##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936624.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	652731
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.184968999480645	31.0	31.0	33.0	28.0	34.0
2	31.222561821025813	31.0	31.0	34.0	28.0	34.0
3	30.992148373526	31.0	31.0	34.0	28.0	34.0
4	34.972665615697736	35.0	35.0	37.0	32.0	37.0
5	35.207888088661335	35.0	35.0	37.0	33.0	37.0
6	35.18601843638498	36.0	35.0	37.0	32.0	37.0
7	30.96304909679485	35.0	35.0	37.0	0.0	37.0
8	33.05565692452174	36.0	35.0	37.0	17.0	37.0
9	36.3831226033389	39.0	35.0	39.0	32.0	39.0
10	36.13936828494433	37.0	35.0	39.0	32.0	39.0
11	36.376622222630765	38.0	35.0	39.0	32.0	39.0
12	36.42944490149847	38.0	35.0	39.0	32.0	39.0
13	36.355826519653576	39.0	35.0	39.0	32.0	39.0
14	37.40342652639449	39.0	36.0	41.0	32.0	41.0
15	37.63833033822509	39.0	36.0	41.0	33.0	41.0
16	37.57061025138993	39.0	36.0	41.0	33.0	41.0
17	37.502058275154695	39.0	36.0	41.0	32.0	41.0
18	37.398681845967175	39.0	36.0	40.0	32.0	41.0
19	37.21349683100696	39.0	36.0	40.0	32.0	41.0
20	37.21101341900415	39.0	35.0	40.0	32.0	41.0
21	37.208062739474606	39.0	35.0	40.0	32.0	41.0
22	37.290239011169994	39.0	35.0	40.0	32.0	41.0
23	37.34218843597132	39.0	35.0	40.0	32.0	41.0
24	37.27704061857028	39.0	35.0	41.0	32.0	41.0
25	36.95370374625995	39.0	35.0	40.0	31.0	41.0
26	36.94888859269745	39.0	35.0	40.0	31.0	41.0
27	36.96285606168544	39.0	35.0	40.0	31.0	41.0
28	36.95358118428572	39.0	35.0	40.0	31.0	41.0
29	36.961831137175956	39.0	35.0	40.0	31.0	41.0
30	36.74677930112098	39.0	35.0	40.0	31.0	41.0
31	36.729170209473736	39.0	35.0	40.0	31.0	41.0
32	36.60174405689327	39.0	35.0	40.0	31.0	41.0
33	36.47251011519293	39.0	35.0	40.0	30.0	41.0
34	36.42560871170513	39.0	35.0	40.0	30.0	41.0
35	36.38144197226729	39.0	35.0	40.0	30.0	41.0
36	36.30150552065093	39.0	35.0	40.0	30.0	41.0
37	36.25146346657352	39.0	35.0	40.0	30.0	41.0
38	36.1450842690174	39.0	35.0	40.0	30.0	41.0
39	36.108893250052475	39.0	35.0	40.0	30.0	41.0
40	36.024978130347726	38.0	35.0	40.0	29.0	41.0
41	35.87703050720741	38.0	35.0	40.0	29.0	41.0
42	35.87851350709557	38.0	35.0	40.0	29.0	41.0
43	35.84564851370626	38.0	35.0	40.0	29.0	41.0
44	35.669018324547174	38.0	35.0	40.0	28.0	41.0
45	35.59680480933187	38.0	35.0	40.0	28.0	41.0
46	35.49748824554066	38.0	34.0	40.0	28.0	41.0
47	35.46750345854571	38.0	34.0	40.0	28.0	41.0
48	35.40641550654098	38.0	34.0	40.0	28.0	41.0
49	35.27498004537857	38.0	34.0	40.0	27.0	41.0
50	35.150195103342725	38.0	34.0	40.0	27.0	41.0
51	33.53392898452808	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	26.0
9	58.0
10	83.0
11	65.0
12	37.0
13	34.0
14	44.0
15	53.0
16	102.0
17	147.0
18	342.0
19	590.0
20	935.0
21	1413.0
22	2066.0
23	2926.0
24	4133.0
25	5750.0
26	7571.0
27	8735.0
28	10009.0
29	11344.0
30	14007.0
31	17788.0
32	23384.0
33	32526.0
34	51846.0
35	55752.0
36	63793.0
37	96779.0
38	130633.0
39	109751.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.29255543248291	22.229678075654444	24.86843738078933	12.609329111073322
2	34.269094006566256	24.251644245485508	24.236170796239186	17.24309095170905
3	32.55460518958039	23.663040364254186	26.720961621249796	17.061392824915625
4	29.655708094145982	25.070971043201563	25.591706231203972	19.681614631448483
5	26.108458155043962	29.94740559280929	24.463829663368216	19.480306588778532
6	27.500302574873874	34.39380081534353	23.94646492965709	14.159431680125504
7	68.77013654935953	18.45216482747104	8.869044062561759	3.908654560607662
8	80.384415632167	6.085968032773072	9.183568728925087	4.34604760613484
9	74.0404546436434	8.403614965429863	11.472260395170446	6.083669995756292
10	42.305176251779066	25.741231839762474	17.982752466176724	13.970839442281735
11	32.05225429771223	26.637466276306775	24.26374724044055	17.046532185540446
12	30.566190360194323	23.01039785148859	27.835509574388222	18.58790221392886
13	25.576385984425436	25.28943776226347	28.966450191579685	20.167726061731404
14	19.941139611876867	28.593095777586786	30.066137505342937	21.39962710519341
15	19.560891086833625	26.91675437507947	34.26434473006491	19.258009808021985
16	21.78753575362592	25.353476393797752	33.25474046735945	19.60424738521688
17	22.67273961248968	25.029299971963948	29.173886332961054	23.124074082585324
18	23.522706903762806	26.667647162460494	29.16331536268386	20.64633057109284
19	25.21222371849966	27.689967229992142	26.970222036336562	20.127587015171642
20	27.14916251870985	25.863947016458543	27.97461741513732	19.012273049694283
21	24.898311862007475	26.54952805979799	28.766520971119803	19.785639107074736
22	23.64787331994344	24.984258446435057	28.356704369793988	23.01116386382752
23	23.271608059062615	26.515976719352995	28.8575232369843	21.354891984600087
24	23.185508272167247	24.953924357813555	29.726947241666167	22.133620128353027
25	22.76849115485552	25.996160746157297	28.55663359025387	22.678714508733304
26	21.529696000343172	28.203195497073068	28.015828878971583	22.251279623612177
27	21.606144031768064	28.33663484651411	29.045655867424713	21.011565254293117
28	20.088673588354162	27.901080230600357	30.679866591291056	21.33037958975443
29	21.162776089997255	26.486408643070426	30.092028722398663	22.25878654453366
30	22.001559601122057	26.507397381157016	29.856403326944793	21.63463969077614
31	23.080564581734283	28.09671978196225	26.814874734002213	22.007840902301254
32	22.924910874464366	27.750329002299566	27.41389638304294	21.910863740193125
33	23.035063448802035	29.02543314167705	26.46511350004826	21.474389909472663
34	21.329460374947722	27.73378313577875	28.725309507285544	22.211446981987987
35	21.611659320608336	27.03058380864399	27.621945334295443	23.73581153645223
36	21.92097510306696	29.360640141191396	27.539216001691358	21.17916875405029
37	21.787688956093703	29.423912760386745	28.03896245160717	20.74943583191238
38	21.47546232674716	28.97916599640587	26.414250280743524	23.13112139610345
39	22.068815484479824	27.625622193522293	27.17290890121658	23.132653420781303
40	22.454885703298906	26.22887529472325	29.57527679855867	21.740962203419173
41	19.836042718976117	27.37620857596774	29.103872805183144	23.683875899872994
42	20.584743179043127	28.071441374777667	27.8388800286795	23.504935417499706
43	22.118299881574494	27.266056001630073	27.40945351147716	23.20619060531827
44	21.575656740678777	27.327183786276425	27.266056001630073	23.831103471414718
45	21.605071614493564	26.87110003967944	27.00453938912048	24.519288956706514
46	21.055074755144158	28.211928037736833	27.100903741357467	23.632093465761546
47	21.320727834283954	27.37513615869324	29.191657819224154	22.112478187798647
48	20.890228899807116	25.55555044880663	29.722963977503746	23.831256673882503
49	19.84615408184995	26.73000056684913	29.558424527102282	23.865420824198637
50	19.3185247827972	26.681741789496744	29.792671100346084	24.207062327359967
51	19.151534092911167	26.549221654862414	28.2059531414932	26.09329111073321
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	212.0
1	740.5
2	1269.0
3	3643.5
4	6018.0
5	4192.5
6	2367.0
7	2229.0
8	2091.0
9	1997.0
10	1903.0
11	1872.0
12	1841.0
13	1771.5
14	1702.0
15	1740.0
16	1778.0
17	1793.5
18	1809.0
19	1877.0
20	1945.0
21	2281.0
22	2617.0
23	2908.0
24	3199.0
25	4266.5
26	5986.0
27	6638.0
28	8055.5
29	9473.0
30	11322.5
31	13172.0
32	15378.5
33	17585.0
34	18885.0
35	20185.0
36	21412.0
37	22639.0
38	25014.0
39	27389.0
40	30166.0
41	32943.0
42	37692.5
43	42442.0
44	46937.5
45	51433.0
46	52771.0
47	54109.0
48	52716.0
49	51323.0
50	47159.0
51	42995.0
52	38838.0
53	34681.0
54	33616.5
55	32552.0
56	32193.5
57	31835.0
58	32044.5
59	32254.0
60	31032.5
61	29811.0
62	26299.5
63	22788.0
64	19221.5
65	15655.0
66	12650.0
67	9645.0
68	7882.5
69	6120.0
70	5075.0
71	4030.0
72	3404.0
73	2778.0
74	2446.0
75	1547.5
76	981.0
77	724.5
78	468.0
79	373.5
80	279.0
81	212.0
82	145.0
83	112.5
84	80.0
85	55.0
86	30.0
87	25.5
88	21.0
89	19.0
90	17.0
91	15.0
92	13.0
93	16.5
94	20.0
95	11.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	652731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.922881089862884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.90905769548012	22.11571944885667
2	9.040322625163558	5.410249978535324
3	3.6289756965830993	3.257682247405752
4	2.1080405062056715	2.523145815992266
5	1.4191354076271163	2.1232310026420143
6	1.1122962808493793	1.9969865609131627
7	0.830652121940181	1.7398853270300823
8	0.6325113381368025	1.5141249247246076
9	0.4887870070062382	1.3163323940025875
>10	6.307430901882276	45.842489205408576
>50	0.4770208299025805	8.648454271296751
>100	0.0427182832748008	2.3844004359578523
>500	0.002542754956833381	0.6520617696138489
>1k	5.085509913666763E-4	0.4752366176205484
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3123	0.47845130689365145	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC	949	0.14538914192829816	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG	939	0.14385711725044467	No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	930	0.1424782950403765	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGC	856	0.13114131242426053	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07031993271347615	0.0
2	0.0	0.0	0.0	0.42023436913521806	0.0
3	0.0	0.0	0.0	0.586459046682324	0.0
4	0.0	0.0	0.0	0.8415411555449335	0.0
5	0.0	0.0	0.0	1.5884031859985202	0.0
6	0.0	0.0	0.0	2.0023562539545385	0.0
7	0.0	0.0	0.0	2.345836186729296	0.0
8	0.0	0.0	0.0	2.9391893444619606	0.0
9	0.0	0.0	0.0	3.1355949081627807	0.0
10	0.0	0.0	0.0	3.764644240889432	0.0
11	0.0	0.0	0.0	4.5488876734826444	0.0
12	0.0	0.0	0.0	5.2758333831241355	0.0
13	0.0	0.0	0.0	5.579480674274701	0.0
14	0.0	0.0	0.0	5.723031386589575	0.0
15	0.0	0.0	0.0	5.952528683332031	0.0
16	0.0	0.0	0.0	6.411216871881372	0.0
17	0.0	0.0	0.0	6.893804645405228	0.0
18	0.0	0.0	0.0	7.483327741443259	0.0
19	0.0	0.0	0.0	7.84779641230461	0.0
20	0.0	0.0	0.0	8.204758162244477	0.0
21	0.0	0.0	0.0	8.654101000258912	0.0
22	0.0	0.0	0.0	9.099920181514284	0.0
23	0.0	0.0	0.0	9.546964982511938	0.0
24	0.0	0.0	0.0	9.8883000807377	0.0
25	0.0	0.0	0.0	10.215693754394996	0.0
26	0.0	0.0	0.0	10.505093216041525	0.0
27	0.0	0.0	0.0	10.796637512237048	0.0
28	0.0	0.0	0.0	11.105493687292315	0.0
29	0.0	0.0	0.0	11.427831679512694	0.0
30	0.0	0.0	0.0	11.811757063782784	0.0
31	0.0	0.0	0.0	12.183579453097831	0.0
32	0.0	0.0	0.0	12.541766822779982	0.0
33	1.5320246778535108E-4	0.0	0.0	12.871918140857414	0.0
34	1.5320246778535108E-4	0.0	0.0	13.218462122987876	0.0
35	1.5320246778535108E-4	0.0	0.0	13.614030894809654	0.0
36	1.5320246778535108E-4	0.0	0.0	13.979418780477715	0.0
37	1.5320246778535108E-4	0.0	0.0	14.342508629128998	0.0
38	1.5320246778535108E-4	0.0	0.0	14.724901988721234	0.0
39	1.5320246778535108E-4	0.0	0.0	15.108674170523539	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCATGCG	20	7.031043E-4	45.000004	1
TTGCGTC	20	7.031043E-4	45.000004	13
CGATGCA	20	7.031043E-4	45.000004	10
CGCGTAA	20	7.031043E-4	45.000004	31
TCGAATT	20	7.031043E-4	45.000004	31
TCGCGTA	20	7.031043E-4	45.000004	30
TCGTTCA	25	3.888947E-5	45.0	16
TCCGATT	25	3.888947E-5	45.0	21
TGCGATG	25	3.888947E-5	45.0	1
TTCGAAT	25	3.888947E-5	45.0	32
TAATCCG	25	3.888947E-5	45.0	18
TGCGCAA	30	2.1640553E-6	44.999996	1
CTAGTCG	30	2.1640553E-6	44.999996	1
CGTCATA	60	0.0	44.999996	38
CGAATAT	115	0.0	43.043476	14
TACGGGT	70	0.0	41.785717	4
TACGAAT	120	0.0	41.249996	12
GCTACGA	120	0.0	41.249996	10
TGTTACG	50	1.0804797E-9	40.5	1
CGGGTAT	90	0.0	40.0	6
>>END_MODULE