##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936625.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	835004
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1856649788504	31.0	31.0	33.0	28.0	34.0
2	31.22255222729472	31.0	31.0	34.0	28.0	34.0
3	31.045774631019732	31.0	31.0	34.0	28.0	34.0
4	35.00548620126371	35.0	35.0	37.0	32.0	37.0
5	35.207422958452895	35.0	35.0	37.0	33.0	37.0
6	35.18039913581252	36.0	35.0	37.0	32.0	37.0
7	31.046487202456515	35.0	35.0	37.0	0.0	37.0
8	33.05431710506776	35.0	35.0	37.0	17.0	37.0
9	36.30338657060325	39.0	35.0	39.0	32.0	39.0
10	36.10435279351955	37.0	35.0	39.0	32.0	39.0
11	36.39730588116943	37.0	35.0	39.0	32.0	39.0
12	36.48078811598507	38.0	35.0	39.0	32.0	39.0
13	36.423314139812504	39.0	35.0	39.0	32.0	39.0
14	37.51519393919071	39.0	36.0	40.0	32.0	41.0
15	37.66571776901667	39.0	36.0	41.0	33.0	41.0
16	37.633022117259316	39.0	36.0	41.0	33.0	41.0
17	37.517956800206946	39.0	36.0	41.0	32.0	41.0
18	37.45157627987411	39.0	36.0	40.0	32.0	41.0
19	37.261059827258315	39.0	36.0	40.0	32.0	41.0
20	37.239032387868804	39.0	35.0	40.0	32.0	41.0
21	37.28793754281416	39.0	35.0	40.0	32.0	41.0
22	37.3764640648428	39.0	36.0	40.0	32.0	41.0
23	37.453397828034355	39.0	36.0	41.0	33.0	41.0
24	37.39658253134117	39.0	35.0	41.0	32.0	41.0
25	37.10458991813213	39.0	35.0	40.0	32.0	41.0
26	37.05475662392036	39.0	35.0	40.0	32.0	41.0
27	37.082963674425514	39.0	35.0	40.0	32.0	41.0
28	37.06159850731254	39.0	35.0	40.0	32.0	41.0
29	37.048885993360514	39.0	35.0	40.0	31.0	41.0
30	36.876671249479045	39.0	35.0	40.0	31.0	41.0
31	36.8886220904331	39.0	35.0	40.0	31.0	41.0
32	36.76915799205752	39.0	35.0	40.0	31.0	41.0
33	36.65650104670157	39.0	35.0	40.0	31.0	41.0
34	36.57915051903943	39.0	35.0	40.0	30.0	41.0
35	36.56951104425847	39.0	35.0	40.0	30.0	41.0
36	36.5014191548783	39.0	35.0	40.0	30.0	41.0
37	36.42203510402345	39.0	35.0	40.0	30.0	41.0
38	36.33409061513478	39.0	35.0	40.0	30.0	41.0
39	36.259663426762025	39.0	35.0	40.0	30.0	41.0
40	36.16148186116474	39.0	35.0	40.0	30.0	41.0
41	35.97481688710473	38.0	35.0	40.0	29.0	41.0
42	35.97812106289311	38.0	35.0	40.0	29.0	41.0
43	35.92934524864552	38.0	35.0	40.0	29.0	41.0
44	35.7700274489703	38.0	35.0	40.0	29.0	41.0
45	35.72874261680184	38.0	35.0	40.0	28.0	41.0
46	35.60970366609022	38.0	35.0	40.0	28.0	41.0
47	35.54126447298456	38.0	34.0	40.0	28.0	41.0
48	35.45130442488898	38.0	34.0	40.0	27.0	41.0
49	35.36316233215649	38.0	34.0	40.0	27.0	41.0
50	35.2205654104651	38.0	34.0	40.0	27.0	41.0
51	33.60210130729913	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	35.0
9	71.0
10	73.0
11	68.0
12	44.0
13	31.0
14	36.0
15	44.0
16	102.0
17	189.0
18	323.0
19	683.0
20	1233.0
21	2020.0
22	2780.0
23	3760.0
24	5191.0
25	6926.0
26	8437.0
27	10104.0
28	11637.0
29	13857.0
30	17344.0
31	22539.0
32	29707.0
33	41366.0
34	62822.0
35	70000.0
36	84097.0
37	126906.0
38	171900.0
39	140671.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.24757246671872	20.628284415403996	23.904675905744163	11.219467212133114
2	32.37541377047295	26.297359054567405	24.75784547139894	16.5693817035607
3	32.674334494206015	25.06215539087238	26.66645908283074	15.597051032090864
4	28.683694928407526	25.22790309986539	27.532802238073113	18.55559973365397
5	27.478790520763972	29.569319428409923	24.47329593630689	18.47859411451921
6	25.989695857744394	35.96258221517502	24.37221857619844	13.67550335088215
7	68.92829255907756	17.97931506914937	9.555044047693183	3.5373483240798844
8	80.43266858601875	7.621759895760978	8.238643168176441	3.7069283500438326
9	73.63377899986108	8.164511786769884	10.329890635254442	7.871818578114596
10	40.16304113513229	27.376156281886075	17.804585367255726	14.656217215725913
11	30.402369329967282	26.407777687292516	25.342273809466782	17.84757917327342
12	28.01375801792566	24.405751349694132	28.220224094734874	19.36026653764533
13	25.696763129278423	24.438805083568464	30.65446393071171	19.209967856441406
14	21.327322982883913	28.21172114145561	29.121058102715676	21.3398977729448
15	19.551523106476136	26.810650008862236	34.10917792010577	19.52864896455586
16	23.14611666530939	26.430891349023476	29.928958424151265	20.494033561515874
17	23.33761275395088	25.417602789926757	28.990879085609173	22.253905370513195
18	24.061681141647227	26.233167745304215	29.092435485338992	20.61271562770957
19	24.54287644131046	27.093403145374158	27.4172339294183	20.946486483897083
20	26.168257876608976	26.760710128334715	28.2446551154246	18.826376879631717
21	25.059999712576225	27.97519532840561	28.337469042064466	18.62733591695369
22	23.756532902836394	25.09053848843838	29.08680676978793	22.0661218389373
23	22.74779522014266	26.80921288999813	29.60357076133767	20.83942112852154
24	22.75749577247534	25.696763129278423	29.5486009647858	21.997140133460437
25	22.343366019803497	28.19950563111075	27.76717237282696	21.689955976258794
26	21.28840101364784	27.63651431609909	28.902256755656257	22.172827914596816
27	22.212468443264942	27.749567666741715	28.621300017724465	21.416663872268877
28	19.923976412089043	27.344180387159824	30.605601889332267	22.126241311418866
29	22.26037240540165	26.49807665592021	29.726324664312987	21.515226274365155
30	21.623608988699456	26.68274642995722	30.415542919554877	21.278101661788444
31	22.857734813246402	26.315802079990036	27.744897030433386	23.081566076330173
32	23.628389804120697	28.567288300415328	26.80214705558297	21.002174839881008
33	22.95833313373349	26.56430388357421	26.898194499667067	23.57916848302523
34	21.501932924872218	26.316161359706065	29.290278848963595	22.891626866458125
35	20.810798511144856	26.55532189067358	28.94608888101135	23.687790717170216
36	22.249354494110207	27.364779090878606	28.396510675397963	21.989355739613224
37	21.53869921581214	28.55686918865059	28.632796968637276	21.271634626899992
38	21.117264108914448	27.870405411231562	26.693764341248666	24.318566138605323
39	22.7180947636179	26.667417162073477	27.49891018486139	23.115577889447238
40	23.148631623321563	24.669941700878077	28.640102322863125	23.54132435293723
41	20.80421171635106	25.83412774070543	28.342139678372796	25.01952086457071
42	21.68887813711072	26.150533410618394	28.674712935506896	23.48587551676399
43	23.025518440630226	25.845864211428925	27.47388036464496	23.654736983295887
44	21.65055496740135	26.12801854841414	28.16920637505928	24.052220109125226
45	21.411633956244522	25.245627565855973	27.662981255179613	25.67975722271989
46	21.74217129498781	27.649568145781338	26.829093034284867	23.779167524945986
47	20.642176564423643	25.629458062476346	30.57793735119832	23.150428021901693
48	20.9949892455605	25.71628399384913	29.380697577496633	23.908029183093735
49	20.56409310614081	25.238082691819443	30.225483949777487	23.972340252262264
50	19.769725654008845	25.503949681678172	30.25398680724883	24.472337857064158
51	20.52085978031243	24.9294614157537	28.55339615139568	25.996282652538195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	252.0
1	748.0
2	1244.0
3	3319.0
4	5394.0
5	3743.0
6	2092.0
7	1984.5
8	1877.0
9	1885.0
10	1893.0
11	1833.0
12	1773.0
13	1807.0
14	1841.0
15	1801.0
16	1761.0
17	1903.5
18	2046.0
19	2242.5
20	2439.0
21	2867.0
22	3295.0
23	3696.5
24	4098.0
25	4894.5
26	7344.5
27	8998.0
28	10638.0
29	12278.0
30	14442.5
31	16607.0
32	19139.0
33	21671.0
34	24775.5
35	27880.0
36	29634.5
37	31389.0
38	34173.0
39	36957.0
40	40325.0
41	43693.0
42	46350.0
43	49007.0
44	50675.5
45	52344.0
46	55241.0
47	58138.0
48	70615.5
49	83093.0
50	74151.5
51	65210.0
52	62532.5
53	59855.0
54	55044.5
55	50234.0
56	46408.0
57	42582.0
58	39662.5
59	36743.0
60	34092.5
61	31442.0
62	28160.0
63	24878.0
64	20730.0
65	16582.0
66	14187.5
67	11793.0
68	9442.0
69	7091.0
70	5708.5
71	4326.0
72	3594.5
73	2863.0
74	2335.0
75	1372.5
76	938.0
77	703.0
78	468.0
79	329.0
80	190.0
81	149.5
82	109.0
83	84.0
84	59.0
85	52.0
86	45.0
87	33.0
88	21.0
89	15.5
90	10.0
91	8.5
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	835004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.71658454663404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.91523470948371	21.667917369820376
2	8.930584342813148	5.307729293481062
3	3.786347246626979	3.375519242319168
4	2.3352778626853548	2.7758592818548875
5	1.5628726964814845	2.3221619310308976
6	1.142005452422568	2.036190094777938
7	0.8640158494516227	1.797292002790268
8	0.6877469150418137	1.6349991478021435
9	0.5427474918534535	1.4515741556223047
>10	6.885614073976902	47.63629919332884
>50	0.31471997780453764	5.615011305536973
>100	0.02802849628157932	1.4381996323160606
>500	0.002402442538421083	0.49046569739445695
>1k	0.002002035448684236	1.8332375060059187
>5k	4.004070897368472E-4	0.6175441459187848
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGC	5190	0.6215539087237906	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCG	4907	0.5876618555120694	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTC	4267	0.5110155160933361	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2802	0.3355672547676418	No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	1805	0.21616662914189633	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT	1626	0.19472960608571935	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCT	862	0.10323303840460644	No Hit
GAACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT	859	0.10287375868858113	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.790396213670833E-4	0.0	0.0	0.08622713184607499	0.0
2	4.790396213670833E-4	0.0	0.0	0.47939890108310856	0.0
3	4.790396213670833E-4	0.0	0.0	0.6001168856676136	0.0
4	4.790396213670833E-4	0.0	0.0	0.8651455561889524	0.0
5	4.790396213670833E-4	0.0	0.0	1.8324463116344354	0.0
6	4.790396213670833E-4	0.0	0.0	2.335198394259189	0.0
7	4.790396213670833E-4	0.0	0.0	2.673280607039008	0.0
8	4.790396213670833E-4	0.0	0.0	3.1622603005494585	0.0
9	4.790396213670833E-4	0.0	0.0	3.3048943478115076	0.0
10	4.790396213670833E-4	0.0	0.0	4.349799521918458	0.0
11	4.790396213670833E-4	0.0	0.0	4.941533214212147	0.0
12	4.790396213670833E-4	0.0	0.0	6.155539374661679	0.0
13	4.790396213670833E-4	0.0	0.0	6.444040986630005	0.0
14	4.790396213670833E-4	0.0	0.0	6.597932464994179	0.0
15	4.790396213670833E-4	0.0	0.0	6.8588892987338985	0.0
16	4.790396213670833E-4	0.0	0.0	7.249546109958755	0.0
17	4.790396213670833E-4	0.0	0.0	7.694693678114117	0.0
18	4.790396213670833E-4	0.0	0.0	8.163194427811124	0.0
19	4.790396213670833E-4	0.0	0.0	8.47948033781874	0.0
20	4.790396213670833E-4	0.0	0.0	8.790736331802004	0.0
21	4.790396213670833E-4	0.0	0.0	9.161992038361493	0.0
22	4.790396213670833E-4	0.0	0.0	9.571810434441032	0.0
23	4.790396213670833E-4	0.0	0.0	9.95372477257594	0.0
24	4.790396213670833E-4	0.0	0.0	10.271328041542317	0.0
25	4.790396213670833E-4	0.0	0.0	10.557793735119832	0.0
26	4.790396213670833E-4	0.0	0.0	10.844977988129399	0.0
27	4.790396213670833E-4	0.0	0.0	11.141743033566307	0.0
28	4.790396213670833E-4	0.0	0.0	11.43144224458805	0.0
29	4.790396213670833E-4	0.0	0.0	11.73838688197901	0.0
30	4.790396213670833E-4	0.0	0.0	12.102696514028676	0.0
31	4.790396213670833E-4	0.0	0.0	12.429401535801025	0.0
32	4.790396213670833E-4	0.0	0.0	12.747244324578086	0.0
33	4.790396213670833E-4	0.0	0.0	13.068919430326082	0.0
34	4.790396213670833E-4	0.0	0.0	13.401851967176205	0.0
35	5.987995267088541E-4	0.0	0.0	13.74975449219405	0.0
36	5.987995267088541E-4	0.0	0.0	14.088914544121945	0.0
37	5.987995267088541E-4	0.0	0.0	14.440050586584016	0.0
38	7.185594320506249E-4	0.0	0.0	14.79130638895143	0.0
39	8.383193373923957E-4	0.0	0.0	15.227711484016844	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAT	25	3.8898856E-5	45.0	27
TCGCGCA	25	3.8898856E-5	45.0	34
TAGTACG	25	3.8898856E-5	45.0	1
ATTAGCG	30	2.1647866E-6	44.999996	1
CGACGGT	100	0.0	42.75	28
TTAGCGG	130	0.0	41.53846	2
ACGTGCG	65	0.0	41.53846	1
TACGGCT	640	0.0	41.484375	7
CGCATAG	55	6.002665E-11	40.909092	2
CGGTCTA	55	6.002665E-11	40.909092	31
GCTTGCG	45	1.9272193E-8	40.0	1
GTTAGCG	40	3.4567165E-7	39.375	1
CGGGATA	195	0.0	39.23077	6
CGTTTTT	1485	0.0	38.939396	1
TACGGGA	215	0.0	38.720932	4
CTATCGA	35	6.246235E-6	38.57143	14
ACGGAAC	35	6.246235E-6	38.57143	40
TCTATCG	35	6.246235E-6	38.57143	13
TCACGAC	65	9.094947E-12	38.07692	25
CGCTCAT	65	9.094947E-12	38.07692	13
>>END_MODULE