.0	40.0	27.0	41.0
34	35.39209810302295	38.0	35.0	40.0	25.0	41.0
35	35.22034739625961	38.0	34.0	40.0	25.0	41.0
36	35.10074537703472	38.0	34.0	40.0	24.0	41.0
37	35.046338740789565	38.0	34.0	40.0	24.0	41.0
38	34.87709532676189	38.0	34.0	40.0	23.0	41.0
39	34.77066236625175	38.0	34.0	40.0	23.0	41.0
40	34.648578450442294	38.0	34.0	40.0	23.0	41.0
41	34.46166624147353	38.0	34.0	40.0	22.0	41.0
42	34.45090866274258	38.0	34.0	40.0	22.0	41.0
43	34.41302453152759	38.0	34.0	40.0	21.0	41.0
44	34.25295720863977	38.0	33.0	40.0	21.0	41.0
45	34.1862992382309	38.0	33.0	40.0	21.0	41.0
46	34.088709910155345	37.0	33.0	40.0	20.0	41.0
47	34.0263669766921	37.0	33.0	40.0	20.0	41.0
48	33.95163995645714	37.0	33.0	40.0	20.0	41.0
49	33.83396573597469	37.0	33.0	40.0	20.0	41.0
50	33.69499337968516	37.0	33.0	40.0	20.0	41.0
51	32.11888169203011	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	41.0
9	89.0
10	95.0
11	65.0
12	66.0
13	55.0
14	61.0
15	100.0
16	203.0
17	359.0
18	680.0
19	1207.0
20	1989.0
21	3056.0
22	4508.0
23	6509.0
24	9123.0
25	13124.0
26	16689.0
27	18088.0
28	17952.0
29	19030.0
30	20759.0
31	25708.0
32	32710.0
33	44722.0
34	64152.0
35	73324.0
36	85019.0
37	123134.0
38	157700.0
39	125942.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91527728556702	19.83785711894518	21.523468929540687	17.723396665947106
2	38.35592244015455	23.94268319571313	21.963976714038182	15.737417650094137
3	30.656040301383054	23.48301286084179	30.363061067354057	15.497885770421101
4	27.490294629983854	23.557123447227372	31.312415370212392	17.64016655257638
5	25.312343799241116	26.399876251481924	29.537224408471698	18.750555540805266
6	25.48688461951371	32.327568791824284	29.2061509477959	12.979395640866102
7	63.83899763700358	17.544122926606278	14.837239530725302	3.779639905664842
8	74.11635823810738	6.572870215278555	15.165888813341752	4.144882733272306
9	67.39411247955321	7.374118782416167	17.444731626173272	7.787037111857348
10	38.52688471186335	23.03673322382205	24.152663190472285	14.28371887384231
11	28.926100663416726	24.42867317808589	29.53572372681591	17.109502431681467
12	25.71937483910961	21.999877636726527	33.746405578841774	18.534341945322087
13	24.557847237533664	21.538706620199406	35.54699269168034	18.356453450586592
14	20.152653955508253	25.113099450173326	34.953992563545214	19.780254030773207
15	19.525138149290118	24.101755451226115	37.58768886944416	18.785417530039606
16	23.505407648628086	24.652390181847984	33.204197752902374	18.638004416621552
17	24.017601841451828	24.22215629484008	32.070836791634974	19.68940507207312
18	24.058812868460635	24.08467076776028	32.6491764143636	19.207339949415484
19	23.90689771007523	24.882456223384544	31.268780165144243	19.941865901395982
20	24.449567284216407	25.73080310710365	31.599853625820035	18.219775982859908
21	24.603675746560263	25.157427277544148	32.73344546118833	17.505451514707257
22	23.277188599898647	22.6913455688911	33.741210911571756	20.2902549196385
23	22.06556131842964	24.193874217481095	33.52545906429036	20.215105399798908
24	22.514380570559165	24.07612842602736	32.64167300608469	20.767817997328784
25	22.493140153277317	26.371016988870714	30.739154977703333	20.396687880148637
26	21.57218336482841	25.43470707271264	31.140760476200917	21.852349086258027
27	21.85142558985447	24.793916005693355	32.46551606710587	20.889142337346307
28	20.891451078355207	25.51712912673026	32.64225019133691	20.949169603577626
29	21.829146239118614	26.58688427320256	31.916959203392004	19.667010284286825
30	23.132199664539932	26.300254076948026	31.568801059250372	18.998745199261666
31	24.091827864887858	25.839429371572244	30.50874262501544	19.56000013852446
32	25.45964147560873	25.916425884218945	29.613874609967066	19.01005803020526
33	24.12842140987887	25.87521485721014	29.386348183540296	20.610015549370694
34	23.87226659494178	25.910307720545372	30.636069691656093	19.581355992856757
35	22.471322550743242	27.43961776483857	29.25301839027651	20.836041294141687
36	23.459810013702377	27.625933164256534	29.422826291480863	19.491430530560226
37	23.328673524397043	27.461319930322198	28.96719625337509	20.24281029190567
38	22.97416634248095	26.83137994604472	28.9435316580339	21.250922053440426
39	24.05107858608083	25.437592998973763	29.07270571748167	21.43862269746373
40	24.04103556269213	25.29999203484352	30.205143182345516	20.45382922011883
41	22.132514807687645	25.803528448883895	29.973807333254065	22.09014941017439
42	21.63278781631195	26.143375125393497	31.056953177577967	21.166883880716586
43	22.372162124411126	25.136071423211853	30.601438576522643	21.89032787585438
44	22.694116058101777	25.15546484768658	29.641694939124267	22.508724155087368
45	22.26145799303453	24.782372300648873	29.135503472923663	23.820666233392938
46	22.72332163186432	26.183778093049188	29.09579312757064	21.99710714751585
47	20.872865713233587	26.02008835551841	31.842155994703745	21.264889936544254
48	21.465057782015602	25.664311366047425	30.60871111070067	22.26191974123631
49	21.875898244548775	25.049839946529556	31.624210843463896	21.45005096545777
50	21.16907718467504	25.33508489817875	31.370249332485255	22.125588584660953
51	21.298482118223703	24.75801508300501	30.097902162482264	23.84560063628902
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	336.0
1	793.5
2	1251.0
3	12745.5
4	24240.0
5	15954.5
6	7669.0
7	7092.0
8	6515.0
9	6467.5
10	6420.0
11	6284.0
12	6148.0
13	5926.0
14	5704.0
15	5411.0
16	5118.0
17	4909.5
18	4701.0
19	4559.0
20	4417.0
21	4601.5
22	4786.0
23	4973.5
24	5161.0
25	5765.0
26	7334.0
27	8299.0
28	9552.0
29	10805.0
30	12718.5
31	14632.0
32	16547.0
33	18462.0
34	21364.5
35	24267.0
36	25961.0
37	27655.0
38	30231.5
39	32808.0
40	34574.5
41	36341.0
42	39190.5
43	42040.0
44	46222.0
45	50404.0
46	64007.0
47	77610.0
48	70939.5
49	64269.0
50	63335.0
51	62401.0
52	56659.0
53	50917.0
54	48214.0
55	45511.0
56	43601.0
57	41691.0
58	40804.0
59	39917.0
60	37125.5
61	34334.0
62	31566.5
63	28799.0
64	25328.0
65	21857.0
66	18826.5
67	15796.0
68	13419.5
69	11043.0
70	9208.0
71	7373.0
72	5913.0
73	4453.0
74	3552.0
75	2093.0
76	1535.0
77	1128.0
78	721.0
79	543.5
80	366.0
81	306.0
82	246.0
83	168.5
84	91.0
85	74.0
86	57.0
87	45.5
88	34.0
89	25.5
90	17.0
91	17.5
92	18.0
93	13.0
94	8.0
95	7.0
96	6.0
97	5.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	866273.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.92566191611131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41970078310118	27.110710490988467
2	9.03159024955451	6.669948962397942
3	4.20093732672417	4.653671743721674
4	2.489481644113752	3.6770303014763734
5	1.569806495433888	2.898307196205364
6	1.1192434531373443	2.4797283211422325
7	0.8421259568724792	2.1767240861978427
8	0.6817905814444848	2.0140454806406396
9	0.594838509391774	1.9768325123235821
>10	5.98172077844463	39.40240186092634
>50	0.042175434147498705	1.0611455541044907
>100	0.021087659158354366	1.3758862348488479
>500	0.0030561824866390517	0.7675034680991586
>1k	0.001833709491983431	1.3929268205775496
>5k	3.0561824866390513E-4	0.6859117893113785
>10k+	3.0561824866390513E-4	1.6572251770381037
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14685	1.6951930857824267	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	6078	0.7016263926037173	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG	3500	0.4040296765569284	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC	2929	0.3381151207529266	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT	1919	0.2215236998036416	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT	1526	0.17615693897882076	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	1415	0.1633434263794439	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1054	0.12167065116885786	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT	954	0.1101269461243742	No Hit
ACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAAC	904	0.10435509360213234	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3087410088967335E-4	0.0	0.0	0.042480834563699894	0.0
2	2.3087410088967335E-4	0.0	0.0	0.3127189696550626	0.0
3	2.3087410088967335E-4	0.0	0.0	0.5059605920997191	0.0
4	2.3087410088967335E-4	0.0	0.0	0.93296224169517	0.0
5	2.3087410088967335E-4	0.0	0.0	2.129005521354123	0.0
6	2.3087410088967335E-4	0.0	0.0	2.927599036331503	0.0
7	2.3087410088967335E-4	0.0	0.0	3.4139353298555997	0.0
8	2.3087410088967335E-4	0.0	0.0	4.058420382489123	0.0
9	2.3087410088967335E-4	0.0	0.0	4.437169344998632	0.0
10	2.3087410088967335E-4	0.0	0.0	5.393796182034993	0.0
11	2.3087410088967335E-4	0.0	0.0	5.935080511570833	0.0
12	2.3087410088967335E-4	0.0	0.0	6.74267811648291	0.0
13	2.3087410088967335E-4	0.0	0.0	7.023767334316087	0.0
14	2.3087410088967335E-4	0.0	0.0	7.216200897407631	0.0
15	2.3087410088967335E-4	0.0	0.0	7.407249215893835	0.0
16	2.3087410088967335E-4	0.0	0.0	7.651398577584664	0.0
17	2.3087410088967335E-4	0.0	0.0	7.906629896118198	0.0
18	2.3087410088967335E-4	0.0	0.0	8.200763500651641	0.0
19	3.4631115133451E-4	0.0	0.0	8.392735315541406	0.0
20	3.4631115133451E-4	0.0	0.0	8.59117160525608	0.0
21	3.4631115133451E-4	0.0	0.0	8.815119483119062	0.0
22	3.4631115133451E-4	0.0	0.0	9.055459422145212	0.0
23	3.4631115133451E-4	0.0	0.0	9.28783420469067	0.0
24	3.4631115133451E-4	0.0	0.0	9.48476981274956	0.0
25	3.4631115133451E-4	0.0	0.0	9.66877647115863	0.0
26	3.4631115133451E-4	0.0	0.0	9.862248967704176	0.0
27	3.4631115133451E-4	0.0	0.0	10.078347126136912	0.0
28	3.4631115133451E-4	0.0	0.0	10.277014289952474	0.0
29	3.4631115133451E-4	0.0	0.0	10.484916417803625	0.0
30	3.4631115133451E-4	0.0	0.0	10.752730374835647	0.0
31	3.4631115133451E-4	0.0	0.0	10.985105157381103	0.0
32	3.4631115133451E-4	0.0	0.0	11.227176652163925	0.0
33	3.4631115133451E-4	0.0	0.0	11.464976976080289	0.0
34	3.4631115133451E-4	0.0	0.0	11.730828503254747	0.0
35	3.4631115133451E-4	0.0	0.0	11.995410222874312	0.0
36	3.4631115133451E-4	0.0	0.0	12.23875152521203	0.0
37	3.4631115133451E-4	0.0	0.0	12.494444591947342	0.0
38	3.4631115133451E-4	0.0	0.0	12.791810433893241	0.0
39	3.4631115133451E-4	0.0	0.0	13.256906310135488	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACGG	25	3.890007E-5	45.0	2
CGCATTC	30	2.1648812E-6	44.999996	27
CCATACG	120	0.0	43.124996	2
CGTTTTT	6495	0.0	42.74827	1
CGTTTTA	415	0.0	40.12048	1
CGAACCC	145	0.0	38.793106	19
CCGCATG	160	0.0	37.96875	24
ACGGGAC	90	0.0	37.5	5
GCGTTAG	55	2.748493E-9	36.81818	1
TAGACGG	80	0.0	36.5625	2
CCGAACC	155	0.0	36.29032	18
GTTTTTT	7955	0.0	36.090508	2
CATACGA	75	1.8189894E-12	36.000004	18
CGTTAGG	100	0.0	36.0	2
GTCGTAC	25	0.0021068226	36.0	37
ATACCGG	50	4.8765287E-8	36.0	2
CTCGTAA	115	0.0	35.21739	33
TCCGAAC	160	0.0	35.15625	17
ACGTCAT	90	0.0	35.0	35
ATAACGG	45	8.696643E-7	35.0	2
>>END_MODULE