##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936629.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1061044
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1550868766988	31.0	31.0	33.0	28.0	34.0
2	31.207123361519407	31.0	31.0	34.0	28.0	34.0
3	30.919523601283263	31.0	31.0	34.0	27.0	34.0
4	34.89491764714753	35.0	35.0	37.0	32.0	37.0
5	35.15346677423368	35.0	35.0	37.0	33.0	37.0
6	35.13968977723827	36.0	35.0	37.0	32.0	37.0
7	30.955908520287565	35.0	35.0	37.0	0.0	37.0
8	33.02880653394204	36.0	35.0	37.0	17.0	37.0
9	36.363300673676115	39.0	35.0	39.0	32.0	39.0
10	36.11919109857838	37.0	35.0	39.0	32.0	39.0
11	36.39609950199992	38.0	35.0	39.0	32.0	39.0
12	36.53515028594479	38.0	35.0	39.0	32.0	39.0
13	36.53673928696642	39.0	35.0	39.0	32.0	39.0
14	37.61076731973415	39.0	36.0	41.0	33.0	41.0
15	37.76946950362096	40.0	37.0	41.0	33.0	41.0
16	37.70369937533222	39.0	36.0	41.0	33.0	41.0
17	37.57284429297937	39.0	36.0	41.0	32.0	41.0
18	37.46015905089704	39.0	36.0	40.0	32.0	41.0
19	37.257787612954786	39.0	36.0	40.0	32.0	41.0
20	37.187729255337196	39.0	35.0	40.0	32.0	41.0
21	37.223728704935894	39.0	35.0	40.0	32.0	41.0
22	37.308598889395725	39.0	35.0	40.0	32.0	41.0
23	37.359557190842224	39.0	35.0	40.0	32.0	41.0
24	37.3017839034008	39.0	35.0	41.0	32.0	41.0
25	36.993345233562415	39.0	35.0	40.0	32.0	41.0
26	36.97390683138494	39.0	35.0	40.0	32.0	41.0
27	37.00944729907525	39.0	35.0	40.0	32.0	41.0
28	36.98210630284889	39.0	35.0	40.0	32.0	41.0
29	36.94196187905497	39.0	35.0	40.0	31.0	41.0
30	36.71524555060864	39.0	35.0	40.0	31.0	41.0
31	36.64992592201643	39.0	35.0	40.0	31.0	41.0
32	36.45611397830816	39.0	35.0	40.0	30.0	41.0
33	36.26106457413642	39.0	35.0	40.0	30.0	41.0
34	36.1059607330139	39.0	35.0	40.0	29.0	41.0
35	36.02060800494607	39.0	35.0	40.0	29.0	41.0
36	35.940219255751884	39.0	35.0	40.0	29.0	41.0
37	35.87128431997165	39.0	35.0	40.0	28.0	41.0
38	35.742718492352815	39.0	35.0	40.0	27.0	41.0
39	35.660350560391464	38.0	35.0	40.0	27.0	41.0
40	35.56958523868944	38.0	35.0	40.0	26.0	41.0
41	35.41267185903695	38.0	35.0	40.0	26.0	41.0
42	35.438037442368085	38.0	35.0	40.0	26.0	41.0
43	35.41062293363895	38.0	35.0	40.0	26.0	41.0
44	35.23541342300602	38.0	34.0	40.0	25.0	41.0
45	35.16238063642978	38.0	34.0	40.0	25.0	41.0
46	35.06611884144296	38.0	34.0	40.0	25.0	41.0
47	34.998361990643176	38.0	34.0	40.0	24.0	41.0
48	34.92235666004426	38.0	34.0	40.0	24.0	41.0
49	34.81702738057988	38.0	34.0	40.0	24.0	41.0
50	34.672595104444305	38.0	34.0	40.0	24.0	41.0
51	33.00437210897946	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	21.0
9	68.0
10	99.0
11	71.0
12	47.0
13	42.0
14	52.0
15	96.0
16	145.0
17	282.0
18	602.0
19	1034.0
20	1702.0
21	2671.0
22	4056.0
23	5922.0
24	8688.0
25	12099.0
26	15454.0
27	17005.0
28	17838.0
29	19534.0
30	23453.0
31	29376.0
32	37546.0
33	51959.0
34	75105.0
35	86611.0
36	106145.0
37	158175.0
38	211432.0
39	173703.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.08005323059176	21.350292730555942	24.247250820889615	14.322403217962687
2	35.71821715216334	24.521226263943813	23.543415730167645	16.217140853725198
3	31.11633447811778	24.14923415051591	29.30491101217292	15.429520359193399
4	27.747576914812207	24.38664183577684	29.603673363215847	18.262107886195107
5	25.38414995042618	28.008452052883765	27.941913813187767	18.665484183502286
6	25.70025371238139	33.70039319764307	27.408005700046367	13.191347389929165
7	67.19495138750136	17.565435552154295	11.655124575418172	3.5844884849261667
8	79.19087238606505	5.79778029940323	11.025084727871794	3.9862625866599313
9	72.79679259295561	7.271517486550982	13.107938973313077	6.823750947180324
10	37.31390969648761	28.654985090156487	20.173338711683964	13.85776650167194
11	27.28774678524171	26.112866195935325	28.658849208892374	17.940537809930596
12	24.97832323635966	23.24041227319508	32.66839075476606	19.112873735679198
13	23.32777905534549	23.852922216232315	34.11573883835166	18.703559890070533
14	19.73452561816475	27.40319911332612	32.6352158817165	20.227059386792632
15	19.185255276878245	26.50936247695666	35.60408427925703	18.701297966908065
16	22.90451668356826	26.371196670449105	31.698779692453847	19.02550695352879
17	23.18904776804732	25.66924651569586	30.534643238169203	20.60706247808762
18	23.79081357606282	25.042128318901007	31.47060819343967	19.696449911596503
19	23.003852809120072	27.477654084090762	29.652398958007396	19.866094148781766
20	23.95706492850438	27.074277786783586	30.622952488303973	18.345704796408064
21	23.58139718993746	27.21291482728332	31.51707186506874	17.68861611771048
22	23.16539182163982	24.31256385220594	31.133110408239435	21.38893391791481
23	20.966142780129758	26.323979024432543	32.565379004075226	20.14449919136247
24	22.021801169414275	25.27416393665107	31.76974753167635	20.934287362258306
25	21.801169414274998	27.973392243865476	29.83429527898937	20.391143062870153
26	21.165192018427135	26.510210698142583	30.200726831309538	22.12387045212074
27	21.325600069365645	26.246508156117937	30.881188716019313	21.5467030584971
28	19.982394698052108	26.73008847889437	31.947496993527132	21.34001982952639
29	20.568704031124064	26.55714560376384	31.88048751983895	20.993662845273146
30	21.73783556572583	25.690357798545584	31.522066945385863	21.04973969034272
31	23.377824105315142	26.140480507877147	29.218015464014684	21.26367992279302
32	22.48417596254255	27.91260305887409	29.462680152755212	20.140540825828147
33	21.705226173466887	26.07818337411078	28.79871145777178	23.41787899465055
34	20.923448980438135	27.51601253105432	30.609852183321333	20.950686305186213
35	19.9639223255586	27.37096670826092	30.621538786327427	22.04357217985305
36	21.41504028108165	27.8237283279487	28.921609282932657	21.83962210803699
37	20.94465451008629	28.34679805926993	29.182013187012036	21.526534243631744
38	21.157935014947544	28.24727344012124	28.192987284221953	22.401804260709262
39	21.510700781494453	27.207071525780268	28.51917545360984	22.763052239115435
40	22.13395485955342	26.081105024862307	30.558487678173574	21.2264524374107
41	19.83650065407278	27.36446367916882	29.710926219836313	23.08810944692209
42	20.739384983092123	26.415021431721964	30.80663949845624	22.038954086729674
43	21.760454797350533	25.68083887190352	29.050633149991896	23.50807318075405
44	21.093658698414014	26.550265587477995	28.966282265391445	23.38979344871655
45	21.229185594565354	25.820324133589185	28.890884826642438	24.05960544520303
46	21.91388858520476	26.807653594007412	28.43765197296248	22.840805847825347
47	19.88560323605807	26.52915430462827	31.19380534643238	22.391437112881277
48	20.543540135941583	26.343016877716664	30.150493287743014	22.962949698598738
49	20.773408077327613	25.469443303010998	31.13716302057219	22.619985599089198
50	19.687119478551313	26.073659527785843	31.37362823784876	22.865592755814085
51	19.818688009168326	25.090476926498805	29.65126799642616	25.439567067906705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	383.0
1	866.0
2	1349.0
3	8883.0
4	16417.0
5	11275.0
6	6133.0
7	5728.5
8	5324.0
9	5160.0
10	4996.0
11	4884.0
12	4772.0
13	4608.0
14	4444.0
15	4287.5
16	4131.0
17	4115.0
18	4099.0
19	4160.0
20	4221.0
21	4344.0
22	4467.0
23	4990.0
24	5513.0
25	6686.5
26	9826.0
27	11792.0
28	13788.5
29	15785.0
30	18823.0
31	21861.0
32	25018.5
33	28176.0
34	31867.0
35	35558.0
36	38264.0
37	40970.0
38	44352.0
39	47734.0
40	51450.5
41	55167.0
42	59181.0
43	63195.0
44	67197.5
45	71200.0
46	87116.0
47	103032.0
48	95408.0
49	87784.0
50	85267.5
51	82751.0
52	74029.0
53	65307.0
54	60466.0
55	55625.0
56	51143.0
57	46661.0
58	44286.5
59	41912.0
60	38358.5
61	34805.0
62	30868.5
63	26932.0
64	22852.5
65	18773.0
66	15458.0
67	12143.0
68	10080.0
69	8017.0
70	6467.0
71	4917.0
72	3929.5
73	2942.0
74	2473.5
75	1534.0
76	1063.0
77	744.5
78	426.0
79	319.5
80	213.0
81	154.5
82	96.0
83	68.5
84	41.0
85	30.0
86	19.0
87	15.0
88	11.0
89	9.5
90	8.0
91	8.0
92	8.0
93	6.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1061044.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.660836724867835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.17669875233479	24.96849745492049
2	8.803718546697485	5.926810651441496
3	3.770579693925614	3.8076260230599663
4	2.3484091120783415	3.161976627394435
5	1.5825314430672897	2.6634666258528754
6	1.1518327436552778	2.3262992351122107
7	0.8395700527115537	1.9782441324388604
8	0.6645192538438638	1.789461928333544
9	0.5361007803294445	1.6241040751269302
>10	5.925367457804002	43.28407662905918
>50	0.17137356418710478	3.484955278352276
>100	0.024599767393049657	1.5262148037400842
>500	0.002764018807169749	0.7164947696348302
>1k	0.0013820094035848745	1.2805913529741335
>5k	5.528037614339498E-4	1.4611804125587595
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9205	0.8675417796057467	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	6500	0.6126041898356712	RNA PCR Primer, Index 41 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG	4460	0.4203407210257068	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC	3832	0.3611537316077373	No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2060	0.19414840477868966	Illumina PCR Primer Index 11 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	2019	0.19028428604280312	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT	1393	0.13128579022170617	RNA PCR Primer, Index 41 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8849359687251425E-4	0.0	0.0	0.10348298468301032	0.0
2	1.8849359687251425E-4	0.0	0.0	0.4751923577156084	0.0
3	1.8849359687251425E-4	0.0	0.0	0.6618010186193976	0.0
4	1.8849359687251425E-4	0.0	0.0	1.0815762588544866	0.0
5	1.8849359687251425E-4	0.0	0.0	2.2775681310105895	0.0
6	1.8849359687251425E-4	0.0	0.0	2.9710360739045694	0.0
7	1.8849359687251425E-4	0.0	0.0	3.4563128390528575	0.0
8	1.8849359687251425E-4	0.0	0.0	4.135266774987653	0.0
9	1.8849359687251425E-4	0.0	0.0	4.4273376033416145	0.0
10	1.8849359687251425E-4	0.0	0.0	5.418813922891039	0.0
11	1.8849359687251425E-4	0.0	0.0	6.085233034633814	0.0
12	1.8849359687251425E-4	0.0	0.0	7.048341067853925	0.0
13	1.8849359687251425E-4	0.0	0.0	7.362277153445097	0.0
14	1.8849359687251425E-4	0.0	0.0	7.530978922645998	0.0
15	1.8849359687251425E-4	0.0	0.0	7.761412344822646	0.0
16	1.8849359687251425E-4	0.0	0.0	8.129917326708412	0.0
17	1.8849359687251425E-4	0.0	0.0	8.561944650740214	0.0
18	1.8849359687251425E-4	0.0	0.0	9.034403851301171	0.0
19	1.8849359687251425E-4	0.0	0.0	9.36756628377334	0.0
20	1.8849359687251425E-4	0.0	0.0	9.673585638295867	0.0
21	1.8849359687251425E-4	0.0	0.0	10.034268135911423	0.0
22	1.8849359687251425E-4	0.0	0.0	10.433497574087408	0.0
23	1.8849359687251425E-4	0.0	0.0	10.832444271868084	0.0
24	1.8849359687251425E-4	0.0	0.0	11.16268505358873	0.0
25	2.8274039530877136E-4	0.0	0.0	11.453342179966146	0.0
26	2.8274039530877136E-4	0.0	0.0	11.735611341282736	0.0
27	2.8274039530877136E-4	0.0	0.0	12.041913436200572	0.0
28	2.8274039530877136E-4	0.0	0.0	12.358111444954215	0.0
29	2.8274039530877136E-4	0.0	0.0	12.686750031101443	0.0
30	2.8274039530877136E-4	0.0	0.0	13.067884083977667	0.0
31	2.8274039530877136E-4	0.0	0.0	13.41329860024655	0.0
32	2.8274039530877136E-4	0.0	0.0	13.752398581020202	0.0
33	2.8274039530877136E-4	0.0	0.0	14.100357760846864	0.0
34	2.8274039530877136E-4	0.0	0.0	14.43483964849714	0.0
35	2.8274039530877136E-4	0.0	0.0	14.795616392911132	0.0
36	2.8274039530877136E-4	0.0	0.0	15.132360203723879	0.0
37	3.769871937450285E-4	0.0	0.0	15.471271690900661	0.0
38	3.769871937450285E-4	0.0	0.0	15.841473115158278	0.0
39	3.769871937450285E-4	0.0	0.0	16.316382732478576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	25	3.8906015E-5	45.000004	1
CGTTTTT	5160	0.0	42.1657	1
CGAATAT	120	0.0	41.249996	14
ACAACGA	260	0.0	40.67308	13
CGGTCTA	50	1.0822987E-9	40.500004	31
TCGATAG	35	6.2478357E-6	38.571426	1
TACGGCT	485	0.0	38.505157	7
AGCGACT	270	0.0	38.333336	19
GACCGAT	415	0.0	37.95181	9
CGTTTTA	305	0.0	37.622948	1
CGTCTAC	30	1.1398629E-4	37.499996	36
TATTGCG	30	1.1398629E-4	37.499996	1
ACGGGAC	265	0.0	37.35849	5
ATTTGCG	55	2.748493E-9	36.81818	1
AGTACGG	110	0.0	36.81818	2
CACAACG	290	0.0	36.465515	12
CCGGATA	25	0.0021070354	36.000004	19
TTCGTAA	50	4.8783477E-8	36.000004	44
CAATTCG	25	0.0021070354	36.000004	39
CGAATTT	25	0.0021070354	36.000004	16
>>END_MODULE