##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936628.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	777772
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.124699783484107	31.0	31.0	33.0	28.0	34.0
2	31.16954068801654	31.0	31.0	34.0	28.0	34.0
3	30.933429076901714	31.0	31.0	34.0	27.0	34.0
4	34.92155927444033	35.0	35.0	37.0	32.0	37.0
5	35.168827111287115	35.0	35.0	37.0	33.0	37.0
6	35.13937760680508	36.0	35.0	37.0	32.0	37.0
7	31.005717613902274	35.0	35.0	37.0	0.0	37.0
8	33.08377647948242	36.0	35.0	37.0	17.0	37.0
9	36.42352771763447	39.0	35.0	39.0	32.0	39.0
10	35.98961520857012	37.0	35.0	39.0	32.0	39.0
11	36.32382369126171	37.0	35.0	39.0	32.0	39.0
12	36.472052220959355	38.0	35.0	39.0	32.0	39.0
13	36.48732404869293	39.0	35.0	39.0	32.0	39.0
14	37.55459697700611	39.0	36.0	40.0	33.0	41.0
15	37.73915363371271	39.0	37.0	41.0	33.0	41.0
16	37.65712188147683	39.0	36.0	41.0	33.0	41.0
17	37.532924816012915	39.0	36.0	41.0	32.0	41.0
18	37.46688103968772	39.0	36.0	40.0	32.0	41.0
19	37.27351974614668	39.0	36.0	40.0	32.0	41.0
20	37.29478947557896	39.0	36.0	40.0	32.0	41.0
21	37.30267353414625	39.0	36.0	40.0	32.0	41.0
22	37.38864860138961	39.0	36.0	40.0	32.0	41.0
23	37.42705060094732	39.0	36.0	40.0	32.0	41.0
24	37.385844437701536	39.0	36.0	41.0	32.0	41.0
25	37.05738699773198	39.0	35.0	40.0	31.0	41.0
26	37.08333419048256	39.0	35.0	40.0	32.0	41.0
27	37.13020396722947	39.0	35.0	40.0	32.0	41.0
28	37.148915106226504	39.0	35.0	40.0	32.0	41.0
29	37.13912046203772	39.0	35.0	40.0	32.0	41.0
30	36.93826982714729	39.0	35.0	40.0	31.0	41.0
31	36.935339662523205	39.0	35.0	40.0	31.0	41.0
32	36.814410907052455	39.0	35.0	40.0	31.0	41.0
33	36.70387337163076	39.0	35.0	40.0	31.0	41.0
34	36.64975982678728	39.0	35.0	40.0	30.0	41.0
35	36.59236382898845	39.0	35.0	40.0	30.0	41.0
36	36.559023724176235	39.0	35.0	40.0	30.0	41.0
37	36.52185473377802	39.0	35.0	40.0	30.0	41.0
38	36.41355950072772	39.0	35.0	40.0	30.0	41.0
39	36.37734708886409	39.0	35.0	40.0	30.0	41.0
40	36.30581455747957	39.0	35.0	40.0	30.0	41.0
41	36.169324686411954	39.0	35.0	40.0	30.0	41.0
42	36.20791825882135	39.0	35.0	40.0	30.0	41.0
43	36.16535179975622	39.0	35.0	40.0	30.0	41.0
44	35.99973642661345	39.0	35.0	40.0	29.0	41.0
45	35.89202876935657	38.0	35.0	40.0	29.0	41.0
46	35.78939972125507	38.0	35.0	40.0	28.0	41.0
47	35.77276116908297	38.0	35.0	40.0	28.0	41.0
48	35.71454359375241	38.0	35.0	40.0	28.0	41.0
49	35.61732744300386	38.0	35.0	40.0	28.0	41.0
50	35.45990727359689	38.0	34.0	40.0	27.0	41.0
51	33.91736781473234	37.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	33.0
9	50.0
10	60.0
11	56.0
12	32.0
13	27.0
14	35.0
15	43.0
16	99.0
17	171.0
18	349.0
19	611.0
20	1000.0
21	1515.0
22	2227.0
23	3277.0
24	4719.0
25	6465.0
26	8046.0
27	9599.0
28	10676.0
29	12783.0
30	16067.0
31	20614.0
32	26741.0
33	37266.0
34	55966.0
35	63657.0
36	78037.0
37	119695.0
38	164257.0
39	133593.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11041282020952	21.87710537278277	25.298416502522592	10.71406530448512
2	33.85979438704402	24.67034040824303	24.297737640336756	17.17212756437619
3	32.14695309165154	24.390824046121484	27.28858842951405	16.17363443271293
4	28.056808422005417	26.198551760670224	26.479353846628573	19.265285970695782
5	26.039379149673685	30.11216654752292	25.377359946102455	18.471094356700934
6	25.805120266607695	34.827173001856586	25.78956300818235	13.578143723353373
7	70.51668098105873	17.597573581975183	8.606635363576986	3.2791100733891168
8	82.88804431118632	5.348482588727802	8.109445955884244	3.6540271442016428
9	76.83279932936644	7.396383515991833	9.85571607103367	5.915101083608049
10	36.82313068611367	30.642784774972615	18.446537031417947	14.08754750749577
11	27.674177008172062	27.66324835555921	26.71926991457651	17.94330472169222
12	24.9642568773368	24.745812397463524	30.81237174904728	19.477558976152395
13	23.643175635019002	26.850413745930684	31.301846813719187	18.204563805331127
14	20.937241248077843	29.73454945665311	29.557119567173928	19.771089728095124
15	19.800918521109015	28.652741420364837	33.18221792504744	18.364122133478705
16	23.364044990048498	27.524904470718926	30.417911675915306	18.693138863317273
17	22.922398852105758	27.246545260050503	28.700698919477684	21.13035696836605
18	24.8279701506354	26.153937143533067	29.469690346271143	19.548402359560384
19	23.103557340711674	29.03652998565132	28.047936927531463	19.811975746105542
20	24.69091198963192	27.21581646035085	29.840750245573254	18.252521304443974
21	23.087357220367924	28.74274208894123	29.947979613562843	18.221921077128002
22	22.96817062069604	24.781812664894083	30.125666647809386	22.124350066600496
23	20.709668128963244	29.009015495543682	29.978194123727775	20.3031222517653
24	21.451016493265378	26.08875094500702	31.451247923556004	21.008984638171597
25	20.377437089532666	30.246524688469112	28.72127050086658	20.65476772113164
26	21.071085099489313	28.810114017989847	29.02508704350375	21.093713839017088
27	21.148871391616055	29.025729905422153	28.948715047597496	20.876683655364296
28	19.759389641180192	28.2971102071044	30.709128124951786	21.234372026763626
29	20.203735799180222	27.4324609268526	30.201010064646194	22.162793209320984
30	21.204286088982375	27.722648796819634	29.79446418744825	21.278600926749743
31	21.725775677190747	28.272295737054048	28.16455207952973	21.837376506225475
32	21.553488683058788	28.84457141681624	28.56274075178844	21.03919914833653
33	21.266129405532727	28.613012553807543	28.47068292507316	21.650175115586574
34	19.70873212201005	28.954757949630483	28.996286829559303	22.340223098800163
35	19.61101711041282	29.212417006526337	29.784306969137486	21.39225891392336
36	20.91204106087645	29.443204435232946	28.62676979886136	21.01798470502924
37	20.595624424638583	28.53972629510962	29.524333609335386	21.340315670916414
38	19.56280246653261	30.266453407939604	28.074037121418616	22.096707004109174
39	20.425394588645513	27.986350755748475	29.79446418744825	21.793790468157763
40	21.54526005050323	28.713427585462064	28.961700858349232	20.779611505685473
41	19.29858621806905	28.501926014307532	28.86771444587874	23.331773321744677
42	21.199400338402512	27.756334761343943	29.159573756833623	21.884691143419925
43	21.75701876642512	26.735341462536578	29.250088714944738	22.25755105609356
44	21.11904259860216	27.41484651028836	28.215209598699875	23.2509012924096
45	20.443266149977113	27.62184804801407	28.668427251173867	23.266458550834948
46	21.503088308656007	29.205345525423905	27.15487315048626	22.136693015433828
47	19.137742166084664	29.012872667054097	29.691349135736438	22.1580360311248
48	20.413951646497946	28.089980096995003	29.328903586140925	22.167164670366123
49	20.994455958815696	26.918814254048744	30.254881893408353	21.83184789372721
50	19.134270711725286	27.64871967620331	29.982822729540278	23.234186882531127
51	19.2691431422062	27.230345139706753	28.694141727909976	24.80636999017707
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	325.0
1	693.0
2	1061.0
3	2960.0
4	4859.0
5	3597.5
6	2336.0
7	2240.5
8	2145.0
9	2122.0
10	2099.0
11	2019.5
12	1940.0
13	1950.0
14	1960.0
15	1968.0
16	1976.0
17	2151.5
18	2327.0
19	2622.0
20	2917.0
21	2949.5
22	2982.0
23	3466.5
24	3951.0
25	4859.5
26	6919.5
27	8071.0
28	10024.5
29	11978.0
30	13904.5
31	15831.0
32	18380.5
33	20930.0
34	25015.0
35	29100.0
36	31629.0
37	34158.0
38	36711.5
39	39265.0
40	42993.0
41	46721.0
42	51953.0
43	57185.0
44	66306.0
45	75427.0
46	73661.5
47	71896.0
48	70816.5
49	69737.0
50	64887.5
51	60038.0
52	54139.0
53	48240.0
54	43987.5
55	39735.0
56	35958.0
57	32181.0
58	28276.5
59	24372.0
60	21916.5
61	19461.0
62	16820.5
63	14180.0
64	11602.5
65	9025.0
66	7228.5
67	5432.0
68	4558.0
69	3684.0
70	2759.5
71	1835.0
72	1539.5
73	1244.0
74	999.0
75	551.5
76	349.0
77	237.0
78	125.0
79	111.0
80	97.0
81	57.5
82	18.0
83	19.5
84	21.0
85	15.5
86	10.0
87	10.5
88	11.0
89	8.0
90	5.0
91	4.0
92	3.0
93	3.0
94	3.0
95	2.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	777772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.75299631378682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.50532219378614	24.40272543170576
2	9.270309826783159	6.07260847168581
3	3.7057531863639532	3.64123561358347
4	2.1057512887735124	2.7587865679580275
5	1.4769208998319996	2.4186792393976093
6	1.081523837429358	2.12538877563578
7	0.832785851473705	1.9093362346444451
8	0.6290353661872558	1.6482234423978182
9	0.495970201540145	1.4620059164513226
>10	5.451713170271435	42.07113753966738
>50	0.40748055747673656	8.053830591203209
>100	0.03470408528493133	1.9652771706512175
>500	7.798670850546366E-4	0.13908154658429786
>1k	0.001949667712636591	1.3316834584338604
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG	2377	0.30561655600870175	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	2330	0.2995736539757152	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC	2224	0.28594498130557544	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2202	0.283116388864603	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	1294	0.16637266448265045	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2857238368056448E-4	0.0	0.0	0.0821577531718807	0.0
2	1.2857238368056448E-4	0.0	0.0	0.44010326933857224	0.0
3	1.2857238368056448E-4	0.0	0.0	0.5838471942934433	0.0
4	1.2857238368056448E-4	0.0	0.0	0.8944780732656871	0.0
5	1.2857238368056448E-4	0.0	0.0	1.7805989415921375	0.0
6	1.2857238368056448E-4	0.0	0.0	2.2423023713890444	0.0
7	1.2857238368056448E-4	0.0	0.0	2.6384338855088636	0.0
8	1.2857238368056448E-4	0.0	0.0	3.3478962986582186	0.0
9	1.2857238368056448E-4	0.0	0.0	3.5584978631269832	0.0
10	1.2857238368056448E-4	0.0	0.0	4.442690145698225	0.0
11	1.2857238368056448E-4	0.0	0.0	5.300525089614951	0.0
12	1.2857238368056448E-4	0.0	0.0	6.2694465730316855	0.0
13	1.2857238368056448E-4	0.0	0.0	6.587791795024763	0.0
14	1.2857238368056448E-4	0.0	0.0	6.741178648755676	0.0
15	1.2857238368056448E-4	0.0	0.0	6.998966278035208	0.0
16	1.2857238368056448E-4	0.0	0.0	7.4957699685769095	0.0
17	1.2857238368056448E-4	0.0	0.0	8.13130326110994	0.0
18	1.2857238368056448E-4	0.0	0.0	8.816208348976307	0.0
19	1.2857238368056448E-4	0.0	0.0	9.248468702910364	0.0
20	1.2857238368056448E-4	0.0	0.0	9.65051454667949	0.0
21	1.2857238368056448E-4	0.0	0.0	10.187432820929526	0.0
22	1.2857238368056448E-4	0.0	0.0	10.726279680934773	0.0
23	1.2857238368056448E-4	0.0	0.0	11.295598195872312	0.0
24	1.2857238368056448E-4	0.0	0.0	11.701244066384493	0.0
25	1.2857238368056448E-4	0.0	0.0	12.102261331084174	0.0
26	1.2857238368056448E-4	0.0	0.0	12.479364132419269	0.0
27	1.2857238368056448E-4	0.0	0.0	12.880638541886311	0.0
28	1.2857238368056448E-4	0.0	0.0	13.275870049320366	0.0
29	1.2857238368056448E-4	0.0	0.0	13.692316000061714	0.0
30	1.2857238368056448E-4	0.0	0.0	14.179991051362096	0.0
31	1.2857238368056448E-4	0.0	0.0	14.618037162561778	0.0
32	1.2857238368056448E-4	0.0	0.0	15.043997469695489	0.0
33	2.5714476736112896E-4	0.0	0.0	15.475872106478505	0.0
34	2.5714476736112896E-4	0.0	0.0	15.881260832223326	0.0
35	2.5714476736112896E-4	0.0	0.0	16.322006963480302	0.0
36	2.5714476736112896E-4	0.0	0.0	16.731638577886578	0.0
37	3.8571715104169347E-4	0.0	0.0	17.1694275443189	0.0
38	3.8571715104169347E-4	0.0	0.0	17.62765951975643	0.0
39	3.8571715104169347E-4	0.0	0.0	18.093220121063755	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGCTA	20	7.0318783E-4	45.0	44
TAACGCC	20	7.0318783E-4	45.0	12
CTTATCG	20	7.0318783E-4	45.0	32
TGTTACG	20	7.0318783E-4	45.0	1
GAACCGG	25	3.889638E-5	45.0	2
ATACGTC	25	3.889638E-5	45.0	42
TGTCGTA	20	7.0318783E-4	45.0	44
CGATAAT	20	7.0318783E-4	45.0	36
TCGGTAA	20	7.0318783E-4	45.0	41
TACGTCC	20	7.0318783E-4	45.0	12
TATCGCG	30	2.1645938E-6	44.999996	1
CGTTTTA	120	0.0	43.124996	1
CGAACGG	65	0.0	41.538464	2
GCGAACG	50	1.0804797E-9	40.5	1
CGTTTTT	1595	0.0	40.485893	1
CGCCGGT	145	0.0	40.344826	28
CTACGGG	190	0.0	40.26316	3
ACAACGT	45	1.9268555E-8	40.0	22
GGCTACG	45	1.9268555E-8	40.0	1
TTGCGCG	85	0.0	39.705883	1
>>END_MODULE